Changes in the seminal plasma proteome of adolescents before and after varicocelectomy
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1016/j.fertnstert.2013.04.036 http://repositorio.unifesp.br/handle/11600/36661 |
Resumo: | Objective: To compare seminal plasma protein profiles before and after varicocele correction to assess if surgical intervention alters the protein profile.Design: Prospective study.Setting: Academic research environment.Patient(s): Nineteen adolescent boys with varicocele grades II or III.Intervention(s): Two semen samples were collected before bilateral subinguinal microsurgical varicocelectomy, and two semen samples were collected 3 months after surgery. Seminal plasma protein profiles were determined with the use of two-dimensional gel electrophoresis. Proteins were separated in 18-cm 3-10 pH strips and 10%-17.5% gradient gels. Gels were stained, scanned, and compared with the use of Imagemaster 2D platinum 7.0. Spots of interest were removed from gels, and protein digestion was performed with the use of trypsin. Digests were identified with the use of electrospray ionization-quadrupole/time-of-flight tandem mass spectrometry (ESI-QTOF MS/MS), and spectra were analyzed with the use of the Mascot software.Main Outcome Measure(s): Proteins uniquely or overexpressed in each period (before or after varicocelectomy).Result(s): Nineteen spots were differentially expressed between pre- and postsurgery samples. Identified proteins were albumin, proteasome subunit alpha type 6, alpha-1-antitrypsin, fibronectin, CD177, prostatic acid phosphatase, specific prostatic antigen, alpha-2antiplasmin, vitamin D-binding protein, gastricsin, clusterin, semenogelin-1, semenogelin-2, superoxide dismutase, protein-glutamine gamma glutamyltransferase-4, and prolactin-inducing protein.Conclusion(s): Varicocelectomy is associated with changes in the seminal plasma protein profile. Understanding specific pathways leading to male infertility may further assist physicians in demonstrating deviation from homeostasis in male infertility. in addition, it may be possible to observe if surgical intervention does indeed revert altered pathways toward a homeostatic state. ((C) 2013 by American Society for Reproductive Medicine.) |
id |
UFSP_6207deffe0f0232eabf79da6284da907 |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br/:11600/36661 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Changes in the seminal plasma proteome of adolescents before and after varicocelectomyAdolescentsproteomeseminal plasmavaricocelevaricocelectomyObjective: To compare seminal plasma protein profiles before and after varicocele correction to assess if surgical intervention alters the protein profile.Design: Prospective study.Setting: Academic research environment.Patient(s): Nineteen adolescent boys with varicocele grades II or III.Intervention(s): Two semen samples were collected before bilateral subinguinal microsurgical varicocelectomy, and two semen samples were collected 3 months after surgery. Seminal plasma protein profiles were determined with the use of two-dimensional gel electrophoresis. Proteins were separated in 18-cm 3-10 pH strips and 10%-17.5% gradient gels. Gels were stained, scanned, and compared with the use of Imagemaster 2D platinum 7.0. Spots of interest were removed from gels, and protein digestion was performed with the use of trypsin. Digests were identified with the use of electrospray ionization-quadrupole/time-of-flight tandem mass spectrometry (ESI-QTOF MS/MS), and spectra were analyzed with the use of the Mascot software.Main Outcome Measure(s): Proteins uniquely or overexpressed in each period (before or after varicocelectomy).Result(s): Nineteen spots were differentially expressed between pre- and postsurgery samples. Identified proteins were albumin, proteasome subunit alpha type 6, alpha-1-antitrypsin, fibronectin, CD177, prostatic acid phosphatase, specific prostatic antigen, alpha-2antiplasmin, vitamin D-binding protein, gastricsin, clusterin, semenogelin-1, semenogelin-2, superoxide dismutase, protein-glutamine gamma glutamyltransferase-4, and prolactin-inducing protein.Conclusion(s): Varicocelectomy is associated with changes in the seminal plasma protein profile. Understanding specific pathways leading to male infertility may further assist physicians in demonstrating deviation from homeostasis in male infertility. in addition, it may be possible to observe if surgical intervention does indeed revert altered pathways toward a homeostatic state. ((C) 2013 by American Society for Reproductive Medicine.)Universidade Federal de São Paulo, Dept Surg, Div Urol, Human Reprod Sect, BR-04039060 São Paulo, BrazilUniv Estadual Campinas, Inst Chem, São Paulo, BrazilInst Nacl Ciencia & Tecnol Bioanalit, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Surg, Div Urol, Human Reprod Sect, BR-04039060 São Paulo, BrazilWeb of ScienceElsevier B.V.Universidade Federal de São Paulo (UNIFESP)Universidade Estadual de Campinas (UNICAMP)Inst Nacl Ciencia & Tecnol BioanalitDel Giudice, Paula Toni [UNIFESP]Silva, Barbara Ferreira da [UNIFESP]Lo Turco, Edson Guimaraes [UNIFESP]Fraietta, Renato [UNIFESP]Spaine, Deborah Montagnini [UNIFESP]Santos, Luiz Fernando ArrudaPilau, Eduardo JorgeGozzo, Fabio CesarCedenho, Agnaldo Pereira [UNIFESP]Bertolla, Ricardo Pimenta [UNIFESP]2016-01-24T14:34:18Z2016-01-24T14:34:18Z2013-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion667-672http://dx.doi.org/10.1016/j.fertnstert.2013.04.036Fertility and Sterility. New York: Elsevier B.V., v. 100, n. 3, p. 667-672, 2013.10.1016/j.fertnstert.2013.04.0360015-0282http://repositorio.unifesp.br/handle/11600/36661WOS:000324234900024engFertility and Sterilityinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2016-01-24T12:34:18Zoai:repositorio.unifesp.br/:11600/36661Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652016-01-24T12:34:18Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Changes in the seminal plasma proteome of adolescents before and after varicocelectomy |
title |
Changes in the seminal plasma proteome of adolescents before and after varicocelectomy |
spellingShingle |
Changes in the seminal plasma proteome of adolescents before and after varicocelectomy Del Giudice, Paula Toni [UNIFESP] Adolescents proteome seminal plasma varicocele varicocelectomy |
title_short |
Changes in the seminal plasma proteome of adolescents before and after varicocelectomy |
title_full |
Changes in the seminal plasma proteome of adolescents before and after varicocelectomy |
title_fullStr |
Changes in the seminal plasma proteome of adolescents before and after varicocelectomy |
title_full_unstemmed |
Changes in the seminal plasma proteome of adolescents before and after varicocelectomy |
title_sort |
Changes in the seminal plasma proteome of adolescents before and after varicocelectomy |
author |
Del Giudice, Paula Toni [UNIFESP] |
author_facet |
Del Giudice, Paula Toni [UNIFESP] Silva, Barbara Ferreira da [UNIFESP] Lo Turco, Edson Guimaraes [UNIFESP] Fraietta, Renato [UNIFESP] Spaine, Deborah Montagnini [UNIFESP] Santos, Luiz Fernando Arruda Pilau, Eduardo Jorge Gozzo, Fabio Cesar Cedenho, Agnaldo Pereira [UNIFESP] Bertolla, Ricardo Pimenta [UNIFESP] |
author_role |
author |
author2 |
Silva, Barbara Ferreira da [UNIFESP] Lo Turco, Edson Guimaraes [UNIFESP] Fraietta, Renato [UNIFESP] Spaine, Deborah Montagnini [UNIFESP] Santos, Luiz Fernando Arruda Pilau, Eduardo Jorge Gozzo, Fabio Cesar Cedenho, Agnaldo Pereira [UNIFESP] Bertolla, Ricardo Pimenta [UNIFESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade Estadual de Campinas (UNICAMP) Inst Nacl Ciencia & Tecnol Bioanalit |
dc.contributor.author.fl_str_mv |
Del Giudice, Paula Toni [UNIFESP] Silva, Barbara Ferreira da [UNIFESP] Lo Turco, Edson Guimaraes [UNIFESP] Fraietta, Renato [UNIFESP] Spaine, Deborah Montagnini [UNIFESP] Santos, Luiz Fernando Arruda Pilau, Eduardo Jorge Gozzo, Fabio Cesar Cedenho, Agnaldo Pereira [UNIFESP] Bertolla, Ricardo Pimenta [UNIFESP] |
dc.subject.por.fl_str_mv |
Adolescents proteome seminal plasma varicocele varicocelectomy |
topic |
Adolescents proteome seminal plasma varicocele varicocelectomy |
description |
Objective: To compare seminal plasma protein profiles before and after varicocele correction to assess if surgical intervention alters the protein profile.Design: Prospective study.Setting: Academic research environment.Patient(s): Nineteen adolescent boys with varicocele grades II or III.Intervention(s): Two semen samples were collected before bilateral subinguinal microsurgical varicocelectomy, and two semen samples were collected 3 months after surgery. Seminal plasma protein profiles were determined with the use of two-dimensional gel electrophoresis. Proteins were separated in 18-cm 3-10 pH strips and 10%-17.5% gradient gels. Gels were stained, scanned, and compared with the use of Imagemaster 2D platinum 7.0. Spots of interest were removed from gels, and protein digestion was performed with the use of trypsin. Digests were identified with the use of electrospray ionization-quadrupole/time-of-flight tandem mass spectrometry (ESI-QTOF MS/MS), and spectra were analyzed with the use of the Mascot software.Main Outcome Measure(s): Proteins uniquely or overexpressed in each period (before or after varicocelectomy).Result(s): Nineteen spots were differentially expressed between pre- and postsurgery samples. Identified proteins were albumin, proteasome subunit alpha type 6, alpha-1-antitrypsin, fibronectin, CD177, prostatic acid phosphatase, specific prostatic antigen, alpha-2antiplasmin, vitamin D-binding protein, gastricsin, clusterin, semenogelin-1, semenogelin-2, superoxide dismutase, protein-glutamine gamma glutamyltransferase-4, and prolactin-inducing protein.Conclusion(s): Varicocelectomy is associated with changes in the seminal plasma protein profile. Understanding specific pathways leading to male infertility may further assist physicians in demonstrating deviation from homeostasis in male infertility. in addition, it may be possible to observe if surgical intervention does indeed revert altered pathways toward a homeostatic state. ((C) 2013 by American Society for Reproductive Medicine.) |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-09-01 2016-01-24T14:34:18Z 2016-01-24T14:34:18Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.fertnstert.2013.04.036 Fertility and Sterility. New York: Elsevier B.V., v. 100, n. 3, p. 667-672, 2013. 10.1016/j.fertnstert.2013.04.036 0015-0282 http://repositorio.unifesp.br/handle/11600/36661 WOS:000324234900024 |
url |
http://dx.doi.org/10.1016/j.fertnstert.2013.04.036 http://repositorio.unifesp.br/handle/11600/36661 |
identifier_str_mv |
Fertility and Sterility. New York: Elsevier B.V., v. 100, n. 3, p. 667-672, 2013. 10.1016/j.fertnstert.2013.04.036 0015-0282 WOS:000324234900024 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fertility and Sterility |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
dc.format.none.fl_str_mv |
667-672 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268387503636480 |