Supra-organization and optical anisotropies of the extracellular matrix in the amniotic membrane and limbal stroma before and after explant culture

Detalhes bibliográficos
Autor(a) principal: Valdetaro, Gisele P.
Data de Publicação: 2016
Outros Autores: Aldrovani, Marcela, Padua, Ivan R. M., Cristovam, Priscila Cardoso [UNIFESP], Gomes, José Álvaro Pereira [UNIFESP], Laus, Jose L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1364/BOE.7.004982
https://repositorio.unifesp.br/handle/11600/56620
Resumo: In this research we evaluated the supramolecular organizations and the optical anisotropical properties of the de-epithelialized human amniotic membrane and rabbit limbal stroma, before and after explant culture. Birefringence, monochromatic light spectral absorption and linear dichroism of the main extracellular matrix biopolymers, that is, the fibrillar collagens and proteoglycans, were investigated by polarized light microscopy combined with image analysis. Our results demonstrated that the culture procedure-induced stimuli altered the supra-organizational characteristics (in terms of collagens/proteoglycans spatial orientation and ordered-aggregational state) of the amniotic and limbal extracellular matrix, which led to changes in optical anisotropical properties. (C)2016 Optical Society of America
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spelling Supra-organization and optical anisotropies of the extracellular matrix in the amniotic membrane and limbal stroma before and after explant cultureIn this research we evaluated the supramolecular organizations and the optical anisotropical properties of the de-epithelialized human amniotic membrane and rabbit limbal stroma, before and after explant culture. Birefringence, monochromatic light spectral absorption and linear dichroism of the main extracellular matrix biopolymers, that is, the fibrillar collagens and proteoglycans, were investigated by polarized light microscopy combined with image analysis. Our results demonstrated that the culture procedure-induced stimuli altered the supra-organizational characteristics (in terms of collagens/proteoglycans spatial orientation and ordered-aggregational state) of the amniotic and limbal extracellular matrix, which led to changes in optical anisotropical properties. (C)2016 Optical Society of AmericaUNESP Jaboticabal, Ophthalmol Unit, Dept Small Anim Med & Surg, Fac Agr & Vet Sci, BR-14884900 Jaboticabal, SP, BrazilUniv Fed Sao Paulo, UNIFESP Sao Paulo, Ocular Surface Adv Ctr, BR-04039002 Sao Paulo, SP, BrazilOcular Surface Advanced Center, Universidade Federal de São Paulo (UNIFESP), São Paulo, 04039-002, SP, BrazilWeb of ScienceSao Paulo Research Foundation - FAPESP [2012/17308-5, 2013/01494-7, 2013/25533-1]National Council for Scientific and Technological Development - CNPq [467289/2014-0]Sao Paulo Research Foundation - FAPESP [2012/17308-5, 2013/01494-7, 2013/25533-1]National Council for Scientific and Technological Development - CNPq [467289/2014-0]Optical Soc Amer2020-07-31T12:47:09Z2020-07-31T12:47:09Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion4982-4994application/pdfhttp://dx.doi.org/10.1364/BOE.7.004982Biomedical Optics Express. Washington, v. 7, n. 12, p. 4982-4994, 2016.10.1364/BOE.7.004982WOS000390490700012.pdf2156-7085https://repositorio.unifesp.br/handle/11600/56620WOS:000390490700012engBiomedical Optics ExpressWashingtoninfo:eu-repo/semantics/openAccessValdetaro, Gisele P.Aldrovani, MarcelaPadua, Ivan R. M.Cristovam, Priscila Cardoso [UNIFESP]Gomes, José Álvaro Pereira [UNIFESP]Laus, Jose L.reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-03T10:31:04Zoai:repositorio.unifesp.br/:11600/56620Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-03T10:31:04Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Supra-organization and optical anisotropies of the extracellular matrix in the amniotic membrane and limbal stroma before and after explant culture
title Supra-organization and optical anisotropies of the extracellular matrix in the amniotic membrane and limbal stroma before and after explant culture
spellingShingle Supra-organization and optical anisotropies of the extracellular matrix in the amniotic membrane and limbal stroma before and after explant culture
Valdetaro, Gisele P.
title_short Supra-organization and optical anisotropies of the extracellular matrix in the amniotic membrane and limbal stroma before and after explant culture
title_full Supra-organization and optical anisotropies of the extracellular matrix in the amniotic membrane and limbal stroma before and after explant culture
title_fullStr Supra-organization and optical anisotropies of the extracellular matrix in the amniotic membrane and limbal stroma before and after explant culture
title_full_unstemmed Supra-organization and optical anisotropies of the extracellular matrix in the amniotic membrane and limbal stroma before and after explant culture
title_sort Supra-organization and optical anisotropies of the extracellular matrix in the amniotic membrane and limbal stroma before and after explant culture
author Valdetaro, Gisele P.
author_facet Valdetaro, Gisele P.
Aldrovani, Marcela
Padua, Ivan R. M.
Cristovam, Priscila Cardoso [UNIFESP]
Gomes, José Álvaro Pereira [UNIFESP]
Laus, Jose L.
author_role author
author2 Aldrovani, Marcela
Padua, Ivan R. M.
Cristovam, Priscila Cardoso [UNIFESP]
Gomes, José Álvaro Pereira [UNIFESP]
Laus, Jose L.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Valdetaro, Gisele P.
Aldrovani, Marcela
Padua, Ivan R. M.
Cristovam, Priscila Cardoso [UNIFESP]
Gomes, José Álvaro Pereira [UNIFESP]
Laus, Jose L.
description In this research we evaluated the supramolecular organizations and the optical anisotropical properties of the de-epithelialized human amniotic membrane and rabbit limbal stroma, before and after explant culture. Birefringence, monochromatic light spectral absorption and linear dichroism of the main extracellular matrix biopolymers, that is, the fibrillar collagens and proteoglycans, were investigated by polarized light microscopy combined with image analysis. Our results demonstrated that the culture procedure-induced stimuli altered the supra-organizational characteristics (in terms of collagens/proteoglycans spatial orientation and ordered-aggregational state) of the amniotic and limbal extracellular matrix, which led to changes in optical anisotropical properties. (C)2016 Optical Society of America
publishDate 2016
dc.date.none.fl_str_mv 2016
2020-07-31T12:47:09Z
2020-07-31T12:47:09Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1364/BOE.7.004982
Biomedical Optics Express. Washington, v. 7, n. 12, p. 4982-4994, 2016.
10.1364/BOE.7.004982
WOS000390490700012.pdf
2156-7085
https://repositorio.unifesp.br/handle/11600/56620
WOS:000390490700012
url http://dx.doi.org/10.1364/BOE.7.004982
https://repositorio.unifesp.br/handle/11600/56620
identifier_str_mv Biomedical Optics Express. Washington, v. 7, n. 12, p. 4982-4994, 2016.
10.1364/BOE.7.004982
WOS000390490700012.pdf
2156-7085
WOS:000390490700012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biomedical Optics Express
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 4982-4994
application/pdf
dc.coverage.none.fl_str_mv Washington
dc.publisher.none.fl_str_mv Optical Soc Amer
publisher.none.fl_str_mv Optical Soc Amer
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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