Administration of lopinavir/ritonavir association during rat pregnancy: maternal and fetal effects

Detalhes bibliográficos
Autor(a) principal: Kulay Júnior, Luiz [UNIFESP]
Data de Publicação: 2013
Outros Autores: Hagemann, Cristiane Cadore de Farias [UNIFESP], Nakamura, Mary Uchiyama [UNIFESP], Simoes, Ricardo Santos [UNIFESP], Carvalho, Adelino Moreira de [UNIFESP], Oliveira-Filho, Ricardo Martins [UNIFESP], Espiridião, Silvia [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://www.irog.net/download/?magazine=111
http://repositorio.unifesp.br/handle/11600/43296
Resumo: Purpose: To evaluate the effects of the association of lopinavir and ritonavir administered during the whole period of rat pregnancy. Methods: 62 Wistar rats of the EPM-1 variant weighing about 200 g were randomly divided into five groups: two controls (Ctr1 = stress control, n = 10; and Ctr2 = drug vehicle control, n = 10) and three experimental ones which were treated with an oral solution of lopinavir/ritonavir (Exp1 = 12.8/3.2 mg/kg b.w., n = 14; Exp2 = 38.4/9.6 mg/kg b.w., n = 14; Exp3 = 115.2/28.8 mg/kg b.w., n = 14) from 'day 0' up to the 20th day of pregnancy. Maternal body weight was recorded at the start of the experiment and on the 7th, 14th and 20th day thereafter. At term (20th day), upon laparotomy and hysterotomy, the rats were anesthetized and the amount of implantations, reabsorptions, living fetuses, placentae and intrauterine deaths were recorded. The collected fetuses and placentae were weighed and the concepts were examined under a stereoscope microscope for external malformations. Results: An apparent dose-unrelated lethal effect of the antiviral association on the pregnant rats was observed; notwithstanding, the body weight gain of the surviving rats had no changes, independent of the considered group. It was noted that the quantitative and qualitative intrauterine content of living term rats was indistinguishable from that of the controls. Conclusion: There was some degree of deleterious effects of the administration of the lopinavir/ritonavir association on pregnant rats; such effects eventually led to maternal death. However, neither the surviving rats showed toxicity nor did their concepts present any detectable change which could be related to the drug association.
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spelling Administration of lopinavir/ritonavir association during rat pregnancy: maternal and fetal effectsRatPregnancyLopinavirRitonavirPurpose: To evaluate the effects of the association of lopinavir and ritonavir administered during the whole period of rat pregnancy. Methods: 62 Wistar rats of the EPM-1 variant weighing about 200 g were randomly divided into five groups: two controls (Ctr1 = stress control, n = 10; and Ctr2 = drug vehicle control, n = 10) and three experimental ones which were treated with an oral solution of lopinavir/ritonavir (Exp1 = 12.8/3.2 mg/kg b.w., n = 14; Exp2 = 38.4/9.6 mg/kg b.w., n = 14; Exp3 = 115.2/28.8 mg/kg b.w., n = 14) from 'day 0' up to the 20th day of pregnancy. Maternal body weight was recorded at the start of the experiment and on the 7th, 14th and 20th day thereafter. At term (20th day), upon laparotomy and hysterotomy, the rats were anesthetized and the amount of implantations, reabsorptions, living fetuses, placentae and intrauterine deaths were recorded. The collected fetuses and placentae were weighed and the concepts were examined under a stereoscope microscope for external malformations. Results: An apparent dose-unrelated lethal effect of the antiviral association on the pregnant rats was observed; notwithstanding, the body weight gain of the surviving rats had no changes, independent of the considered group. It was noted that the quantitative and qualitative intrauterine content of living term rats was indistinguishable from that of the controls. Conclusion: There was some degree of deleterious effects of the administration of the lopinavir/ritonavir association on pregnant rats; such effects eventually led to maternal death. However, neither the surviving rats showed toxicity nor did their concepts present any detectable change which could be related to the drug association.Univ Fed Sao Paulo, Dept Obstet & Gynecol, Sch Med UNIFESP EPM, Sao Paulo, BrazilUniv Sao Paulo, Fac Med FMUSP, Sao Paulo, BrazilJose do Rosario Vellano Univ, Sch Med, Dept Internal Med, Alfenas, MG, BrazilSao Paulo Univ USP, Inst Biomed Sci, Dept Pharmacol, Sao Paulo, BrazilABC Med Fdn FUABC, Dept Obstet & Pediat, Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Obstet & Gynecol, Sch Med UNIFESP EPM, Sao Paulo, BrazilWeb of ScienceI R O G Canada, IncUniversidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Jose do Rosario Vellano UnivABC Med Fdn FUABCKulay Júnior, Luiz [UNIFESP]Hagemann, Cristiane Cadore de Farias [UNIFESP]Nakamura, Mary Uchiyama [UNIFESP]Simoes, Ricardo Santos [UNIFESP]Carvalho, Adelino Moreira de [UNIFESP]Oliveira-Filho, Ricardo Martins [UNIFESP]Espiridião, Silvia [UNIFESP]2018-06-15T16:52:39Z2018-06-15T16:52:39Z2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion151-154http://www.irog.net/download/?magazine=111Clinical And Experimental Obstetrics & Gynecology. Montreal: I R O G Canada, Inc, v. 40, n. 1, p. 151-154, 2013.0390-6663http://repositorio.unifesp.br/handle/11600/43296WOS:000315752800040engClinical And Experimental Obstetrics & Gynecologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-05-02T15:58:35Zoai:repositorio.unifesp.br/:11600/43296Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-05-02T15:58:35Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Administration of lopinavir/ritonavir association during rat pregnancy: maternal and fetal effects
title Administration of lopinavir/ritonavir association during rat pregnancy: maternal and fetal effects
spellingShingle Administration of lopinavir/ritonavir association during rat pregnancy: maternal and fetal effects
Kulay Júnior, Luiz [UNIFESP]
Rat
Pregnancy
Lopinavir
Ritonavir
title_short Administration of lopinavir/ritonavir association during rat pregnancy: maternal and fetal effects
title_full Administration of lopinavir/ritonavir association during rat pregnancy: maternal and fetal effects
title_fullStr Administration of lopinavir/ritonavir association during rat pregnancy: maternal and fetal effects
title_full_unstemmed Administration of lopinavir/ritonavir association during rat pregnancy: maternal and fetal effects
title_sort Administration of lopinavir/ritonavir association during rat pregnancy: maternal and fetal effects
author Kulay Júnior, Luiz [UNIFESP]
author_facet Kulay Júnior, Luiz [UNIFESP]
Hagemann, Cristiane Cadore de Farias [UNIFESP]
Nakamura, Mary Uchiyama [UNIFESP]
Simoes, Ricardo Santos [UNIFESP]
Carvalho, Adelino Moreira de [UNIFESP]
Oliveira-Filho, Ricardo Martins [UNIFESP]
Espiridião, Silvia [UNIFESP]
author_role author
author2 Hagemann, Cristiane Cadore de Farias [UNIFESP]
Nakamura, Mary Uchiyama [UNIFESP]
Simoes, Ricardo Santos [UNIFESP]
Carvalho, Adelino Moreira de [UNIFESP]
Oliveira-Filho, Ricardo Martins [UNIFESP]
Espiridião, Silvia [UNIFESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Jose do Rosario Vellano Univ
ABC Med Fdn FUABC
dc.contributor.author.fl_str_mv Kulay Júnior, Luiz [UNIFESP]
Hagemann, Cristiane Cadore de Farias [UNIFESP]
Nakamura, Mary Uchiyama [UNIFESP]
Simoes, Ricardo Santos [UNIFESP]
Carvalho, Adelino Moreira de [UNIFESP]
Oliveira-Filho, Ricardo Martins [UNIFESP]
Espiridião, Silvia [UNIFESP]
dc.subject.por.fl_str_mv Rat
Pregnancy
Lopinavir
Ritonavir
topic Rat
Pregnancy
Lopinavir
Ritonavir
description Purpose: To evaluate the effects of the association of lopinavir and ritonavir administered during the whole period of rat pregnancy. Methods: 62 Wistar rats of the EPM-1 variant weighing about 200 g were randomly divided into five groups: two controls (Ctr1 = stress control, n = 10; and Ctr2 = drug vehicle control, n = 10) and three experimental ones which were treated with an oral solution of lopinavir/ritonavir (Exp1 = 12.8/3.2 mg/kg b.w., n = 14; Exp2 = 38.4/9.6 mg/kg b.w., n = 14; Exp3 = 115.2/28.8 mg/kg b.w., n = 14) from 'day 0' up to the 20th day of pregnancy. Maternal body weight was recorded at the start of the experiment and on the 7th, 14th and 20th day thereafter. At term (20th day), upon laparotomy and hysterotomy, the rats were anesthetized and the amount of implantations, reabsorptions, living fetuses, placentae and intrauterine deaths were recorded. The collected fetuses and placentae were weighed and the concepts were examined under a stereoscope microscope for external malformations. Results: An apparent dose-unrelated lethal effect of the antiviral association on the pregnant rats was observed; notwithstanding, the body weight gain of the surviving rats had no changes, independent of the considered group. It was noted that the quantitative and qualitative intrauterine content of living term rats was indistinguishable from that of the controls. Conclusion: There was some degree of deleterious effects of the administration of the lopinavir/ritonavir association on pregnant rats; such effects eventually led to maternal death. However, neither the surviving rats showed toxicity nor did their concepts present any detectable change which could be related to the drug association.
publishDate 2013
dc.date.none.fl_str_mv 2013-01-01
2018-06-15T16:52:39Z
2018-06-15T16:52:39Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.irog.net/download/?magazine=111
Clinical And Experimental Obstetrics & Gynecology. Montreal: I R O G Canada, Inc, v. 40, n. 1, p. 151-154, 2013.
0390-6663
http://repositorio.unifesp.br/handle/11600/43296
WOS:000315752800040
url http://www.irog.net/download/?magazine=111
http://repositorio.unifesp.br/handle/11600/43296
identifier_str_mv Clinical And Experimental Obstetrics & Gynecology. Montreal: I R O G Canada, Inc, v. 40, n. 1, p. 151-154, 2013.
0390-6663
WOS:000315752800040
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Clinical And Experimental Obstetrics & Gynecology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 151-154
dc.publisher.none.fl_str_mv I R O G Canada, Inc
publisher.none.fl_str_mv I R O G Canada, Inc
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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