Discrimination of members of the Mycobacterium avium complex by polymerase chain reaction

Detalhes bibliográficos
Autor(a) principal: Sircili, Marcelo Palma [UNIFESP]
Data de Publicação: 1999
Outros Autores: Roxo, Eliana, Leao, Sylvia Cardoso [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0001-37141999000200011
http://repositorio.unifesp.br/handle/11600/769
Resumo: Mycobacterium avium complex (MAC) species cannot be discriminated by the usual methods of biochemical identification of mycobacteria. This study showed that amplification by PCR of DT1 and DT6, two single copy sequences identified in the genome of M. avium serotype 2, the insertion sequence IS1245, found to be consistently present in M. avium strains and the heat-shock protein gene hsp65, followed by restriction polymorphism analysis, are rapid and accurate tests for the differentiation of the species M. avium, M. intracellulare, and M. scrofulaceum.
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spelling Discrimination of members of the Mycobacterium avium complex by polymerase chain reactionIdentificação molecular de membros do complexo Mycobacterium aviumamplificationPCRMACMycobacteriumidentificationamplificaçãoPCRMACMycobacteriumidentificaçãoMycobacterium avium complex (MAC) species cannot be discriminated by the usual methods of biochemical identification of mycobacteria. This study showed that amplification by PCR of DT1 and DT6, two single copy sequences identified in the genome of M. avium serotype 2, the insertion sequence IS1245, found to be consistently present in M. avium strains and the heat-shock protein gene hsp65, followed by restriction polymorphism analysis, are rapid and accurate tests for the differentiation of the species M. avium, M. intracellulare, and M. scrofulaceum.A identificação bioquímica de micobactérias não permite a discriminação das espécies do complexo Mycobacterium avium (MAC). Este estudo mostrou que a amplificação por PCR de DT1 e DT6, duas sequências de cópia única identificadas no genoma de M. avium sorotipo 2, da sequência de inserção IS1245, encontrada consistentemente em cepas de M. avium e de um fragmento do gene da proteína de choque térmico hsp65, seguida da análise do polimorfismo de restrição, são testes rápidos e acurados para a diferenciação das espécies M. avium, M. intracellulare e M. scrofulaceum.Universidade Federal de São Paulo (UNIFESP)Instituto BiológicoUNIFESPSciELOSociedade Brasileira de MicrobiologiaUniversidade Federal de São Paulo (UNIFESP)Instituto BiológicoSircili, Marcelo Palma [UNIFESP]Roxo, ElianaLeao, Sylvia Cardoso [UNIFESP]2015-06-14T13:24:52Z2015-06-14T13:24:52Z1999-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion144-148application/pdfhttp://dx.doi.org/10.1590/S0001-37141999000200011Revista de Microbiologia. Sociedade Brasileira de Microbiologia, v. 30, n. 2, p. 144-148, 1999.10.1590/S0001-37141999000200011S0001-37141999000200011.pdf0001-3714S0001-37141999000200011http://repositorio.unifesp.br/handle/11600/769engRevista de Microbiologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-05T14:02:36Zoai:repositorio.unifesp.br/:11600/769Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-05T14:02:36Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Discrimination of members of the Mycobacterium avium complex by polymerase chain reaction
Identificação molecular de membros do complexo Mycobacterium avium
title Discrimination of members of the Mycobacterium avium complex by polymerase chain reaction
spellingShingle Discrimination of members of the Mycobacterium avium complex by polymerase chain reaction
Sircili, Marcelo Palma [UNIFESP]
amplification
PCR
MAC
Mycobacterium
identification
amplificação
PCR
MAC
Mycobacterium
identificação
title_short Discrimination of members of the Mycobacterium avium complex by polymerase chain reaction
title_full Discrimination of members of the Mycobacterium avium complex by polymerase chain reaction
title_fullStr Discrimination of members of the Mycobacterium avium complex by polymerase chain reaction
title_full_unstemmed Discrimination of members of the Mycobacterium avium complex by polymerase chain reaction
title_sort Discrimination of members of the Mycobacterium avium complex by polymerase chain reaction
author Sircili, Marcelo Palma [UNIFESP]
author_facet Sircili, Marcelo Palma [UNIFESP]
Roxo, Eliana
Leao, Sylvia Cardoso [UNIFESP]
author_role author
author2 Roxo, Eliana
Leao, Sylvia Cardoso [UNIFESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Instituto Biológico
dc.contributor.author.fl_str_mv Sircili, Marcelo Palma [UNIFESP]
Roxo, Eliana
Leao, Sylvia Cardoso [UNIFESP]
dc.subject.por.fl_str_mv amplification
PCR
MAC
Mycobacterium
identification
amplificação
PCR
MAC
Mycobacterium
identificação
topic amplification
PCR
MAC
Mycobacterium
identification
amplificação
PCR
MAC
Mycobacterium
identificação
description Mycobacterium avium complex (MAC) species cannot be discriminated by the usual methods of biochemical identification of mycobacteria. This study showed that amplification by PCR of DT1 and DT6, two single copy sequences identified in the genome of M. avium serotype 2, the insertion sequence IS1245, found to be consistently present in M. avium strains and the heat-shock protein gene hsp65, followed by restriction polymorphism analysis, are rapid and accurate tests for the differentiation of the species M. avium, M. intracellulare, and M. scrofulaceum.
publishDate 1999
dc.date.none.fl_str_mv 1999-04-01
2015-06-14T13:24:52Z
2015-06-14T13:24:52Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0001-37141999000200011
Revista de Microbiologia. Sociedade Brasileira de Microbiologia, v. 30, n. 2, p. 144-148, 1999.
10.1590/S0001-37141999000200011
S0001-37141999000200011.pdf
0001-3714
S0001-37141999000200011
http://repositorio.unifesp.br/handle/11600/769
url http://dx.doi.org/10.1590/S0001-37141999000200011
http://repositorio.unifesp.br/handle/11600/769
identifier_str_mv Revista de Microbiologia. Sociedade Brasileira de Microbiologia, v. 30, n. 2, p. 144-148, 1999.
10.1590/S0001-37141999000200011
S0001-37141999000200011.pdf
0001-3714
S0001-37141999000200011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista de Microbiologia
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 144-148
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268407640489984

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