Novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of Xanthomonas fuscans subsp aurantifolii
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1186/1471-2164-11-238 http://repositorio.unifesp.br/handle/11600/32469 |
Resumo: | Background: Citrus canker is a disease that has severe economic impact on the citrus industry worldwide. There are three types of canker, called A, B, and C. the three types have different phenotypes and affect different citrus species. the causative agent for type A is Xanthomonas citri subsp. citri, whose genome sequence was made available in 2002. Xanthomonas fuscans subsp. aurantifolii strain B causes canker B and Xanthomonas fuscans subsp. aurantifolii strain C causes canker C.Results: We have sequenced the genomes of strains B and C to draft status. We have compared their genomic content to X. citri subsp. citri and to other Xanthomonas genomes, with special emphasis on type III secreted effector repertoires. in addition to pthA, already known to be present in all three citrus canker strains, two additional effector genes, xopE3 and xopAI, are also present in all three strains and are both located on the same putative genomic island. These two effector genes, along with one other effector-like gene in the same region, are thus good candidates for being pathogenicity factors on citrus. Numerous gene content differences also exist between the three cankers strains, which can be correlated with their different virulence and host range. Particular attention was placed on the analysis of genes involved in biofilm formation and quorum sensing, type IV secretion, flagellum synthesis and motility, lipopolysacharide synthesis, and on the gene xacPNP, which codes for a natriuretic protein.Conclusion: We have uncovered numerous commonalities and differences in gene content between the genomes of the pathogenic agents causing citrus canker A, B, and C and other Xanthomonas genomes. Molecular genetics can now be employed to determine the role of these genes in plant-microbe interactions. the gained knowledge will be instrumental for improving citrus canker control. |
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Novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of Xanthomonas fuscans subsp aurantifoliiBackground: Citrus canker is a disease that has severe economic impact on the citrus industry worldwide. There are three types of canker, called A, B, and C. the three types have different phenotypes and affect different citrus species. the causative agent for type A is Xanthomonas citri subsp. citri, whose genome sequence was made available in 2002. Xanthomonas fuscans subsp. aurantifolii strain B causes canker B and Xanthomonas fuscans subsp. aurantifolii strain C causes canker C.Results: We have sequenced the genomes of strains B and C to draft status. We have compared their genomic content to X. citri subsp. citri and to other Xanthomonas genomes, with special emphasis on type III secreted effector repertoires. in addition to pthA, already known to be present in all three citrus canker strains, two additional effector genes, xopE3 and xopAI, are also present in all three strains and are both located on the same putative genomic island. These two effector genes, along with one other effector-like gene in the same region, are thus good candidates for being pathogenicity factors on citrus. Numerous gene content differences also exist between the three cankers strains, which can be correlated with their different virulence and host range. Particular attention was placed on the analysis of genes involved in biofilm formation and quorum sensing, type IV secretion, flagellum synthesis and motility, lipopolysacharide synthesis, and on the gene xacPNP, which codes for a natriuretic protein.Conclusion: We have uncovered numerous commonalities and differences in gene content between the genomes of the pathogenic agents causing citrus canker A, B, and C and other Xanthomonas genomes. Molecular genetics can now be employed to determine the role of these genes in plant-microbe interactions. the gained knowledge will be instrumental for improving citrus canker control.Virginia Polytech Inst & State Univ, Virginia Bioinformat Inst, Blacksburg, VA 24061 USAUniv Fed Ouro Preto, Inst Ciencias Exatas & Biol, Dept Ciencias Biol, Ouro Preto, MG, BrazilUniv São Paulo, Inst Quim, Dept Bioquim, BR-01498 São Paulo, BrazilUniv Florida, Dept Plant Pathol, Gainesville, FL 32611 USAVirginia Polytech Inst & State Univ, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USAUniv Estadual Paulista, Fac Ciencias Agr & Vet Jaboticabal, Dept Tecnol, Jaboticabal, SP, BrazilUniversidade Federal de São Paulo, Dept Ciencias Biol, São Paulo, BrazilUniv Estadual Paulista, Fac Med Vet & Zootecnia Botucatu, Dept Melhoramento & Nutr Anim, Botucatu, SP, BrazilUniv Estado Santa Catarina, Ctr Ciencias Agrovet, Dept Engn Florestal, Lages, SC, BrazilAllelyx Appl Genom, Campinas, SP, BrazilUniv São Paulo, Escola Super Agr Luiz de Queiroz, Nucleo Apoio Pesquisa Microscopia Eletron Aplicad, Piracicaba, SP, BrazilInst Biol Campinas, Lab Bacteriol Vegetal, Campinas, SP, BrazilUniv São Paulo, Escola Artes Ciencias & Humanidades, São Paulo, BrazilUniv Fed Mato Grosso do Sul, Fac Computacao, Campo Grande, MS, BrazilUniv Estadual Campinas, Inst Computacao, Lab Bioinformat, Campinas, SP, BrazilVirginia Polytech Inst & State Univ, Dept Comp Sci, Blacksburg, VA 24061 USAInst Agron Parana, Londrina, Parana, BrazilUniv Florida, Inst Food & Agr Sci, Mid Florida Res & Educ Ctr, Gainesville, FL 32611 USAUniv Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USAUniversidade Federal de São Paulo, Dept Ciencias Biol, São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)FUNDECITRUSBiomed Central LtdVirginia Polytech Inst & State UnivUniv Fed Ouro PretoUniversidade de São Paulo (USP)Univ FloridaUniv Estadual PaulistaUniversidade Federal de São Paulo (UNIFESP)Univ Estado Santa CatarinaAllelyx Appl GenomInst Biol CampinasUniversidade Federal de Mato Grosso do Sul (UFMS)Universidade Estadual de Campinas (UNICAMP)Inst Agron ParanaUniv Calif BerkeleyMoreira, Leandro M.Almeida, Nalvo F.Potnis, NehaDigiampietri, Luciano A.Adi, Said S.Bortolossi, Julio C.Silva, Ana C. daSilva, Aline M. daMoraes, Fabricio E. deOliveira, Julio C. de [UNIFESP]Souza, Robson F. deFacincani, Agda P.Ferraz, Andre L.Ferro, Maria I.Furlan, Luiz R.Gimenez, Daniele F.Jones, Jeffrey B.Kitajima, Elliot W.Laia, Marcelo L.Leite, Rui P.Nishiyama, Milton Y.Neto, Julio RodriguesNociti, Leticia A.Norman, David J.Ostroski, Eric H.Pereira, Haroldo A.Staskawicz, Brian J.Tezza, Renata I.Ferro, Jesus A.Vinatzer, Boris A.Setubal, Joao Carlos2016-01-24T13:59:35Z2016-01-24T13:59:35Z2010-04-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion25application/pdfhttp://dx.doi.org/10.1186/1471-2164-11-238Bmc Genomics. London: Biomed Central Ltd, v. 11, 25 p., 2010.10.1186/1471-2164-11-238WOS000279860700001.pdf1471-2164http://repositorio.unifesp.br/handle/11600/32469WOS:000279860700001engBmc Genomicsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-30T19:07:02Zoai:repositorio.unifesp.br/:11600/32469Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-30T19:07:02Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of Xanthomonas fuscans subsp aurantifolii |
title |
Novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of Xanthomonas fuscans subsp aurantifolii |
spellingShingle |
Novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of Xanthomonas fuscans subsp aurantifolii Moreira, Leandro M. |
title_short |
Novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of Xanthomonas fuscans subsp aurantifolii |
title_full |
Novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of Xanthomonas fuscans subsp aurantifolii |
title_fullStr |
Novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of Xanthomonas fuscans subsp aurantifolii |
title_full_unstemmed |
Novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of Xanthomonas fuscans subsp aurantifolii |
title_sort |
Novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of Xanthomonas fuscans subsp aurantifolii |
author |
Moreira, Leandro M. |
author_facet |
Moreira, Leandro M. Almeida, Nalvo F. Potnis, Neha Digiampietri, Luciano A. Adi, Said S. Bortolossi, Julio C. Silva, Ana C. da Silva, Aline M. da Moraes, Fabricio E. de Oliveira, Julio C. de [UNIFESP] Souza, Robson F. de Facincani, Agda P. Ferraz, Andre L. Ferro, Maria I. Furlan, Luiz R. Gimenez, Daniele F. Jones, Jeffrey B. Kitajima, Elliot W. Laia, Marcelo L. Leite, Rui P. Nishiyama, Milton Y. Neto, Julio Rodrigues Nociti, Leticia A. Norman, David J. Ostroski, Eric H. Pereira, Haroldo A. Staskawicz, Brian J. Tezza, Renata I. Ferro, Jesus A. Vinatzer, Boris A. Setubal, Joao Carlos |
author_role |
author |
author2 |
Almeida, Nalvo F. Potnis, Neha Digiampietri, Luciano A. Adi, Said S. Bortolossi, Julio C. Silva, Ana C. da Silva, Aline M. da Moraes, Fabricio E. de Oliveira, Julio C. de [UNIFESP] Souza, Robson F. de Facincani, Agda P. Ferraz, Andre L. Ferro, Maria I. Furlan, Luiz R. Gimenez, Daniele F. Jones, Jeffrey B. Kitajima, Elliot W. Laia, Marcelo L. Leite, Rui P. Nishiyama, Milton Y. Neto, Julio Rodrigues Nociti, Leticia A. Norman, David J. Ostroski, Eric H. Pereira, Haroldo A. Staskawicz, Brian J. Tezza, Renata I. Ferro, Jesus A. Vinatzer, Boris A. Setubal, Joao Carlos |
author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Virginia Polytech Inst & State Univ Univ Fed Ouro Preto Universidade de São Paulo (USP) Univ Florida Univ Estadual Paulista Universidade Federal de São Paulo (UNIFESP) Univ Estado Santa Catarina Allelyx Appl Genom Inst Biol Campinas Universidade Federal de Mato Grosso do Sul (UFMS) Universidade Estadual de Campinas (UNICAMP) Inst Agron Parana Univ Calif Berkeley |
dc.contributor.author.fl_str_mv |
Moreira, Leandro M. Almeida, Nalvo F. Potnis, Neha Digiampietri, Luciano A. Adi, Said S. Bortolossi, Julio C. Silva, Ana C. da Silva, Aline M. da Moraes, Fabricio E. de Oliveira, Julio C. de [UNIFESP] Souza, Robson F. de Facincani, Agda P. Ferraz, Andre L. Ferro, Maria I. Furlan, Luiz R. Gimenez, Daniele F. Jones, Jeffrey B. Kitajima, Elliot W. Laia, Marcelo L. Leite, Rui P. Nishiyama, Milton Y. Neto, Julio Rodrigues Nociti, Leticia A. Norman, David J. Ostroski, Eric H. Pereira, Haroldo A. Staskawicz, Brian J. Tezza, Renata I. Ferro, Jesus A. Vinatzer, Boris A. Setubal, Joao Carlos |
description |
Background: Citrus canker is a disease that has severe economic impact on the citrus industry worldwide. There are three types of canker, called A, B, and C. the three types have different phenotypes and affect different citrus species. the causative agent for type A is Xanthomonas citri subsp. citri, whose genome sequence was made available in 2002. Xanthomonas fuscans subsp. aurantifolii strain B causes canker B and Xanthomonas fuscans subsp. aurantifolii strain C causes canker C.Results: We have sequenced the genomes of strains B and C to draft status. We have compared their genomic content to X. citri subsp. citri and to other Xanthomonas genomes, with special emphasis on type III secreted effector repertoires. in addition to pthA, already known to be present in all three citrus canker strains, two additional effector genes, xopE3 and xopAI, are also present in all three strains and are both located on the same putative genomic island. These two effector genes, along with one other effector-like gene in the same region, are thus good candidates for being pathogenicity factors on citrus. Numerous gene content differences also exist between the three cankers strains, which can be correlated with their different virulence and host range. Particular attention was placed on the analysis of genes involved in biofilm formation and quorum sensing, type IV secretion, flagellum synthesis and motility, lipopolysacharide synthesis, and on the gene xacPNP, which codes for a natriuretic protein.Conclusion: We have uncovered numerous commonalities and differences in gene content between the genomes of the pathogenic agents causing citrus canker A, B, and C and other Xanthomonas genomes. Molecular genetics can now be employed to determine the role of these genes in plant-microbe interactions. the gained knowledge will be instrumental for improving citrus canker control. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-04-13 2016-01-24T13:59:35Z 2016-01-24T13:59:35Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1471-2164-11-238 Bmc Genomics. London: Biomed Central Ltd, v. 11, 25 p., 2010. 10.1186/1471-2164-11-238 WOS000279860700001.pdf 1471-2164 http://repositorio.unifesp.br/handle/11600/32469 WOS:000279860700001 |
url |
http://dx.doi.org/10.1186/1471-2164-11-238 http://repositorio.unifesp.br/handle/11600/32469 |
identifier_str_mv |
Bmc Genomics. London: Biomed Central Ltd, v. 11, 25 p., 2010. 10.1186/1471-2164-11-238 WOS000279860700001.pdf 1471-2164 WOS:000279860700001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bmc Genomics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
25 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd |
publisher.none.fl_str_mv |
Biomed Central Ltd |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1814268276762476544 |