Small bowel transplantation in outbred rats

Detalhes bibliográficos
Autor(a) principal: Waisberg, Daniel Reis
Data de Publicação: 2011
Outros Autores: Lee, André Dong Wong, Santos, Rafael Miyashiro Nunes dos, Mory, Eduardo Kenji, Costa, Anderson Lino, Montero, Edna Frasson de Souza [UNIFESP], Chaib, Eleazar, D'albuquerque, Luis Augusto Carneiro, Galvao, Flavio Henrique Ferreira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0102-86502011000600015
http://repositorio.unifesp.br/handle/11600/6792
Resumo: PURPOSE: To investigate the clinical evolution of orthotopic small bowel transplantation in outbred rats. METHODS: Seventy-two outbred Wistar rats weighting from 250 to 300g were used as donor and recipient in 36 consecutives ortothopic small intestine transplantation without immunosuppression. The graft was transplanted into the recipient using end-to-side aortic and portacaval microvascular anastomosis. Procedure duration, animal clinical course and survival were evaluated. Survival shorter than four days was considered technical failure. Recipients were sacrificed with signs of severe graft rejection or survival longer than 120 days. Necropsies were performed in all recipients to access histopathological changes in the graft. RESULTS: Median time for the procedure was 107 minutes. Six recipients (16.7%) presented technical failure. Twenty-seven recipients were sacrificed due to rejection, being nineteen (52.7%) between 7th and 15th postoperative day and eight (22.2%) between 34th and 47th postoperative day. Graft histology confirmed severe acute cellular rejection in those recipients. Uneventful evolution and survival longer than 120 days without rejection were observed in three recipients (8.3%). CONCLUSION: Intestinal transplantation in outbred rats without immunosuppressant regiment accomplishes variable clinical evolution.
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spelling Small bowel transplantation in outbred ratsTransplante de intestino delgado em ratos não-isogênicosIntestineSmallTransplantationMicrosurgeryAnimalsOutbred StrainsRatsIntestino DelgadoTransplanteMicrocirurgiaAnimais não EndogâmicosRatosPURPOSE: To investigate the clinical evolution of orthotopic small bowel transplantation in outbred rats. METHODS: Seventy-two outbred Wistar rats weighting from 250 to 300g were used as donor and recipient in 36 consecutives ortothopic small intestine transplantation without immunosuppression. The graft was transplanted into the recipient using end-to-side aortic and portacaval microvascular anastomosis. Procedure duration, animal clinical course and survival were evaluated. Survival shorter than four days was considered technical failure. Recipients were sacrificed with signs of severe graft rejection or survival longer than 120 days. Necropsies were performed in all recipients to access histopathological changes in the graft. RESULTS: Median time for the procedure was 107 minutes. Six recipients (16.7%) presented technical failure. Twenty-seven recipients were sacrificed due to rejection, being nineteen (52.7%) between 7th and 15th postoperative day and eight (22.2%) between 34th and 47th postoperative day. Graft histology confirmed severe acute cellular rejection in those recipients. Uneventful evolution and survival longer than 120 days without rejection were observed in three recipients (8.3%). CONCLUSION: Intestinal transplantation in outbred rats without immunosuppressant regiment accomplishes variable clinical evolution.OBJETIVO: Investigar a evolução clínica do transplante de intestino delgado ortotópico em ratos não-isogênicos. MÉTODOS: Setenta e dois ratos Wistar não-isogênicos, com peso variando entre 250 e 300g, foram utilizados como doadores e receptores em 36 transplantes ortotópicos de intestino delgado sem regime de imunossupressão. Os enxertos foram implantados nos receptores por meio de anastomose microvascular término-lateral aorta-aorta e porto-cava. A duração do procedimento, evolução clínica dos animais e sobrevida foram avaliados. Sobrevida menor que quatro dias foi considerada falha técnica. Os receptores foram sacrificados quando apresentaram sinais de rejeição grave do enxerto ou sobrevida maior que 120 dias. Necropsias foram realizadas em todos os receptores para avaliar alterações histopatológicas no enxerto. RESULTADOS: O tempo médio para o procedimento foi de 107 minutos. Seis receptores (16,7%) apresentaram falha técnica Vinte e sete receptores (75%) foram sacrificados por rejeição sendo dezenove (52,7%) entre o 7º e 15º dia de pós-operatório e oito (22,2%) entre o 34º e 47º. Análise histopatológica confirmou rejeição celular aguda severa nesses recipientes. Evolução sem complicações e sobrevida maior que 120 dias sem sinais de rejeição foi observada em três receptores (8,3%). CONCLUSÃO: O transplante de intestino delgado ortotópico em ratos Wistar não-isogênicos sem regime de imunossupressão apresenta evolução clínica variada.USP FMUSP FM Department of GastroenterologyUSP FM Department of PathologyFederal University of São Paulo Department of Surgery Operative Technique and Experimental Surgery DivisionUNIFESP, Department of Surgery Operative Technique and Experimental Surgery DivisionSciELOSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaUniversidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)Waisberg, Daniel ReisLee, André Dong WongSantos, Rafael Miyashiro Nunes dosMory, Eduardo KenjiCosta, Anderson LinoMontero, Edna Frasson de Souza [UNIFESP]Chaib, EleazarD'albuquerque, Luis Augusto CarneiroGalvao, Flavio Henrique Ferreira2015-06-14T13:43:26Z2015-06-14T13:43:26Z2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion496-502application/pdfhttp://dx.doi.org/10.1590/S0102-86502011000600015Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 26, n. 6, p. 496-502, 2011.10.1590/S0102-86502011000600015S0102-86502011000600015.pdf0102-8650S0102-86502011000600015http://repositorio.unifesp.br/handle/11600/6792WOS:000297571700014engActa Cirurgica Brasileirainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-04T23:43:59Zoai:repositorio.unifesp.br/:11600/6792Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-04T23:43:59Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Small bowel transplantation in outbred rats
Transplante de intestino delgado em ratos não-isogênicos
title Small bowel transplantation in outbred rats
spellingShingle Small bowel transplantation in outbred rats
Waisberg, Daniel Reis
Intestine
Small
Transplantation
Microsurgery
Animals
Outbred Strains
Rats
Intestino Delgado
Transplante
Microcirurgia
Animais não Endogâmicos
Ratos
title_short Small bowel transplantation in outbred rats
title_full Small bowel transplantation in outbred rats
title_fullStr Small bowel transplantation in outbred rats
title_full_unstemmed Small bowel transplantation in outbred rats
title_sort Small bowel transplantation in outbred rats
author Waisberg, Daniel Reis
author_facet Waisberg, Daniel Reis
Lee, André Dong Wong
Santos, Rafael Miyashiro Nunes dos
Mory, Eduardo Kenji
Costa, Anderson Lino
Montero, Edna Frasson de Souza [UNIFESP]
Chaib, Eleazar
D'albuquerque, Luis Augusto Carneiro
Galvao, Flavio Henrique Ferreira
author_role author
author2 Lee, André Dong Wong
Santos, Rafael Miyashiro Nunes dos
Mory, Eduardo Kenji
Costa, Anderson Lino
Montero, Edna Frasson de Souza [UNIFESP]
Chaib, Eleazar
D'albuquerque, Luis Augusto Carneiro
Galvao, Flavio Henrique Ferreira
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Waisberg, Daniel Reis
Lee, André Dong Wong
Santos, Rafael Miyashiro Nunes dos
Mory, Eduardo Kenji
Costa, Anderson Lino
Montero, Edna Frasson de Souza [UNIFESP]
Chaib, Eleazar
D'albuquerque, Luis Augusto Carneiro
Galvao, Flavio Henrique Ferreira
dc.subject.por.fl_str_mv Intestine
Small
Transplantation
Microsurgery
Animals
Outbred Strains
Rats
Intestino Delgado
Transplante
Microcirurgia
Animais não Endogâmicos
Ratos
topic Intestine
Small
Transplantation
Microsurgery
Animals
Outbred Strains
Rats
Intestino Delgado
Transplante
Microcirurgia
Animais não Endogâmicos
Ratos
description PURPOSE: To investigate the clinical evolution of orthotopic small bowel transplantation in outbred rats. METHODS: Seventy-two outbred Wistar rats weighting from 250 to 300g were used as donor and recipient in 36 consecutives ortothopic small intestine transplantation without immunosuppression. The graft was transplanted into the recipient using end-to-side aortic and portacaval microvascular anastomosis. Procedure duration, animal clinical course and survival were evaluated. Survival shorter than four days was considered technical failure. Recipients were sacrificed with signs of severe graft rejection or survival longer than 120 days. Necropsies were performed in all recipients to access histopathological changes in the graft. RESULTS: Median time for the procedure was 107 minutes. Six recipients (16.7%) presented technical failure. Twenty-seven recipients were sacrificed due to rejection, being nineteen (52.7%) between 7th and 15th postoperative day and eight (22.2%) between 34th and 47th postoperative day. Graft histology confirmed severe acute cellular rejection in those recipients. Uneventful evolution and survival longer than 120 days without rejection were observed in three recipients (8.3%). CONCLUSION: Intestinal transplantation in outbred rats without immunosuppressant regiment accomplishes variable clinical evolution.
publishDate 2011
dc.date.none.fl_str_mv 2011-12-01
2015-06-14T13:43:26Z
2015-06-14T13:43:26Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0102-86502011000600015
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 26, n. 6, p. 496-502, 2011.
10.1590/S0102-86502011000600015
S0102-86502011000600015.pdf
0102-8650
S0102-86502011000600015
http://repositorio.unifesp.br/handle/11600/6792
WOS:000297571700014
url http://dx.doi.org/10.1590/S0102-86502011000600015
http://repositorio.unifesp.br/handle/11600/6792
identifier_str_mv Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 26, n. 6, p. 496-502, 2011.
10.1590/S0102-86502011000600015
S0102-86502011000600015.pdf
0102-8650
S0102-86502011000600015
WOS:000297571700014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Cirurgica Brasileira
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 496-502
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268449457700864

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