Multipotent stem cells from umbilical cord: Cord is richer than blood!

Detalhes bibliográficos
Autor(a) principal: Secco, Mariane
Data de Publicação: 2008
Outros Autores: Zucconi, Eder, Vieira, Natassia M., Fogaca, Luciana L. Q., Cerqueira, Antonia, Carvalho, Maria Denise F., Jazedje, Tatiana, Okamoto, Oswaldo K. [UNIFESP], Muotri, Alysson R., Zatz, Mayana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
dARK ID: ark:/48912/0013000015z9q
DOI: 10.1634/stemcells.2007-0381
Texto Completo: http://dx.doi.org/10.1634/stemcells.2007-0381
http://repositorio.unifesp.br/handle/11600/30253
Resumo: The identification of mesenchymal stem cell ( MSC) sources that are easily obtainable is of utmost importance. Several studies have shown that MSCs could be isolated from umbilical cord (UC) units. However, the presence of MSCs in umbilical cord blood (UCB) is controversial. A possible explanation for the low efficiency of MSCs from UCB is the use of different culture conditions by independent studies. Here, we compared the efficiency in obtaining MSCs from unrelated paired UCB and UC samples harvested from the same donors. Samples were processed simultaneously, under the same culture conditions. Although MSCs from blood were obtained from only 1 of the 10 samples, we were able to isolate large amounts of multi-potent MSCs from all UC samples, which were able to originate different cell lineages. Since the routine procedure in UC banks has been to store the blood and discard other tissues, such as the cord and/or placenta, we believe our results are of immediate clinical value. Furthermore, the possibility of originating different cell lines from the UC of neonates born with genetic defects may provide new cellular research models for understanding human malformations and genetic disorders, as well as the possibility of testing the effects of different therapeutic drugs.
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spelling Multipotent stem cells from umbilical cord: Cord is richer than blood!human umbilical cordhuman umbilical cord bloodmesenchymal stem cellsumbilical cord banksThe identification of mesenchymal stem cell ( MSC) sources that are easily obtainable is of utmost importance. Several studies have shown that MSCs could be isolated from umbilical cord (UC) units. However, the presence of MSCs in umbilical cord blood (UCB) is controversial. A possible explanation for the low efficiency of MSCs from UCB is the use of different culture conditions by independent studies. Here, we compared the efficiency in obtaining MSCs from unrelated paired UCB and UC samples harvested from the same donors. Samples were processed simultaneously, under the same culture conditions. Although MSCs from blood were obtained from only 1 of the 10 samples, we were able to isolate large amounts of multi-potent MSCs from all UC samples, which were able to originate different cell lineages. Since the routine procedure in UC banks has been to store the blood and discard other tissues, such as the cord and/or placenta, we believe our results are of immediate clinical value. Furthermore, the possibility of originating different cell lines from the UC of neonates born with genetic defects may provide new cellular research models for understanding human malformations and genetic disorders, as well as the possibility of testing the effects of different therapeutic drugs.Univ São Paulo, Dept Genet & Evolut Biol, Human Genome Res Ctr, BR-05508090 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Neurol & Neurosurg, São Paulo, BrazilSalk Inst Biol Studies, Genet Lab, La Jolla, CA 92037 USAUniversidade Federal de São Paulo, Dept Neurol & Neurosurg, São Paulo, BrazilWeb of ScienceAlphamed PressUniversidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)Salk Inst Biol StudiesSecco, MarianeZucconi, EderVieira, Natassia M.Fogaca, Luciana L. Q.Cerqueira, AntoniaCarvalho, Maria Denise F.Jazedje, TatianaOkamoto, Oswaldo K. [UNIFESP]Muotri, Alysson R.Zatz, Mayana2016-01-24T13:49:21Z2016-01-24T13:49:21Z2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion146-150http://dx.doi.org/10.1634/stemcells.2007-0381Stem Cells. Durham: Alphamed Press, v. 26, n. 1, p. 146-150, 2008.10.1634/stemcells.2007-03811066-5099http://repositorio.unifesp.br/handle/11600/30253WOS:000252302500017ark:/48912/0013000015z9qengStem Cellsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2016-01-24T11:49:21Zoai:repositorio.unifesp.br/:11600/30253Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T20:58:41.547271Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Multipotent stem cells from umbilical cord: Cord is richer than blood!
title Multipotent stem cells from umbilical cord: Cord is richer than blood!
spellingShingle Multipotent stem cells from umbilical cord: Cord is richer than blood!
Multipotent stem cells from umbilical cord: Cord is richer than blood!
Secco, Mariane
human umbilical cord
human umbilical cord blood
mesenchymal stem cells
umbilical cord banks
Secco, Mariane
human umbilical cord
human umbilical cord blood
mesenchymal stem cells
umbilical cord banks
title_short Multipotent stem cells from umbilical cord: Cord is richer than blood!
title_full Multipotent stem cells from umbilical cord: Cord is richer than blood!
title_fullStr Multipotent stem cells from umbilical cord: Cord is richer than blood!
Multipotent stem cells from umbilical cord: Cord is richer than blood!
title_full_unstemmed Multipotent stem cells from umbilical cord: Cord is richer than blood!
Multipotent stem cells from umbilical cord: Cord is richer than blood!
title_sort Multipotent stem cells from umbilical cord: Cord is richer than blood!
author Secco, Mariane
author_facet Secco, Mariane
Secco, Mariane
Zucconi, Eder
Vieira, Natassia M.
Fogaca, Luciana L. Q.
Cerqueira, Antonia
Carvalho, Maria Denise F.
Jazedje, Tatiana
Okamoto, Oswaldo K. [UNIFESP]
Muotri, Alysson R.
Zatz, Mayana
Zucconi, Eder
Vieira, Natassia M.
Fogaca, Luciana L. Q.
Cerqueira, Antonia
Carvalho, Maria Denise F.
Jazedje, Tatiana
Okamoto, Oswaldo K. [UNIFESP]
Muotri, Alysson R.
Zatz, Mayana
author_role author
author2 Zucconi, Eder
Vieira, Natassia M.
Fogaca, Luciana L. Q.
Cerqueira, Antonia
Carvalho, Maria Denise F.
Jazedje, Tatiana
Okamoto, Oswaldo K. [UNIFESP]
Muotri, Alysson R.
Zatz, Mayana
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Salk Inst Biol Studies
dc.contributor.author.fl_str_mv Secco, Mariane
Zucconi, Eder
Vieira, Natassia M.
Fogaca, Luciana L. Q.
Cerqueira, Antonia
Carvalho, Maria Denise F.
Jazedje, Tatiana
Okamoto, Oswaldo K. [UNIFESP]
Muotri, Alysson R.
Zatz, Mayana
dc.subject.por.fl_str_mv human umbilical cord
human umbilical cord blood
mesenchymal stem cells
umbilical cord banks
topic human umbilical cord
human umbilical cord blood
mesenchymal stem cells
umbilical cord banks
description The identification of mesenchymal stem cell ( MSC) sources that are easily obtainable is of utmost importance. Several studies have shown that MSCs could be isolated from umbilical cord (UC) units. However, the presence of MSCs in umbilical cord blood (UCB) is controversial. A possible explanation for the low efficiency of MSCs from UCB is the use of different culture conditions by independent studies. Here, we compared the efficiency in obtaining MSCs from unrelated paired UCB and UC samples harvested from the same donors. Samples were processed simultaneously, under the same culture conditions. Although MSCs from blood were obtained from only 1 of the 10 samples, we were able to isolate large amounts of multi-potent MSCs from all UC samples, which were able to originate different cell lineages. Since the routine procedure in UC banks has been to store the blood and discard other tissues, such as the cord and/or placenta, we believe our results are of immediate clinical value. Furthermore, the possibility of originating different cell lines from the UC of neonates born with genetic defects may provide new cellular research models for understanding human malformations and genetic disorders, as well as the possibility of testing the effects of different therapeutic drugs.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
2016-01-24T13:49:21Z
2016-01-24T13:49:21Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1634/stemcells.2007-0381
Stem Cells. Durham: Alphamed Press, v. 26, n. 1, p. 146-150, 2008.
10.1634/stemcells.2007-0381
1066-5099
http://repositorio.unifesp.br/handle/11600/30253
WOS:000252302500017
dc.identifier.dark.fl_str_mv ark:/48912/0013000015z9q
url http://dx.doi.org/10.1634/stemcells.2007-0381
http://repositorio.unifesp.br/handle/11600/30253
identifier_str_mv Stem Cells. Durham: Alphamed Press, v. 26, n. 1, p. 146-150, 2008.
10.1634/stemcells.2007-0381
1066-5099
WOS:000252302500017
ark:/48912/0013000015z9q
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Stem Cells
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 146-150
dc.publisher.none.fl_str_mv Alphamed Press
publisher.none.fl_str_mv Alphamed Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1822251690674356224
dc.identifier.doi.none.fl_str_mv 10.1634/stemcells.2007-0381