Cross Talk between Peritoneal Macrophages and B-1 Cells in Vitro

• Autor(a) principal: Thies, Felipe Garutti [UNIFESP]
• Data de Publicação: 2013
• Outros Autores: Laurindo, Maria Fernanda Lucatelli [UNIFESP], Perez, Elizabeth Cristina [UNIFESP], Novaes e Brito, Ronni Romulo [UNIFESP], Mariano, Mario [UNIFESP], Popi, Ana Flavia [UNIFESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://repositorio.unifesp.br/handle/11600/36336; http://dx.doi.org/10.1371/journal.pone.0062805

B-1 cells constitute a distinct B cell population with unique phenotypic and functional characteristics. They represent the main B cell population found in mouse peritoneal and pleural cavities. the communication between B-1 cells and peritoneal macrophages has been previously studied, and the effect this interaction has on macrophages has been previously described. Using an in vitro co-culture model, herein we demonstrated that peritoneal macrophages were able to increase survival rates and to stimulate proliferation of B-1 cells. IL-6 was also found to be important in B-1 cell survival; recombinant IL-6 increases the percentage of viable B-1 cells in culture. Furthermore, molecules involved in the IL-6 signaling pathway, such as STAT-3 and Bcl-2, were highly expressed in B-1 cells after co-culture with peritoneal macrophages. IL-6-deficient peritoneal macrophages were not able to increase B-1 cell survival, confirming the importance of this cytokine. Altogether, our results indicate a novel mechanism in which peritoneal macrophages are able to regulate the B-1 population via IL-6 secretion.