Identification of non-tuberculous mycobacteria from the Central Public Health Laboratory from Mato Grosso do Sul and analysis of clinical relevance
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2008 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| Texto Completo: | http://dx.doi.org/10.1590/S1517-83822008000200013 http://repositorio.unifesp.br/handle/11600/4423 |
Resumo: | Non-tuberculous mycobacteria isolated at the Central Public Health Laboratory from Mato Grosso do Sul in 2003 and 2004 were identified by conventional phenotypic methods (TI) and by PCR-Restriction Enzyme Analysis (PRA) using the hsp65 gene as target (PRA-hsp65). With 15 of the 32 analysed isolates, results of both methods were concordant, being 8 Mycobacterium avium, 3 M. fortutium, 1 M. kansasii, 1 M. flavescens, 1 M. peregrinum and 1 Nocardia brasiliensis. TI of 12 isolates was inconclusive. Novel PRA-hsp65 patterns were observed with 11 isolates. Medical data were evaluated for inference of clinical relevance of these isolates. |
| id |
UFSP_6311fcee16b3360d133d9ad5b9643169 |
|---|---|
| oai_identifier_str |
oai:repositorio.unifesp.br/:11600/4423 |
| network_acronym_str |
UFSP |
| network_name_str |
Repositório Institucional da UNIFESP |
| repository_id_str |
3465 |
| spelling |
Identification of non-tuberculous mycobacteria from the Central Public Health Laboratory from Mato Grosso do Sul and analysis of clinical relevanceIdentificação de micobactérias não-tuberculosas do Laboratório Central de Saúde Pública de Mato Grosso de Sul e análise de dados clínicos dos pacientesMycobacteriumPCRIdentificationMicobactériasPCRIdentificaçãoNon-tuberculous mycobacteria isolated at the Central Public Health Laboratory from Mato Grosso do Sul in 2003 and 2004 were identified by conventional phenotypic methods (TI) and by PCR-Restriction Enzyme Analysis (PRA) using the hsp65 gene as target (PRA-hsp65). With 15 of the 32 analysed isolates, results of both methods were concordant, being 8 Mycobacterium avium, 3 M. fortutium, 1 M. kansasii, 1 M. flavescens, 1 M. peregrinum and 1 Nocardia brasiliensis. TI of 12 isolates was inconclusive. Novel PRA-hsp65 patterns were observed with 11 isolates. Medical data were evaluated for inference of clinical relevance of these isolates.Micobactérias não-tuberculosas isoladas no Laboratório Central de Saúde Pública de Mato Grosso do Sul em 2003 e 2004 foram identificadas usando métodos fenotípicos convencionais (TI) e PCR-Restriction Enzyme Analysis (PRA) tendo o gene hsp65 como alvo (PRA-hsp65). Em 15 dos 32 isolados analisados os resultados obtidos com ambos métodos foram concordantes, sendo 8 Mycobacterium avium, 3 M. fortutium, 1 M. kansasii, 1 M. flavescens, 1 M. peregrinum e 1 Nocardia brasiliensis. TI de 12 isolados não foi conclusiva. Perfis não descritos de PRA-hsp65 foram observados com 11 isolados. Dados dos prontuários médicos foram avaliados para inferir a relevância clínica dos isolados.Laboratório Central de Saúde Pública de Mato Grosso do SulInstituto Adolfo Lutz de São PauloUniversidade Federal de Mato Grosso do SulUniversidade Federal de São Paulo (UNIFESP) Departamento de Microbiologia, Imunologia e ParasitologiaUNIFESP, Depto. de Microbiologia, Imunologia e ParasitologiaSciELOSociedade Brasileira de MicrobiologiaLaboratório Central de Saúde Pública de Mato Grosso do SulInstituto Adolfo Lutz de São PauloUniversidade Federal de Mato Grosso do SulUniversidade Federal de São Paulo (UNIFESP)Moraes, Paulo Ricardo de SouzaChimara, Erica [UNIFESP]Telles, Maria Alice da SilvaUeki, Suely Yoko MisukaCunha, Eunice Atsuko TotumiHoner, Michael RobinLeao, Sylvia Cardoso [UNIFESP]2015-06-14T13:38:36Z2015-06-14T13:38:36Z2008-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion268-272application/pdfhttp://dx.doi.org/10.1590/S1517-83822008000200013Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 39, n. 2, p. 268-272, 2008.10.1590/S1517-83822008000200013S1517-83822008000200013.pdf1517-8382S1517-83822008000200013http://repositorio.unifesp.br/handle/11600/4423WOS:000258097100013engBrazilian Journal of Microbiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-06T08:13:24Zoai:repositorio.unifesp.br/:11600/4423Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-06T08:13:24Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
Identification of non-tuberculous mycobacteria from the Central Public Health Laboratory from Mato Grosso do Sul and analysis of clinical relevance Identificação de micobactérias não-tuberculosas do Laboratório Central de Saúde Pública de Mato Grosso de Sul e análise de dados clínicos dos pacientes |
| title |
Identification of non-tuberculous mycobacteria from the Central Public Health Laboratory from Mato Grosso do Sul and analysis of clinical relevance |
| spellingShingle |
Identification of non-tuberculous mycobacteria from the Central Public Health Laboratory from Mato Grosso do Sul and analysis of clinical relevance Moraes, Paulo Ricardo de Souza Mycobacterium PCR Identification Micobactérias PCR Identificação |
| title_short |
Identification of non-tuberculous mycobacteria from the Central Public Health Laboratory from Mato Grosso do Sul and analysis of clinical relevance |
| title_full |
Identification of non-tuberculous mycobacteria from the Central Public Health Laboratory from Mato Grosso do Sul and analysis of clinical relevance |
| title_fullStr |
Identification of non-tuberculous mycobacteria from the Central Public Health Laboratory from Mato Grosso do Sul and analysis of clinical relevance |
| title_full_unstemmed |
Identification of non-tuberculous mycobacteria from the Central Public Health Laboratory from Mato Grosso do Sul and analysis of clinical relevance |
| title_sort |
Identification of non-tuberculous mycobacteria from the Central Public Health Laboratory from Mato Grosso do Sul and analysis of clinical relevance |
| author |
Moraes, Paulo Ricardo de Souza |
| author_facet |
Moraes, Paulo Ricardo de Souza Chimara, Erica [UNIFESP] Telles, Maria Alice da Silva Ueki, Suely Yoko Misuka Cunha, Eunice Atsuko Totumi Honer, Michael Robin Leao, Sylvia Cardoso [UNIFESP] |
| author_role |
author |
| author2 |
Chimara, Erica [UNIFESP] Telles, Maria Alice da Silva Ueki, Suely Yoko Misuka Cunha, Eunice Atsuko Totumi Honer, Michael Robin Leao, Sylvia Cardoso [UNIFESP] |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Laboratório Central de Saúde Pública de Mato Grosso do Sul Instituto Adolfo Lutz de São Paulo Universidade Federal de Mato Grosso do Sul Universidade Federal de São Paulo (UNIFESP) |
| dc.contributor.author.fl_str_mv |
Moraes, Paulo Ricardo de Souza Chimara, Erica [UNIFESP] Telles, Maria Alice da Silva Ueki, Suely Yoko Misuka Cunha, Eunice Atsuko Totumi Honer, Michael Robin Leao, Sylvia Cardoso [UNIFESP] |
| dc.subject.por.fl_str_mv |
Mycobacterium PCR Identification Micobactérias PCR Identificação |
| topic |
Mycobacterium PCR Identification Micobactérias PCR Identificação |
| description |
Non-tuberculous mycobacteria isolated at the Central Public Health Laboratory from Mato Grosso do Sul in 2003 and 2004 were identified by conventional phenotypic methods (TI) and by PCR-Restriction Enzyme Analysis (PRA) using the hsp65 gene as target (PRA-hsp65). With 15 of the 32 analysed isolates, results of both methods were concordant, being 8 Mycobacterium avium, 3 M. fortutium, 1 M. kansasii, 1 M. flavescens, 1 M. peregrinum and 1 Nocardia brasiliensis. TI of 12 isolates was inconclusive. Novel PRA-hsp65 patterns were observed with 11 isolates. Medical data were evaluated for inference of clinical relevance of these isolates. |
| publishDate |
2008 |
| dc.date.none.fl_str_mv |
2008-06-01 2015-06-14T13:38:36Z 2015-06-14T13:38:36Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1517-83822008000200013 Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 39, n. 2, p. 268-272, 2008. 10.1590/S1517-83822008000200013 S1517-83822008000200013.pdf 1517-8382 S1517-83822008000200013 http://repositorio.unifesp.br/handle/11600/4423 WOS:000258097100013 |
| url |
http://dx.doi.org/10.1590/S1517-83822008000200013 http://repositorio.unifesp.br/handle/11600/4423 |
| identifier_str_mv |
Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 39, n. 2, p. 268-272, 2008. 10.1590/S1517-83822008000200013 S1517-83822008000200013.pdf 1517-8382 S1517-83822008000200013 WOS:000258097100013 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Brazilian Journal of Microbiology |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
268-272 application/pdf |
| dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
| publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
| instname_str |
Universidade Federal de São Paulo (UNIFESP) |
| instacron_str |
UNIFESP |
| institution |
UNIFESP |
| reponame_str |
Repositório Institucional da UNIFESP |
| collection |
Repositório Institucional da UNIFESP |
| repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
| repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
| _version_ |
1814268375058087936 |