Supra-organization and optical anisotropies of the extracellular matrix in the amniotic membrane and limbal stroma before and after explant culture
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNESP |
| Texto Completo: | http://dx.doi.org/10.1364/BOE.7.004982 http://hdl.handle.net/11449/169213 |
Resumo: | In this research we evaluated the supramolecular organizations and the optical anisotropical properties of the de-epithelialized human amniotic membrane and rabbit limbal stroma, before and after explant culture. Birefringence, monochromatic light spectral absorption and linear dichroism of the main extracellular matrix biopolymers, that is, the fibrillar collagens and proteoglycans, were investigated by polarized light microscopy combined with image analysis. Our results demonstrated that the culture procedure–induced stimuli altered the supra-organizational characteristics (in terms of collagens/proteoglycans spatial orientation and ordered-aggregational state) of the amniotic and limbal extracellular matrix, which led to changes in optical anisotropical properties. |
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Supra-organization and optical anisotropies of the extracellular matrix in the amniotic membrane and limbal stroma before and after explant cultureAnisotropic optical materialsBiology and medicineBirefringenceDichroismOphthalmologyPolarizationIn this research we evaluated the supramolecular organizations and the optical anisotropical properties of the de-epithelialized human amniotic membrane and rabbit limbal stroma, before and after explant culture. Birefringence, monochromatic light spectral absorption and linear dichroism of the main extracellular matrix biopolymers, that is, the fibrillar collagens and proteoglycans, were investigated by polarized light microscopy combined with image analysis. Our results demonstrated that the culture procedure–induced stimuli altered the supra-organizational characteristics (in terms of collagens/proteoglycans spatial orientation and ordered-aggregational state) of the amniotic and limbal extracellular matrix, which led to changes in optical anisotropical properties.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Ophthalmology Unit Department of Small Animal Medicine and Surgery Faculty of Agrarian and Veterinary Sciences UNESPOcular Surface Advanced Center Federal University of S�o Paulo UNIFESPOphthalmology Unit Department of Small Animal Medicine and Surgery Faculty of Agrarian and Veterinary Sciences UNESPFAPESP: 2012/17308-5FAPESP: 2013/01494-7FAPESP: 2013/25533-1CNPq: 467289/2014-0Universidade Estadual Paulista (Unesp)Universidade Federal de São Paulo (UNIFESP)Valdetaro, Gisele P. [UNESP]Aldrovani, Marcela [UNESP]Padua, Ivan R. M. [UNESP]Cristovam, Priscila C.Gomes, Jos� A. P.Laus, Jos� L. [UNESP]2018-12-11T16:44:56Z2018-12-11T16:44:56Z2016-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4982-4994application/pdfhttp://dx.doi.org/10.1364/BOE.7.004982Biomedical Optics Express, v. 7, n. 12, p. 4982-4994, 2016.2156-7085http://hdl.handle.net/11449/16921310.1364/BOE.7.0049822-s2.0-850040104422-s2.0-85004010442.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiomedical Optics Express1,486info:eu-repo/semantics/openAccess2024-01-17T06:27:36Zoai:repositorio.unesp.br:11449/169213Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:16:42.577006Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
| dc.title.none.fl_str_mv |
Supra-organization and optical anisotropies of the extracellular matrix in the amniotic membrane and limbal stroma before and after explant culture |
| title |
Supra-organization and optical anisotropies of the extracellular matrix in the amniotic membrane and limbal stroma before and after explant culture |
| spellingShingle |
Supra-organization and optical anisotropies of the extracellular matrix in the amniotic membrane and limbal stroma before and after explant culture Valdetaro, Gisele P. [UNESP] Anisotropic optical materials Biology and medicine Birefringence Dichroism Ophthalmology Polarization |
| title_short |
Supra-organization and optical anisotropies of the extracellular matrix in the amniotic membrane and limbal stroma before and after explant culture |
| title_full |
Supra-organization and optical anisotropies of the extracellular matrix in the amniotic membrane and limbal stroma before and after explant culture |
| title_fullStr |
Supra-organization and optical anisotropies of the extracellular matrix in the amniotic membrane and limbal stroma before and after explant culture |
| title_full_unstemmed |
Supra-organization and optical anisotropies of the extracellular matrix in the amniotic membrane and limbal stroma before and after explant culture |
| title_sort |
Supra-organization and optical anisotropies of the extracellular matrix in the amniotic membrane and limbal stroma before and after explant culture |
| author |
Valdetaro, Gisele P. [UNESP] |
| author_facet |
Valdetaro, Gisele P. [UNESP] Aldrovani, Marcela [UNESP] Padua, Ivan R. M. [UNESP] Cristovam, Priscila C. Gomes, Jos� A. P. Laus, Jos� L. [UNESP] |
| author_role |
author |
| author2 |
Aldrovani, Marcela [UNESP] Padua, Ivan R. M. [UNESP] Cristovam, Priscila C. Gomes, Jos� A. P. Laus, Jos� L. [UNESP] |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de São Paulo (UNIFESP) |
| dc.contributor.author.fl_str_mv |
Valdetaro, Gisele P. [UNESP] Aldrovani, Marcela [UNESP] Padua, Ivan R. M. [UNESP] Cristovam, Priscila C. Gomes, Jos� A. P. Laus, Jos� L. [UNESP] |
| dc.subject.por.fl_str_mv |
Anisotropic optical materials Biology and medicine Birefringence Dichroism Ophthalmology Polarization |
| topic |
Anisotropic optical materials Biology and medicine Birefringence Dichroism Ophthalmology Polarization |
| description |
In this research we evaluated the supramolecular organizations and the optical anisotropical properties of the de-epithelialized human amniotic membrane and rabbit limbal stroma, before and after explant culture. Birefringence, monochromatic light spectral absorption and linear dichroism of the main extracellular matrix biopolymers, that is, the fibrillar collagens and proteoglycans, were investigated by polarized light microscopy combined with image analysis. Our results demonstrated that the culture procedure–induced stimuli altered the supra-organizational characteristics (in terms of collagens/proteoglycans spatial orientation and ordered-aggregational state) of the amniotic and limbal extracellular matrix, which led to changes in optical anisotropical properties. |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016-12-01 2018-12-11T16:44:56Z 2018-12-11T16:44:56Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1364/BOE.7.004982 Biomedical Optics Express, v. 7, n. 12, p. 4982-4994, 2016. 2156-7085 http://hdl.handle.net/11449/169213 10.1364/BOE.7.004982 2-s2.0-85004010442 2-s2.0-85004010442.pdf |
| url |
http://dx.doi.org/10.1364/BOE.7.004982 http://hdl.handle.net/11449/169213 |
| identifier_str_mv |
Biomedical Optics Express, v. 7, n. 12, p. 4982-4994, 2016. 2156-7085 10.1364/BOE.7.004982 2-s2.0-85004010442 2-s2.0-85004010442.pdf |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Biomedical Optics Express 1,486 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
4982-4994 application/pdf |
| dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
| instname_str |
Universidade Estadual Paulista (UNESP) |
| instacron_str |
UNESP |
| institution |
UNESP |
| reponame_str |
Repositório Institucional da UNESP |
| collection |
Repositório Institucional da UNESP |
| repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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1808129503712509952 |