Recruitment of galectin-3 during cell invasion and intracellular trafficking of Trypanosoma cruzi extracellular amastigotes
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/37340 http://dx.doi.org/10.1093/glycob/cwt097 |
Resumo: | The invasion of host cells by the intracellular protozoan Trypanosoma cruzi requires interactions with host cell molecules, and the replication of the parasite requires escape from a parasitophorous vacuole into the host cell cytosol. Galectin-3, a member of beta-galactosidase-binding lectin family, has numerous extracellular and intracellular functions. in this study, we investigated the role of galectin-3 during the invasion and intracellular trafficking of T. cruzi extracellular amastigotes (EAs). Endogenous galectin-3 from mouse peritoneal macrophages accumulated around the pathogen during cell invasion by EAs. in addition, galectin-3 accumulated around parasites after their escape from the parasitophorous vacuole. Thus, galectin-3 behaved as a novel marker of phagolysosome lysis during the infection of host cells by T. cruzi. |
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Machado, Fabricio CastroCruz, LilianSilva, Aline Alves daCruz, Mario Costa [UNIFESP]Mortara, Renato Arruda [UNIFESP]Roque-Barreira, Maria CristinaSilva, Claudio Vieira daUniversidade Federal de Uberlândia (UFU)Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)2016-01-24T14:35:10Z2016-01-24T14:35:10Z2014-02-01Glycobiology. Cary: Oxford Univ Press Inc, v. 24, n. 2, p. 179-184, 2014.0959-6658http://repositorio.unifesp.br/handle/11600/37340http://dx.doi.org/10.1093/glycob/cwt09710.1093/glycob/cwt097WOS:000330839300007The invasion of host cells by the intracellular protozoan Trypanosoma cruzi requires interactions with host cell molecules, and the replication of the parasite requires escape from a parasitophorous vacuole into the host cell cytosol. Galectin-3, a member of beta-galactosidase-binding lectin family, has numerous extracellular and intracellular functions. in this study, we investigated the role of galectin-3 during the invasion and intracellular trafficking of T. cruzi extracellular amastigotes (EAs). Endogenous galectin-3 from mouse peritoneal macrophages accumulated around the pathogen during cell invasion by EAs. in addition, galectin-3 accumulated around parasites after their escape from the parasitophorous vacuole. Thus, galectin-3 behaved as a novel marker of phagolysosome lysis during the infection of host cells by T. cruzi.Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Uberlandia, Lab Tripanosomatideos, Disciplina Imunol, Inst Ciencias Biomed, BR-38400902 Uberlandia, MG, BrazilUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilUniv São Paulo, Dept Biol Celular & Mol & Bioagentes Patogen, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilFAPEMIG: PIBIC B 004/2009FAPEMIG: APQ-00621-11FAPEMIG: APQ-00305-12CAPES: 23038.005295/2011-40Web of Science179-184engOxford Univ Press IncGlycobiologyhttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.htmlinfo:eu-repo/semantics/openAccessgalectin-3intracellular trafficphagolysosome lysisTrypanosoma cruziRecruitment of galectin-3 during cell invasion and intracellular trafficking of Trypanosoma cruzi extracellular amastigotesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPMicrobiologia, Imunologia e Parasitologia11600/373402023-01-23 21:38:00.95metadata only accessoai:repositorio.unifesp.br:11600/37340Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-01-24T00:38Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Recruitment of galectin-3 during cell invasion and intracellular trafficking of Trypanosoma cruzi extracellular amastigotes |
title |
Recruitment of galectin-3 during cell invasion and intracellular trafficking of Trypanosoma cruzi extracellular amastigotes |
spellingShingle |
Recruitment of galectin-3 during cell invasion and intracellular trafficking of Trypanosoma cruzi extracellular amastigotes Machado, Fabricio Castro galectin-3 intracellular traffic phagolysosome lysis Trypanosoma cruzi |
title_short |
Recruitment of galectin-3 during cell invasion and intracellular trafficking of Trypanosoma cruzi extracellular amastigotes |
title_full |
Recruitment of galectin-3 during cell invasion and intracellular trafficking of Trypanosoma cruzi extracellular amastigotes |
title_fullStr |
Recruitment of galectin-3 during cell invasion and intracellular trafficking of Trypanosoma cruzi extracellular amastigotes |
title_full_unstemmed |
Recruitment of galectin-3 during cell invasion and intracellular trafficking of Trypanosoma cruzi extracellular amastigotes |
title_sort |
Recruitment of galectin-3 during cell invasion and intracellular trafficking of Trypanosoma cruzi extracellular amastigotes |
author |
Machado, Fabricio Castro |
author_facet |
Machado, Fabricio Castro Cruz, Lilian Silva, Aline Alves da Cruz, Mario Costa [UNIFESP] Mortara, Renato Arruda [UNIFESP] Roque-Barreira, Maria Cristina Silva, Claudio Vieira da |
author_role |
author |
author2 |
Cruz, Lilian Silva, Aline Alves da Cruz, Mario Costa [UNIFESP] Mortara, Renato Arruda [UNIFESP] Roque-Barreira, Maria Cristina Silva, Claudio Vieira da |
author2_role |
author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de Uberlândia (UFU) Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Machado, Fabricio Castro Cruz, Lilian Silva, Aline Alves da Cruz, Mario Costa [UNIFESP] Mortara, Renato Arruda [UNIFESP] Roque-Barreira, Maria Cristina Silva, Claudio Vieira da |
dc.subject.eng.fl_str_mv |
galectin-3 intracellular traffic phagolysosome lysis Trypanosoma cruzi |
topic |
galectin-3 intracellular traffic phagolysosome lysis Trypanosoma cruzi |
description |
The invasion of host cells by the intracellular protozoan Trypanosoma cruzi requires interactions with host cell molecules, and the replication of the parasite requires escape from a parasitophorous vacuole into the host cell cytosol. Galectin-3, a member of beta-galactosidase-binding lectin family, has numerous extracellular and intracellular functions. in this study, we investigated the role of galectin-3 during the invasion and intracellular trafficking of T. cruzi extracellular amastigotes (EAs). Endogenous galectin-3 from mouse peritoneal macrophages accumulated around the pathogen during cell invasion by EAs. in addition, galectin-3 accumulated around parasites after their escape from the parasitophorous vacuole. Thus, galectin-3 behaved as a novel marker of phagolysosome lysis during the infection of host cells by T. cruzi. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-02-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:35:10Z |
dc.date.available.fl_str_mv |
2016-01-24T14:35:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Glycobiology. Cary: Oxford Univ Press Inc, v. 24, n. 2, p. 179-184, 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/37340 http://dx.doi.org/10.1093/glycob/cwt097 |
dc.identifier.issn.none.fl_str_mv |
0959-6658 |
dc.identifier.doi.none.fl_str_mv |
10.1093/glycob/cwt097 |
dc.identifier.wos.none.fl_str_mv |
WOS:000330839300007 |
identifier_str_mv |
Glycobiology. Cary: Oxford Univ Press Inc, v. 24, n. 2, p. 179-184, 2014. 0959-6658 10.1093/glycob/cwt097 WOS:000330839300007 |
url |
http://repositorio.unifesp.br/handle/11600/37340 http://dx.doi.org/10.1093/glycob/cwt097 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Glycobiology |
dc.rights.driver.fl_str_mv |
http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
179-184 |
dc.publisher.none.fl_str_mv |
Oxford Univ Press Inc |
publisher.none.fl_str_mv |
Oxford Univ Press Inc |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764274708250624 |