Structure of the N-linked oligosaccharide of the main diagnostic antigen of the pathogenic fungus Paracoccidioides brasiliensis
Autor(a) principal: | |
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Data de Publicação: | 1996 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1093/glycob/6.5.507 http://repositorio.unifesp.br/handle/11600/25608 |
Resumo: | The major diagnostic antigen of Paracoccinioides brasiliensis is the exocellularly secreted 43,000 Da glycoprotein (gp43) which contains a single N-linked oligosaccharide chain, This oligosaccharide, although poorly immunogenic in man, is responsible for the cross-reactivity of the gp43 with sera from patients with histoplasmosis, and may have a role in fungal virulence, It contains a neutral high-mannose core (Man(7)GlcNAc(2)) to which a (1-->6)-linked alpha-D-Manp chain of variable length, substituted at the 2-O positions by single alpha-D-Manp residues, is attached, A terminal unit of beta-D-galactofuranose is (1-->6)-linked to one of the (1-->2)-linked mannosyl residues, either in the C or in the A arm of the oligosaccharide, the heterogeneity of the oligosaccharide is determined by the different sizes of the A arm and the sites of insertion of the beta-galactofuranosyl unit. the complete structure was determined hy methylation analysis, H-1-NMR, mass spectrometry, acetolysis and mannosidase degradation, Electrospray mass spectrometry showed that the oligosaccharide comprises several subtypes ranging from Hex(18)GlcNAc(2) to Hex(10)GlcNAc(2) which accounts for the diffuse migration of the gp43 in polyacrylamide gels, the average size of the most frequent subtype is Hex(13.6)GlcNAc(2). Dilute acid treatment to remove beta-D-Galf reduced the molecular masses of the majority of the subtypes by a single sugar unit. |
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Structure of the N-linked oligosaccharide of the main diagnostic antigen of the pathogenic fungus Paracoccidioides brasiliensisParacoccidioides brasiliensisgp43diagnostic antigenN-linked oligosaccharideThe major diagnostic antigen of Paracoccinioides brasiliensis is the exocellularly secreted 43,000 Da glycoprotein (gp43) which contains a single N-linked oligosaccharide chain, This oligosaccharide, although poorly immunogenic in man, is responsible for the cross-reactivity of the gp43 with sera from patients with histoplasmosis, and may have a role in fungal virulence, It contains a neutral high-mannose core (Man(7)GlcNAc(2)) to which a (1-->6)-linked alpha-D-Manp chain of variable length, substituted at the 2-O positions by single alpha-D-Manp residues, is attached, A terminal unit of beta-D-galactofuranose is (1-->6)-linked to one of the (1-->2)-linked mannosyl residues, either in the C or in the A arm of the oligosaccharide, the heterogeneity of the oligosaccharide is determined by the different sizes of the A arm and the sites of insertion of the beta-galactofuranosyl unit. the complete structure was determined hy methylation analysis, H-1-NMR, mass spectrometry, acetolysis and mannosidase degradation, Electrospray mass spectrometry showed that the oligosaccharide comprises several subtypes ranging from Hex(18)GlcNAc(2) to Hex(10)GlcNAc(2) which accounts for the diffuse migration of the gp43 in polyacrylamide gels, the average size of the most frequent subtype is Hex(13.6)GlcNAc(2). Dilute acid treatment to remove beta-D-Galf reduced the molecular masses of the majority of the subtypes by a single sugar unit.Universidade Federal de São Paulo,DISCIPLINA BIOL CELULAR,ESCOLA PAULISTA MED,BR-04023 São Paulo,BRAZILUNIV DUNDEE,DEPT BIOCHEM,DUNDEE DD1 4HN,SCOTLANDFED UNIV RIO de JANEIRO,INST MICROBIOL,RIO JANEIRO,BRAZILUniversidade Federal de São Paulo,DISCIPLINA BIOL CELULAR,ESCOLA PAULISTA MED,BR-04023 São Paulo,BRAZILWeb of ScienceOxford Univ Press United KingdomUniversidade Federal de São Paulo (UNIFESP)UNIV DUNDEEUniversidade Federal do Rio de Janeiro (UFRJ)Almeida, Igor C [UNIFESP]Neville, DCAMehlert, A.Treumann, A.Ferguson, MAJPreviato, J. O.Travassos, L. R.2016-01-24T11:40:33Z2016-01-24T11:40:33Z1996-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion507-515http://dx.doi.org/10.1093/glycob/6.5.507Glycobiology. Oxford: Oxford Univ Press United Kingdom, v. 6, n. 5, p. 507-515, 1996.10.1093/glycob/6.5.5070959-6658http://repositorio.unifesp.br/handle/11600/25608WOS:A1996VF56600008engGlycobiologyinfo:eu-repo/semantics/openAccesshttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.htmlreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2023-05-18T13:50:59Zoai:repositorio.unifesp.br/:11600/25608Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652023-05-18T13:50:59Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Structure of the N-linked oligosaccharide of the main diagnostic antigen of the pathogenic fungus Paracoccidioides brasiliensis |
title |
Structure of the N-linked oligosaccharide of the main diagnostic antigen of the pathogenic fungus Paracoccidioides brasiliensis |
spellingShingle |
Structure of the N-linked oligosaccharide of the main diagnostic antigen of the pathogenic fungus Paracoccidioides brasiliensis Almeida, Igor C [UNIFESP] Paracoccidioides brasiliensis gp43 diagnostic antigen N-linked oligosaccharide |
title_short |
Structure of the N-linked oligosaccharide of the main diagnostic antigen of the pathogenic fungus Paracoccidioides brasiliensis |
title_full |
Structure of the N-linked oligosaccharide of the main diagnostic antigen of the pathogenic fungus Paracoccidioides brasiliensis |
title_fullStr |
Structure of the N-linked oligosaccharide of the main diagnostic antigen of the pathogenic fungus Paracoccidioides brasiliensis |
title_full_unstemmed |
Structure of the N-linked oligosaccharide of the main diagnostic antigen of the pathogenic fungus Paracoccidioides brasiliensis |
title_sort |
Structure of the N-linked oligosaccharide of the main diagnostic antigen of the pathogenic fungus Paracoccidioides brasiliensis |
author |
Almeida, Igor C [UNIFESP] |
author_facet |
Almeida, Igor C [UNIFESP] Neville, DCA Mehlert, A. Treumann, A. Ferguson, MAJ Previato, J. O. Travassos, L. R. |
author_role |
author |
author2 |
Neville, DCA Mehlert, A. Treumann, A. Ferguson, MAJ Previato, J. O. Travassos, L. R. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) UNIV DUNDEE Universidade Federal do Rio de Janeiro (UFRJ) |
dc.contributor.author.fl_str_mv |
Almeida, Igor C [UNIFESP] Neville, DCA Mehlert, A. Treumann, A. Ferguson, MAJ Previato, J. O. Travassos, L. R. |
dc.subject.por.fl_str_mv |
Paracoccidioides brasiliensis gp43 diagnostic antigen N-linked oligosaccharide |
topic |
Paracoccidioides brasiliensis gp43 diagnostic antigen N-linked oligosaccharide |
description |
The major diagnostic antigen of Paracoccinioides brasiliensis is the exocellularly secreted 43,000 Da glycoprotein (gp43) which contains a single N-linked oligosaccharide chain, This oligosaccharide, although poorly immunogenic in man, is responsible for the cross-reactivity of the gp43 with sera from patients with histoplasmosis, and may have a role in fungal virulence, It contains a neutral high-mannose core (Man(7)GlcNAc(2)) to which a (1-->6)-linked alpha-D-Manp chain of variable length, substituted at the 2-O positions by single alpha-D-Manp residues, is attached, A terminal unit of beta-D-galactofuranose is (1-->6)-linked to one of the (1-->2)-linked mannosyl residues, either in the C or in the A arm of the oligosaccharide, the heterogeneity of the oligosaccharide is determined by the different sizes of the A arm and the sites of insertion of the beta-galactofuranosyl unit. the complete structure was determined hy methylation analysis, H-1-NMR, mass spectrometry, acetolysis and mannosidase degradation, Electrospray mass spectrometry showed that the oligosaccharide comprises several subtypes ranging from Hex(18)GlcNAc(2) to Hex(10)GlcNAc(2) which accounts for the diffuse migration of the gp43 in polyacrylamide gels, the average size of the most frequent subtype is Hex(13.6)GlcNAc(2). Dilute acid treatment to remove beta-D-Galf reduced the molecular masses of the majority of the subtypes by a single sugar unit. |
publishDate |
1996 |
dc.date.none.fl_str_mv |
1996-07-01 2016-01-24T11:40:33Z 2016-01-24T11:40:33Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/glycob/6.5.507 Glycobiology. Oxford: Oxford Univ Press United Kingdom, v. 6, n. 5, p. 507-515, 1996. 10.1093/glycob/6.5.507 0959-6658 http://repositorio.unifesp.br/handle/11600/25608 WOS:A1996VF56600008 |
url |
http://dx.doi.org/10.1093/glycob/6.5.507 http://repositorio.unifesp.br/handle/11600/25608 |
identifier_str_mv |
Glycobiology. Oxford: Oxford Univ Press United Kingdom, v. 6, n. 5, p. 507-515, 1996. 10.1093/glycob/6.5.507 0959-6658 WOS:A1996VF56600008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Glycobiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html |
dc.format.none.fl_str_mv |
507-515 |
dc.publisher.none.fl_str_mv |
Oxford Univ Press United Kingdom |
publisher.none.fl_str_mv |
Oxford Univ Press United Kingdom |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268343052402688 |