Comparative EPR and fluorescence conformational studies of fully active spin-labeled melanotropic peptides
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2001 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| dARK ID: | ark:/48912/0013000015b0k |
| Texto Completo: | https://dx.doi.org/10.1016/S0014-5793(01)02449-8 https://repositorio.unifesp.br/handle/11600/26557 |
| Resumo: | Similar to melanocyte stimulating hormone (alpha -MSH), its potent and long-acting analogue, [Nle(4), D-Phe(7)]alpha -MSH, when labeled with the paramagnetic amino acid probe 2,2,6,6-tetramethylpiperidine-N-oxyl-4-amino-4-carboxylic acid (Toac), maintains its full biological potency, thus validating any comparative structural investigations between the two labeled peptides, Correlation times, calculated from tire electron paramagnetic resonance signal of Toac bound to the peptides, and Toac-Trp distances, estimated from the Toac fluorescence quenching of the Trp residue present in the peptides, indicate a more rigid and folded structure for the potent analogue as compared to the hormone, in aqueous medium. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies. |
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Comparative EPR and fluorescence conformational studies of fully active spin-labeled melanotropic peptidesPeptideAlpha-melanocyte stimulating hormoneElectron paramagnetic resonance2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acidFluorescenceSpin labelSimilar to melanocyte stimulating hormone (alpha -MSH), its potent and long-acting analogue, [Nle(4), D-Phe(7)]alpha -MSH, when labeled with the paramagnetic amino acid probe 2,2,6,6-tetramethylpiperidine-N-oxyl-4-amino-4-carboxylic acid (Toac), maintains its full biological potency, thus validating any comparative structural investigations between the two labeled peptides, Correlation times, calculated from tire electron paramagnetic resonance signal of Toac bound to the peptides, and Toac-Trp distances, estimated from the Toac fluorescence quenching of the Trp residue present in the peptides, indicate a more rigid and folded structure for the potent analogue as compared to the hormone, in aqueous medium. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.Universidade Federal de São Paulo, Dept Biofis, BR-04044020 São Paulo, BrazilUniv São Paulo, Inst Fis, BR-66318 São Paulo, BrazilUniv São Paulo, Inst Biociencias, Dept Fisiol, BR-11176 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biofis, BR-04044020 São Paulo, BrazilWeb of ScienceElsevier B.V.Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Nakaie, Clovis Ryuichi [UNIFESP]Barbosa, Simone Reis [UNIFESP]Vieira, Renata de Freitas Fischer [UNIFESP]Fernandez, Roberto MoratoCilli, Eduardo Maffud [UNIFESP]Castrucci, Ana Maria de LauroVisconti, Maria AparecidaIto, Amando SiuitiLamy, Maria Teresa2016-01-24T12:31:23Z2016-01-24T12:31:23Z2001-05-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion103-107application/pdfhttps://dx.doi.org/10.1016/S0014-5793(01)02449-8Febs Letters. Amsterdam: Elsevier B.V., v. 497, n. 2-3, p. 103-107, 2001.10.1016/S0014-5793(01)02449-8WOS000169120900009.pdf0014-5793https://repositorio.unifesp.br/handle/11600/26557WOS:000169120900009ark:/48912/0013000015b0kengFebs Lettersinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-07T07:25:15Zoai:repositorio.unifesp.br/:11600/26557Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-07T07:25:15Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
Comparative EPR and fluorescence conformational studies of fully active spin-labeled melanotropic peptides |
| title |
Comparative EPR and fluorescence conformational studies of fully active spin-labeled melanotropic peptides |
| spellingShingle |
Comparative EPR and fluorescence conformational studies of fully active spin-labeled melanotropic peptides Nakaie, Clovis Ryuichi [UNIFESP] Peptide Alpha-melanocyte stimulating hormone Electron paramagnetic resonance 2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid Fluorescence Spin label |
| title_short |
Comparative EPR and fluorescence conformational studies of fully active spin-labeled melanotropic peptides |
| title_full |
Comparative EPR and fluorescence conformational studies of fully active spin-labeled melanotropic peptides |
| title_fullStr |
Comparative EPR and fluorescence conformational studies of fully active spin-labeled melanotropic peptides |
| title_full_unstemmed |
Comparative EPR and fluorescence conformational studies of fully active spin-labeled melanotropic peptides |
| title_sort |
Comparative EPR and fluorescence conformational studies of fully active spin-labeled melanotropic peptides |
| author |
Nakaie, Clovis Ryuichi [UNIFESP] |
| author_facet |
Nakaie, Clovis Ryuichi [UNIFESP] Barbosa, Simone Reis [UNIFESP] Vieira, Renata de Freitas Fischer [UNIFESP] Fernandez, Roberto Morato Cilli, Eduardo Maffud [UNIFESP] Castrucci, Ana Maria de Lauro Visconti, Maria Aparecida Ito, Amando Siuiti Lamy, Maria Teresa |
| author_role |
author |
| author2 |
Barbosa, Simone Reis [UNIFESP] Vieira, Renata de Freitas Fischer [UNIFESP] Fernandez, Roberto Morato Cilli, Eduardo Maffud [UNIFESP] Castrucci, Ana Maria de Lauro Visconti, Maria Aparecida Ito, Amando Siuiti Lamy, Maria Teresa |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) |
| dc.contributor.author.fl_str_mv |
Nakaie, Clovis Ryuichi [UNIFESP] Barbosa, Simone Reis [UNIFESP] Vieira, Renata de Freitas Fischer [UNIFESP] Fernandez, Roberto Morato Cilli, Eduardo Maffud [UNIFESP] Castrucci, Ana Maria de Lauro Visconti, Maria Aparecida Ito, Amando Siuiti Lamy, Maria Teresa |
| dc.subject.por.fl_str_mv |
Peptide Alpha-melanocyte stimulating hormone Electron paramagnetic resonance 2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid Fluorescence Spin label |
| topic |
Peptide Alpha-melanocyte stimulating hormone Electron paramagnetic resonance 2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid Fluorescence Spin label |
| description |
Similar to melanocyte stimulating hormone (alpha -MSH), its potent and long-acting analogue, [Nle(4), D-Phe(7)]alpha -MSH, when labeled with the paramagnetic amino acid probe 2,2,6,6-tetramethylpiperidine-N-oxyl-4-amino-4-carboxylic acid (Toac), maintains its full biological potency, thus validating any comparative structural investigations between the two labeled peptides, Correlation times, calculated from tire electron paramagnetic resonance signal of Toac bound to the peptides, and Toac-Trp distances, estimated from the Toac fluorescence quenching of the Trp residue present in the peptides, indicate a more rigid and folded structure for the potent analogue as compared to the hormone, in aqueous medium. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies. |
| publishDate |
2001 |
| dc.date.none.fl_str_mv |
2001-05-25 2016-01-24T12:31:23Z 2016-01-24T12:31:23Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://dx.doi.org/10.1016/S0014-5793(01)02449-8 Febs Letters. Amsterdam: Elsevier B.V., v. 497, n. 2-3, p. 103-107, 2001. 10.1016/S0014-5793(01)02449-8 WOS000169120900009.pdf 0014-5793 https://repositorio.unifesp.br/handle/11600/26557 WOS:000169120900009 |
| dc.identifier.dark.fl_str_mv |
ark:/48912/0013000015b0k |
| url |
https://dx.doi.org/10.1016/S0014-5793(01)02449-8 https://repositorio.unifesp.br/handle/11600/26557 |
| identifier_str_mv |
Febs Letters. Amsterdam: Elsevier B.V., v. 497, n. 2-3, p. 103-107, 2001. 10.1016/S0014-5793(01)02449-8 WOS000169120900009.pdf 0014-5793 WOS:000169120900009 ark:/48912/0013000015b0k |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Febs Letters |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
| eu_rights_str_mv |
openAccess |
| rights_invalid_str_mv |
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
| dc.format.none.fl_str_mv |
103-107 application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier B.V. |
| publisher.none.fl_str_mv |
Elsevier B.V. |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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Universidade Federal de São Paulo (UNIFESP) |
| instacron_str |
UNIFESP |
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UNIFESP |
| reponame_str |
Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP |
| repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
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biblioteca.csp@unifesp.br |
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1828484764050391040 |
