Evaluation of reagent strips for ascitic fluid leukocyte determination: is it a possible alternative for spontaneous bacterial peritonitis rapid diagnosis?

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Tarsila Campanha da Rocha [UNIFESP]
Data de Publicação: 2007
Outros Autores: Kondo, Mario [UNIFESP], Feldner, Ana Cristina de Castro Amaral [UNIFESP], Parise, Edison Roberto [UNIFESP], Bragagnolo Júnior, Maurício Augusto [UNIFESP], Souza, Aécio Flávio Meirelles de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S1413-86702007000100017
http://repositorio.unifesp.br/handle/11600/3539
Resumo: In order to evaluate the accuracy of a urine reagent dipstick (Multistix 10SG®) to determine ascitic fluid leukocyte count, we prospectively studied 106 cirrhotic patients from April 2003 to December 2004, in two different centers (Federal University of São Paulo - UNIFESP-EPM and Federal University of Juiz de Fora - HU-UFJF) for the rapid bedside diagnosis of spontaneous bacterial peritonitis. The mean age 54 ± 12 years, there was a predominance of males (eighty-two patients, 77%), and alcohol was the most frequent etiology (43%). Forty-four percent of patients were classified as Child B and fifty-one as Child C (51%). Abdominal paracentesis was performed both in outpatient and inpatient settings and the Multistix 10SG® was tested. Eleven cases of spontaneous bacterial peritonitis were identified by means of polymorphonuclear count. If we considered the positive Multistix 10SG® result of 3 or more, the sensitivity, specificity, positive and negative predictive value were respectively 71%, 99%, 91% and 98%. With a positive reagent strip result taken as grade 2 (traces) or more, sensitivity was 86% and specificity was 96% with positive and negative predictive values of 60% and 99%, respectively. Diagnostic accuracy was 95%. We concluded that the use of a urine reagent dipstick (Multistix 10SG®) could be considered a quick, easy and cheap method for ascitic fluid cellularity determination in SBP diagnosis.
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spelling Evaluation of reagent strips for ascitic fluid leukocyte determination: is it a possible alternative for spontaneous bacterial peritonitis rapid diagnosis?Ascitic fluidrapid diagnosisdipstickbacterial peritonitisIn order to evaluate the accuracy of a urine reagent dipstick (Multistix 10SG®) to determine ascitic fluid leukocyte count, we prospectively studied 106 cirrhotic patients from April 2003 to December 2004, in two different centers (Federal University of São Paulo - UNIFESP-EPM and Federal University of Juiz de Fora - HU-UFJF) for the rapid bedside diagnosis of spontaneous bacterial peritonitis. The mean age 54 ± 12 years, there was a predominance of males (eighty-two patients, 77%), and alcohol was the most frequent etiology (43%). Forty-four percent of patients were classified as Child B and fifty-one as Child C (51%). Abdominal paracentesis was performed both in outpatient and inpatient settings and the Multistix 10SG® was tested. Eleven cases of spontaneous bacterial peritonitis were identified by means of polymorphonuclear count. If we considered the positive Multistix 10SG® result of 3 or more, the sensitivity, specificity, positive and negative predictive value were respectively 71%, 99%, 91% and 98%. With a positive reagent strip result taken as grade 2 (traces) or more, sensitivity was 86% and specificity was 96% with positive and negative predictive values of 60% and 99%, respectively. Diagnostic accuracy was 95%. We concluded that the use of a urine reagent dipstick (Multistix 10SG®) could be considered a quick, easy and cheap method for ascitic fluid cellularity determination in SBP diagnosis.Federal University of São Paulo São Paulo Medical SchoolFederal University of Juiz de Fora HUUNIFESP, EPMSciELOBrazilian Society of Infectious DiseasesUniversidade Federal de São Paulo (UNIFESP)Federal University of Juiz de Fora HURibeiro, Tarsila Campanha da Rocha [UNIFESP]Kondo, Mario [UNIFESP]Feldner, Ana Cristina de Castro Amaral [UNIFESP]Parise, Edison Roberto [UNIFESP]Bragagnolo Júnior, Maurício Augusto [UNIFESP]Souza, Aécio Flávio Meirelles de2015-06-14T13:36:43Z2015-06-14T13:36:43Z2007-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion70-74lapplication/pdfhttp://dx.doi.org/10.1590/S1413-86702007000100017Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 11, n. 1, p. 70-74l, 2007.10.1590/S1413-86702007000100017S1413-86702007000100017.pdf1413-8670S1413-86702007000100017http://repositorio.unifesp.br/handle/11600/3539WOS:000253997600017engBrazilian Journal of Infectious Diseasesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-29T23:58:44Zoai:repositorio.unifesp.br/:11600/3539Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-29T23:58:44Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Evaluation of reagent strips for ascitic fluid leukocyte determination: is it a possible alternative for spontaneous bacterial peritonitis rapid diagnosis?
title Evaluation of reagent strips for ascitic fluid leukocyte determination: is it a possible alternative for spontaneous bacterial peritonitis rapid diagnosis?
spellingShingle Evaluation of reagent strips for ascitic fluid leukocyte determination: is it a possible alternative for spontaneous bacterial peritonitis rapid diagnosis?
Ribeiro, Tarsila Campanha da Rocha [UNIFESP]
Ascitic fluid
rapid diagnosis
dipstick
bacterial peritonitis
title_short Evaluation of reagent strips for ascitic fluid leukocyte determination: is it a possible alternative for spontaneous bacterial peritonitis rapid diagnosis?
title_full Evaluation of reagent strips for ascitic fluid leukocyte determination: is it a possible alternative for spontaneous bacterial peritonitis rapid diagnosis?
title_fullStr Evaluation of reagent strips for ascitic fluid leukocyte determination: is it a possible alternative for spontaneous bacterial peritonitis rapid diagnosis?
title_full_unstemmed Evaluation of reagent strips for ascitic fluid leukocyte determination: is it a possible alternative for spontaneous bacterial peritonitis rapid diagnosis?
title_sort Evaluation of reagent strips for ascitic fluid leukocyte determination: is it a possible alternative for spontaneous bacterial peritonitis rapid diagnosis?
author Ribeiro, Tarsila Campanha da Rocha [UNIFESP]
author_facet Ribeiro, Tarsila Campanha da Rocha [UNIFESP]
Kondo, Mario [UNIFESP]
Feldner, Ana Cristina de Castro Amaral [UNIFESP]
Parise, Edison Roberto [UNIFESP]
Bragagnolo Júnior, Maurício Augusto [UNIFESP]
Souza, Aécio Flávio Meirelles de
author_role author
author2 Kondo, Mario [UNIFESP]
Feldner, Ana Cristina de Castro Amaral [UNIFESP]
Parise, Edison Roberto [UNIFESP]
Bragagnolo Júnior, Maurício Augusto [UNIFESP]
Souza, Aécio Flávio Meirelles de
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Federal University of Juiz de Fora HU
dc.contributor.author.fl_str_mv Ribeiro, Tarsila Campanha da Rocha [UNIFESP]
Kondo, Mario [UNIFESP]
Feldner, Ana Cristina de Castro Amaral [UNIFESP]
Parise, Edison Roberto [UNIFESP]
Bragagnolo Júnior, Maurício Augusto [UNIFESP]
Souza, Aécio Flávio Meirelles de
dc.subject.por.fl_str_mv Ascitic fluid
rapid diagnosis
dipstick
bacterial peritonitis
topic Ascitic fluid
rapid diagnosis
dipstick
bacterial peritonitis
description In order to evaluate the accuracy of a urine reagent dipstick (Multistix 10SG®) to determine ascitic fluid leukocyte count, we prospectively studied 106 cirrhotic patients from April 2003 to December 2004, in two different centers (Federal University of São Paulo - UNIFESP-EPM and Federal University of Juiz de Fora - HU-UFJF) for the rapid bedside diagnosis of spontaneous bacterial peritonitis. The mean age 54 ± 12 years, there was a predominance of males (eighty-two patients, 77%), and alcohol was the most frequent etiology (43%). Forty-four percent of patients were classified as Child B and fifty-one as Child C (51%). Abdominal paracentesis was performed both in outpatient and inpatient settings and the Multistix 10SG® was tested. Eleven cases of spontaneous bacterial peritonitis were identified by means of polymorphonuclear count. If we considered the positive Multistix 10SG® result of 3 or more, the sensitivity, specificity, positive and negative predictive value were respectively 71%, 99%, 91% and 98%. With a positive reagent strip result taken as grade 2 (traces) or more, sensitivity was 86% and specificity was 96% with positive and negative predictive values of 60% and 99%, respectively. Diagnostic accuracy was 95%. We concluded that the use of a urine reagent dipstick (Multistix 10SG®) could be considered a quick, easy and cheap method for ascitic fluid cellularity determination in SBP diagnosis.
publishDate 2007
dc.date.none.fl_str_mv 2007-02-01
2015-06-14T13:36:43Z
2015-06-14T13:36:43Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1413-86702007000100017
Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 11, n. 1, p. 70-74l, 2007.
10.1590/S1413-86702007000100017
S1413-86702007000100017.pdf
1413-8670
S1413-86702007000100017
http://repositorio.unifesp.br/handle/11600/3539
WOS:000253997600017
url http://dx.doi.org/10.1590/S1413-86702007000100017
http://repositorio.unifesp.br/handle/11600/3539
identifier_str_mv Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 11, n. 1, p. 70-74l, 2007.
10.1590/S1413-86702007000100017
S1413-86702007000100017.pdf
1413-8670
S1413-86702007000100017
WOS:000253997600017
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Infectious Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 70-74l
application/pdf
dc.publisher.none.fl_str_mv Brazilian Society of Infectious Diseases
publisher.none.fl_str_mv Brazilian Society of Infectious Diseases
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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