Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord Units: Cord is Different from Blood

Detalhes bibliográficos
Autor(a) principal: Secco, Mariane
Data de Publicação: 2009
Outros Autores: Moreira, Yuri B., Zucconi, Eder, Vieira, Natassia M., Jazedje, Tatiana, Muotri, Alysson R., Okamoto, Oswaldo Keith [UNIFESP], Verjovski-Almeida, Sergio, Zatz, Mayana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
dARK ID: ark:/48912/001300000xhrs
DOI: 10.1007/s12015-009-9098-5
Texto Completo: http://dx.doi.org/10.1007/s12015-009-9098-5
http://repositorio.unifesp.br/handle/11600/31956
Resumo: Mesenchymal stem cells (MSC) are multipotent cells which can be obtained from several adult and fetal tissues including human umbilical cord units. We have recently shown that umbilical cord tissue (UC) is richer in MSC than umbilical cord blood (UCB) but their origin and characteristics in blood as compared to the cord remains unknown. Here we compared, for the first time, the exonic protein-coding and intronic noncoding RNA (ncRNA) expression profiles of MSC from match-paired UC and UCB samples, harvested from the same donors, processed simultaneously and under the same culture conditions. the patterns of intronic ncRNA expression in MSC from UC and UCB paired units were highly similar, indicative of their common donor origin. the respective exonic protein-coding transcript expression profiles, however, were significantly different. Hierarchical clustering based on protein-coding expression similarities grouped MSC according to their tissue location rather than original donor. Genes related to systems development, osteogenesis and immune system were expressed at higher levels in UCB, whereas genes related to cell adhesion, morphogenesis, secretion, angiogenesis and neurogenesis were more expressed in UC cells. These molecular differences verified in tissue-specific MSC gene expression may reflect functional activities influenced by distinct niches and should be considered when developing clinical protocols involving MSC from different sources. in addition, these findings reinforce our previous suggestion on the importance of banking the whole umbilical cord unit for research or future therapeutic use.
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spelling Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord Units: Cord is Different from BloodHuman umbilical cordHuman umbilical cord bloodMesenchymal stem cellsComparative gene expression profileMesenchymal stem cells (MSC) are multipotent cells which can be obtained from several adult and fetal tissues including human umbilical cord units. We have recently shown that umbilical cord tissue (UC) is richer in MSC than umbilical cord blood (UCB) but their origin and characteristics in blood as compared to the cord remains unknown. Here we compared, for the first time, the exonic protein-coding and intronic noncoding RNA (ncRNA) expression profiles of MSC from match-paired UC and UCB samples, harvested from the same donors, processed simultaneously and under the same culture conditions. the patterns of intronic ncRNA expression in MSC from UC and UCB paired units were highly similar, indicative of their common donor origin. the respective exonic protein-coding transcript expression profiles, however, were significantly different. Hierarchical clustering based on protein-coding expression similarities grouped MSC according to their tissue location rather than original donor. Genes related to systems development, osteogenesis and immune system were expressed at higher levels in UCB, whereas genes related to cell adhesion, morphogenesis, secretion, angiogenesis and neurogenesis were more expressed in UC cells. These molecular differences verified in tissue-specific MSC gene expression may reflect functional activities influenced by distinct niches and should be considered when developing clinical protocols involving MSC from different sources. in addition, these findings reinforce our previous suggestion on the importance of banking the whole umbilical cord unit for research or future therapeutic use.Univ São Paulo, Dept Genet & Evolut Biol, Human Genome Res Ctr, BR-05508090 São Paulo, BrazilUniv São Paulo, Inst Quim, Dept Bioquim, BR-05508900 São Paulo, BrazilUniv Calif San Diego, Dept Pediat & Cellular & Mol Biol, UCSD Stem Cell Initiat, La Jolla, CA 92093 USAUniversidade Federal de São Paulo, Dept Neurol & Neurosurg, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Neurol & Neurosurg, BR-04023900 São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)INCT (Instituto Nacional de Ciencia e Tecnologia)SpringerUniversidade de São Paulo (USP)Univ Calif San DiegoUniversidade Federal de São Paulo (UNIFESP)Secco, MarianeMoreira, Yuri B.Zucconi, EderVieira, Natassia M.Jazedje, TatianaMuotri, Alysson R.Okamoto, Oswaldo Keith [UNIFESP]Verjovski-Almeida, SergioZatz, Mayana2016-01-24T13:58:55Z2016-01-24T13:58:55Z2009-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion387-401application/pdfhttp://dx.doi.org/10.1007/s12015-009-9098-5Stem Cell Reviews and Reports. Heidelberg: Springer Heidelberg, v. 5, n. 4, p. 387-401, 2009.10.1007/s12015-009-9098-5WOS000273470400010.pdf1550-8943http://repositorio.unifesp.br/handle/11600/31956WOS:000273470400010ark:/48912/001300000xhrsengStem Cell Reviews and Reportsinfo:eu-repo/semantics/openAccesshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-07T21:45:53Zoai:repositorio.unifesp.br/:11600/31956Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T20:43:25.544398Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord Units: Cord is Different from Blood
title Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord Units: Cord is Different from Blood
spellingShingle Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord Units: Cord is Different from Blood
Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord Units: Cord is Different from Blood
Secco, Mariane
Human umbilical cord
Human umbilical cord blood
Mesenchymal stem cells
Comparative gene expression profile
Secco, Mariane
Human umbilical cord
Human umbilical cord blood
Mesenchymal stem cells
Comparative gene expression profile
title_short Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord Units: Cord is Different from Blood
title_full Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord Units: Cord is Different from Blood
title_fullStr Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord Units: Cord is Different from Blood
Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord Units: Cord is Different from Blood
title_full_unstemmed Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord Units: Cord is Different from Blood
Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord Units: Cord is Different from Blood
title_sort Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord Units: Cord is Different from Blood
author Secco, Mariane
author_facet Secco, Mariane
Secco, Mariane
Moreira, Yuri B.
Zucconi, Eder
Vieira, Natassia M.
Jazedje, Tatiana
Muotri, Alysson R.
Okamoto, Oswaldo Keith [UNIFESP]
Verjovski-Almeida, Sergio
Zatz, Mayana
Moreira, Yuri B.
Zucconi, Eder
Vieira, Natassia M.
Jazedje, Tatiana
Muotri, Alysson R.
Okamoto, Oswaldo Keith [UNIFESP]
Verjovski-Almeida, Sergio
Zatz, Mayana
author_role author
author2 Moreira, Yuri B.
Zucconi, Eder
Vieira, Natassia M.
Jazedje, Tatiana
Muotri, Alysson R.
Okamoto, Oswaldo Keith [UNIFESP]
Verjovski-Almeida, Sergio
Zatz, Mayana
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Univ Calif San Diego
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Secco, Mariane
Moreira, Yuri B.
Zucconi, Eder
Vieira, Natassia M.
Jazedje, Tatiana
Muotri, Alysson R.
Okamoto, Oswaldo Keith [UNIFESP]
Verjovski-Almeida, Sergio
Zatz, Mayana
dc.subject.por.fl_str_mv Human umbilical cord
Human umbilical cord blood
Mesenchymal stem cells
Comparative gene expression profile
topic Human umbilical cord
Human umbilical cord blood
Mesenchymal stem cells
Comparative gene expression profile
description Mesenchymal stem cells (MSC) are multipotent cells which can be obtained from several adult and fetal tissues including human umbilical cord units. We have recently shown that umbilical cord tissue (UC) is richer in MSC than umbilical cord blood (UCB) but their origin and characteristics in blood as compared to the cord remains unknown. Here we compared, for the first time, the exonic protein-coding and intronic noncoding RNA (ncRNA) expression profiles of MSC from match-paired UC and UCB samples, harvested from the same donors, processed simultaneously and under the same culture conditions. the patterns of intronic ncRNA expression in MSC from UC and UCB paired units were highly similar, indicative of their common donor origin. the respective exonic protein-coding transcript expression profiles, however, were significantly different. Hierarchical clustering based on protein-coding expression similarities grouped MSC according to their tissue location rather than original donor. Genes related to systems development, osteogenesis and immune system were expressed at higher levels in UCB, whereas genes related to cell adhesion, morphogenesis, secretion, angiogenesis and neurogenesis were more expressed in UC cells. These molecular differences verified in tissue-specific MSC gene expression may reflect functional activities influenced by distinct niches and should be considered when developing clinical protocols involving MSC from different sources. in addition, these findings reinforce our previous suggestion on the importance of banking the whole umbilical cord unit for research or future therapeutic use.
publishDate 2009
dc.date.none.fl_str_mv 2009-12-01
2016-01-24T13:58:55Z
2016-01-24T13:58:55Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s12015-009-9098-5
Stem Cell Reviews and Reports. Heidelberg: Springer Heidelberg, v. 5, n. 4, p. 387-401, 2009.
10.1007/s12015-009-9098-5
WOS000273470400010.pdf
1550-8943
http://repositorio.unifesp.br/handle/11600/31956
WOS:000273470400010
dc.identifier.dark.fl_str_mv ark:/48912/001300000xhrs
url http://dx.doi.org/10.1007/s12015-009-9098-5
http://repositorio.unifesp.br/handle/11600/31956
identifier_str_mv Stem Cell Reviews and Reports. Heidelberg: Springer Heidelberg, v. 5, n. 4, p. 387-401, 2009.
10.1007/s12015-009-9098-5
WOS000273470400010.pdf
1550-8943
WOS:000273470400010
ark:/48912/001300000xhrs
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Stem Cell Reviews and Reports
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.format.none.fl_str_mv 387-401
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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dc.identifier.doi.none.fl_str_mv 10.1007/s12015-009-9098-5