Multipotent stem cells from umbilical cord: Cord is richer than blood!
Autor(a) principal: | |
---|---|
Data de Publicação: | 2008 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
dARK ID: | ark:/48912/0013000015z9q |
DOI: | 10.1634/stemcells.2007-0381 |
Texto Completo: | http://dx.doi.org/10.1634/stemcells.2007-0381 http://repositorio.unifesp.br/handle/11600/30253 |
Resumo: | The identification of mesenchymal stem cell ( MSC) sources that are easily obtainable is of utmost importance. Several studies have shown that MSCs could be isolated from umbilical cord (UC) units. However, the presence of MSCs in umbilical cord blood (UCB) is controversial. A possible explanation for the low efficiency of MSCs from UCB is the use of different culture conditions by independent studies. Here, we compared the efficiency in obtaining MSCs from unrelated paired UCB and UC samples harvested from the same donors. Samples were processed simultaneously, under the same culture conditions. Although MSCs from blood were obtained from only 1 of the 10 samples, we were able to isolate large amounts of multi-potent MSCs from all UC samples, which were able to originate different cell lineages. Since the routine procedure in UC banks has been to store the blood and discard other tissues, such as the cord and/or placenta, we believe our results are of immediate clinical value. Furthermore, the possibility of originating different cell lines from the UC of neonates born with genetic defects may provide new cellular research models for understanding human malformations and genetic disorders, as well as the possibility of testing the effects of different therapeutic drugs. |
id |
UFSP_6e438028f96cf5c8ca2dbea3d42fce56 |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br/:11600/30253 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Multipotent stem cells from umbilical cord: Cord is richer than blood!human umbilical cordhuman umbilical cord bloodmesenchymal stem cellsumbilical cord banksThe identification of mesenchymal stem cell ( MSC) sources that are easily obtainable is of utmost importance. Several studies have shown that MSCs could be isolated from umbilical cord (UC) units. However, the presence of MSCs in umbilical cord blood (UCB) is controversial. A possible explanation for the low efficiency of MSCs from UCB is the use of different culture conditions by independent studies. Here, we compared the efficiency in obtaining MSCs from unrelated paired UCB and UC samples harvested from the same donors. Samples were processed simultaneously, under the same culture conditions. Although MSCs from blood were obtained from only 1 of the 10 samples, we were able to isolate large amounts of multi-potent MSCs from all UC samples, which were able to originate different cell lineages. Since the routine procedure in UC banks has been to store the blood and discard other tissues, such as the cord and/or placenta, we believe our results are of immediate clinical value. Furthermore, the possibility of originating different cell lines from the UC of neonates born with genetic defects may provide new cellular research models for understanding human malformations and genetic disorders, as well as the possibility of testing the effects of different therapeutic drugs.Univ São Paulo, Dept Genet & Evolut Biol, Human Genome Res Ctr, BR-05508090 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Neurol & Neurosurg, São Paulo, BrazilSalk Inst Biol Studies, Genet Lab, La Jolla, CA 92037 USAUniversidade Federal de São Paulo, Dept Neurol & Neurosurg, São Paulo, BrazilWeb of ScienceAlphamed PressUniversidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)Salk Inst Biol StudiesSecco, MarianeZucconi, EderVieira, Natassia M.Fogaca, Luciana L. Q.Cerqueira, AntoniaCarvalho, Maria Denise F.Jazedje, TatianaOkamoto, Oswaldo K. [UNIFESP]Muotri, Alysson R.Zatz, Mayana2016-01-24T13:49:21Z2016-01-24T13:49:21Z2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion146-150http://dx.doi.org/10.1634/stemcells.2007-0381Stem Cells. Durham: Alphamed Press, v. 26, n. 1, p. 146-150, 2008.10.1634/stemcells.2007-03811066-5099http://repositorio.unifesp.br/handle/11600/30253WOS:000252302500017ark:/48912/0013000015z9qengStem Cellsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2016-01-24T11:49:21Zoai:repositorio.unifesp.br/:11600/30253Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T20:58:41.547271Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Multipotent stem cells from umbilical cord: Cord is richer than blood! |
title |
Multipotent stem cells from umbilical cord: Cord is richer than blood! |
spellingShingle |
Multipotent stem cells from umbilical cord: Cord is richer than blood! Multipotent stem cells from umbilical cord: Cord is richer than blood! Secco, Mariane human umbilical cord human umbilical cord blood mesenchymal stem cells umbilical cord banks Secco, Mariane human umbilical cord human umbilical cord blood mesenchymal stem cells umbilical cord banks |
title_short |
Multipotent stem cells from umbilical cord: Cord is richer than blood! |
title_full |
Multipotent stem cells from umbilical cord: Cord is richer than blood! |
title_fullStr |
Multipotent stem cells from umbilical cord: Cord is richer than blood! Multipotent stem cells from umbilical cord: Cord is richer than blood! |
title_full_unstemmed |
Multipotent stem cells from umbilical cord: Cord is richer than blood! Multipotent stem cells from umbilical cord: Cord is richer than blood! |
title_sort |
Multipotent stem cells from umbilical cord: Cord is richer than blood! |
author |
Secco, Mariane |
author_facet |
Secco, Mariane Secco, Mariane Zucconi, Eder Vieira, Natassia M. Fogaca, Luciana L. Q. Cerqueira, Antonia Carvalho, Maria Denise F. Jazedje, Tatiana Okamoto, Oswaldo K. [UNIFESP] Muotri, Alysson R. Zatz, Mayana Zucconi, Eder Vieira, Natassia M. Fogaca, Luciana L. Q. Cerqueira, Antonia Carvalho, Maria Denise F. Jazedje, Tatiana Okamoto, Oswaldo K. [UNIFESP] Muotri, Alysson R. Zatz, Mayana |
author_role |
author |
author2 |
Zucconi, Eder Vieira, Natassia M. Fogaca, Luciana L. Q. Cerqueira, Antonia Carvalho, Maria Denise F. Jazedje, Tatiana Okamoto, Oswaldo K. [UNIFESP] Muotri, Alysson R. Zatz, Mayana |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Federal de São Paulo (UNIFESP) Salk Inst Biol Studies |
dc.contributor.author.fl_str_mv |
Secco, Mariane Zucconi, Eder Vieira, Natassia M. Fogaca, Luciana L. Q. Cerqueira, Antonia Carvalho, Maria Denise F. Jazedje, Tatiana Okamoto, Oswaldo K. [UNIFESP] Muotri, Alysson R. Zatz, Mayana |
dc.subject.por.fl_str_mv |
human umbilical cord human umbilical cord blood mesenchymal stem cells umbilical cord banks |
topic |
human umbilical cord human umbilical cord blood mesenchymal stem cells umbilical cord banks |
description |
The identification of mesenchymal stem cell ( MSC) sources that are easily obtainable is of utmost importance. Several studies have shown that MSCs could be isolated from umbilical cord (UC) units. However, the presence of MSCs in umbilical cord blood (UCB) is controversial. A possible explanation for the low efficiency of MSCs from UCB is the use of different culture conditions by independent studies. Here, we compared the efficiency in obtaining MSCs from unrelated paired UCB and UC samples harvested from the same donors. Samples were processed simultaneously, under the same culture conditions. Although MSCs from blood were obtained from only 1 of the 10 samples, we were able to isolate large amounts of multi-potent MSCs from all UC samples, which were able to originate different cell lineages. Since the routine procedure in UC banks has been to store the blood and discard other tissues, such as the cord and/or placenta, we believe our results are of immediate clinical value. Furthermore, the possibility of originating different cell lines from the UC of neonates born with genetic defects may provide new cellular research models for understanding human malformations and genetic disorders, as well as the possibility of testing the effects of different therapeutic drugs. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01 2016-01-24T13:49:21Z 2016-01-24T13:49:21Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1634/stemcells.2007-0381 Stem Cells. Durham: Alphamed Press, v. 26, n. 1, p. 146-150, 2008. 10.1634/stemcells.2007-0381 1066-5099 http://repositorio.unifesp.br/handle/11600/30253 WOS:000252302500017 |
dc.identifier.dark.fl_str_mv |
ark:/48912/0013000015z9q |
url |
http://dx.doi.org/10.1634/stemcells.2007-0381 http://repositorio.unifesp.br/handle/11600/30253 |
identifier_str_mv |
Stem Cells. Durham: Alphamed Press, v. 26, n. 1, p. 146-150, 2008. 10.1634/stemcells.2007-0381 1066-5099 WOS:000252302500017 ark:/48912/0013000015z9q |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Stem Cells |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
146-150 |
dc.publisher.none.fl_str_mv |
Alphamed Press |
publisher.none.fl_str_mv |
Alphamed Press |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1822251690674356224 |
dc.identifier.doi.none.fl_str_mv |
10.1634/stemcells.2007-0381 |