Variable gp43 secretion by Paracoccidioides brasiliensis clones obtained by two different culture methods
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/28063 http://dx.doi.org/10.1128/JCM.43.1.491-493.2005 |
Resumo: | The main objectives of this study were to obtain clones of Paracoccidioides brasiliensis by two methods (micromanipulation and plating assay) and to determine if the secretion of the 43-kDa glycoprotein (gp43) is dependent on the clonal culture. the results show that the secretion of gp43 is not dependent on clonal cultures. Clones that originally were secretors of this molecule, after subculturing, lost this characteristic; on the other hand, clones that originally did not secrete gp43 began to secrete gp43 after subculturing. |
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Berzaghi, Rodrigo [UNIFESP]Silva, Silvia Helena M da [UNIFESP]Camargo, Zoilo Pires de [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2016-01-24T12:37:32Z2016-01-24T12:37:32Z2005-01-01Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 43, n. 1, p. 491-493, 2005.0095-1137http://repositorio.unifesp.br/handle/11600/28063http://dx.doi.org/10.1128/JCM.43.1.491-493.2005WOS000226380400080.pdf10.1128/JCM.43.1.491-493.2005WOS:000226380400080The main objectives of this study were to obtain clones of Paracoccidioides brasiliensis by two methods (micromanipulation and plating assay) and to determine if the secretion of the 43-kDa glycoprotein (gp43) is dependent on the clonal culture. the results show that the secretion of gp43 is not dependent on clonal cultures. Clones that originally were secretors of this molecule, after subculturing, lost this characteristic; on the other hand, clones that originally did not secrete gp43 began to secrete gp43 after subculturing.Universidade Federal de São Paulo, Disciplina Biol Celular, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Disciplina Biol Celular, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, BrazilWeb of Science491-493engAmer Soc MicrobiologyJournal of Clinical MicrobiologyVariable gp43 secretion by Paracoccidioides brasiliensis clones obtained by two different culture methodsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000226380400080.pdfapplication/pdf94128${dspace.ui.url}/bitstream/11600/28063/1/WOS000226380400080.pdff37f05c1507e2c5b24dcf87c79a1f451MD51open accessTEXTWOS000226380400080.pdf.txtWOS000226380400080.pdf.txtExtracted texttext/plain17748${dspace.ui.url}/bitstream/11600/28063/2/WOS000226380400080.pdf.txtd53c6e8760a08a2d7feed74ed84d61c2MD52open access11600/280632022-02-18 12:05:30.181open accessoai:repositorio.unifesp.br:11600/28063Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-02-18T15:05:30Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Variable gp43 secretion by Paracoccidioides brasiliensis clones obtained by two different culture methods |
title |
Variable gp43 secretion by Paracoccidioides brasiliensis clones obtained by two different culture methods |
spellingShingle |
Variable gp43 secretion by Paracoccidioides brasiliensis clones obtained by two different culture methods Berzaghi, Rodrigo [UNIFESP] |
title_short |
Variable gp43 secretion by Paracoccidioides brasiliensis clones obtained by two different culture methods |
title_full |
Variable gp43 secretion by Paracoccidioides brasiliensis clones obtained by two different culture methods |
title_fullStr |
Variable gp43 secretion by Paracoccidioides brasiliensis clones obtained by two different culture methods |
title_full_unstemmed |
Variable gp43 secretion by Paracoccidioides brasiliensis clones obtained by two different culture methods |
title_sort |
Variable gp43 secretion by Paracoccidioides brasiliensis clones obtained by two different culture methods |
author |
Berzaghi, Rodrigo [UNIFESP] |
author_facet |
Berzaghi, Rodrigo [UNIFESP] Silva, Silvia Helena M da [UNIFESP] Camargo, Zoilo Pires de [UNIFESP] |
author_role |
author |
author2 |
Silva, Silvia Helena M da [UNIFESP] Camargo, Zoilo Pires de [UNIFESP] |
author2_role |
author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Berzaghi, Rodrigo [UNIFESP] Silva, Silvia Helena M da [UNIFESP] Camargo, Zoilo Pires de [UNIFESP] |
description |
The main objectives of this study were to obtain clones of Paracoccidioides brasiliensis by two methods (micromanipulation and plating assay) and to determine if the secretion of the 43-kDa glycoprotein (gp43) is dependent on the clonal culture. the results show that the secretion of gp43 is not dependent on clonal cultures. Clones that originally were secretors of this molecule, after subculturing, lost this characteristic; on the other hand, clones that originally did not secrete gp43 began to secrete gp43 after subculturing. |
publishDate |
2005 |
dc.date.issued.fl_str_mv |
2005-01-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T12:37:32Z |
dc.date.available.fl_str_mv |
2016-01-24T12:37:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 43, n. 1, p. 491-493, 2005. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/28063 http://dx.doi.org/10.1128/JCM.43.1.491-493.2005 |
dc.identifier.issn.none.fl_str_mv |
0095-1137 |
dc.identifier.file.none.fl_str_mv |
WOS000226380400080.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1128/JCM.43.1.491-493.2005 |
dc.identifier.wos.none.fl_str_mv |
WOS:000226380400080 |
identifier_str_mv |
Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 43, n. 1, p. 491-493, 2005. 0095-1137 WOS000226380400080.pdf 10.1128/JCM.43.1.491-493.2005 WOS:000226380400080 |
url |
http://repositorio.unifesp.br/handle/11600/28063 http://dx.doi.org/10.1128/JCM.43.1.491-493.2005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Journal of Clinical Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
491-493 |
dc.publisher.none.fl_str_mv |
Amer Soc Microbiology |
publisher.none.fl_str_mv |
Amer Soc Microbiology |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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Universidade Federal de São Paulo (UNIFESP) |
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UNIFESP |
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UNIFESP |
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Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
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