Effects of the topical administration of copaiba oil ointment (Copaifera langsdorffii) in skin flaps viability of rats
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/8038 http://dx.doi.org/10.1590/S0102-86502013001200009 |
Resumo: | PURPOSE: To evaluate the effects of copaiba oil ointment (Copaifera langsdorffii) on dorsal skin flaps in rats. METHODS: Adult male rats (n=30) were distributed into three groups of ten animals each, as follows: GC - control; GCA - absolute control and GT - treated with copaiba ointment. The rats were subjected to dorsal cutaneous skin flap surgery and the animals from the GC and GT received post-operative treatment for eight consecutive days. The animals from the GCA group did not receive treatment while the animals from the GC group received daily topical treatment of ointment without the active ingredient and the animals from the GT group were daily treated with 10% copaiba oil ointment. At the end of each experimental period the lesions were evaluated according to the percentage of necrotic area. Then, fragments from cranial, median and caudal parts were fixed in Boüin's solution and processed for paraffin embedding. The morphology of histological sections (5µm) was evaluated and the number of leucocytes, fibroblasts and blood vessels was also analyzed. The data obtained were submitted to ANOVA test complemented by Tukey-Kramer test (p<0.05). RESULTS: The necrotic area was lower in the group treated with copaiba ointment when compared to the control groups (GCA>GC and GT), while the morphology showed larger granulation tissue with bulky fibroblasts and collagen fibers more arranged in the GT group. The morphometry showed a significant higher number of blood vessels in the median and caudal parts (GT>GCA and GC), leucocytes in the cranial part (GT>GC>GCA), and also fibroblasts in the median (GT and GC> GCA) and caudal parts (GT>GC and GCA) (p<0.05). CONCLUSION: The copaiba oil ointment favors angiogenesis and accelerates the viability of random skin flaps in rats. |
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Estevão, Lígia Reis MouraMedeiros, Juliana Pinto deBaratella-Evêncio, LirianeSimões, Ricardo Santos [UNIFESP]Mendonça, Fábio de SouzaEvêncio-Neto, JoaquimFederal Rural University of Pernambuco Department of Morphology and Animal Physiology Postgraduate Program in Animal BioscienceUFRPE Department of Morphology and Physiology Histology DivisionFederal University of Pernambuco Department of Morphology Histology DivisionUniversidade Federal de São Paulo (UNIFESP)2015-06-14T13:45:41Z2015-06-14T13:45:41Z2013-12-01Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 12, p. 863-869, 2013.0102-8650http://repositorio.unifesp.br/handle/11600/8038http://dx.doi.org/10.1590/S0102-86502013001200009S0102-86502013001200009.pdfS0102-8650201300120000910.1590/S0102-86502013001200009WOS:000328649900009PURPOSE: To evaluate the effects of copaiba oil ointment (Copaifera langsdorffii) on dorsal skin flaps in rats. METHODS: Adult male rats (n=30) were distributed into three groups of ten animals each, as follows: GC - control; GCA - absolute control and GT - treated with copaiba ointment. The rats were subjected to dorsal cutaneous skin flap surgery and the animals from the GC and GT received post-operative treatment for eight consecutive days. The animals from the GCA group did not receive treatment while the animals from the GC group received daily topical treatment of ointment without the active ingredient and the animals from the GT group were daily treated with 10% copaiba oil ointment. At the end of each experimental period the lesions were evaluated according to the percentage of necrotic area. Then, fragments from cranial, median and caudal parts were fixed in Boüin's solution and processed for paraffin embedding. The morphology of histological sections (5µm) was evaluated and the number of leucocytes, fibroblasts and blood vessels was also analyzed. The data obtained were submitted to ANOVA test complemented by Tukey-Kramer test (p<0.05). RESULTS: The necrotic area was lower in the group treated with copaiba ointment when compared to the control groups (GCA>GC and GT), while the morphology showed larger granulation tissue with bulky fibroblasts and collagen fibers more arranged in the GT group. The morphometry showed a significant higher number of blood vessels in the median and caudal parts (GT>GCA and GC), leucocytes in the cranial part (GT>GC>GCA), and also fibroblasts in the median (GT and GC> GCA) and caudal parts (GT>GC and GCA) (p<0.05). CONCLUSION: The copaiba oil ointment favors angiogenesis and accelerates the viability of random skin flaps in rats.Federal Rural University of Pernambuco Department of Morphology and Animal Physiology Postgraduate Program in Animal BioscienceUFRPE Department of Morphology and Physiology Histology DivisionFederal University of Pernambuco Department of Morphology Histology DivisionFederal University of São Paulo Department of Morphology and Genetic Postgraduate Program in MorphologyUNIFESP, Department of Morphology and Genetic Postgraduate Program in MorphologySciELO863-869engSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirurgica BrasileiraSurgical FlapsFabaceaeSkinIschemiaRatsEffects of the topical administration of copaiba oil ointment (Copaifera langsdorffii) in skin flaps viability of ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS0102-86502013001200009.pdfapplication/pdf1515174${dspace.ui.url}/bitstream/11600/8038/1/S0102-86502013001200009.pdf47dccaacc8565ca9a3a8cd1664ed67d0MD51open accessTEXTS0102-86502013001200009.pdf.txtS0102-86502013001200009.pdf.txtExtracted texttext/plain27438${dspace.ui.url}/bitstream/11600/8038/2/S0102-86502013001200009.pdf.txt92785f426608891f5b8eea5ef02d4968MD52open access11600/80382022-02-08 17:45:33.421open accessoai:repositorio.unifesp.br:11600/8038Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-02-08T20:45:33Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Effects of the topical administration of copaiba oil ointment (Copaifera langsdorffii) in skin flaps viability of rats |
title |
Effects of the topical administration of copaiba oil ointment (Copaifera langsdorffii) in skin flaps viability of rats |
spellingShingle |
Effects of the topical administration of copaiba oil ointment (Copaifera langsdorffii) in skin flaps viability of rats Estevão, Lígia Reis Moura Surgical Flaps Fabaceae Skin Ischemia Rats |
title_short |
Effects of the topical administration of copaiba oil ointment (Copaifera langsdorffii) in skin flaps viability of rats |
title_full |
Effects of the topical administration of copaiba oil ointment (Copaifera langsdorffii) in skin flaps viability of rats |
title_fullStr |
Effects of the topical administration of copaiba oil ointment (Copaifera langsdorffii) in skin flaps viability of rats |
title_full_unstemmed |
Effects of the topical administration of copaiba oil ointment (Copaifera langsdorffii) in skin flaps viability of rats |
title_sort |
Effects of the topical administration of copaiba oil ointment (Copaifera langsdorffii) in skin flaps viability of rats |
author |
Estevão, Lígia Reis Moura |
author_facet |
Estevão, Lígia Reis Moura Medeiros, Juliana Pinto de Baratella-Evêncio, Liriane Simões, Ricardo Santos [UNIFESP] Mendonça, Fábio de Souza Evêncio-Neto, Joaquim |
author_role |
author |
author2 |
Medeiros, Juliana Pinto de Baratella-Evêncio, Liriane Simões, Ricardo Santos [UNIFESP] Mendonça, Fábio de Souza Evêncio-Neto, Joaquim |
author2_role |
author author author author author |
dc.contributor.institution.none.fl_str_mv |
Federal Rural University of Pernambuco Department of Morphology and Animal Physiology Postgraduate Program in Animal Bioscience UFRPE Department of Morphology and Physiology Histology Division Federal University of Pernambuco Department of Morphology Histology Division Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Estevão, Lígia Reis Moura Medeiros, Juliana Pinto de Baratella-Evêncio, Liriane Simões, Ricardo Santos [UNIFESP] Mendonça, Fábio de Souza Evêncio-Neto, Joaquim |
dc.subject.eng.fl_str_mv |
Surgical Flaps Fabaceae Skin Ischemia Rats |
topic |
Surgical Flaps Fabaceae Skin Ischemia Rats |
description |
PURPOSE: To evaluate the effects of copaiba oil ointment (Copaifera langsdorffii) on dorsal skin flaps in rats. METHODS: Adult male rats (n=30) were distributed into three groups of ten animals each, as follows: GC - control; GCA - absolute control and GT - treated with copaiba ointment. The rats were subjected to dorsal cutaneous skin flap surgery and the animals from the GC and GT received post-operative treatment for eight consecutive days. The animals from the GCA group did not receive treatment while the animals from the GC group received daily topical treatment of ointment without the active ingredient and the animals from the GT group were daily treated with 10% copaiba oil ointment. At the end of each experimental period the lesions were evaluated according to the percentage of necrotic area. Then, fragments from cranial, median and caudal parts were fixed in Boüin's solution and processed for paraffin embedding. The morphology of histological sections (5µm) was evaluated and the number of leucocytes, fibroblasts and blood vessels was also analyzed. The data obtained were submitted to ANOVA test complemented by Tukey-Kramer test (p<0.05). RESULTS: The necrotic area was lower in the group treated with copaiba ointment when compared to the control groups (GCA>GC and GT), while the morphology showed larger granulation tissue with bulky fibroblasts and collagen fibers more arranged in the GT group. The morphometry showed a significant higher number of blood vessels in the median and caudal parts (GT>GCA and GC), leucocytes in the cranial part (GT>GC>GCA), and also fibroblasts in the median (GT and GC> GCA) and caudal parts (GT>GC and GCA) (p<0.05). CONCLUSION: The copaiba oil ointment favors angiogenesis and accelerates the viability of random skin flaps in rats. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-12-01 |
dc.date.accessioned.fl_str_mv |
2015-06-14T13:45:41Z |
dc.date.available.fl_str_mv |
2015-06-14T13:45:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 12, p. 863-869, 2013. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/8038 http://dx.doi.org/10.1590/S0102-86502013001200009 |
dc.identifier.issn.none.fl_str_mv |
0102-8650 |
dc.identifier.file.none.fl_str_mv |
S0102-86502013001200009.pdf |
dc.identifier.scielo.none.fl_str_mv |
S0102-86502013001200009 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S0102-86502013001200009 |
dc.identifier.wos.none.fl_str_mv |
WOS:000328649900009 |
identifier_str_mv |
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 12, p. 863-869, 2013. 0102-8650 S0102-86502013001200009.pdf S0102-86502013001200009 10.1590/S0102-86502013001200009 WOS:000328649900009 |
url |
http://repositorio.unifesp.br/handle/11600/8038 http://dx.doi.org/10.1590/S0102-86502013001200009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Acta Cirurgica Brasileira |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
863-869 |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
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reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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