HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil

Detalhes bibliográficos
Autor(a) principal: Rossini, Marília Almeida Antunes
Data de Publicação: 2001
Outros Autores: Diaz, Ricardo Sobhie [UNIFESP], Caseiro, Marcos Montani, Turcato Junior, Gilberto [UNIFESP], Accetturi, Conceicao Aparecida [UNIFESP], Sabino, Ester Cerdeira [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
dARK ID: ark:/48912/001300000bkdc
DOI: 10.1590/S0100-879X2001000100005
Texto Completo: http://dx.doi.org/10.1590/S0100-879X2001000100005
http://repositorio.unifesp.br/handle/11600/1086
Resumo: In order to assess the molecular epidemiology of HIV-1 in two neighboring cities located near the epicenter of the HIV-1 epidemics in Brazil (Santos and São Paulo), we investigated 83 HIV-1 strains obtained from samples collected in 1995 from intravenous drug users. The V3 through V5 region of the envelope of gp 120 was analyzed by heteroduplex mobility analysis. Of the 95 samples, 12 (12.6%) were PCR negative (6 samples from each group); low DNA concentration was the reason for non-amplification in half of these cases. Of the 42 typed cases from São Paulo, 34 (81%, 95% confidence limits 74.9 to 87.0%) were B and 8 (19%, 95% confidence limits 12.9 to 25.0%) were F, whereas of the 41 typed cases from Santos, 39 (95%, 95% confidence limits 91.6 to 98.4%) were B and 2 (5%, 95% confidence limits 1.6 to 8.4%) were C. We therefore confirm the relationship between clade F and intravenous drug use in São Paulo, and the presence of clade C in Santos. The fact that different genetic subtypes of HIV-1 are co-circulating indicates a need for continuous surveillance for these subtypes as well as for recombinant viruses in Brazil.
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spelling HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, BrazilHIV-1 subtypesintravenous drug usersmolecular epidemiologyheteroduplex mobility analysisBrazilIn order to assess the molecular epidemiology of HIV-1 in two neighboring cities located near the epicenter of the HIV-1 epidemics in Brazil (Santos and São Paulo), we investigated 83 HIV-1 strains obtained from samples collected in 1995 from intravenous drug users. The V3 through V5 region of the envelope of gp 120 was analyzed by heteroduplex mobility analysis. Of the 95 samples, 12 (12.6%) were PCR negative (6 samples from each group); low DNA concentration was the reason for non-amplification in half of these cases. Of the 42 typed cases from São Paulo, 34 (81%, 95% confidence limits 74.9 to 87.0%) were B and 8 (19%, 95% confidence limits 12.9 to 25.0%) were F, whereas of the 41 typed cases from Santos, 39 (95%, 95% confidence limits 91.6 to 98.4%) were B and 2 (5%, 95% confidence limits 1.6 to 8.4%) were C. We therefore confirm the relationship between clade F and intravenous drug use in São Paulo, and the presence of clade C in Santos. The fact that different genetic subtypes of HIV-1 are co-circulating indicates a need for continuous surveillance for these subtypes as well as for recombinant viruses in Brazil.Instituto Adolfo LutzUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Laboratório de RetrovirologiaFundação Pró-Sangue/Hemocentro de São PauloCentro de Referência de AIDS (CRAIDS)UNIFESP, EPM, Laboratório de RetrovirologiaSciELOAssociação Brasileira de Divulgação CientíficaInstituto Adolfo LutzUniversidade Federal de São Paulo (UNIFESP)Fundação Pró-Sangue/Hemocentro de São PauloCentro de Referência de AIDS (CRAIDS)Rossini, Marília Almeida AntunesDiaz, Ricardo Sobhie [UNIFESP]Caseiro, Marcos MontaniTurcato Junior, Gilberto [UNIFESP]Accetturi, Conceicao Aparecida [UNIFESP]Sabino, Ester Cerdeira [UNIFESP]2015-06-14T13:25:09Z2015-06-14T13:25:09Z2001-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion45-47application/pdfhttp://dx.doi.org/10.1590/S0100-879X2001000100005Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 1, p. 45-47, 2001.10.1590/S0100-879X2001000100005S0100-879X2001000100005.pdf0100-879XS0100-879X2001000100005http://repositorio.unifesp.br/handle/11600/1086WOS:000166675700005ark:/48912/001300000bkdcengBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-29T17:21:10Zoai:repositorio.unifesp.br/:11600/1086Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T20:09:13.820908Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil
title HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil
spellingShingle HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil
HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil
Rossini, Marília Almeida Antunes
HIV-1 subtypes
intravenous drug users
molecular epidemiology
heteroduplex mobility analysis
Brazil
Rossini, Marília Almeida Antunes
HIV-1 subtypes
intravenous drug users
molecular epidemiology
heteroduplex mobility analysis
Brazil
title_short HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil
title_full HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil
title_fullStr HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil
HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil
title_full_unstemmed HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil
HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil
title_sort HIV-1 subtypes among intravenous drug users from two neighboring cities in São Paulo State, Brazil
author Rossini, Marília Almeida Antunes
author_facet Rossini, Marília Almeida Antunes
Rossini, Marília Almeida Antunes
Diaz, Ricardo Sobhie [UNIFESP]
Caseiro, Marcos Montani
Turcato Junior, Gilberto [UNIFESP]
Accetturi, Conceicao Aparecida [UNIFESP]
Sabino, Ester Cerdeira [UNIFESP]
Diaz, Ricardo Sobhie [UNIFESP]
Caseiro, Marcos Montani
Turcato Junior, Gilberto [UNIFESP]
Accetturi, Conceicao Aparecida [UNIFESP]
Sabino, Ester Cerdeira [UNIFESP]
author_role author
author2 Diaz, Ricardo Sobhie [UNIFESP]
Caseiro, Marcos Montani
Turcato Junior, Gilberto [UNIFESP]
Accetturi, Conceicao Aparecida [UNIFESP]
Sabino, Ester Cerdeira [UNIFESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto Adolfo Lutz
Universidade Federal de São Paulo (UNIFESP)
Fundação Pró-Sangue/Hemocentro de São Paulo
Centro de Referência de AIDS (CRAIDS)
dc.contributor.author.fl_str_mv Rossini, Marília Almeida Antunes
Diaz, Ricardo Sobhie [UNIFESP]
Caseiro, Marcos Montani
Turcato Junior, Gilberto [UNIFESP]
Accetturi, Conceicao Aparecida [UNIFESP]
Sabino, Ester Cerdeira [UNIFESP]
dc.subject.por.fl_str_mv HIV-1 subtypes
intravenous drug users
molecular epidemiology
heteroduplex mobility analysis
Brazil
topic HIV-1 subtypes
intravenous drug users
molecular epidemiology
heteroduplex mobility analysis
Brazil
description In order to assess the molecular epidemiology of HIV-1 in two neighboring cities located near the epicenter of the HIV-1 epidemics in Brazil (Santos and São Paulo), we investigated 83 HIV-1 strains obtained from samples collected in 1995 from intravenous drug users. The V3 through V5 region of the envelope of gp 120 was analyzed by heteroduplex mobility analysis. Of the 95 samples, 12 (12.6%) were PCR negative (6 samples from each group); low DNA concentration was the reason for non-amplification in half of these cases. Of the 42 typed cases from São Paulo, 34 (81%, 95% confidence limits 74.9 to 87.0%) were B and 8 (19%, 95% confidence limits 12.9 to 25.0%) were F, whereas of the 41 typed cases from Santos, 39 (95%, 95% confidence limits 91.6 to 98.4%) were B and 2 (5%, 95% confidence limits 1.6 to 8.4%) were C. We therefore confirm the relationship between clade F and intravenous drug use in São Paulo, and the presence of clade C in Santos. The fact that different genetic subtypes of HIV-1 are co-circulating indicates a need for continuous surveillance for these subtypes as well as for recombinant viruses in Brazil.
publishDate 2001
dc.date.none.fl_str_mv 2001-01-01
2015-06-14T13:25:09Z
2015-06-14T13:25:09Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-879X2001000100005
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 1, p. 45-47, 2001.
10.1590/S0100-879X2001000100005
S0100-879X2001000100005.pdf
0100-879X
S0100-879X2001000100005
http://repositorio.unifesp.br/handle/11600/1086
WOS:000166675700005
dc.identifier.dark.fl_str_mv ark:/48912/001300000bkdc
url http://dx.doi.org/10.1590/S0100-879X2001000100005
http://repositorio.unifesp.br/handle/11600/1086
identifier_str_mv Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 34, n. 1, p. 45-47, 2001.
10.1590/S0100-879X2001000100005
S0100-879X2001000100005.pdf
0100-879X
S0100-879X2001000100005
WOS:000166675700005
ark:/48912/001300000bkdc
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Medical and Biological Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 45-47
application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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dc.identifier.doi.none.fl_str_mv 10.1590/S0100-879X2001000100005