Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord Units: Cord is Different from Blood
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
dARK ID: | ark:/48912/001300000xhrs |
DOI: | 10.1007/s12015-009-9098-5 |
Texto Completo: | http://dx.doi.org/10.1007/s12015-009-9098-5 http://repositorio.unifesp.br/handle/11600/31956 |
Resumo: | Mesenchymal stem cells (MSC) are multipotent cells which can be obtained from several adult and fetal tissues including human umbilical cord units. We have recently shown that umbilical cord tissue (UC) is richer in MSC than umbilical cord blood (UCB) but their origin and characteristics in blood as compared to the cord remains unknown. Here we compared, for the first time, the exonic protein-coding and intronic noncoding RNA (ncRNA) expression profiles of MSC from match-paired UC and UCB samples, harvested from the same donors, processed simultaneously and under the same culture conditions. the patterns of intronic ncRNA expression in MSC from UC and UCB paired units were highly similar, indicative of their common donor origin. the respective exonic protein-coding transcript expression profiles, however, were significantly different. Hierarchical clustering based on protein-coding expression similarities grouped MSC according to their tissue location rather than original donor. Genes related to systems development, osteogenesis and immune system were expressed at higher levels in UCB, whereas genes related to cell adhesion, morphogenesis, secretion, angiogenesis and neurogenesis were more expressed in UC cells. These molecular differences verified in tissue-specific MSC gene expression may reflect functional activities influenced by distinct niches and should be considered when developing clinical protocols involving MSC from different sources. in addition, these findings reinforce our previous suggestion on the importance of banking the whole umbilical cord unit for research or future therapeutic use. |
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Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord Units: Cord is Different from BloodHuman umbilical cordHuman umbilical cord bloodMesenchymal stem cellsComparative gene expression profileMesenchymal stem cells (MSC) are multipotent cells which can be obtained from several adult and fetal tissues including human umbilical cord units. We have recently shown that umbilical cord tissue (UC) is richer in MSC than umbilical cord blood (UCB) but their origin and characteristics in blood as compared to the cord remains unknown. Here we compared, for the first time, the exonic protein-coding and intronic noncoding RNA (ncRNA) expression profiles of MSC from match-paired UC and UCB samples, harvested from the same donors, processed simultaneously and under the same culture conditions. the patterns of intronic ncRNA expression in MSC from UC and UCB paired units were highly similar, indicative of their common donor origin. the respective exonic protein-coding transcript expression profiles, however, were significantly different. Hierarchical clustering based on protein-coding expression similarities grouped MSC according to their tissue location rather than original donor. Genes related to systems development, osteogenesis and immune system were expressed at higher levels in UCB, whereas genes related to cell adhesion, morphogenesis, secretion, angiogenesis and neurogenesis were more expressed in UC cells. These molecular differences verified in tissue-specific MSC gene expression may reflect functional activities influenced by distinct niches and should be considered when developing clinical protocols involving MSC from different sources. in addition, these findings reinforce our previous suggestion on the importance of banking the whole umbilical cord unit for research or future therapeutic use.Univ São Paulo, Dept Genet & Evolut Biol, Human Genome Res Ctr, BR-05508090 São Paulo, BrazilUniv São Paulo, Inst Quim, Dept Bioquim, BR-05508900 São Paulo, BrazilUniv Calif San Diego, Dept Pediat & Cellular & Mol Biol, UCSD Stem Cell Initiat, La Jolla, CA 92093 USAUniversidade Federal de São Paulo, Dept Neurol & Neurosurg, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Neurol & Neurosurg, BR-04023900 São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)INCT (Instituto Nacional de Ciencia e Tecnologia)SpringerUniversidade de São Paulo (USP)Univ Calif San DiegoUniversidade Federal de São Paulo (UNIFESP)Secco, MarianeMoreira, Yuri B.Zucconi, EderVieira, Natassia M.Jazedje, TatianaMuotri, Alysson R.Okamoto, Oswaldo Keith [UNIFESP]Verjovski-Almeida, SergioZatz, Mayana2016-01-24T13:58:55Z2016-01-24T13:58:55Z2009-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion387-401application/pdfhttp://dx.doi.org/10.1007/s12015-009-9098-5Stem Cell Reviews and Reports. Heidelberg: Springer Heidelberg, v. 5, n. 4, p. 387-401, 2009.10.1007/s12015-009-9098-5WOS000273470400010.pdf1550-8943http://repositorio.unifesp.br/handle/11600/31956WOS:000273470400010ark:/48912/001300000xhrsengStem Cell Reviews and Reportsinfo:eu-repo/semantics/openAccesshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-07T21:45:53Zoai:repositorio.unifesp.br/:11600/31956Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T20:43:25.544398Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord Units: Cord is Different from Blood |
title |
Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord Units: Cord is Different from Blood |
spellingShingle |
Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord Units: Cord is Different from Blood Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord Units: Cord is Different from Blood Secco, Mariane Human umbilical cord Human umbilical cord blood Mesenchymal stem cells Comparative gene expression profile Secco, Mariane Human umbilical cord Human umbilical cord blood Mesenchymal stem cells Comparative gene expression profile |
title_short |
Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord Units: Cord is Different from Blood |
title_full |
Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord Units: Cord is Different from Blood |
title_fullStr |
Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord Units: Cord is Different from Blood Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord Units: Cord is Different from Blood |
title_full_unstemmed |
Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord Units: Cord is Different from Blood Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord Units: Cord is Different from Blood |
title_sort |
Gene Expression Profile of Mesenchymal Stem Cells from Paired Umbilical Cord Units: Cord is Different from Blood |
author |
Secco, Mariane |
author_facet |
Secco, Mariane Secco, Mariane Moreira, Yuri B. Zucconi, Eder Vieira, Natassia M. Jazedje, Tatiana Muotri, Alysson R. Okamoto, Oswaldo Keith [UNIFESP] Verjovski-Almeida, Sergio Zatz, Mayana Moreira, Yuri B. Zucconi, Eder Vieira, Natassia M. Jazedje, Tatiana Muotri, Alysson R. Okamoto, Oswaldo Keith [UNIFESP] Verjovski-Almeida, Sergio Zatz, Mayana |
author_role |
author |
author2 |
Moreira, Yuri B. Zucconi, Eder Vieira, Natassia M. Jazedje, Tatiana Muotri, Alysson R. Okamoto, Oswaldo Keith [UNIFESP] Verjovski-Almeida, Sergio Zatz, Mayana |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Univ Calif San Diego Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Secco, Mariane Moreira, Yuri B. Zucconi, Eder Vieira, Natassia M. Jazedje, Tatiana Muotri, Alysson R. Okamoto, Oswaldo Keith [UNIFESP] Verjovski-Almeida, Sergio Zatz, Mayana |
dc.subject.por.fl_str_mv |
Human umbilical cord Human umbilical cord blood Mesenchymal stem cells Comparative gene expression profile |
topic |
Human umbilical cord Human umbilical cord blood Mesenchymal stem cells Comparative gene expression profile |
description |
Mesenchymal stem cells (MSC) are multipotent cells which can be obtained from several adult and fetal tissues including human umbilical cord units. We have recently shown that umbilical cord tissue (UC) is richer in MSC than umbilical cord blood (UCB) but their origin and characteristics in blood as compared to the cord remains unknown. Here we compared, for the first time, the exonic protein-coding and intronic noncoding RNA (ncRNA) expression profiles of MSC from match-paired UC and UCB samples, harvested from the same donors, processed simultaneously and under the same culture conditions. the patterns of intronic ncRNA expression in MSC from UC and UCB paired units were highly similar, indicative of their common donor origin. the respective exonic protein-coding transcript expression profiles, however, were significantly different. Hierarchical clustering based on protein-coding expression similarities grouped MSC according to their tissue location rather than original donor. Genes related to systems development, osteogenesis and immune system were expressed at higher levels in UCB, whereas genes related to cell adhesion, morphogenesis, secretion, angiogenesis and neurogenesis were more expressed in UC cells. These molecular differences verified in tissue-specific MSC gene expression may reflect functional activities influenced by distinct niches and should be considered when developing clinical protocols involving MSC from different sources. in addition, these findings reinforce our previous suggestion on the importance of banking the whole umbilical cord unit for research or future therapeutic use. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-12-01 2016-01-24T13:58:55Z 2016-01-24T13:58:55Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s12015-009-9098-5 Stem Cell Reviews and Reports. Heidelberg: Springer Heidelberg, v. 5, n. 4, p. 387-401, 2009. 10.1007/s12015-009-9098-5 WOS000273470400010.pdf 1550-8943 http://repositorio.unifesp.br/handle/11600/31956 WOS:000273470400010 |
dc.identifier.dark.fl_str_mv |
ark:/48912/001300000xhrs |
url |
http://dx.doi.org/10.1007/s12015-009-9098-5 http://repositorio.unifesp.br/handle/11600/31956 |
identifier_str_mv |
Stem Cell Reviews and Reports. Heidelberg: Springer Heidelberg, v. 5, n. 4, p. 387-401, 2009. 10.1007/s12015-009-9098-5 WOS000273470400010.pdf 1550-8943 WOS:000273470400010 ark:/48912/001300000xhrs |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Stem Cell Reviews and Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0 |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0 |
dc.format.none.fl_str_mv |
387-401 application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1822250691390865408 |
dc.identifier.doi.none.fl_str_mv |
10.1007/s12015-009-9098-5 |