Phytochemical screening of the dichloromethaneethanolic extract of Eriosema campestre var. macrophylum roots and its antiproliferative effect on human peripheral blood lymphocytes.
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/57614 http://dx.doi.org/10.1016/j.bjp.2015.08.009 |
Resumo: | Eriosema campestre var. macrophylum (Grear) Fortunato, Fabaceae, is a native plant of the Brazilian Cerrado and the decoction of its roots has been used by folk medicine for the therapy of inflammatory diseases. In this study we aimed to investigate the effect of the dichloromethane-ethanolic extract of E. campestre roots on the proliferative response of lymphocytes and to examine the profile of IL-2 production. The effect of dichloromethane-ethanolic extract of E. campestre on the proliferation of phytohemagglutinin-stimulated lymphocytes was evaluated by using flow cytometry and the cell supernatants were assayed for IL-2 concentrations by using an enzyme-linked immunosorbent assay. The phytochemical screening of E. campestre roots was performed to determine the main secondary metabolites through chromogenic and precipitation reactions and by using HPLC-PAD. In addition to the presence of subclasses of flavonoids (flavones and flavonols) in dichloromethaneethanolic extract of E. campestre, we observed that the extract induced a concentration-dependent decrease in IL-2 levels on the supernatant of the cell cultures as well as an antiproliferative effect on T lymphocytes, including CD4+ and CD8+ cells. The anti-inflammatory effects attributed to E. campestre by folk medicine may partly be explained by its antiproliferative action on T lymphocytes. (C) 2016 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda. |
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Santos, Michaelle G.Almeida, Valeria G.Avelar-Freitas, Bethania A.Grael, Cristiane F. F.Gregorio, Luiz E. [UNIFESP]Pereira, Wagner F.Brito-Melo, Gustavo E. A.2020-08-14T13:44:21Z2020-08-14T13:44:21Z2016Revista Brasileira De Farmacognosia-Brazilian Journal Of Pharmacognosy. Curitiba-Pr, v. 26, n. 4, p. 464-470, 2016.0102-695Xhttps://repositorio.unifesp.br/handle/11600/57614http://dx.doi.org/10.1016/j.bjp.2015.08.009WOS000383732900010.pdfS0102-695X201600040046410.1016/j.bjp.2015.08.009WOS:000383732900010Eriosema campestre var. macrophylum (Grear) Fortunato, Fabaceae, is a native plant of the Brazilian Cerrado and the decoction of its roots has been used by folk medicine for the therapy of inflammatory diseases. In this study we aimed to investigate the effect of the dichloromethane-ethanolic extract of E. campestre roots on the proliferative response of lymphocytes and to examine the profile of IL-2 production. The effect of dichloromethane-ethanolic extract of E. campestre on the proliferation of phytohemagglutinin-stimulated lymphocytes was evaluated by using flow cytometry and the cell supernatants were assayed for IL-2 concentrations by using an enzyme-linked immunosorbent assay. The phytochemical screening of E. campestre roots was performed to determine the main secondary metabolites through chromogenic and precipitation reactions and by using HPLC-PAD. In addition to the presence of subclasses of flavonoids (flavones and flavonols) in dichloromethaneethanolic extract of E. campestre, we observed that the extract induced a concentration-dependent decrease in IL-2 levels on the supernatant of the cell cultures as well as an antiproliferative effect on T lymphocytes, including CD4+ and CD8+ cells. The anti-inflammatory effects attributed to E. campestre by folk medicine may partly be explained by its antiproliferative action on T lymphocytes. (C) 2016 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda.FAPEMIGCNPqCAPESUniv Fed Vales Jequitinhonha & Mucuri, Dept Farm, Lab Imunol, Diamantina, MG, BrazilUniv Fed Vales Jequitinhonha & Mucuri, Inst Ciencia & Tecnol, Diamantina, MG, BrazilUniv Fed Vales Jequitinhonha & Mucuri, Lab Farmacognosia, Diamantina, MG, BrazilUniv Fed Sao Paulo, Lab Ciencias Ambientais Quim & Farmaceut, Diadema, SP, BrazilUniv Fed Sao Paulo, Lab Ciencias Ambientais Quim & Farmaceut, Diadema, SP, BrazilFAPEMIG: CBB-APQ-00581-11Web of Science464-470porSoc Brasileira FarmacognosiaRevista Brasileira De Farmacognosia-Brazilian Journal Of PharmacognosyEriosema campestreFlavonoidsCellular proliferationIL-2Phytochemical screening of the dichloromethaneethanolic extract of Eriosema campestre var. macrophylum roots and its antiproliferative effect on human peripheral blood lymphocytes.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCuritiba-Pr264info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000383732900010.pdfapplication/pdf1019784${dspace.ui.url}/bitstream/11600/57614/1/WOS000383732900010.pdf153dc3de752e9c827826872df1660839MD51open accessTEXTWOS000383732900010.pdf.txtWOS000383732900010.pdf.txtExtracted texttext/plain36272${dspace.ui.url}/bitstream/11600/57614/8/WOS000383732900010.pdf.txt4f241434e13b15d2ccf11c46743052caMD58open accessTHUMBNAILWOS000383732900010.pdf.jpgWOS000383732900010.pdf.jpgIM Thumbnailimage/jpeg7465${dspace.ui.url}/bitstream/11600/57614/10/WOS000383732900010.pdf.jpgcbfaa98462f65120db561fdf07f0f11dMD510open access11600/576142023-06-05 19:20:32.87open accessoai:repositorio.unifesp.br:11600/57614Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:20:32Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Phytochemical screening of the dichloromethaneethanolic extract of Eriosema campestre var. macrophylum roots and its antiproliferative effect on human peripheral blood lymphocytes. |
title |
Phytochemical screening of the dichloromethaneethanolic extract of Eriosema campestre var. macrophylum roots and its antiproliferative effect on human peripheral blood lymphocytes. |
spellingShingle |
Phytochemical screening of the dichloromethaneethanolic extract of Eriosema campestre var. macrophylum roots and its antiproliferative effect on human peripheral blood lymphocytes. Santos, Michaelle G. Eriosema campestre Flavonoids Cellular proliferation IL-2 |
title_short |
Phytochemical screening of the dichloromethaneethanolic extract of Eriosema campestre var. macrophylum roots and its antiproliferative effect on human peripheral blood lymphocytes. |
title_full |
Phytochemical screening of the dichloromethaneethanolic extract of Eriosema campestre var. macrophylum roots and its antiproliferative effect on human peripheral blood lymphocytes. |
title_fullStr |
Phytochemical screening of the dichloromethaneethanolic extract of Eriosema campestre var. macrophylum roots and its antiproliferative effect on human peripheral blood lymphocytes. |
title_full_unstemmed |
Phytochemical screening of the dichloromethaneethanolic extract of Eriosema campestre var. macrophylum roots and its antiproliferative effect on human peripheral blood lymphocytes. |
title_sort |
Phytochemical screening of the dichloromethaneethanolic extract of Eriosema campestre var. macrophylum roots and its antiproliferative effect on human peripheral blood lymphocytes. |
author |
Santos, Michaelle G. |
author_facet |
Santos, Michaelle G. Almeida, Valeria G. Avelar-Freitas, Bethania A. Grael, Cristiane F. F. Gregorio, Luiz E. [UNIFESP] Pereira, Wagner F. Brito-Melo, Gustavo E. A. |
author_role |
author |
author2 |
Almeida, Valeria G. Avelar-Freitas, Bethania A. Grael, Cristiane F. F. Gregorio, Luiz E. [UNIFESP] Pereira, Wagner F. Brito-Melo, Gustavo E. A. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Santos, Michaelle G. Almeida, Valeria G. Avelar-Freitas, Bethania A. Grael, Cristiane F. F. Gregorio, Luiz E. [UNIFESP] Pereira, Wagner F. Brito-Melo, Gustavo E. A. |
dc.subject.eng.fl_str_mv |
Eriosema campestre Flavonoids Cellular proliferation IL-2 |
topic |
Eriosema campestre Flavonoids Cellular proliferation IL-2 |
description |
Eriosema campestre var. macrophylum (Grear) Fortunato, Fabaceae, is a native plant of the Brazilian Cerrado and the decoction of its roots has been used by folk medicine for the therapy of inflammatory diseases. In this study we aimed to investigate the effect of the dichloromethane-ethanolic extract of E. campestre roots on the proliferative response of lymphocytes and to examine the profile of IL-2 production. The effect of dichloromethane-ethanolic extract of E. campestre on the proliferation of phytohemagglutinin-stimulated lymphocytes was evaluated by using flow cytometry and the cell supernatants were assayed for IL-2 concentrations by using an enzyme-linked immunosorbent assay. The phytochemical screening of E. campestre roots was performed to determine the main secondary metabolites through chromogenic and precipitation reactions and by using HPLC-PAD. In addition to the presence of subclasses of flavonoids (flavones and flavonols) in dichloromethaneethanolic extract of E. campestre, we observed that the extract induced a concentration-dependent decrease in IL-2 levels on the supernatant of the cell cultures as well as an antiproliferative effect on T lymphocytes, including CD4+ and CD8+ cells. The anti-inflammatory effects attributed to E. campestre by folk medicine may partly be explained by its antiproliferative action on T lymphocytes. (C) 2016 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2020-08-14T13:44:21Z |
dc.date.available.fl_str_mv |
2020-08-14T13:44:21Z |
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info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Revista Brasileira De Farmacognosia-Brazilian Journal Of Pharmacognosy. Curitiba-Pr, v. 26, n. 4, p. 464-470, 2016. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/57614 http://dx.doi.org/10.1016/j.bjp.2015.08.009 |
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0102-695X |
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WOS000383732900010.pdf |
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S0102-695X2016000400464 |
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10.1016/j.bjp.2015.08.009 |
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WOS:000383732900010 |
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Revista Brasileira De Farmacognosia-Brazilian Journal Of Pharmacognosy. Curitiba-Pr, v. 26, n. 4, p. 464-470, 2016. 0102-695X WOS000383732900010.pdf S0102-695X2016000400464 10.1016/j.bjp.2015.08.009 WOS:000383732900010 |
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https://repositorio.unifesp.br/handle/11600/57614 http://dx.doi.org/10.1016/j.bjp.2015.08.009 |
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