Exercise Prevents Diaphragm Wasting Induced by Cigarette Smoke through Modulation of Antioxidant Genes and Metalloproteinases
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/53796 https://dx.doi.org/10.1155/2018/5909053 |
Resumo: | Background. The present study aimed to analyze the effects of physical training on an antioxidant canonical pathway and metalloproteinases activity in diaphragm muscle in a model of cigarette smoke-induced chronic obstructive pulmonary disease (COPD). Methods. Male mice were randomized into control, smoke, exercise, and exercise + smoke groups, which were maintained in trial period of 24 weeks. Gene expression of kelch-like ECH-associated protein 1; nuclear factor erythroid-2 like 2; and heme-oxygenase1 by polymerase chain reaction was performed. Metalloproteinases 2 and 9 activities were analyzed by zymography. Exercise capacity was evaluated by treadmill exercise test before and after the protocol. Results. Aerobic training inhibited diaphragm muscle wasting induced by cigarette smoke exposure. This inhibition was associated with improved aerobic capacity in those animals that were submitted to 24 weeks of aerobic training, when compared to the control and smoke groups, which were not submitted to training. The aerobic training also downregulated the increase of matrix metalloproteinases (MMP-2 and MMP-9) and upregulated antioxidant genes, such as nuclear factor erythroid-2 like 2 (NRF2) and heme-oxygenase1 (HMOX1), in exercise + smoke group compared to smoke group. Conclusions. Treadmill aerobic training protects diaphragm muscle wasting induced by cigarette smoke exposure involving upregulation of antioxidant genes and downregulation of matrix metalloproteinases. |
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Ramos, Gracielle VieiraToledo-Arruda, Alessandra Choqueta de [UNIFESP]Pinheiro-Dardis, Clara MariaSuehiro, Camila LiyokoRusso, Thiago Luiz deVieira, Rodolfo de Paula [UNIFESP]Martins, Milton ArrudaSalvini, Tania FatimaDurigan, Joao Luiz QuagliottiUniversidade Federal de São Paulo (UNIFESP)2020-07-02T18:51:58Z2020-07-02T18:51:58Z2018Biomed Research International. London, v. , p. -, 2018.2314-6133https://repositorio.unifesp.br/handle/11600/53796https://dx.doi.org/10.1155/2018/5909053WOS000428593700001.pdf10.1155/2018/5909053WOS:000428593700001Background. The present study aimed to analyze the effects of physical training on an antioxidant canonical pathway and metalloproteinases activity in diaphragm muscle in a model of cigarette smoke-induced chronic obstructive pulmonary disease (COPD). Methods. Male mice were randomized into control, smoke, exercise, and exercise + smoke groups, which were maintained in trial period of 24 weeks. Gene expression of kelch-like ECH-associated protein 1; nuclear factor erythroid-2 like 2; and heme-oxygenase1 by polymerase chain reaction was performed. Metalloproteinases 2 and 9 activities were analyzed by zymography. Exercise capacity was evaluated by treadmill exercise test before and after the protocol. Results. Aerobic training inhibited diaphragm muscle wasting induced by cigarette smoke exposure. This inhibition was associated with improved aerobic capacity in those animals that were submitted to 24 weeks of aerobic training, when compared to the control and smoke groups, which were not submitted to training. The aerobic training also downregulated the increase of matrix metalloproteinases (MMP-2 and MMP-9) and upregulated antioxidant genes, such as nuclear factor erythroid-2 like 2 (NRF2) and heme-oxygenase1 (HMOX1), in exercise + smoke group compared to smoke group. Conclusions. Treadmill aerobic training protects diaphragm muscle wasting induced by cigarette smoke exposure involving upregulation of antioxidant genes and downregulation of matrix metalloproteinases.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Brasilia, Phys Therapy Div, Brasilia, DF, BrazilUniv Paulista, Dept Phys Therapy, Brasilia, DF, BrazilUniv Sao Paulo, Sch Med, Dept Med LIM 20, Sao Paulo, SP, BrazilFed Univ Sao Paulo UNIFESP, Postgrad Program Sci Human Movement & Rehabil, Av Ana Costa 95, BR-11060001 Santos, SP, BrazilUniv Fed Sao Carlos, Dept Phys Therapy, Sao Carlos, SP, BrazilUniv Brasil, Postgrad Program Bioengn, Rua Carolina Fonseca 235, BR-08230030 Sao Paulo, SP, BrazilBrazilian Inst Teaching & Res Pulm & Exercise Imm, Rua Pedro Ernesto 240, BR-12245520 Sao Jose Dos Campos, SP, BrazilUniv Brasilia, Phys Therapy Div, Brasilia, DF, BrazilFed Univ Sao Paulo UNIFESP, Postgrad Program Sci Human Movement & Rehabil, Av Ana Costa 95, BR-11060001 Santos, SP, BrazilFAPESP: 2012/15165-2Web of Science-engHindawi LtdBiomed Research InternationalExercise Prevents Diaphragm Wasting Induced by Cigarette Smoke through Modulation of Antioxidant Genes and Metalloproteinasesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLondoninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000428593700001.pdfapplication/pdf1706957${dspace.ui.url}/bitstream/11600/53796/1/WOS000428593700001.pdfb6f5714d13487197c049c82419a0c220MD51open accessTEXTWOS000428593700001.pdf.txtWOS000428593700001.pdf.txtExtracted texttext/plain38000${dspace.ui.url}/bitstream/11600/53796/5/WOS000428593700001.pdf.txt499fa055b4d52888738956631ce75820MD55open accessTHUMBNAILWOS000428593700001.pdf.jpgWOS000428593700001.pdf.jpgIM Thumbnailimage/jpeg6377${dspace.ui.url}/bitstream/11600/53796/7/WOS000428593700001.pdf.jpgd49aa33ff909779185ca39dd525bea41MD57open access11600/537962023-06-05 19:15:04.282open accessoai:repositorio.unifesp.br:11600/53796Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:15:04Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Exercise Prevents Diaphragm Wasting Induced by Cigarette Smoke through Modulation of Antioxidant Genes and Metalloproteinases |
title |
Exercise Prevents Diaphragm Wasting Induced by Cigarette Smoke through Modulation of Antioxidant Genes and Metalloproteinases |
spellingShingle |
Exercise Prevents Diaphragm Wasting Induced by Cigarette Smoke through Modulation of Antioxidant Genes and Metalloproteinases Ramos, Gracielle Vieira |
title_short |
Exercise Prevents Diaphragm Wasting Induced by Cigarette Smoke through Modulation of Antioxidant Genes and Metalloproteinases |
title_full |
Exercise Prevents Diaphragm Wasting Induced by Cigarette Smoke through Modulation of Antioxidant Genes and Metalloproteinases |
title_fullStr |
Exercise Prevents Diaphragm Wasting Induced by Cigarette Smoke through Modulation of Antioxidant Genes and Metalloproteinases |
title_full_unstemmed |
Exercise Prevents Diaphragm Wasting Induced by Cigarette Smoke through Modulation of Antioxidant Genes and Metalloproteinases |
title_sort |
Exercise Prevents Diaphragm Wasting Induced by Cigarette Smoke through Modulation of Antioxidant Genes and Metalloproteinases |
author |
Ramos, Gracielle Vieira |
author_facet |
Ramos, Gracielle Vieira Toledo-Arruda, Alessandra Choqueta de [UNIFESP] Pinheiro-Dardis, Clara Maria Suehiro, Camila Liyoko Russo, Thiago Luiz de Vieira, Rodolfo de Paula [UNIFESP] Martins, Milton Arruda Salvini, Tania Fatima Durigan, Joao Luiz Quagliotti |
author_role |
author |
author2 |
Toledo-Arruda, Alessandra Choqueta de [UNIFESP] Pinheiro-Dardis, Clara Maria Suehiro, Camila Liyoko Russo, Thiago Luiz de Vieira, Rodolfo de Paula [UNIFESP] Martins, Milton Arruda Salvini, Tania Fatima Durigan, Joao Luiz Quagliotti |
author2_role |
author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Ramos, Gracielle Vieira Toledo-Arruda, Alessandra Choqueta de [UNIFESP] Pinheiro-Dardis, Clara Maria Suehiro, Camila Liyoko Russo, Thiago Luiz de Vieira, Rodolfo de Paula [UNIFESP] Martins, Milton Arruda Salvini, Tania Fatima Durigan, Joao Luiz Quagliotti |
description |
Background. The present study aimed to analyze the effects of physical training on an antioxidant canonical pathway and metalloproteinases activity in diaphragm muscle in a model of cigarette smoke-induced chronic obstructive pulmonary disease (COPD). Methods. Male mice were randomized into control, smoke, exercise, and exercise + smoke groups, which were maintained in trial period of 24 weeks. Gene expression of kelch-like ECH-associated protein 1; nuclear factor erythroid-2 like 2; and heme-oxygenase1 by polymerase chain reaction was performed. Metalloproteinases 2 and 9 activities were analyzed by zymography. Exercise capacity was evaluated by treadmill exercise test before and after the protocol. Results. Aerobic training inhibited diaphragm muscle wasting induced by cigarette smoke exposure. This inhibition was associated with improved aerobic capacity in those animals that were submitted to 24 weeks of aerobic training, when compared to the control and smoke groups, which were not submitted to training. The aerobic training also downregulated the increase of matrix metalloproteinases (MMP-2 and MMP-9) and upregulated antioxidant genes, such as nuclear factor erythroid-2 like 2 (NRF2) and heme-oxygenase1 (HMOX1), in exercise + smoke group compared to smoke group. Conclusions. Treadmill aerobic training protects diaphragm muscle wasting induced by cigarette smoke exposure involving upregulation of antioxidant genes and downregulation of matrix metalloproteinases. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2020-07-02T18:51:58Z |
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2020-07-02T18:51:58Z |
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article |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Biomed Research International. London, v. , p. -, 2018. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/53796 https://dx.doi.org/10.1155/2018/5909053 |
dc.identifier.issn.none.fl_str_mv |
2314-6133 |
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WOS000428593700001.pdf |
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10.1155/2018/5909053 |
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identifier_str_mv |
Biomed Research International. London, v. , p. -, 2018. 2314-6133 WOS000428593700001.pdf 10.1155/2018/5909053 WOS:000428593700001 |
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https://repositorio.unifesp.br/handle/11600/53796 https://dx.doi.org/10.1155/2018/5909053 |
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