High prevalence of diarrheagenic Escherichia coli carrying toxin-encoding genes isolated from children and adults in southeastern Brazil

Detalhes bibliográficos
Autor(a) principal: Spano, Liliana Cruz
Data de Publicação: 2017
Outros Autores: da Cunha, Keyla Fonseca, Monfardini, Mariane Vedovatti [UNIFESP], Bergamaschi Fonseca, Rita de Cassia, Affonso Scaletsky, Isabel Christina [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: https://repositorio.unifesp.br/handle/11600/53989
http://dx.doi.org/10.1186/s12879-017-2872-0
Resumo: Background: Diarrheagenic Escherichia coli (DEC) are important bacterial causes of childhood diarrhea in Brazil, but its impact in adults is unknown. This study aimed at investigating DEC among children and adults living in endemic areas. Methods: A total of 327 stools specimens were collected from children (n = 141) and adults (n = 186) with diarrhea attending health centers. Diarrheagenic E. coli (DEC) were identified by their virulence genes (multiplex polymerase chain reaction) and HEp-2 cell adherence patterns. Results: DEC were detected in 56 (40%) children and 74 (39%) adults
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spelling Spano, Liliana Cruzda Cunha, Keyla FonsecaMonfardini, Mariane Vedovatti [UNIFESP]Bergamaschi Fonseca, Rita de CassiaAffonso Scaletsky, Isabel Christina [UNIFESP]2020-07-02T18:52:16Z2020-07-02T18:52:16Z2017Bmc Infectious Diseases. London, v. 17, p. -, 2017.1471-2334https://repositorio.unifesp.br/handle/11600/53989http://dx.doi.org/10.1186/s12879-017-2872-0WOS000418160300001.pdf10.1186/s12879-017-2872-0WOS:000418160300001Background: Diarrheagenic Escherichia coli (DEC) are important bacterial causes of childhood diarrhea in Brazil, but its impact in adults is unknown. This study aimed at investigating DEC among children and adults living in endemic areas. Methods: A total of 327 stools specimens were collected from children (n = 141) and adults (n = 186) with diarrhea attending health centers. Diarrheagenic E. coli (DEC) were identified by their virulence genes (multiplex polymerase chain reaction) and HEp-2 cell adherence patterns. Results: DEC were detected in 56 (40%) children and 74 (39%) adultsenteroaggregative E. coli (EAEC) (23%) was the most prevalent pathotype, followed by diffusely adherent E. coli (DAEC) (13%), and occurred at similar frequencies in both diarrheal groups. Atypical enteropathogenic E. coli (aEPEC) strains were recovered more frequently from children (6%) than from adults (1%). Twenty-six percent of the EAEC were classified as typical EAEC possessing aggR gene, and carried the aap gene. EAEC strains carrying aggR-aap-aatA genes were significantly more frequent among children than adults (p < 0.05). DAEC strains possessing Afa/Dr. genes were detected from children (10%) and adults (6%). EAEC and DAEC strains harboring genes for the EAST1 (astA), Pet, Pic, and Sat toxins were common in both diarrheal groups. The astA and the porcine AE/associated adhesin (paa) genes were found in most of aEPEC strains. High levels of resistance to antimicrobial drugs were found among DAEC and aEPEC isolates. Conclusion: The results show a high proportion of EAEC and DAEC carrying toxin-encoding genes among adults with diarrhea.Foundation for Science and Technology of Victoria Municipality (FACITEC) of Espirito Santo State, BrazilUniv Fed Espirito Santo, Ctr Ciencias Saude, Dept Patol, Lab Virol & Gastrenterite Infecciosa, Ave Marechal Campos 1468, BR-29043900 Vitoria, ES, BrazilUniv Fed Espirito Santo, Nucleo Doencas Infecciosas, Dept Med Social, Ctr Ciencias Saude, Vitoria, ES, BrazilUniv Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Vitoria, ES, BrazilLab Cent Municipal, Vitoria, ES, BrazilUniv Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Vitoria, ES, BrazilWeb of Science-engBiomed Central LtdBmc Infectious DiseasesDiarrheagenic E. coliChildrenAdultsHigh prevalence of diarrheagenic Escherichia coli carrying toxin-encoding genes isolated from children and adults in southeastern Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLondon17info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000418160300001.pdfapplication/pdf635641${dspace.ui.url}/bitstream/11600/53989/1/WOS000418160300001.pdfecf567c8f18d6c2fd1156dcdb5603f00MD51open accessTEXTWOS000418160300001.pdf.txtWOS000418160300001.pdf.txtExtracted texttext/plain39087${dspace.ui.url}/bitstream/11600/53989/2/WOS000418160300001.pdf.txtf3d8118e318eef398e705dc30df928a6MD52open accessTHUMBNAILWOS000418160300001.pdf.jpgWOS000418160300001.pdf.jpgIM Thumbnailimage/jpeg6878${dspace.ui.url}/bitstream/11600/53989/4/WOS000418160300001.pdf.jpg5e8b05cbf93c069fbcb60f8a03b950e1MD54open access11600/539892022-08-01 08:27:44.569open accessoai:repositorio.unifesp.br:11600/53989Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:22:18.576636Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.en.fl_str_mv High prevalence of diarrheagenic Escherichia coli carrying toxin-encoding genes isolated from children and adults in southeastern Brazil
title High prevalence of diarrheagenic Escherichia coli carrying toxin-encoding genes isolated from children and adults in southeastern Brazil
spellingShingle High prevalence of diarrheagenic Escherichia coli carrying toxin-encoding genes isolated from children and adults in southeastern Brazil
Spano, Liliana Cruz
Diarrheagenic E. coli
Children
Adults
title_short High prevalence of diarrheagenic Escherichia coli carrying toxin-encoding genes isolated from children and adults in southeastern Brazil
title_full High prevalence of diarrheagenic Escherichia coli carrying toxin-encoding genes isolated from children and adults in southeastern Brazil
title_fullStr High prevalence of diarrheagenic Escherichia coli carrying toxin-encoding genes isolated from children and adults in southeastern Brazil
title_full_unstemmed High prevalence of diarrheagenic Escherichia coli carrying toxin-encoding genes isolated from children and adults in southeastern Brazil
title_sort High prevalence of diarrheagenic Escherichia coli carrying toxin-encoding genes isolated from children and adults in southeastern Brazil
author Spano, Liliana Cruz
author_facet Spano, Liliana Cruz
da Cunha, Keyla Fonseca
Monfardini, Mariane Vedovatti [UNIFESP]
Bergamaschi Fonseca, Rita de Cassia
Affonso Scaletsky, Isabel Christina [UNIFESP]
author_role author
author2 da Cunha, Keyla Fonseca
Monfardini, Mariane Vedovatti [UNIFESP]
Bergamaschi Fonseca, Rita de Cassia
Affonso Scaletsky, Isabel Christina [UNIFESP]
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Spano, Liliana Cruz
da Cunha, Keyla Fonseca
Monfardini, Mariane Vedovatti [UNIFESP]
Bergamaschi Fonseca, Rita de Cassia
Affonso Scaletsky, Isabel Christina [UNIFESP]
dc.subject.eng.fl_str_mv Diarrheagenic E. coli
Children
Adults
topic Diarrheagenic E. coli
Children
Adults
description Background: Diarrheagenic Escherichia coli (DEC) are important bacterial causes of childhood diarrhea in Brazil, but its impact in adults is unknown. This study aimed at investigating DEC among children and adults living in endemic areas. Methods: A total of 327 stools specimens were collected from children (n = 141) and adults (n = 186) with diarrhea attending health centers. Diarrheagenic E. coli (DEC) were identified by their virulence genes (multiplex polymerase chain reaction) and HEp-2 cell adherence patterns. Results: DEC were detected in 56 (40%) children and 74 (39%) adults
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2020-07-02T18:52:16Z
dc.date.available.fl_str_mv 2020-07-02T18:52:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv Bmc Infectious Diseases. London, v. 17, p. -, 2017.
dc.identifier.uri.fl_str_mv https://repositorio.unifesp.br/handle/11600/53989
http://dx.doi.org/10.1186/s12879-017-2872-0
dc.identifier.issn.none.fl_str_mv 1471-2334
dc.identifier.file.none.fl_str_mv WOS000418160300001.pdf
dc.identifier.doi.none.fl_str_mv 10.1186/s12879-017-2872-0
dc.identifier.wos.none.fl_str_mv WOS:000418160300001
identifier_str_mv Bmc Infectious Diseases. London, v. 17, p. -, 2017.
1471-2334
WOS000418160300001.pdf
10.1186/s12879-017-2872-0
WOS:000418160300001
url https://repositorio.unifesp.br/handle/11600/53989
http://dx.doi.org/10.1186/s12879-017-2872-0
dc.language.iso.fl_str_mv eng
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dc.relation.ispartof.none.fl_str_mv Bmc Infectious Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.coverage.none.fl_str_mv London
dc.publisher.none.fl_str_mv Biomed Central Ltd
publisher.none.fl_str_mv Biomed Central Ltd
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