Recruitment of galectin-3 during cell invasion and intracellular trafficking of Trypanosoma cruzi extracellular amastigotes

Detalhes bibliográficos
Autor(a) principal: Machado, Fabricio Castro
Data de Publicação: 2014
Outros Autores: Cruz, Lilian, Silva, Aline Alves da, Cruz, Mario Costa [UNIFESP], Mortara, Renato Arruda [UNIFESP], Roque-Barreira, Maria Cristina, Silva, Claudio Vieira da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://repositorio.unifesp.br/handle/11600/37340
http://dx.doi.org/10.1093/glycob/cwt097
Resumo: The invasion of host cells by the intracellular protozoan Trypanosoma cruzi requires interactions with host cell molecules, and the replication of the parasite requires escape from a parasitophorous vacuole into the host cell cytosol. Galectin-3, a member of beta-galactosidase-binding lectin family, has numerous extracellular and intracellular functions. in this study, we investigated the role of galectin-3 during the invasion and intracellular trafficking of T. cruzi extracellular amastigotes (EAs). Endogenous galectin-3 from mouse peritoneal macrophages accumulated around the pathogen during cell invasion by EAs. in addition, galectin-3 accumulated around parasites after their escape from the parasitophorous vacuole. Thus, galectin-3 behaved as a novel marker of phagolysosome lysis during the infection of host cells by T. cruzi.
id UFSP_5cf7fd49ded9595412bf096ff14dfe27
oai_identifier_str oai:repositorio.unifesp.br:11600/37340
network_acronym_str UFSP
network_name_str Repositório Institucional da UNIFESP
repository_id_str 3465
spelling Machado, Fabricio CastroCruz, LilianSilva, Aline Alves daCruz, Mario Costa [UNIFESP]Mortara, Renato Arruda [UNIFESP]Roque-Barreira, Maria CristinaSilva, Claudio Vieira daUniversidade Federal de Uberlândia (UFU)Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)2016-01-24T14:35:10Z2016-01-24T14:35:10Z2014-02-01Glycobiology. Cary: Oxford Univ Press Inc, v. 24, n. 2, p. 179-184, 2014.0959-6658http://repositorio.unifesp.br/handle/11600/37340http://dx.doi.org/10.1093/glycob/cwt09710.1093/glycob/cwt097WOS:000330839300007The invasion of host cells by the intracellular protozoan Trypanosoma cruzi requires interactions with host cell molecules, and the replication of the parasite requires escape from a parasitophorous vacuole into the host cell cytosol. Galectin-3, a member of beta-galactosidase-binding lectin family, has numerous extracellular and intracellular functions. in this study, we investigated the role of galectin-3 during the invasion and intracellular trafficking of T. cruzi extracellular amastigotes (EAs). Endogenous galectin-3 from mouse peritoneal macrophages accumulated around the pathogen during cell invasion by EAs. in addition, galectin-3 accumulated around parasites after their escape from the parasitophorous vacuole. Thus, galectin-3 behaved as a novel marker of phagolysosome lysis during the infection of host cells by T. cruzi.Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Uberlandia, Lab Tripanosomatideos, Disciplina Imunol, Inst Ciencias Biomed, BR-38400902 Uberlandia, MG, BrazilUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilUniv São Paulo, Dept Biol Celular & Mol & Bioagentes Patogen, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilFAPEMIG: PIBIC B 004/2009FAPEMIG: APQ-00621-11FAPEMIG: APQ-00305-12CAPES: 23038.005295/2011-40Web of Science179-184engOxford Univ Press IncGlycobiologyhttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.htmlinfo:eu-repo/semantics/openAccessgalectin-3intracellular trafficphagolysosome lysisTrypanosoma cruziRecruitment of galectin-3 during cell invasion and intracellular trafficking of Trypanosoma cruzi extracellular amastigotesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPMicrobiologia, Imunologia e Parasitologia11600/373402023-01-23 21:38:00.95metadata only accessoai:repositorio.unifesp.br:11600/37340Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:43:59.722458Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.en.fl_str_mv Recruitment of galectin-3 during cell invasion and intracellular trafficking of Trypanosoma cruzi extracellular amastigotes
title Recruitment of galectin-3 during cell invasion and intracellular trafficking of Trypanosoma cruzi extracellular amastigotes
spellingShingle Recruitment of galectin-3 during cell invasion and intracellular trafficking of Trypanosoma cruzi extracellular amastigotes
Machado, Fabricio Castro
galectin-3
intracellular traffic
phagolysosome lysis
Trypanosoma cruzi
title_short Recruitment of galectin-3 during cell invasion and intracellular trafficking of Trypanosoma cruzi extracellular amastigotes
title_full Recruitment of galectin-3 during cell invasion and intracellular trafficking of Trypanosoma cruzi extracellular amastigotes
title_fullStr Recruitment of galectin-3 during cell invasion and intracellular trafficking of Trypanosoma cruzi extracellular amastigotes
title_full_unstemmed Recruitment of galectin-3 during cell invasion and intracellular trafficking of Trypanosoma cruzi extracellular amastigotes
title_sort Recruitment of galectin-3 during cell invasion and intracellular trafficking of Trypanosoma cruzi extracellular amastigotes
author Machado, Fabricio Castro
author_facet Machado, Fabricio Castro
Cruz, Lilian
Silva, Aline Alves da
Cruz, Mario Costa [UNIFESP]
Mortara, Renato Arruda [UNIFESP]
Roque-Barreira, Maria Cristina
Silva, Claudio Vieira da
author_role author
author2 Cruz, Lilian
Silva, Aline Alves da
Cruz, Mario Costa [UNIFESP]
Mortara, Renato Arruda [UNIFESP]
Roque-Barreira, Maria Cristina
Silva, Claudio Vieira da
author2_role author
author
author
author
author
author
dc.contributor.institution.none.fl_str_mv Universidade Federal de Uberlândia (UFU)
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Machado, Fabricio Castro
Cruz, Lilian
Silva, Aline Alves da
Cruz, Mario Costa [UNIFESP]
Mortara, Renato Arruda [UNIFESP]
Roque-Barreira, Maria Cristina
Silva, Claudio Vieira da
dc.subject.eng.fl_str_mv galectin-3
intracellular traffic
phagolysosome lysis
Trypanosoma cruzi
topic galectin-3
intracellular traffic
phagolysosome lysis
Trypanosoma cruzi
description The invasion of host cells by the intracellular protozoan Trypanosoma cruzi requires interactions with host cell molecules, and the replication of the parasite requires escape from a parasitophorous vacuole into the host cell cytosol. Galectin-3, a member of beta-galactosidase-binding lectin family, has numerous extracellular and intracellular functions. in this study, we investigated the role of galectin-3 during the invasion and intracellular trafficking of T. cruzi extracellular amastigotes (EAs). Endogenous galectin-3 from mouse peritoneal macrophages accumulated around the pathogen during cell invasion by EAs. in addition, galectin-3 accumulated around parasites after their escape from the parasitophorous vacuole. Thus, galectin-3 behaved as a novel marker of phagolysosome lysis during the infection of host cells by T. cruzi.
publishDate 2014
dc.date.issued.fl_str_mv 2014-02-01
dc.date.accessioned.fl_str_mv 2016-01-24T14:35:10Z
dc.date.available.fl_str_mv 2016-01-24T14:35:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv Glycobiology. Cary: Oxford Univ Press Inc, v. 24, n. 2, p. 179-184, 2014.
dc.identifier.uri.fl_str_mv http://repositorio.unifesp.br/handle/11600/37340
http://dx.doi.org/10.1093/glycob/cwt097
dc.identifier.issn.none.fl_str_mv 0959-6658
dc.identifier.doi.none.fl_str_mv 10.1093/glycob/cwt097
dc.identifier.wos.none.fl_str_mv WOS:000330839300007
identifier_str_mv Glycobiology. Cary: Oxford Univ Press Inc, v. 24, n. 2, p. 179-184, 2014.
0959-6658
10.1093/glycob/cwt097
WOS:000330839300007
url http://repositorio.unifesp.br/handle/11600/37340
http://dx.doi.org/10.1093/glycob/cwt097
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv Glycobiology
dc.rights.driver.fl_str_mv http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 179-184
dc.publisher.none.fl_str_mv Oxford Univ Press Inc
publisher.none.fl_str_mv Oxford Univ Press Inc
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv
_version_ 1783460332959694848

Registros relacionados