First-time Isolation of Flavonoids and Cytotoxic Potential of the Amazonian Shrub Ptychopetalum olacoides Benth

• Autor(a) principal: Dutra, Karen Danielle Borba
• Data de Publicação: 2017
• Outros Autores: Macedo, Arthur Ladeira, Montenegro, Raquel Carvalho, Jimenez, Paula Christine [UNIFESP], Castro, Rosane Nora, Epifânio, Rosangela de Almeida, Vasconcelos, Thatyana Rocha Alves, Valverde, Alessandra Leda
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: https://dx.doi.org/10.21577/1984-6835.20170137; https://repositorio.unifesp.br/handle/11600/55280
• topic: Antitumor; Cancer; Flavonoids; Olacaceae; P. olacoides

In the present study, three flavonoids, 3-O-methylquercetin (1), 3,4'-O-dimethylquercetin (2) and 3,7-O-dimethylquercetin (3) were isolated and characterized for the first time from a methanol extract obtained from the species Ptychopetalum olacoides. The structures of compounds were identified by spectroscopic methods (1D-, 2D-NMR, MS and UV) and confirmed by comparison with the respective literature data. The cytotoxic effect of crude extract was evaluated in vitro against three human cancer cell lines. The results showed a mild cytotoxic activity (IC50 = 45.16 mu g/mL) against breast cancer (MCF-7). However, crude extract did not exhibit any cytotoxic effect against normal cell human fibroblast (MRC-5).