Validation of Housekeeping Genes in the Brains of Rats Submitted to Chronic Intermittent Hypoxia, a Sleep Apnea Model
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/38326 http://dx.doi.org/10.1371/journal.pone.0109902 |
Resumo: | Obstructive sleep apnea (OSA) is a syndrome characterized by intermittent nocturnal hypoxia, sleep fragmentation, hypercapnia and respiratory effort, and it has been associated with several complications, such as diabetes, hypertension and obesity. Quantitative real-time PCR has been performed in previous OSA-related studies; however, these studies were not validated using proper reference genes. We have examined the effects of chronic intermittent hypoxia (CIH), which is an experimental model mainly of cardiovascular consequences of OSA, on reference genes, including beta-actin, beta-2-microglobulin, glyceraldehyde-3-phosphate dehydrogenase, hypoxanthine guanine phosphoribosyl transferase and eukaryotic 18S rRNA, in different areas of the brain. All stability analyses were performed using the geNorm, Normfinder and BestKeeper software programs. With exception of the 18S rRNA, all of the evaluated genes were shown to be stable following CIH exposure. However, gene stability rankings were dependent on the area of the brain that was analyzed and varied according to the software that was used. This study demonstrated that CIH affects various brain structures differently. With the exception of the 18S rRNA, all of the tested genes are suitable for use as housekeeping genes in expression analyses. |
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Julian, Guilherme Silva [UNIFESP]Oliveira, Renato Watanabe de [UNIFESP]Perry, Juliana Cini [UNIFESP]Tufik, Sergio [UNIFESP]Chagas, Jair Ribeiro [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2016-01-24T14:38:00Z2016-01-24T14:38:00Z2014-10-07Plos One. San Francisco: Public Library Science, v. 9, n. 10, 8 p., 2014.1932-6203http://repositorio.unifesp.br/handle/11600/38326http://dx.doi.org/10.1371/journal.pone.0109902WOS000342798000098.pdf10.1371/journal.pone.0109902WOS:000342798000098Obstructive sleep apnea (OSA) is a syndrome characterized by intermittent nocturnal hypoxia, sleep fragmentation, hypercapnia and respiratory effort, and it has been associated with several complications, such as diabetes, hypertension and obesity. Quantitative real-time PCR has been performed in previous OSA-related studies; however, these studies were not validated using proper reference genes. We have examined the effects of chronic intermittent hypoxia (CIH), which is an experimental model mainly of cardiovascular consequences of OSA, on reference genes, including beta-actin, beta-2-microglobulin, glyceraldehyde-3-phosphate dehydrogenase, hypoxanthine guanine phosphoribosyl transferase and eukaryotic 18S rRNA, in different areas of the brain. All stability analyses were performed using the geNorm, Normfinder and BestKeeper software programs. With exception of the 18S rRNA, all of the evaluated genes were shown to be stable following CIH exposure. However, gene stability rankings were dependent on the area of the brain that was analyzed and varied according to the software that was used. This study demonstrated that CIH affects various brain structures differently. With the exception of the 18S rRNA, all of the tested genes are suitable for use as housekeeping genes in expression analyses.Associacao Fundo de Incentivo a Pesquisa (AFIP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal de São Paulo, Dept Psicobiol, UNIFESP, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biociencias, UNIFESP Baixada Santista, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Psicobiol, UNIFESP, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biociencias, UNIFESP Baixada Santista, São Paulo, BrazilFAPESP: 2011/15060-4FAPESP: 2011/16011-6CNPq: 558924/2008-5Web of Science8engPublic Library SciencePlos OneValidation of Housekeeping Genes in the Brains of Rats Submitted to Chronic Intermittent Hypoxia, a Sleep Apnea Modelinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000342798000098.pdfapplication/pdf872665${dspace.ui.url}/bitstream/11600/38326/1/WOS000342798000098.pdf3b4c76b858eca22bee321e2a54c8616dMD51open accessTEXTWOS000342798000098.pdf.txtWOS000342798000098.pdf.txtExtracted texttext/plain42002${dspace.ui.url}/bitstream/11600/38326/2/WOS000342798000098.pdf.txta138ec700732fb9a60c3e8ee408327c7MD52open access11600/383262021-10-05 21:28:54.418open accessoai:repositorio.unifesp.br:11600/38326Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652021-10-06T00:28:54Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Validation of Housekeeping Genes in the Brains of Rats Submitted to Chronic Intermittent Hypoxia, a Sleep Apnea Model |
title |
Validation of Housekeeping Genes in the Brains of Rats Submitted to Chronic Intermittent Hypoxia, a Sleep Apnea Model |
spellingShingle |
Validation of Housekeeping Genes in the Brains of Rats Submitted to Chronic Intermittent Hypoxia, a Sleep Apnea Model Julian, Guilherme Silva [UNIFESP] |
title_short |
Validation of Housekeeping Genes in the Brains of Rats Submitted to Chronic Intermittent Hypoxia, a Sleep Apnea Model |
title_full |
Validation of Housekeeping Genes in the Brains of Rats Submitted to Chronic Intermittent Hypoxia, a Sleep Apnea Model |
title_fullStr |
Validation of Housekeeping Genes in the Brains of Rats Submitted to Chronic Intermittent Hypoxia, a Sleep Apnea Model |
title_full_unstemmed |
Validation of Housekeeping Genes in the Brains of Rats Submitted to Chronic Intermittent Hypoxia, a Sleep Apnea Model |
title_sort |
Validation of Housekeeping Genes in the Brains of Rats Submitted to Chronic Intermittent Hypoxia, a Sleep Apnea Model |
author |
Julian, Guilherme Silva [UNIFESP] |
author_facet |
Julian, Guilherme Silva [UNIFESP] Oliveira, Renato Watanabe de [UNIFESP] Perry, Juliana Cini [UNIFESP] Tufik, Sergio [UNIFESP] Chagas, Jair Ribeiro [UNIFESP] |
author_role |
author |
author2 |
Oliveira, Renato Watanabe de [UNIFESP] Perry, Juliana Cini [UNIFESP] Tufik, Sergio [UNIFESP] Chagas, Jair Ribeiro [UNIFESP] |
author2_role |
author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Julian, Guilherme Silva [UNIFESP] Oliveira, Renato Watanabe de [UNIFESP] Perry, Juliana Cini [UNIFESP] Tufik, Sergio [UNIFESP] Chagas, Jair Ribeiro [UNIFESP] |
description |
Obstructive sleep apnea (OSA) is a syndrome characterized by intermittent nocturnal hypoxia, sleep fragmentation, hypercapnia and respiratory effort, and it has been associated with several complications, such as diabetes, hypertension and obesity. Quantitative real-time PCR has been performed in previous OSA-related studies; however, these studies were not validated using proper reference genes. We have examined the effects of chronic intermittent hypoxia (CIH), which is an experimental model mainly of cardiovascular consequences of OSA, on reference genes, including beta-actin, beta-2-microglobulin, glyceraldehyde-3-phosphate dehydrogenase, hypoxanthine guanine phosphoribosyl transferase and eukaryotic 18S rRNA, in different areas of the brain. All stability analyses were performed using the geNorm, Normfinder and BestKeeper software programs. With exception of the 18S rRNA, all of the evaluated genes were shown to be stable following CIH exposure. However, gene stability rankings were dependent on the area of the brain that was analyzed and varied according to the software that was used. This study demonstrated that CIH affects various brain structures differently. With the exception of the 18S rRNA, all of the tested genes are suitable for use as housekeeping genes in expression analyses. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-10-07 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:38:00Z |
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2016-01-24T14:38:00Z |
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info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Plos One. San Francisco: Public Library Science, v. 9, n. 10, 8 p., 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/38326 http://dx.doi.org/10.1371/journal.pone.0109902 |
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1932-6203 |
dc.identifier.file.none.fl_str_mv |
WOS000342798000098.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0109902 |
dc.identifier.wos.none.fl_str_mv |
WOS:000342798000098 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 9, n. 10, 8 p., 2014. 1932-6203 WOS000342798000098.pdf 10.1371/journal.pone.0109902 WOS:000342798000098 |
url |
http://repositorio.unifesp.br/handle/11600/38326 http://dx.doi.org/10.1371/journal.pone.0109902 |
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Public Library Science |
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