First synthesis of a fully active spin-labeled peptide hormone

Detalhes bibliográficos
Autor(a) principal: Barbosa, Simone Reis [UNIFESP
Data de Publicação: 1999
Outros Autores: Cilli, Eduardo Maffud [UNIFESP], Lamy-Freund, M. T., Castrucci, AML, Nakaie, Clovis Ryuichi [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1016/S0014-5793(99)00172-6
http://repositorio.unifesp.br/handle/11600/26045
Resumo: For the first time in the electron spin resonance (ESR) and peptide synthesis fields, a fully active spin-labeled peptide hormone was reported. the ESR spectra of this alpha-melanocyte stimulating hormone (alpha-MSH) analogue (acetyl-Toac(0)-alpha-MSH) where Toac is the paramagnetic amino acid probe 2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid, suggested a pH-independent conformation and a more restricted movement comparatively to the free Toac, Owing to its equivalent biological potency in a skin pigmentation assay as compared to the native alpha-MSH and its unique characteristic (paramagnetic, naturally fluorescent and fully active), this analogue is of great potential for investigation of relevant physiological roles reported for a-MSH, (C) 1999 Federation of European Biochemical Societies.
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spelling First synthesis of a fully active spin-labeled peptide hormonespin-labeled peptidealpha-melanocyte stimulating hormoneelectron spin resonanceskin pigmentationFor the first time in the electron spin resonance (ESR) and peptide synthesis fields, a fully active spin-labeled peptide hormone was reported. the ESR spectra of this alpha-melanocyte stimulating hormone (alpha-MSH) analogue (acetyl-Toac(0)-alpha-MSH) where Toac is the paramagnetic amino acid probe 2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid, suggested a pH-independent conformation and a more restricted movement comparatively to the free Toac, Owing to its equivalent biological potency in a skin pigmentation assay as compared to the native alpha-MSH and its unique characteristic (paramagnetic, naturally fluorescent and fully active), this analogue is of great potential for investigation of relevant physiological roles reported for a-MSH, (C) 1999 Federation of European Biochemical Societies.Universidade Federal de São Paulo, Dept Biofis, BR-04044020 São Paulo, BrazilUniv São Paulo, Inst Fis, BR-01498 São Paulo, BrazilUniv São Paulo, Inst Biociencias, Dept Fisiol, BR-01498 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biofis, BR-04044020 São Paulo, BrazilWeb of ScienceElsevier B.V.Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Barbosa, Simone Reis [UNIFESPCilli, Eduardo Maffud [UNIFESP]Lamy-Freund, M. T.Castrucci, AMLNakaie, Clovis Ryuichi [UNIFESP]2016-01-24T12:30:46Z2016-01-24T12:30:46Z1999-03-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion45-48application/pdfhttp://dx.doi.org/10.1016/S0014-5793(99)00172-6Febs Letters. Amsterdam: Elsevier B.V., v. 446, n. 1, p. 45-48, 1999.10.1016/S0014-5793(99)00172-6WOS000079123100011.pdf0014-5793http://repositorio.unifesp.br/handle/11600/26045WOS:000079123100011engFebs Lettersinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-30T20:47:31Zoai:repositorio.unifesp.br/:11600/26045Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-30T20:47:31Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv First synthesis of a fully active spin-labeled peptide hormone
title First synthesis of a fully active spin-labeled peptide hormone
spellingShingle First synthesis of a fully active spin-labeled peptide hormone
Barbosa, Simone Reis [UNIFESP
spin-labeled peptide
alpha-melanocyte stimulating hormone
electron spin resonance
skin pigmentation
title_short First synthesis of a fully active spin-labeled peptide hormone
title_full First synthesis of a fully active spin-labeled peptide hormone
title_fullStr First synthesis of a fully active spin-labeled peptide hormone
title_full_unstemmed First synthesis of a fully active spin-labeled peptide hormone
title_sort First synthesis of a fully active spin-labeled peptide hormone
author Barbosa, Simone Reis [UNIFESP
author_facet Barbosa, Simone Reis [UNIFESP
Cilli, Eduardo Maffud [UNIFESP]
Lamy-Freund, M. T.
Castrucci, AML
Nakaie, Clovis Ryuichi [UNIFESP]
author_role author
author2 Cilli, Eduardo Maffud [UNIFESP]
Lamy-Freund, M. T.
Castrucci, AML
Nakaie, Clovis Ryuichi [UNIFESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Barbosa, Simone Reis [UNIFESP
Cilli, Eduardo Maffud [UNIFESP]
Lamy-Freund, M. T.
Castrucci, AML
Nakaie, Clovis Ryuichi [UNIFESP]
dc.subject.por.fl_str_mv spin-labeled peptide
alpha-melanocyte stimulating hormone
electron spin resonance
skin pigmentation
topic spin-labeled peptide
alpha-melanocyte stimulating hormone
electron spin resonance
skin pigmentation
description For the first time in the electron spin resonance (ESR) and peptide synthesis fields, a fully active spin-labeled peptide hormone was reported. the ESR spectra of this alpha-melanocyte stimulating hormone (alpha-MSH) analogue (acetyl-Toac(0)-alpha-MSH) where Toac is the paramagnetic amino acid probe 2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid, suggested a pH-independent conformation and a more restricted movement comparatively to the free Toac, Owing to its equivalent biological potency in a skin pigmentation assay as compared to the native alpha-MSH and its unique characteristic (paramagnetic, naturally fluorescent and fully active), this analogue is of great potential for investigation of relevant physiological roles reported for a-MSH, (C) 1999 Federation of European Biochemical Societies.
publishDate 1999
dc.date.none.fl_str_mv 1999-03-05
2016-01-24T12:30:46Z
2016-01-24T12:30:46Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/S0014-5793(99)00172-6
Febs Letters. Amsterdam: Elsevier B.V., v. 446, n. 1, p. 45-48, 1999.
10.1016/S0014-5793(99)00172-6
WOS000079123100011.pdf
0014-5793
http://repositorio.unifesp.br/handle/11600/26045
WOS:000079123100011
url http://dx.doi.org/10.1016/S0014-5793(99)00172-6
http://repositorio.unifesp.br/handle/11600/26045
identifier_str_mv Febs Letters. Amsterdam: Elsevier B.V., v. 446, n. 1, p. 45-48, 1999.
10.1016/S0014-5793(99)00172-6
WOS000079123100011.pdf
0014-5793
WOS:000079123100011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Febs Letters
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.format.none.fl_str_mv 45-48
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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