Variable gp43 secretion by Paracoccidioides brasiliensis clones obtained by two different culture methods

Detalhes bibliográficos
Autor(a) principal: Berzaghi, Rodrigo [UNIFESP]
Data de Publicação: 2005
Outros Autores: Silva, Silvia Helena M da [UNIFESP], Camargo, Zoilo Pires de [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://repositorio.unifesp.br/handle/11600/28063
http://dx.doi.org/10.1128/JCM.43.1.491-493.2005
Resumo: The main objectives of this study were to obtain clones of Paracoccidioides brasiliensis by two methods (micromanipulation and plating assay) and to determine if the secretion of the 43-kDa glycoprotein (gp43) is dependent on the clonal culture. the results show that the secretion of gp43 is not dependent on clonal cultures. Clones that originally were secretors of this molecule, after subculturing, lost this characteristic; on the other hand, clones that originally did not secrete gp43 began to secrete gp43 after subculturing.
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spelling Berzaghi, Rodrigo [UNIFESP]Silva, Silvia Helena M da [UNIFESP]Camargo, Zoilo Pires de [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2016-01-24T12:37:32Z2016-01-24T12:37:32Z2005-01-01Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 43, n. 1, p. 491-493, 2005.0095-1137http://repositorio.unifesp.br/handle/11600/28063http://dx.doi.org/10.1128/JCM.43.1.491-493.2005WOS000226380400080.pdf10.1128/JCM.43.1.491-493.2005WOS:000226380400080The main objectives of this study were to obtain clones of Paracoccidioides brasiliensis by two methods (micromanipulation and plating assay) and to determine if the secretion of the 43-kDa glycoprotein (gp43) is dependent on the clonal culture. the results show that the secretion of gp43 is not dependent on clonal cultures. Clones that originally were secretors of this molecule, after subculturing, lost this characteristic; on the other hand, clones that originally did not secrete gp43 began to secrete gp43 after subculturing.Universidade Federal de São Paulo, Disciplina Biol Celular, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Disciplina Biol Celular, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, BrazilWeb of Science491-493engAmer Soc MicrobiologyJournal of Clinical MicrobiologyVariable gp43 secretion by Paracoccidioides brasiliensis clones obtained by two different culture methodsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000226380400080.pdfapplication/pdf94128${dspace.ui.url}/bitstream/11600/28063/1/WOS000226380400080.pdff37f05c1507e2c5b24dcf87c79a1f451MD51open accessTEXTWOS000226380400080.pdf.txtWOS000226380400080.pdf.txtExtracted texttext/plain17748${dspace.ui.url}/bitstream/11600/28063/2/WOS000226380400080.pdf.txtd53c6e8760a08a2d7feed74ed84d61c2MD52open access11600/280632022-02-18 12:05:30.181open accessoai:repositorio.unifesp.br:11600/28063Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-02-18T15:05:30Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.en.fl_str_mv Variable gp43 secretion by Paracoccidioides brasiliensis clones obtained by two different culture methods
title Variable gp43 secretion by Paracoccidioides brasiliensis clones obtained by two different culture methods
spellingShingle Variable gp43 secretion by Paracoccidioides brasiliensis clones obtained by two different culture methods
Berzaghi, Rodrigo [UNIFESP]
title_short Variable gp43 secretion by Paracoccidioides brasiliensis clones obtained by two different culture methods
title_full Variable gp43 secretion by Paracoccidioides brasiliensis clones obtained by two different culture methods
title_fullStr Variable gp43 secretion by Paracoccidioides brasiliensis clones obtained by two different culture methods
title_full_unstemmed Variable gp43 secretion by Paracoccidioides brasiliensis clones obtained by two different culture methods
title_sort Variable gp43 secretion by Paracoccidioides brasiliensis clones obtained by two different culture methods
author Berzaghi, Rodrigo [UNIFESP]
author_facet Berzaghi, Rodrigo [UNIFESP]
Silva, Silvia Helena M da [UNIFESP]
Camargo, Zoilo Pires de [UNIFESP]
author_role author
author2 Silva, Silvia Helena M da [UNIFESP]
Camargo, Zoilo Pires de [UNIFESP]
author2_role author
author
dc.contributor.institution.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Berzaghi, Rodrigo [UNIFESP]
Silva, Silvia Helena M da [UNIFESP]
Camargo, Zoilo Pires de [UNIFESP]
description The main objectives of this study were to obtain clones of Paracoccidioides brasiliensis by two methods (micromanipulation and plating assay) and to determine if the secretion of the 43-kDa glycoprotein (gp43) is dependent on the clonal culture. the results show that the secretion of gp43 is not dependent on clonal cultures. Clones that originally were secretors of this molecule, after subculturing, lost this characteristic; on the other hand, clones that originally did not secrete gp43 began to secrete gp43 after subculturing.
publishDate 2005
dc.date.issued.fl_str_mv 2005-01-01
dc.date.accessioned.fl_str_mv 2016-01-24T12:37:32Z
dc.date.available.fl_str_mv 2016-01-24T12:37:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 43, n. 1, p. 491-493, 2005.
dc.identifier.uri.fl_str_mv http://repositorio.unifesp.br/handle/11600/28063
http://dx.doi.org/10.1128/JCM.43.1.491-493.2005
dc.identifier.issn.none.fl_str_mv 0095-1137
dc.identifier.file.none.fl_str_mv WOS000226380400080.pdf
dc.identifier.doi.none.fl_str_mv 10.1128/JCM.43.1.491-493.2005
dc.identifier.wos.none.fl_str_mv WOS:000226380400080
identifier_str_mv Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 43, n. 1, p. 491-493, 2005.
0095-1137
WOS000226380400080.pdf
10.1128/JCM.43.1.491-493.2005
WOS:000226380400080
url http://repositorio.unifesp.br/handle/11600/28063
http://dx.doi.org/10.1128/JCM.43.1.491-493.2005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv Journal of Clinical Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 491-493
dc.publisher.none.fl_str_mv Amer Soc Microbiology
publisher.none.fl_str_mv Amer Soc Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
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