Avaliação de um modelo experimental de enterocolite necrosante neonatal em ratos

• Autor(a) principal: Meyer, Karine Furtado [UNIFESP]
• Data de Publicação: 2006
• Outros Autores: Martins, José Luiz [UNIFESP], Freitas Filho, Luiz Gonzaga de [UNIFESP], Oliva, Maria Luiza Vilela [UNIFESP], Patricio, Francy Reis da Silva [UNIFESP], Macedo, Mauricio [UNIFESP], Wang, Lina
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.1590/S0102-86502006000200011; http://repositorio.unifesp.br/handle/11600/3015
• topic: Enterocolitis, Necrotizing; Models, Animal; Rats; Enterocolite Necrosante; Modelos Animais; Ratos

OBJECTIVE: To evaluate an experimental model of necrotizing enterocolitis in rats proposed by OKUR e col. in 1995. METHODS: On their first day of life, 28 EPM-Wistar rats weighing between 4 and 6 grams were submitted to hypoxia (H) by placing them in a CO2 gas chamber for rodents' sacrifice, where they received a 100% CO2 air flow for 5 minutes. After the hypoxia the animals were reanimated (R) with a 100% O2 air flow, also for 5 minutes. The animals were allocated in two groups: G1: control (n=12): rats not submitted to H-R; G2: (n=16): rats submitted to H-R. Segments of the small intestine and colon were prepared for histological analysis. The remaining intestine was used to measure tissular malondialdehyde. RESULTS: Mean malondialdehyde dosages were 1.05 (0.44-2.03) and 2.60 (0.59- 6.4) nmol MDA/mg protein for G1 and G2, respectively. G2's mean value was significantly higher than in the control group (p<0.002). Significant statistical difference between the studied groups was found in relation the level of injury, with G1 presenting significantly lower levels than G2. CONCLUSIONS: The model showed that neonatal hypoxia may cause intestinal wall injury in rats. Despite the discreet histological injuries found, the method is suitable for evaluation of tissular free radicals.