Study of the density of ganglion cells in the terminal bowel of rats with anorectal malformations

Detalhes bibliográficos
Autor(a) principal: Macedo, Mauricio [UNIFESP]
Data de Publicação: 2007
Outros Autores: Martins, José Luiz [UNIFESP], Meyer, Karine Furtado [UNIFESP], Soares, Iberê Cauduro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0102-86502007000600005
http://repositorio.unifesp.br/handle/11600/4041
Resumo: PURPOSE: To study the ganglion cells (GC) in the terminal bowel of rats with ethylenethiourea (ETU) induced anorectal malformations (ARM). METHODS: The animals were divided into three groups: Group A - normal fetuses from pregnant rats that were not administered ETU; Group B - fetuses without ARM born from pregnant rats that were administered ETU and Group C - fetuses with ARM born from pregnant rats that received ETU. ETU was administered on the 11th day of pregnancy at the dose of 125 mg/kg body weight by gastric gavage. The rats had cesarean section on the 21st day of gestation. The fetuses terminal bowel tissue was analyzed by immunohistochemistry to demonstrate ganglion cells. RESULTS: Statistically significant differences were found between groups A, B and C regarding ganglion cell densities. Group A had the highest cell density, followed by Group B and the lowest density was found in Group C. CONCLUSION: Ganglion cell densities are decreased in the terminal bowel of rats with ARM.
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spelling Study of the density of ganglion cells in the terminal bowel of rats with anorectal malformationsEstudo da densidade das células ganglionares no intestino terminal de ratos portadores de anomalia anorretalAnus, ImperforateConstipationEthylenethioureaS100 proteinEnteric Nervous SystemRatsConstipação IntestinalEtilenotiouréiaProteínas S100Sistema nervoso entéricoRatosPURPOSE: To study the ganglion cells (GC) in the terminal bowel of rats with ethylenethiourea (ETU) induced anorectal malformations (ARM). METHODS: The animals were divided into three groups: Group A - normal fetuses from pregnant rats that were not administered ETU; Group B - fetuses without ARM born from pregnant rats that were administered ETU and Group C - fetuses with ARM born from pregnant rats that received ETU. ETU was administered on the 11th day of pregnancy at the dose of 125 mg/kg body weight by gastric gavage. The rats had cesarean section on the 21st day of gestation. The fetuses terminal bowel tissue was analyzed by immunohistochemistry to demonstrate ganglion cells. RESULTS: Statistically significant differences were found between groups A, B and C regarding ganglion cell densities. Group A had the highest cell density, followed by Group B and the lowest density was found in Group C. CONCLUSION: Ganglion cell densities are decreased in the terminal bowel of rats with ARM.OBJETIVO: Estudar as células ganglionares (CG) no intestino terminal de ratos portadores de anomalia anorretal (AAR) induzida pela etilenotiouréia (ETU). MÉTODOS: Os animais foram distribuídos em três grupos: Grupo A - fetos normais, obtidos de ratas grávidas às quais não foi administrada ETU; Grupo B - fetos não portadores de AAR obtidos de ratas grávidas às quais foi administrada ETU e Grupo C - fetos portadores de AAR obtidos de ratas grávidas às quais foi administrada ETU. A ETU foi administrada no décimo primeiro dia de gestação na dose de 125 mg/Kg, por gavagem. As ratas foram submetidas à laparotomia e histerotomia para retirada dos fetos no vigésimo primeiro dia de gestação. O intestino terminal dos fetos foi retirado e analisado por imunohistoquímica para pesquisa de CG. RESULTADOS: Foram encontradas diferenças estatisticamente significantes entre os grupos A, B e C quanto à densidade de CG. O grupo A apresentou a maior densidade, seguida pelo grupo B, e a menor densidade foi encontrada no Grupo C. CONCLUSÃO: Existe uma menor densidade de CG no intestino terminal de ratos portadores de AAR.UNIFESP Post-Graduate Program in Experimental SurgeryUniversidade Federal de São Paulo (UNIFESP) Department of Surgery Division of Pediatric SurgeryHospital of São Paulo (HSPE) Public ServantsUNIFESP, Post-Graduate Program in Experimental SurgeryUNIFESP, Department of Surgery Division of Pediatric SurgerySciELOSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaUniversidade Federal de São Paulo (UNIFESP)Hospital of São Paulo (HSPE) Public ServantsMacedo, Mauricio [UNIFESP]Martins, José Luiz [UNIFESP]Meyer, Karine Furtado [UNIFESP]Soares, Iberê Cauduro2015-06-14T13:37:14Z2015-06-14T13:37:14Z2007-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion441-445application/pdfhttp://dx.doi.org/10.1590/S0102-86502007000600005Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 22, n. 6, p. 441-445, 2007.10.1590/S0102-86502007000600005S0102-86502007000600005.pdf0102-8650S0102-86502007000600005http://repositorio.unifesp.br/handle/11600/4041WOS:000258573500005engActa Cirurgica Brasileirainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-06T01:39:25Zoai:repositorio.unifesp.br/:11600/4041Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-06T01:39:25Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Study of the density of ganglion cells in the terminal bowel of rats with anorectal malformations
Estudo da densidade das células ganglionares no intestino terminal de ratos portadores de anomalia anorretal
title Study of the density of ganglion cells in the terminal bowel of rats with anorectal malformations
spellingShingle Study of the density of ganglion cells in the terminal bowel of rats with anorectal malformations
Macedo, Mauricio [UNIFESP]
Anus, Imperforate
Constipation
Ethylenethiourea
S100 protein
Enteric Nervous System
Rats
Constipação Intestinal
Etilenotiouréia
Proteínas S100
Sistema nervoso entérico
Ratos
title_short Study of the density of ganglion cells in the terminal bowel of rats with anorectal malformations
title_full Study of the density of ganglion cells in the terminal bowel of rats with anorectal malformations
title_fullStr Study of the density of ganglion cells in the terminal bowel of rats with anorectal malformations
title_full_unstemmed Study of the density of ganglion cells in the terminal bowel of rats with anorectal malformations
title_sort Study of the density of ganglion cells in the terminal bowel of rats with anorectal malformations
author Macedo, Mauricio [UNIFESP]
author_facet Macedo, Mauricio [UNIFESP]
Martins, José Luiz [UNIFESP]
Meyer, Karine Furtado [UNIFESP]
Soares, Iberê Cauduro
author_role author
author2 Martins, José Luiz [UNIFESP]
Meyer, Karine Furtado [UNIFESP]
Soares, Iberê Cauduro
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Hospital of São Paulo (HSPE) Public Servants
dc.contributor.author.fl_str_mv Macedo, Mauricio [UNIFESP]
Martins, José Luiz [UNIFESP]
Meyer, Karine Furtado [UNIFESP]
Soares, Iberê Cauduro
dc.subject.por.fl_str_mv Anus, Imperforate
Constipation
Ethylenethiourea
S100 protein
Enteric Nervous System
Rats
Constipação Intestinal
Etilenotiouréia
Proteínas S100
Sistema nervoso entérico
Ratos
topic Anus, Imperforate
Constipation
Ethylenethiourea
S100 protein
Enteric Nervous System
Rats
Constipação Intestinal
Etilenotiouréia
Proteínas S100
Sistema nervoso entérico
Ratos
description PURPOSE: To study the ganglion cells (GC) in the terminal bowel of rats with ethylenethiourea (ETU) induced anorectal malformations (ARM). METHODS: The animals were divided into three groups: Group A - normal fetuses from pregnant rats that were not administered ETU; Group B - fetuses without ARM born from pregnant rats that were administered ETU and Group C - fetuses with ARM born from pregnant rats that received ETU. ETU was administered on the 11th day of pregnancy at the dose of 125 mg/kg body weight by gastric gavage. The rats had cesarean section on the 21st day of gestation. The fetuses terminal bowel tissue was analyzed by immunohistochemistry to demonstrate ganglion cells. RESULTS: Statistically significant differences were found between groups A, B and C regarding ganglion cell densities. Group A had the highest cell density, followed by Group B and the lowest density was found in Group C. CONCLUSION: Ganglion cell densities are decreased in the terminal bowel of rats with ARM.
publishDate 2007
dc.date.none.fl_str_mv 2007-12-01
2015-06-14T13:37:14Z
2015-06-14T13:37:14Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0102-86502007000600005
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 22, n. 6, p. 441-445, 2007.
10.1590/S0102-86502007000600005
S0102-86502007000600005.pdf
0102-8650
S0102-86502007000600005
http://repositorio.unifesp.br/handle/11600/4041
WOS:000258573500005
url http://dx.doi.org/10.1590/S0102-86502007000600005
http://repositorio.unifesp.br/handle/11600/4041
identifier_str_mv Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 22, n. 6, p. 441-445, 2007.
10.1590/S0102-86502007000600005
S0102-86502007000600005.pdf
0102-8650
S0102-86502007000600005
WOS:000258573500005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Cirurgica Brasileira
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 441-445
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268366514290688

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