Cross Talk between Peritoneal Macrophages and B-1 Cells in Vitro
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/36336 http://dx.doi.org/10.1371/journal.pone.0062805 |
Resumo: | B-1 cells constitute a distinct B cell population with unique phenotypic and functional characteristics. They represent the main B cell population found in mouse peritoneal and pleural cavities. the communication between B-1 cells and peritoneal macrophages has been previously studied, and the effect this interaction has on macrophages has been previously described. Using an in vitro co-culture model, herein we demonstrated that peritoneal macrophages were able to increase survival rates and to stimulate proliferation of B-1 cells. IL-6 was also found to be important in B-1 cell survival; recombinant IL-6 increases the percentage of viable B-1 cells in culture. Furthermore, molecules involved in the IL-6 signaling pathway, such as STAT-3 and Bcl-2, were highly expressed in B-1 cells after co-culture with peritoneal macrophages. IL-6-deficient peritoneal macrophages were not able to increase B-1 cell survival, confirming the importance of this cytokine. Altogether, our results indicate a novel mechanism in which peritoneal macrophages are able to regulate the B-1 population via IL-6 secretion. |
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Thies, Felipe Garutti [UNIFESP]Laurindo, Maria Fernanda Lucatelli [UNIFESP]Perez, Elizabeth Cristina [UNIFESP]Novaes e Brito, Ronni Romulo [UNIFESP]Mariano, Mario [UNIFESP]Popi, Ana Flavia [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Univ Paulista UNIP2016-01-24T14:31:45Z2016-01-24T14:31:45Z2013-05-08Plos One. San Francisco: Public Library Science, v. 8, n. 5, 11 p., 2013.1932-6203http://repositorio.unifesp.br/handle/11600/36336http://dx.doi.org/10.1371/journal.pone.0062805WOS000319055600045.pdf10.1371/journal.pone.0062805WOS:000319055600045B-1 cells constitute a distinct B cell population with unique phenotypic and functional characteristics. They represent the main B cell population found in mouse peritoneal and pleural cavities. the communication between B-1 cells and peritoneal macrophages has been previously studied, and the effect this interaction has on macrophages has been previously described. Using an in vitro co-culture model, herein we demonstrated that peritoneal macrophages were able to increase survival rates and to stimulate proliferation of B-1 cells. IL-6 was also found to be important in B-1 cell survival; recombinant IL-6 increases the percentage of viable B-1 cells in culture. Furthermore, molecules involved in the IL-6 signaling pathway, such as STAT-3 and Bcl-2, were highly expressed in B-1 cells after co-culture with peritoneal macrophages. IL-6-deficient peritoneal macrophages were not able to increase B-1 cell survival, confirming the importance of this cytokine. Altogether, our results indicate a novel mechanism in which peritoneal macrophages are able to regulate the B-1 population via IL-6 secretion.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, Discipline Immunol, São Paulo, BrazilUniv Paulista UNIP, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, Discipline Immunol, São Paulo, BrazilFAPESP: 2008/58561-0FAPESP: 2011/50256-6Web of Science11engPublic Library SciencePlos OneCross Talk between Peritoneal Macrophages and B-1 Cells in Vitroinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000319055600045.pdfapplication/pdf1227988${dspace.ui.url}/bitstream/11600/36336/1/WOS000319055600045.pdffffa37a2034a4191e16e0b3545d92e9fMD51open accessTEXTWOS000319055600045.pdf.txtWOS000319055600045.pdf.txtExtracted texttext/plain44337${dspace.ui.url}/bitstream/11600/36336/2/WOS000319055600045.pdf.txt9ea4868ad4c94dba974507e3877f9a7cMD52open access11600/363362022-06-02 09:20:35.4open accessoai:repositorio.unifesp.br:11600/36336Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-06-02T12:20:35Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Cross Talk between Peritoneal Macrophages and B-1 Cells in Vitro |
title |
Cross Talk between Peritoneal Macrophages and B-1 Cells in Vitro |
spellingShingle |
Cross Talk between Peritoneal Macrophages and B-1 Cells in Vitro Thies, Felipe Garutti [UNIFESP] |
title_short |
Cross Talk between Peritoneal Macrophages and B-1 Cells in Vitro |
title_full |
Cross Talk between Peritoneal Macrophages and B-1 Cells in Vitro |
title_fullStr |
Cross Talk between Peritoneal Macrophages and B-1 Cells in Vitro |
title_full_unstemmed |
Cross Talk between Peritoneal Macrophages and B-1 Cells in Vitro |
title_sort |
Cross Talk between Peritoneal Macrophages and B-1 Cells in Vitro |
author |
Thies, Felipe Garutti [UNIFESP] |
author_facet |
Thies, Felipe Garutti [UNIFESP] Laurindo, Maria Fernanda Lucatelli [UNIFESP] Perez, Elizabeth Cristina [UNIFESP] Novaes e Brito, Ronni Romulo [UNIFESP] Mariano, Mario [UNIFESP] Popi, Ana Flavia [UNIFESP] |
author_role |
author |
author2 |
Laurindo, Maria Fernanda Lucatelli [UNIFESP] Perez, Elizabeth Cristina [UNIFESP] Novaes e Brito, Ronni Romulo [UNIFESP] Mariano, Mario [UNIFESP] Popi, Ana Flavia [UNIFESP] |
author2_role |
author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Univ Paulista UNIP |
dc.contributor.author.fl_str_mv |
Thies, Felipe Garutti [UNIFESP] Laurindo, Maria Fernanda Lucatelli [UNIFESP] Perez, Elizabeth Cristina [UNIFESP] Novaes e Brito, Ronni Romulo [UNIFESP] Mariano, Mario [UNIFESP] Popi, Ana Flavia [UNIFESP] |
description |
B-1 cells constitute a distinct B cell population with unique phenotypic and functional characteristics. They represent the main B cell population found in mouse peritoneal and pleural cavities. the communication between B-1 cells and peritoneal macrophages has been previously studied, and the effect this interaction has on macrophages has been previously described. Using an in vitro co-culture model, herein we demonstrated that peritoneal macrophages were able to increase survival rates and to stimulate proliferation of B-1 cells. IL-6 was also found to be important in B-1 cell survival; recombinant IL-6 increases the percentage of viable B-1 cells in culture. Furthermore, molecules involved in the IL-6 signaling pathway, such as STAT-3 and Bcl-2, were highly expressed in B-1 cells after co-culture with peritoneal macrophages. IL-6-deficient peritoneal macrophages were not able to increase B-1 cell survival, confirming the importance of this cytokine. Altogether, our results indicate a novel mechanism in which peritoneal macrophages are able to regulate the B-1 population via IL-6 secretion. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-05-08 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:31:45Z |
dc.date.available.fl_str_mv |
2016-01-24T14:31:45Z |
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info:eu-repo/semantics/publishedVersion |
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article |
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dc.identifier.citation.fl_str_mv |
Plos One. San Francisco: Public Library Science, v. 8, n. 5, 11 p., 2013. |
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http://repositorio.unifesp.br/handle/11600/36336 http://dx.doi.org/10.1371/journal.pone.0062805 |
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1932-6203 |
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WOS000319055600045.pdf |
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10.1371/journal.pone.0062805 |
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WOS:000319055600045 |
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Plos One. San Francisco: Public Library Science, v. 8, n. 5, 11 p., 2013. 1932-6203 WOS000319055600045.pdf 10.1371/journal.pone.0062805 WOS:000319055600045 |
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http://repositorio.unifesp.br/handle/11600/36336 http://dx.doi.org/10.1371/journal.pone.0062805 |
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Public Library Science |
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Public Library Science |
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