Changes in the seminal plasma proteome of adolescents before and after varicocelectomy

Detalhes bibliográficos
Autor(a) principal: Del Giudice, Paula Toni [UNIFESP]
Data de Publicação: 2013
Outros Autores: Silva, Barbara Ferreira da [UNIFESP], Lo Turco, Edson Guimaraes [UNIFESP], Fraietta, Renato [UNIFESP], Spaine, Deborah Montagnini [UNIFESP], Santos, Luiz Fernando Arruda, Pilau, Eduardo Jorge, Gozzo, Fabio Cesar, Cedenho, Agnaldo Pereira [UNIFESP], Bertolla, Ricardo Pimenta [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1016/j.fertnstert.2013.04.036
http://repositorio.unifesp.br/handle/11600/36661
Resumo: Objective: To compare seminal plasma protein profiles before and after varicocele correction to assess if surgical intervention alters the protein profile.Design: Prospective study.Setting: Academic research environment.Patient(s): Nineteen adolescent boys with varicocele grades II or III.Intervention(s): Two semen samples were collected before bilateral subinguinal microsurgical varicocelectomy, and two semen samples were collected 3 months after surgery. Seminal plasma protein profiles were determined with the use of two-dimensional gel electrophoresis. Proteins were separated in 18-cm 3-10 pH strips and 10%-17.5% gradient gels. Gels were stained, scanned, and compared with the use of Imagemaster 2D platinum 7.0. Spots of interest were removed from gels, and protein digestion was performed with the use of trypsin. Digests were identified with the use of electrospray ionization-quadrupole/time-of-flight tandem mass spectrometry (ESI-QTOF MS/MS), and spectra were analyzed with the use of the Mascot software.Main Outcome Measure(s): Proteins uniquely or overexpressed in each period (before or after varicocelectomy).Result(s): Nineteen spots were differentially expressed between pre- and postsurgery samples. Identified proteins were albumin, proteasome subunit alpha type 6, alpha-1-antitrypsin, fibronectin, CD177, prostatic acid phosphatase, specific prostatic antigen, alpha-2antiplasmin, vitamin D-binding protein, gastricsin, clusterin, semenogelin-1, semenogelin-2, superoxide dismutase, protein-glutamine gamma glutamyltransferase-4, and prolactin-inducing protein.Conclusion(s): Varicocelectomy is associated with changes in the seminal plasma protein profile. Understanding specific pathways leading to male infertility may further assist physicians in demonstrating deviation from homeostasis in male infertility. in addition, it may be possible to observe if surgical intervention does indeed revert altered pathways toward a homeostatic state. ((C) 2013 by American Society for Reproductive Medicine.)
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spelling Changes in the seminal plasma proteome of adolescents before and after varicocelectomyAdolescentsproteomeseminal plasmavaricocelevaricocelectomyObjective: To compare seminal plasma protein profiles before and after varicocele correction to assess if surgical intervention alters the protein profile.Design: Prospective study.Setting: Academic research environment.Patient(s): Nineteen adolescent boys with varicocele grades II or III.Intervention(s): Two semen samples were collected before bilateral subinguinal microsurgical varicocelectomy, and two semen samples were collected 3 months after surgery. Seminal plasma protein profiles were determined with the use of two-dimensional gel electrophoresis. Proteins were separated in 18-cm 3-10 pH strips and 10%-17.5% gradient gels. Gels were stained, scanned, and compared with the use of Imagemaster 2D platinum 7.0. Spots of interest were removed from gels, and protein digestion was performed with the use of trypsin. Digests were identified with the use of electrospray ionization-quadrupole/time-of-flight tandem mass spectrometry (ESI-QTOF MS/MS), and spectra were analyzed with the use of the Mascot software.Main Outcome Measure(s): Proteins uniquely or overexpressed in each period (before or after varicocelectomy).Result(s): Nineteen spots were differentially expressed between pre- and postsurgery samples. Identified proteins were albumin, proteasome subunit alpha type 6, alpha-1-antitrypsin, fibronectin, CD177, prostatic acid phosphatase, specific prostatic antigen, alpha-2antiplasmin, vitamin D-binding protein, gastricsin, clusterin, semenogelin-1, semenogelin-2, superoxide dismutase, protein-glutamine gamma glutamyltransferase-4, and prolactin-inducing protein.Conclusion(s): Varicocelectomy is associated with changes in the seminal plasma protein profile. Understanding specific pathways leading to male infertility may further assist physicians in demonstrating deviation from homeostasis in male infertility. in addition, it may be possible to observe if surgical intervention does indeed revert altered pathways toward a homeostatic state. ((C) 2013 by American Society for Reproductive Medicine.)Universidade Federal de São Paulo, Dept Surg, Div Urol, Human Reprod Sect, BR-04039060 São Paulo, BrazilUniv Estadual Campinas, Inst Chem, São Paulo, BrazilInst Nacl Ciencia & Tecnol Bioanalit, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Surg, Div Urol, Human Reprod Sect, BR-04039060 São Paulo, BrazilWeb of ScienceElsevier B.V.Universidade Federal de São Paulo (UNIFESP)Universidade Estadual de Campinas (UNICAMP)Inst Nacl Ciencia & Tecnol BioanalitDel Giudice, Paula Toni [UNIFESP]Silva, Barbara Ferreira da [UNIFESP]Lo Turco, Edson Guimaraes [UNIFESP]Fraietta, Renato [UNIFESP]Spaine, Deborah Montagnini [UNIFESP]Santos, Luiz Fernando ArrudaPilau, Eduardo JorgeGozzo, Fabio CesarCedenho, Agnaldo Pereira [UNIFESP]Bertolla, Ricardo Pimenta [UNIFESP]2016-01-24T14:34:18Z2016-01-24T14:34:18Z2013-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion667-672http://dx.doi.org/10.1016/j.fertnstert.2013.04.036Fertility and Sterility. New York: Elsevier B.V., v. 100, n. 3, p. 667-672, 2013.10.1016/j.fertnstert.2013.04.0360015-0282http://repositorio.unifesp.br/handle/11600/36661WOS:000324234900024engFertility and Sterilityinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2016-01-24T12:34:18Zoai:repositorio.unifesp.br/:11600/36661Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652016-01-24T12:34:18Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Changes in the seminal plasma proteome of adolescents before and after varicocelectomy
title Changes in the seminal plasma proteome of adolescents before and after varicocelectomy
spellingShingle Changes in the seminal plasma proteome of adolescents before and after varicocelectomy
Del Giudice, Paula Toni [UNIFESP]
Adolescents
proteome
seminal plasma
varicocele
varicocelectomy
title_short Changes in the seminal plasma proteome of adolescents before and after varicocelectomy
title_full Changes in the seminal plasma proteome of adolescents before and after varicocelectomy
title_fullStr Changes in the seminal plasma proteome of adolescents before and after varicocelectomy
title_full_unstemmed Changes in the seminal plasma proteome of adolescents before and after varicocelectomy
title_sort Changes in the seminal plasma proteome of adolescents before and after varicocelectomy
author Del Giudice, Paula Toni [UNIFESP]
author_facet Del Giudice, Paula Toni [UNIFESP]
Silva, Barbara Ferreira da [UNIFESP]
Lo Turco, Edson Guimaraes [UNIFESP]
Fraietta, Renato [UNIFESP]
Spaine, Deborah Montagnini [UNIFESP]
Santos, Luiz Fernando Arruda
Pilau, Eduardo Jorge
Gozzo, Fabio Cesar
Cedenho, Agnaldo Pereira [UNIFESP]
Bertolla, Ricardo Pimenta [UNIFESP]
author_role author
author2 Silva, Barbara Ferreira da [UNIFESP]
Lo Turco, Edson Guimaraes [UNIFESP]
Fraietta, Renato [UNIFESP]
Spaine, Deborah Montagnini [UNIFESP]
Santos, Luiz Fernando Arruda
Pilau, Eduardo Jorge
Gozzo, Fabio Cesar
Cedenho, Agnaldo Pereira [UNIFESP]
Bertolla, Ricardo Pimenta [UNIFESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
Inst Nacl Ciencia & Tecnol Bioanalit
dc.contributor.author.fl_str_mv Del Giudice, Paula Toni [UNIFESP]
Silva, Barbara Ferreira da [UNIFESP]
Lo Turco, Edson Guimaraes [UNIFESP]
Fraietta, Renato [UNIFESP]
Spaine, Deborah Montagnini [UNIFESP]
Santos, Luiz Fernando Arruda
Pilau, Eduardo Jorge
Gozzo, Fabio Cesar
Cedenho, Agnaldo Pereira [UNIFESP]
Bertolla, Ricardo Pimenta [UNIFESP]
dc.subject.por.fl_str_mv Adolescents
proteome
seminal plasma
varicocele
varicocelectomy
topic Adolescents
proteome
seminal plasma
varicocele
varicocelectomy
description Objective: To compare seminal plasma protein profiles before and after varicocele correction to assess if surgical intervention alters the protein profile.Design: Prospective study.Setting: Academic research environment.Patient(s): Nineteen adolescent boys with varicocele grades II or III.Intervention(s): Two semen samples were collected before bilateral subinguinal microsurgical varicocelectomy, and two semen samples were collected 3 months after surgery. Seminal plasma protein profiles were determined with the use of two-dimensional gel electrophoresis. Proteins were separated in 18-cm 3-10 pH strips and 10%-17.5% gradient gels. Gels were stained, scanned, and compared with the use of Imagemaster 2D platinum 7.0. Spots of interest were removed from gels, and protein digestion was performed with the use of trypsin. Digests were identified with the use of electrospray ionization-quadrupole/time-of-flight tandem mass spectrometry (ESI-QTOF MS/MS), and spectra were analyzed with the use of the Mascot software.Main Outcome Measure(s): Proteins uniquely or overexpressed in each period (before or after varicocelectomy).Result(s): Nineteen spots were differentially expressed between pre- and postsurgery samples. Identified proteins were albumin, proteasome subunit alpha type 6, alpha-1-antitrypsin, fibronectin, CD177, prostatic acid phosphatase, specific prostatic antigen, alpha-2antiplasmin, vitamin D-binding protein, gastricsin, clusterin, semenogelin-1, semenogelin-2, superoxide dismutase, protein-glutamine gamma glutamyltransferase-4, and prolactin-inducing protein.Conclusion(s): Varicocelectomy is associated with changes in the seminal plasma protein profile. Understanding specific pathways leading to male infertility may further assist physicians in demonstrating deviation from homeostasis in male infertility. in addition, it may be possible to observe if surgical intervention does indeed revert altered pathways toward a homeostatic state. ((C) 2013 by American Society for Reproductive Medicine.)
publishDate 2013
dc.date.none.fl_str_mv 2013-09-01
2016-01-24T14:34:18Z
2016-01-24T14:34:18Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.fertnstert.2013.04.036
Fertility and Sterility. New York: Elsevier B.V., v. 100, n. 3, p. 667-672, 2013.
10.1016/j.fertnstert.2013.04.036
0015-0282
http://repositorio.unifesp.br/handle/11600/36661
WOS:000324234900024
url http://dx.doi.org/10.1016/j.fertnstert.2013.04.036
http://repositorio.unifesp.br/handle/11600/36661
identifier_str_mv Fertility and Sterility. New York: Elsevier B.V., v. 100, n. 3, p. 667-672, 2013.
10.1016/j.fertnstert.2013.04.036
0015-0282
WOS:000324234900024
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Fertility and Sterility
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.format.none.fl_str_mv 667-672
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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