Neocortical and hippocampal changes after multiple pilocarpine-induced status epilepticus in rats
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/11600/43553 https://dx.doi.org/10.1111/j.1528-1167.2005.31604.x |
Resumo: | Purpose: Multiple episodes of pilocarpine-induced status epilepticus (SE) in developing rats (P7-P9) lead to progressive epileptiform activity and severe cognitive impairment in adulthood. The present work studied possible underlying abnormalities in the neocortex and hippocampus of pilocarpine-treated animals.Methods: Wistar rats were submitted to pilocarpine-induced SE at P7, P8, and P9, and were killed at P35. Immunocytochemistry was performed on 50-mu m vibratome sections, by using antibodies against nonphosphorylated neurofilament (SMI-311), parvalbumin (PV), calbindin (CB), calretinin (CR), and glutamate decarboxylase (GAD-65). Ten-micron cryostat sections were processed for immunohistoblot by using antibodies against GluR1, GluR2/3, and GluR4 alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor subunits and NR2ab N-methyl-D-aspartate (NMDA) receptor subunit.Results: Adult rats submitted to SE at P7-9 showed: (a) altered distribution of neocortical interneurons; (b) increased cortical and reduced hippocampal GAD-65 expression; and (c) altered expression of hippocampal AMPA and NMDA receptors.Conclusions: We conclude that multiple SE episodes during P7-9 generate long-lasting disturbances that underlie behavioral and electrographic abnormalities later in life. |
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Silva, A. V. daRegondi, M. C.Cipelletti, B.Frassoni, C.Cavalheiro, Esper Abrão [UNIFESP]Spreafico, R.Universidade Federal de São Paulo (UNIFESP)Ist Nazl Neurol Carlo Besta2018-06-15T17:17:31Z2018-06-15T17:17:31Z2005-05-01Epilepsia. Hoboken: Wiley-blackwell, v. 46, n. 5, p. 636-642, 2005.0013-9580http://repositorio.unifesp.br/11600/43553https://dx.doi.org/10.1111/j.1528-1167.2005.31604.x10.1111/j.1528-1167.2005.31604.xWOS:000228560000004Purpose: Multiple episodes of pilocarpine-induced status epilepticus (SE) in developing rats (P7-P9) lead to progressive epileptiform activity and severe cognitive impairment in adulthood. The present work studied possible underlying abnormalities in the neocortex and hippocampus of pilocarpine-treated animals.Methods: Wistar rats were submitted to pilocarpine-induced SE at P7, P8, and P9, and were killed at P35. Immunocytochemistry was performed on 50-mu m vibratome sections, by using antibodies against nonphosphorylated neurofilament (SMI-311), parvalbumin (PV), calbindin (CB), calretinin (CR), and glutamate decarboxylase (GAD-65). Ten-micron cryostat sections were processed for immunohistoblot by using antibodies against GluR1, GluR2/3, and GluR4 alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor subunits and NR2ab N-methyl-D-aspartate (NMDA) receptor subunit.Results: Adult rats submitted to SE at P7-9 showed: (a) altered distribution of neocortical interneurons; (b) increased cortical and reduced hippocampal GAD-65 expression; and (c) altered expression of hippocampal AMPA and NMDA receptors.Conclusions: We conclude that multiple SE episodes during P7-9 generate long-lasting disturbances that underlie behavioral and electrographic abnormalities later in life.UNIFESP EPM, Lab Neurol Expt, BR-04023900 Sao Paulo, BrazilIst Nazl Neurol Carlo Besta, Milan, ItalyUNIFESP EPM, Lab Neurol Expt, BR-04023900 Sao Paulo, BrazilWeb of Science636-642engWiley-BlackwellEpilepsiahttp://olabout.wiley.com/WileyCDA/Section/id-406071.htmlinfo:eu-repo/semantics/openAccessimmature brainearly insultimmunocytochemistryimmunohistoblotNeocortical and hippocampal changes after multiple pilocarpine-induced status epilepticus in ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/435532022-02-08 12:10:24.406metadata only accessoai:repositorio.unifesp.br:11600/43553Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-02-08T15:10:24Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Neocortical and hippocampal changes after multiple pilocarpine-induced status epilepticus in rats |
title |
Neocortical and hippocampal changes after multiple pilocarpine-induced status epilepticus in rats |
spellingShingle |
Neocortical and hippocampal changes after multiple pilocarpine-induced status epilepticus in rats Silva, A. V. da immature brain early insult immunocytochemistry immunohistoblot |
title_short |
Neocortical and hippocampal changes after multiple pilocarpine-induced status epilepticus in rats |
title_full |
Neocortical and hippocampal changes after multiple pilocarpine-induced status epilepticus in rats |
title_fullStr |
Neocortical and hippocampal changes after multiple pilocarpine-induced status epilepticus in rats |
title_full_unstemmed |
Neocortical and hippocampal changes after multiple pilocarpine-induced status epilepticus in rats |
title_sort |
Neocortical and hippocampal changes after multiple pilocarpine-induced status epilepticus in rats |
author |
Silva, A. V. da |
author_facet |
Silva, A. V. da Regondi, M. C. Cipelletti, B. Frassoni, C. Cavalheiro, Esper Abrão [UNIFESP] Spreafico, R. |
author_role |
author |
author2 |
Regondi, M. C. Cipelletti, B. Frassoni, C. Cavalheiro, Esper Abrão [UNIFESP] Spreafico, R. |
author2_role |
author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Ist Nazl Neurol Carlo Besta |
dc.contributor.author.fl_str_mv |
Silva, A. V. da Regondi, M. C. Cipelletti, B. Frassoni, C. Cavalheiro, Esper Abrão [UNIFESP] Spreafico, R. |
dc.subject.eng.fl_str_mv |
immature brain early insult immunocytochemistry immunohistoblot |
topic |
immature brain early insult immunocytochemistry immunohistoblot |
description |
Purpose: Multiple episodes of pilocarpine-induced status epilepticus (SE) in developing rats (P7-P9) lead to progressive epileptiform activity and severe cognitive impairment in adulthood. The present work studied possible underlying abnormalities in the neocortex and hippocampus of pilocarpine-treated animals.Methods: Wistar rats were submitted to pilocarpine-induced SE at P7, P8, and P9, and were killed at P35. Immunocytochemistry was performed on 50-mu m vibratome sections, by using antibodies against nonphosphorylated neurofilament (SMI-311), parvalbumin (PV), calbindin (CB), calretinin (CR), and glutamate decarboxylase (GAD-65). Ten-micron cryostat sections were processed for immunohistoblot by using antibodies against GluR1, GluR2/3, and GluR4 alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor subunits and NR2ab N-methyl-D-aspartate (NMDA) receptor subunit.Results: Adult rats submitted to SE at P7-9 showed: (a) altered distribution of neocortical interneurons; (b) increased cortical and reduced hippocampal GAD-65 expression; and (c) altered expression of hippocampal AMPA and NMDA receptors.Conclusions: We conclude that multiple SE episodes during P7-9 generate long-lasting disturbances that underlie behavioral and electrographic abnormalities later in life. |
publishDate |
2005 |
dc.date.issued.fl_str_mv |
2005-05-01 |
dc.date.accessioned.fl_str_mv |
2018-06-15T17:17:31Z |
dc.date.available.fl_str_mv |
2018-06-15T17:17:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Epilepsia. Hoboken: Wiley-blackwell, v. 46, n. 5, p. 636-642, 2005. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/11600/43553 https://dx.doi.org/10.1111/j.1528-1167.2005.31604.x |
dc.identifier.issn.none.fl_str_mv |
0013-9580 |
dc.identifier.doi.none.fl_str_mv |
10.1111/j.1528-1167.2005.31604.x |
dc.identifier.wos.none.fl_str_mv |
WOS:000228560000004 |
identifier_str_mv |
Epilepsia. Hoboken: Wiley-blackwell, v. 46, n. 5, p. 636-642, 2005. 0013-9580 10.1111/j.1528-1167.2005.31604.x WOS:000228560000004 |
url |
http://repositorio.unifesp.br/11600/43553 https://dx.doi.org/10.1111/j.1528-1167.2005.31604.x |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Epilepsia |
dc.rights.driver.fl_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
636-642 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764173306757120 |