Beneficial effects of Ginkgo biloba extract on insulin signaling cascade, dyslipidemia, and body adiposity of diet-induced obese rats
Main Author: | |
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Publication Date: | 2014 |
Other Authors: | , , , , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNIFESP |
Download full: | https://repositorio.unifesp.br/handle/11600/8549 https://dx.doi.org/10.1590/1414-431X20142983 |
Summary: | Ginkgo biloba extract (GbE) has been indicated as an efficient medicine for the treatment of diabetes mellitus type 2. It remains unclear if its effects are due to an improvement of the insulin signaling cascade, especially in obese subjects. The aim of the present study was to evaluate the effect of GbE on insulin tolerance, food intake, body adiposity, lipid profile, fasting insulin, and muscle levels of insulin receptor substrate 1 (IRS-1), protein tyrosine phosphatase 1B (PTP-1B), and protein kinase B (Akt), as well as Akt phosphorylation, in diet-induced obese rats. Rats were fed with a high-fat diet (HFD) or a normal fat diet (NFD) for 8 weeks. After that, the HFD group was divided into two groups: rats gavaged with a saline vehicle (HFD+V), and rats gavaged with 500 mg/kg of GbE diluted in the saline vehicle (HFD+Gb). NFD rats were gavaged with the saline vehicle only. At the end of the treatment, the rats were anesthetized, insulin was injected into the portal vein, and after 90s, the gastrocnemius muscle was removed. The quantification of IRS-1, Akt, and Akt phosphorylation was performed using Western blotting. Serum levels of fasting insulin and glucose, triacylglycerols and total cholesterol, and LDL and HDL fractions were measured. An insulin tolerance test was also performed. Ingestion of a hyperlipidic diet promoted loss of insulin sensitivity and also resulted in a significant increase in body adiposity, plasma triacylglycerol, and glucose levels. In addition, GbE treatment significantly reduced food intake and body adiposity while it protected against hyperglycemia and dyslipidemia in diet-induced obesity rats. It also enhanced insulin sensitivity in comparison to HFD+V rats, while it restored insulin-induced Akt phosphorylation, increased IRS-1, and reduced PTP-1B levels in gastrocnemius muscle. The present findings suggest that G. biloba might be efficient in preventing and treating obesity-induced insulin signaling impairment. |
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Banin, Renata Mancini [UNIFESP]Hirata, Bruna Kelly Sousa [UNIFESP]Andrade, Iracema Senna de [UNIFESP]Zemdegs, Juliane Costa Silva [UNIFESP]Clemente, Ana Paula Grotti [UNIFESP]Dornellas, Ana Paula Segantine [UNIFESP]Boldarine, Valter Tadeu [UNIFESP]Estadella, Debora [UNIFESP]Albuquerque, Kelse Tibau de [UNIFESP]Oyama, Lila Missae [UNIFESP]Ribeiro, Eliane Beraldi [UNIFESP]Telles, Monica Marques [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Universidade Federal de Alagoas Faculdade de NutriçãoUniversidade Federal do Rio de Janeiro Curso de Nutrição2015-06-14T13:47:15Z2015-06-14T13:47:15Z2014-09-01Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 47, n. 9, p. 780-788, 2014.0100-879Xhttps://repositorio.unifesp.br/handle/11600/8549https://dx.doi.org/10.1590/1414-431X20142983S0100-879X2014000900780.pdfS0100-879X201400090078010.1590/1414-431X20142983Ginkgo biloba extract (GbE) has been indicated as an efficient medicine for the treatment of diabetes mellitus type 2. It remains unclear if its effects are due to an improvement of the insulin signaling cascade, especially in obese subjects. The aim of the present study was to evaluate the effect of GbE on insulin tolerance, food intake, body adiposity, lipid profile, fasting insulin, and muscle levels of insulin receptor substrate 1 (IRS-1), protein tyrosine phosphatase 1B (PTP-1B), and protein kinase B (Akt), as well as Akt phosphorylation, in diet-induced obese rats. Rats were fed with a high-fat diet (HFD) or a normal fat diet (NFD) for 8 weeks. After that, the HFD group was divided into two groups: rats gavaged with a saline vehicle (HFD+V), and rats gavaged with 500 mg/kg of GbE diluted in the saline vehicle (HFD+Gb). NFD rats were gavaged with the saline vehicle only. At the end of the treatment, the rats were anesthetized, insulin was injected into the portal vein, and after 90s, the gastrocnemius muscle was removed. The quantification of IRS-1, Akt, and Akt phosphorylation was performed using Western blotting. Serum levels of fasting insulin and glucose, triacylglycerols and total cholesterol, and LDL and HDL fractions were measured. An insulin tolerance test was also performed. Ingestion of a hyperlipidic diet promoted loss of insulin sensitivity and also resulted in a significant increase in body adiposity, plasma triacylglycerol, and glucose levels. In addition, GbE treatment significantly reduced food intake and body adiposity while it protected against hyperglycemia and dyslipidemia in diet-induced obesity rats. It also enhanced insulin sensitivity in comparison to HFD+V rats, while it restored insulin-induced Akt phosphorylation, increased IRS-1, and reduced PTP-1B levels in gastrocnemius muscle. The present findings suggest that G. biloba might be efficient in preventing and treating obesity-induced insulin signaling impairment.Universidade Federal de São Paulo (UNIFESP) Departamento de Ciências BiológicasUniversidade Federal de São Paulo (UNIFESP) Departamento de Fisiologia Disciplina de Fisiologia da NutriçãoUniversidade Federal de São Paulo (UNIFESP) Departamento de BiociênciasUniversidade Federal de Alagoas Faculdade de NutriçãoUniversidade Federal do Rio de Janeiro Curso de NutriçãoUNIFESP, Depto. de Ciências BiológicasUNIFESP, Depto. de Fisiologia Disciplina de Fisiologia da NutriçãoUNIFESP, Depto. de BiociênciasSciELO780-788engAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological ResearchGinkgo biloba extractObesityInsulin toleranceBody adiposityAkt phosphorylationRatsBeneficial effects of Ginkgo biloba extract on insulin signaling cascade, dyslipidemia, and body adiposity of diet-induced obese ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS0100-879X2014000900780.pdfapplication/pdf386466${dspace.ui.url}/bitstream/11600/8549/1/S0100-879X2014000900780.pdf07508e1f580a2764226ed880480926f3MD51open accessTEXTS0100-879X2014000900780.pdf.txtS0100-879X2014000900780.pdf.txtExtracted texttext/plain36293${dspace.ui.url}/bitstream/11600/8549/9/S0100-879X2014000900780.pdf.txt5b67f4fc071fbbde2132fccb96eb78f0MD59open accessTHUMBNAILS0100-879X2014000900780.pdf.jpgS0100-879X2014000900780.pdf.jpgIM Thumbnailimage/jpeg6492${dspace.ui.url}/bitstream/11600/8549/11/S0100-879X2014000900780.pdf.jpgf0bcfb352b7d837fee51aed1dff2366fMD511open access11600/85492023-06-05 19:30:22.578open accessoai:repositorio.unifesp.br:11600/8549Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:30:22Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Beneficial effects of Ginkgo biloba extract on insulin signaling cascade, dyslipidemia, and body adiposity of diet-induced obese rats |
title |
Beneficial effects of Ginkgo biloba extract on insulin signaling cascade, dyslipidemia, and body adiposity of diet-induced obese rats |
spellingShingle |
Beneficial effects of Ginkgo biloba extract on insulin signaling cascade, dyslipidemia, and body adiposity of diet-induced obese rats Banin, Renata Mancini [UNIFESP] Ginkgo biloba extract Obesity Insulin tolerance Body adiposity Akt phosphorylation Rats |
title_short |
Beneficial effects of Ginkgo biloba extract on insulin signaling cascade, dyslipidemia, and body adiposity of diet-induced obese rats |
title_full |
Beneficial effects of Ginkgo biloba extract on insulin signaling cascade, dyslipidemia, and body adiposity of diet-induced obese rats |
title_fullStr |
Beneficial effects of Ginkgo biloba extract on insulin signaling cascade, dyslipidemia, and body adiposity of diet-induced obese rats |
title_full_unstemmed |
Beneficial effects of Ginkgo biloba extract on insulin signaling cascade, dyslipidemia, and body adiposity of diet-induced obese rats |
title_sort |
Beneficial effects of Ginkgo biloba extract on insulin signaling cascade, dyslipidemia, and body adiposity of diet-induced obese rats |
author |
Banin, Renata Mancini [UNIFESP] |
author_facet |
Banin, Renata Mancini [UNIFESP] Hirata, Bruna Kelly Sousa [UNIFESP] Andrade, Iracema Senna de [UNIFESP] Zemdegs, Juliane Costa Silva [UNIFESP] Clemente, Ana Paula Grotti [UNIFESP] Dornellas, Ana Paula Segantine [UNIFESP] Boldarine, Valter Tadeu [UNIFESP] Estadella, Debora [UNIFESP] Albuquerque, Kelse Tibau de [UNIFESP] Oyama, Lila Missae [UNIFESP] Ribeiro, Eliane Beraldi [UNIFESP] Telles, Monica Marques [UNIFESP] |
author_role |
author |
author2 |
Hirata, Bruna Kelly Sousa [UNIFESP] Andrade, Iracema Senna de [UNIFESP] Zemdegs, Juliane Costa Silva [UNIFESP] Clemente, Ana Paula Grotti [UNIFESP] Dornellas, Ana Paula Segantine [UNIFESP] Boldarine, Valter Tadeu [UNIFESP] Estadella, Debora [UNIFESP] Albuquerque, Kelse Tibau de [UNIFESP] Oyama, Lila Missae [UNIFESP] Ribeiro, Eliane Beraldi [UNIFESP] Telles, Monica Marques [UNIFESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade Federal de Alagoas Faculdade de Nutrição Universidade Federal do Rio de Janeiro Curso de Nutrição |
dc.contributor.author.fl_str_mv |
Banin, Renata Mancini [UNIFESP] Hirata, Bruna Kelly Sousa [UNIFESP] Andrade, Iracema Senna de [UNIFESP] Zemdegs, Juliane Costa Silva [UNIFESP] Clemente, Ana Paula Grotti [UNIFESP] Dornellas, Ana Paula Segantine [UNIFESP] Boldarine, Valter Tadeu [UNIFESP] Estadella, Debora [UNIFESP] Albuquerque, Kelse Tibau de [UNIFESP] Oyama, Lila Missae [UNIFESP] Ribeiro, Eliane Beraldi [UNIFESP] Telles, Monica Marques [UNIFESP] |
dc.subject.eng.fl_str_mv |
Ginkgo biloba extract Obesity Insulin tolerance Body adiposity Akt phosphorylation Rats |
topic |
Ginkgo biloba extract Obesity Insulin tolerance Body adiposity Akt phosphorylation Rats |
description |
Ginkgo biloba extract (GbE) has been indicated as an efficient medicine for the treatment of diabetes mellitus type 2. It remains unclear if its effects are due to an improvement of the insulin signaling cascade, especially in obese subjects. The aim of the present study was to evaluate the effect of GbE on insulin tolerance, food intake, body adiposity, lipid profile, fasting insulin, and muscle levels of insulin receptor substrate 1 (IRS-1), protein tyrosine phosphatase 1B (PTP-1B), and protein kinase B (Akt), as well as Akt phosphorylation, in diet-induced obese rats. Rats were fed with a high-fat diet (HFD) or a normal fat diet (NFD) for 8 weeks. After that, the HFD group was divided into two groups: rats gavaged with a saline vehicle (HFD+V), and rats gavaged with 500 mg/kg of GbE diluted in the saline vehicle (HFD+Gb). NFD rats were gavaged with the saline vehicle only. At the end of the treatment, the rats were anesthetized, insulin was injected into the portal vein, and after 90s, the gastrocnemius muscle was removed. The quantification of IRS-1, Akt, and Akt phosphorylation was performed using Western blotting. Serum levels of fasting insulin and glucose, triacylglycerols and total cholesterol, and LDL and HDL fractions were measured. An insulin tolerance test was also performed. Ingestion of a hyperlipidic diet promoted loss of insulin sensitivity and also resulted in a significant increase in body adiposity, plasma triacylglycerol, and glucose levels. In addition, GbE treatment significantly reduced food intake and body adiposity while it protected against hyperglycemia and dyslipidemia in diet-induced obesity rats. It also enhanced insulin sensitivity in comparison to HFD+V rats, while it restored insulin-induced Akt phosphorylation, increased IRS-1, and reduced PTP-1B levels in gastrocnemius muscle. The present findings suggest that G. biloba might be efficient in preventing and treating obesity-induced insulin signaling impairment. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-09-01 |
dc.date.accessioned.fl_str_mv |
2015-06-14T13:47:15Z |
dc.date.available.fl_str_mv |
2015-06-14T13:47:15Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 47, n. 9, p. 780-788, 2014. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/8549 https://dx.doi.org/10.1590/1414-431X20142983 |
dc.identifier.issn.none.fl_str_mv |
0100-879X |
dc.identifier.file.none.fl_str_mv |
S0100-879X2014000900780.pdf |
dc.identifier.scielo.none.fl_str_mv |
S0100-879X2014000900780 |
dc.identifier.doi.none.fl_str_mv |
10.1590/1414-431X20142983 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 47, n. 9, p. 780-788, 2014. 0100-879X S0100-879X2014000900780.pdf S0100-879X2014000900780 10.1590/1414-431X20142983 |
url |
https://repositorio.unifesp.br/handle/11600/8549 https://dx.doi.org/10.1590/1414-431X20142983 |
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eng |
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Brazilian Journal of Medical and Biological Research |
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openAccess |
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Associação Brasileira de Divulgação Científica |
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Associação Brasileira de Divulgação Científica |
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