HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
dARK ID: | ark:/48912/001300000bkdc |
DOI: | 10.1590/S0100-879X2001000100005 |
Texto Completo: | http://dx.doi.org/10.1590/S0100-879X2001000100005 http://repositorio.unifesp.br/handle/11600/1086 |
Resumo: | In order to assess the molecular epidemiology of HIV-1 in two neighboring cities located near the epicenter of the HIV-1 epidemics in Brazil (Santos and São Paulo), we investigated 83 HIV-1 strains obtained from samples collected in 1995 from intravenous drug users. The V3 through V5 region of the envelope of gp 120 was analyzed by heteroduplex mobility analysis. Of the 95 samples, 12 (12.6%) were PCR negative (6 samples from each group); low DNA concentration was the reason for non-amplification in half of these cases. Of the 42 typed cases from São Paulo, 34 (81%, 95% confidence limits 74.9 to 87.0%) were B and 8 (19%, 95% confidence limits 12.9 to 25.0%) were F, whereas of the 41 typed cases from Santos, 39 (95%, 95% confidence limits 91.6 to 98.4%) were B and 2 (5%, 95% confidence limits 1.6 to 8.4%) were C. We therefore confirm the relationship between clade F and intravenous drug use in São Paulo, and the presence of clade C in Santos. The fact that different genetic subtypes of HIV-1 are co-circulating indicates a need for continuous surveillance for these subtypes as well as for recombinant viruses in Brazil. |
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HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, BrazilHIV-1 subtypesintravenous drug usersmolecular epidemiologyheteroduplex mobility analysisBrazilIn order to assess the molecular epidemiology of HIV-1 in two neighboring cities located near the epicenter of the HIV-1 epidemics in Brazil (Santos and São Paulo), we investigated 83 HIV-1 strains obtained from samples collected in 1995 from intravenous drug users. The V3 through V5 region of the envelope of gp 120 was analyzed by heteroduplex mobility analysis. Of the 95 samples, 12 (12.6%) were PCR negative (6 samples from each group); low DNA concentration was the reason for non-amplification in half of these cases. Of the 42 typed cases from São Paulo, 34 (81%, 95% confidence limits 74.9 to 87.0%) were B and 8 (19%, 95% confidence limits 12.9 to 25.0%) were F, whereas of the 41 typed cases from Santos, 39 (95%, 95% confidence limits 91.6 to 98.4%) were B and 2 (5%, 95% confidence limits 1.6 to 8.4%) were C. We therefore confirm the relationship between clade F and intravenous drug use in São Paulo, and the presence of clade C in Santos. The fact that different genetic subtypes of HIV-1 are co-circulating indicates a need for continuous surveillance for these subtypes as well as for recombinant viruses in Brazil.Instituto Adolfo LutzUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Laboratório de RetrovirologiaFundação Pró-Sangue/Hemocentro de São PauloCentro de Referência de AIDS (CRAIDS)UNIFESP, EPM, Laboratório de RetrovirologiaSciELOAssociação Brasileira de Divulgação CientíficaInstituto Adolfo LutzUniversidade Federal de São Paulo (UNIFESP)Fundação Pró-Sangue/Hemocentro de São PauloCentro de Referência de AIDS (CRAIDS)Rossini, Marília Almeida AntunesDiaz, Ricardo Sobhie [UNIFESP]Caseiro, Marcos MontaniTurcato Junior, Gilberto [UNIFESP]Accetturi, Conceicao Aparecida [UNIFESP]Sabino, Ester Cerdeira [UNIFESP]2015-06-14T13:25:09Z2015-06-14T13:25:09Z2001-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion45-47application/pdfhttp://dx.doi.org/10.1590/S0100-879X2001000100005Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 1, p. 45-47, 2001.10.1590/S0100-879X2001000100005S0100-879X2001000100005.pdf0100-879XS0100-879X2001000100005http://repositorio.unifesp.br/handle/11600/1086WOS:000166675700005ark:/48912/001300000bkdcengBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-29T17:21:10Zoai:repositorio.unifesp.br/:11600/1086Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T20:09:13.820908Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil |
title |
HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil |
spellingShingle |
HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil Rossini, Marília Almeida Antunes HIV-1 subtypes intravenous drug users molecular epidemiology heteroduplex mobility analysis Brazil Rossini, Marília Almeida Antunes HIV-1 subtypes intravenous drug users molecular epidemiology heteroduplex mobility analysis Brazil |
title_short |
HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil |
title_full |
HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil |
title_fullStr |
HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil |
title_full_unstemmed |
HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil |
title_sort |
HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil |
author |
Rossini, Marília Almeida Antunes |
author_facet |
Rossini, Marília Almeida Antunes Rossini, Marília Almeida Antunes Diaz, Ricardo Sobhie [UNIFESP] Caseiro, Marcos Montani Turcato Junior, Gilberto [UNIFESP] Accetturi, Conceicao Aparecida [UNIFESP] Sabino, Ester Cerdeira [UNIFESP] Diaz, Ricardo Sobhie [UNIFESP] Caseiro, Marcos Montani Turcato Junior, Gilberto [UNIFESP] Accetturi, Conceicao Aparecida [UNIFESP] Sabino, Ester Cerdeira [UNIFESP] |
author_role |
author |
author2 |
Diaz, Ricardo Sobhie [UNIFESP] Caseiro, Marcos Montani Turcato Junior, Gilberto [UNIFESP] Accetturi, Conceicao Aparecida [UNIFESP] Sabino, Ester Cerdeira [UNIFESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Instituto Adolfo Lutz Universidade Federal de São Paulo (UNIFESP) Fundação Pró-Sangue/Hemocentro de São Paulo Centro de Referência de AIDS (CRAIDS) |
dc.contributor.author.fl_str_mv |
Rossini, Marília Almeida Antunes Diaz, Ricardo Sobhie [UNIFESP] Caseiro, Marcos Montani Turcato Junior, Gilberto [UNIFESP] Accetturi, Conceicao Aparecida [UNIFESP] Sabino, Ester Cerdeira [UNIFESP] |
dc.subject.por.fl_str_mv |
HIV-1 subtypes intravenous drug users molecular epidemiology heteroduplex mobility analysis Brazil |
topic |
HIV-1 subtypes intravenous drug users molecular epidemiology heteroduplex mobility analysis Brazil |
description |
In order to assess the molecular epidemiology of HIV-1 in two neighboring cities located near the epicenter of the HIV-1 epidemics in Brazil (Santos and São Paulo), we investigated 83 HIV-1 strains obtained from samples collected in 1995 from intravenous drug users. The V3 through V5 region of the envelope of gp 120 was analyzed by heteroduplex mobility analysis. Of the 95 samples, 12 (12.6%) were PCR negative (6 samples from each group); low DNA concentration was the reason for non-amplification in half of these cases. Of the 42 typed cases from São Paulo, 34 (81%, 95% confidence limits 74.9 to 87.0%) were B and 8 (19%, 95% confidence limits 12.9 to 25.0%) were F, whereas of the 41 typed cases from Santos, 39 (95%, 95% confidence limits 91.6 to 98.4%) were B and 2 (5%, 95% confidence limits 1.6 to 8.4%) were C. We therefore confirm the relationship between clade F and intravenous drug use in São Paulo, and the presence of clade C in Santos. The fact that different genetic subtypes of HIV-1 are co-circulating indicates a need for continuous surveillance for these subtypes as well as for recombinant viruses in Brazil. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-01-01 2015-06-14T13:25:09Z 2015-06-14T13:25:09Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-879X2001000100005 Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 1, p. 45-47, 2001. 10.1590/S0100-879X2001000100005 S0100-879X2001000100005.pdf 0100-879X S0100-879X2001000100005 http://repositorio.unifesp.br/handle/11600/1086 WOS:000166675700005 |
dc.identifier.dark.fl_str_mv |
ark:/48912/001300000bkdc |
url |
http://dx.doi.org/10.1590/S0100-879X2001000100005 http://repositorio.unifesp.br/handle/11600/1086 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 1, p. 45-47, 2001. 10.1590/S0100-879X2001000100005 S0100-879X2001000100005.pdf 0100-879X S0100-879X2001000100005 WOS:000166675700005 ark:/48912/001300000bkdc |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
45-47 application/pdf |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1822249616782917632 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S0100-879X2001000100005 |