Comparative recognition by human IgG antibodies of recombinant proteins representing three asexual erythrocytic stage vaccine candidates of Plasmodium vivax
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1590/S0074-02762007005000040 http://repositorio.unifesp.br/handle/11600/3705 |
Resumo: | In previous immuno-epidemiological studies of the naturally acquired antibody responses to merozoite surface protein-1 (MSP-1) of Plasmodium vivax, we had evidence that the responses to distinct erythrocytic stage antigens could be differentially regulated. The present study was designed to compare the antibody response to three asexual erythrocytic stage antigens vaccine candidates of P. vivax. Recombinant proteins representing the 19 kDa C-terminal region of MSP-1(PvMSP19), apical membrane antigen n-1 ectodomain (PvAMA-1), and the region II of duffy binding protein (PvDBP-RII) were compared in their ability to bind to IgG antibodies of serum samples collected from 220 individuals from the state of Pará, in the North of Brazil. During patent infection with P. vivax, the frequency of individuals with IgG antibodies to PvMSP1(19), PvAMA-1, and PvDBP-RII were 95, 72.7, and 44.5% respectively. Although the frequency of responders to PvDBP-RII was lower, this frequency increased in individuals following multiple malarial infections. Individually, the specific antibody levels did not decline significantly nine months after treatment, except to PvMSP1(19). Our results further confirm a complex regulation of the immune response to distinct blood stage antigens. The reason for that is presently unknown but it may contribute to the high risk of re-infection in individuals living in the endemic areas. |
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Comparative recognition by human IgG antibodies of recombinant proteins representing three asexual erythrocytic stage vaccine candidates of Plasmodium vivaxmalariaPlasmodium vivaxmerozoite antigensIgG antibody responseIn previous immuno-epidemiological studies of the naturally acquired antibody responses to merozoite surface protein-1 (MSP-1) of Plasmodium vivax, we had evidence that the responses to distinct erythrocytic stage antigens could be differentially regulated. The present study was designed to compare the antibody response to three asexual erythrocytic stage antigens vaccine candidates of P. vivax. Recombinant proteins representing the 19 kDa C-terminal region of MSP-1(PvMSP19), apical membrane antigen n-1 ectodomain (PvAMA-1), and the region II of duffy binding protein (PvDBP-RII) were compared in their ability to bind to IgG antibodies of serum samples collected from 220 individuals from the state of Pará, in the North of Brazil. During patent infection with P. vivax, the frequency of individuals with IgG antibodies to PvMSP1(19), PvAMA-1, and PvDBP-RII were 95, 72.7, and 44.5% respectively. Although the frequency of responders to PvDBP-RII was lower, this frequency increased in individuals following multiple malarial infections. Individually, the specific antibody levels did not decline significantly nine months after treatment, except to PvMSP1(19). Our results further confirm a complex regulation of the immune response to distinct blood stage antigens. The reason for that is presently unknown but it may contribute to the high risk of re-infection in individuals living in the endemic areas.Universidade de São Paulo Faculdade de Ciências Farmacêuticas Departamento de Análises Clínicas e ToxicológicasUniversidade Federal do Pará Centro de Ciências Biológicas Departamento de PatologiaInternational Centre for Genetic Engineering and Biotechnology Centro de Ciências Biológicas Malaria Research GroupUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Microbiologia, Imunologia e ParasitologiaUNIFESP, EPM, Depto. de Microbiologia, Imunologia e ParasitologiaSciELOInstituto Oswaldo Cruz, Ministério da SaúdeUniversidade de São Paulo (USP)Universidade Federal do Pará Centro de Ciências Biológicas Departamento de PatologiaInternational Centre for Genetic Engineering and Biotechnology Centro de Ciências Biológicas Malaria Research GroupUniversidade Federal de São Paulo (UNIFESP)Barbedo, Mayara BRicci, RicardoJimenez, Maria Carolina SCunha, Maristela GYazdani, Syed SChitnis, Chetan ERodrigues, Mauricio Martins [UNIFESP]Soares, Irene S2015-06-14T13:36:54Z2015-06-14T13:36:54Z2007-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion335-340application/pdfhttp://dx.doi.org/10.1590/S0074-02762007005000040Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 102, n. 3, p. 335-340, 2007.10.1590/S0074-02762007005000040S0074-02762007000300013.pdf0074-0276S0074-02762007000300013http://repositorio.unifesp.br/handle/11600/3705engMemórias do Instituto Oswaldo Cruzinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-29T15:32:39Zoai:repositorio.unifesp.br/:11600/3705Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-29T15:32:39Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Comparative recognition by human IgG antibodies of recombinant proteins representing three asexual erythrocytic stage vaccine candidates of Plasmodium vivax |
title |
Comparative recognition by human IgG antibodies of recombinant proteins representing three asexual erythrocytic stage vaccine candidates of Plasmodium vivax |
spellingShingle |
Comparative recognition by human IgG antibodies of recombinant proteins representing three asexual erythrocytic stage vaccine candidates of Plasmodium vivax Barbedo, Mayara B malaria Plasmodium vivax merozoite antigens IgG antibody response |
title_short |
Comparative recognition by human IgG antibodies of recombinant proteins representing three asexual erythrocytic stage vaccine candidates of Plasmodium vivax |
title_full |
Comparative recognition by human IgG antibodies of recombinant proteins representing three asexual erythrocytic stage vaccine candidates of Plasmodium vivax |
title_fullStr |
Comparative recognition by human IgG antibodies of recombinant proteins representing three asexual erythrocytic stage vaccine candidates of Plasmodium vivax |
title_full_unstemmed |
Comparative recognition by human IgG antibodies of recombinant proteins representing three asexual erythrocytic stage vaccine candidates of Plasmodium vivax |
title_sort |
Comparative recognition by human IgG antibodies of recombinant proteins representing three asexual erythrocytic stage vaccine candidates of Plasmodium vivax |
author |
Barbedo, Mayara B |
author_facet |
Barbedo, Mayara B Ricci, Ricardo Jimenez, Maria Carolina S Cunha, Maristela G Yazdani, Syed S Chitnis, Chetan E Rodrigues, Mauricio Martins [UNIFESP] Soares, Irene S |
author_role |
author |
author2 |
Ricci, Ricardo Jimenez, Maria Carolina S Cunha, Maristela G Yazdani, Syed S Chitnis, Chetan E Rodrigues, Mauricio Martins [UNIFESP] Soares, Irene S |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Federal do Pará Centro de Ciências Biológicas Departamento de Patologia International Centre for Genetic Engineering and Biotechnology Centro de Ciências Biológicas Malaria Research Group Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Barbedo, Mayara B Ricci, Ricardo Jimenez, Maria Carolina S Cunha, Maristela G Yazdani, Syed S Chitnis, Chetan E Rodrigues, Mauricio Martins [UNIFESP] Soares, Irene S |
dc.subject.por.fl_str_mv |
malaria Plasmodium vivax merozoite antigens IgG antibody response |
topic |
malaria Plasmodium vivax merozoite antigens IgG antibody response |
description |
In previous immuno-epidemiological studies of the naturally acquired antibody responses to merozoite surface protein-1 (MSP-1) of Plasmodium vivax, we had evidence that the responses to distinct erythrocytic stage antigens could be differentially regulated. The present study was designed to compare the antibody response to three asexual erythrocytic stage antigens vaccine candidates of P. vivax. Recombinant proteins representing the 19 kDa C-terminal region of MSP-1(PvMSP19), apical membrane antigen n-1 ectodomain (PvAMA-1), and the region II of duffy binding protein (PvDBP-RII) were compared in their ability to bind to IgG antibodies of serum samples collected from 220 individuals from the state of Pará, in the North of Brazil. During patent infection with P. vivax, the frequency of individuals with IgG antibodies to PvMSP1(19), PvAMA-1, and PvDBP-RII were 95, 72.7, and 44.5% respectively. Although the frequency of responders to PvDBP-RII was lower, this frequency increased in individuals following multiple malarial infections. Individually, the specific antibody levels did not decline significantly nine months after treatment, except to PvMSP1(19). Our results further confirm a complex regulation of the immune response to distinct blood stage antigens. The reason for that is presently unknown but it may contribute to the high risk of re-infection in individuals living in the endemic areas. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-06-01 2015-06-14T13:36:54Z 2015-06-14T13:36:54Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0074-02762007005000040 Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 102, n. 3, p. 335-340, 2007. 10.1590/S0074-02762007005000040 S0074-02762007000300013.pdf 0074-0276 S0074-02762007000300013 http://repositorio.unifesp.br/handle/11600/3705 |
url |
http://dx.doi.org/10.1590/S0074-02762007005000040 http://repositorio.unifesp.br/handle/11600/3705 |
identifier_str_mv |
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 102, n. 3, p. 335-340, 2007. 10.1590/S0074-02762007005000040 S0074-02762007000300013.pdf 0074-0276 S0074-02762007000300013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
335-340 application/pdf |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268414412193792 |