Structure of the N-linked oligosaccharide of the main diagnostic antigen of the pathogenic fungus Paracoccidioides brasiliensis

Detalhes bibliográficos
Autor(a) principal: Almeida, Igor C [UNIFESP]
Data de Publicação: 1996
Outros Autores: Neville, DCA, Mehlert, A., Treumann, A., Ferguson, MAJ, Previato, J. O., Travassos, L. R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1093/glycob/6.5.507
http://repositorio.unifesp.br/handle/11600/25608
Resumo: The major diagnostic antigen of Paracoccinioides brasiliensis is the exocellularly secreted 43,000 Da glycoprotein (gp43) which contains a single N-linked oligosaccharide chain, This oligosaccharide, although poorly immunogenic in man, is responsible for the cross-reactivity of the gp43 with sera from patients with histoplasmosis, and may have a role in fungal virulence, It contains a neutral high-mannose core (Man(7)GlcNAc(2)) to which a (1-->6)-linked alpha-D-Manp chain of variable length, substituted at the 2-O positions by single alpha-D-Manp residues, is attached, A terminal unit of beta-D-galactofuranose is (1-->6)-linked to one of the (1-->2)-linked mannosyl residues, either in the C or in the A arm of the oligosaccharide, the heterogeneity of the oligosaccharide is determined by the different sizes of the A arm and the sites of insertion of the beta-galactofuranosyl unit. the complete structure was determined hy methylation analysis, H-1-NMR, mass spectrometry, acetolysis and mannosidase degradation, Electrospray mass spectrometry showed that the oligosaccharide comprises several subtypes ranging from Hex(18)GlcNAc(2) to Hex(10)GlcNAc(2) which accounts for the diffuse migration of the gp43 in polyacrylamide gels, the average size of the most frequent subtype is Hex(13.6)GlcNAc(2). Dilute acid treatment to remove beta-D-Galf reduced the molecular masses of the majority of the subtypes by a single sugar unit.
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spelling Structure of the N-linked oligosaccharide of the main diagnostic antigen of the pathogenic fungus Paracoccidioides brasiliensisParacoccidioides brasiliensisgp43diagnostic antigenN-linked oligosaccharideThe major diagnostic antigen of Paracoccinioides brasiliensis is the exocellularly secreted 43,000 Da glycoprotein (gp43) which contains a single N-linked oligosaccharide chain, This oligosaccharide, although poorly immunogenic in man, is responsible for the cross-reactivity of the gp43 with sera from patients with histoplasmosis, and may have a role in fungal virulence, It contains a neutral high-mannose core (Man(7)GlcNAc(2)) to which a (1-->6)-linked alpha-D-Manp chain of variable length, substituted at the 2-O positions by single alpha-D-Manp residues, is attached, A terminal unit of beta-D-galactofuranose is (1-->6)-linked to one of the (1-->2)-linked mannosyl residues, either in the C or in the A arm of the oligosaccharide, the heterogeneity of the oligosaccharide is determined by the different sizes of the A arm and the sites of insertion of the beta-galactofuranosyl unit. the complete structure was determined hy methylation analysis, H-1-NMR, mass spectrometry, acetolysis and mannosidase degradation, Electrospray mass spectrometry showed that the oligosaccharide comprises several subtypes ranging from Hex(18)GlcNAc(2) to Hex(10)GlcNAc(2) which accounts for the diffuse migration of the gp43 in polyacrylamide gels, the average size of the most frequent subtype is Hex(13.6)GlcNAc(2). Dilute acid treatment to remove beta-D-Galf reduced the molecular masses of the majority of the subtypes by a single sugar unit.Universidade Federal de São Paulo,DISCIPLINA BIOL CELULAR,ESCOLA PAULISTA MED,BR-04023 São Paulo,BRAZILUNIV DUNDEE,DEPT BIOCHEM,DUNDEE DD1 4HN,SCOTLANDFED UNIV RIO de JANEIRO,INST MICROBIOL,RIO JANEIRO,BRAZILUniversidade Federal de São Paulo,DISCIPLINA BIOL CELULAR,ESCOLA PAULISTA MED,BR-04023 São Paulo,BRAZILWeb of ScienceOxford Univ Press United KingdomUniversidade Federal de São Paulo (UNIFESP)UNIV DUNDEEUniversidade Federal do Rio de Janeiro (UFRJ)Almeida, Igor C [UNIFESP]Neville, DCAMehlert, A.Treumann, A.Ferguson, MAJPreviato, J. O.Travassos, L. R.2016-01-24T11:40:33Z2016-01-24T11:40:33Z1996-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion507-515http://dx.doi.org/10.1093/glycob/6.5.507Glycobiology. Oxford: Oxford Univ Press United Kingdom, v. 6, n. 5, p. 507-515, 1996.10.1093/glycob/6.5.5070959-6658http://repositorio.unifesp.br/handle/11600/25608WOS:A1996VF56600008engGlycobiologyinfo:eu-repo/semantics/openAccesshttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.htmlreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2023-05-18T13:50:59Zoai:repositorio.unifesp.br/:11600/25608Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652023-05-18T13:50:59Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Structure of the N-linked oligosaccharide of the main diagnostic antigen of the pathogenic fungus Paracoccidioides brasiliensis
title Structure of the N-linked oligosaccharide of the main diagnostic antigen of the pathogenic fungus Paracoccidioides brasiliensis
spellingShingle Structure of the N-linked oligosaccharide of the main diagnostic antigen of the pathogenic fungus Paracoccidioides brasiliensis
Almeida, Igor C [UNIFESP]
Paracoccidioides brasiliensis
gp43
diagnostic antigen
N-linked oligosaccharide
title_short Structure of the N-linked oligosaccharide of the main diagnostic antigen of the pathogenic fungus Paracoccidioides brasiliensis
title_full Structure of the N-linked oligosaccharide of the main diagnostic antigen of the pathogenic fungus Paracoccidioides brasiliensis
title_fullStr Structure of the N-linked oligosaccharide of the main diagnostic antigen of the pathogenic fungus Paracoccidioides brasiliensis
title_full_unstemmed Structure of the N-linked oligosaccharide of the main diagnostic antigen of the pathogenic fungus Paracoccidioides brasiliensis
title_sort Structure of the N-linked oligosaccharide of the main diagnostic antigen of the pathogenic fungus Paracoccidioides brasiliensis
author Almeida, Igor C [UNIFESP]
author_facet Almeida, Igor C [UNIFESP]
Neville, DCA
Mehlert, A.
Treumann, A.
Ferguson, MAJ
Previato, J. O.
Travassos, L. R.
author_role author
author2 Neville, DCA
Mehlert, A.
Treumann, A.
Ferguson, MAJ
Previato, J. O.
Travassos, L. R.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
UNIV DUNDEE
Universidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.author.fl_str_mv Almeida, Igor C [UNIFESP]
Neville, DCA
Mehlert, A.
Treumann, A.
Ferguson, MAJ
Previato, J. O.
Travassos, L. R.
dc.subject.por.fl_str_mv Paracoccidioides brasiliensis
gp43
diagnostic antigen
N-linked oligosaccharide
topic Paracoccidioides brasiliensis
gp43
diagnostic antigen
N-linked oligosaccharide
description The major diagnostic antigen of Paracoccinioides brasiliensis is the exocellularly secreted 43,000 Da glycoprotein (gp43) which contains a single N-linked oligosaccharide chain, This oligosaccharide, although poorly immunogenic in man, is responsible for the cross-reactivity of the gp43 with sera from patients with histoplasmosis, and may have a role in fungal virulence, It contains a neutral high-mannose core (Man(7)GlcNAc(2)) to which a (1-->6)-linked alpha-D-Manp chain of variable length, substituted at the 2-O positions by single alpha-D-Manp residues, is attached, A terminal unit of beta-D-galactofuranose is (1-->6)-linked to one of the (1-->2)-linked mannosyl residues, either in the C or in the A arm of the oligosaccharide, the heterogeneity of the oligosaccharide is determined by the different sizes of the A arm and the sites of insertion of the beta-galactofuranosyl unit. the complete structure was determined hy methylation analysis, H-1-NMR, mass spectrometry, acetolysis and mannosidase degradation, Electrospray mass spectrometry showed that the oligosaccharide comprises several subtypes ranging from Hex(18)GlcNAc(2) to Hex(10)GlcNAc(2) which accounts for the diffuse migration of the gp43 in polyacrylamide gels, the average size of the most frequent subtype is Hex(13.6)GlcNAc(2). Dilute acid treatment to remove beta-D-Galf reduced the molecular masses of the majority of the subtypes by a single sugar unit.
publishDate 1996
dc.date.none.fl_str_mv 1996-07-01
2016-01-24T11:40:33Z
2016-01-24T11:40:33Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1093/glycob/6.5.507
Glycobiology. Oxford: Oxford Univ Press United Kingdom, v. 6, n. 5, p. 507-515, 1996.
10.1093/glycob/6.5.507
0959-6658
http://repositorio.unifesp.br/handle/11600/25608
WOS:A1996VF56600008
url http://dx.doi.org/10.1093/glycob/6.5.507
http://repositorio.unifesp.br/handle/11600/25608
identifier_str_mv Glycobiology. Oxford: Oxford Univ Press United Kingdom, v. 6, n. 5, p. 507-515, 1996.
10.1093/glycob/6.5.507
0959-6658
WOS:A1996VF56600008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Glycobiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dc.format.none.fl_str_mv 507-515
dc.publisher.none.fl_str_mv Oxford Univ Press United Kingdom
publisher.none.fl_str_mv Oxford Univ Press United Kingdom
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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