A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry

Detalhes bibliográficos
Autor(a) principal: Farias, Elizangela
Data de Publicação: 2019
Outros Autores: Bezerra, Fhabiane, Silva, Djane Clarys Baia da, Chaves, Yuri Oliveira, Cardoza, Tatiana Bacry, Almeida, Maria Edilene Martins de, Oliveira, Lucas Barbosa, Lalwani, Pritesh, Orlandi, Patrícia Puccinelli, Lacerda, Lucas Barbosa, Lopes, Stefanie Costa Pinto, Nogueira, Paulo Afonso
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/43840
Resumo: Universidade Federal do Amazonas, Programa de Pós-Graduação Stricto Sensu em Imunologia Básica e Aplicada, Manaus. AM, Brasil / Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Programa de Pós-Graduação Stricto Sensu em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil.
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spelling Farias, ElizangelaBezerra, FhabianeSilva, Djane Clarys Baia daChaves, Yuri OliveiraCardoza, Tatiana BacryAlmeida, Maria Edilene Martins deOliveira, Lucas BarbosaLalwani, PriteshOrlandi, Patrícia PuccinelliLacerda, Lucas BarbosaLopes, Stefanie Costa PintoNogueira, Paulo Afonso2020-10-06T20:01:30Z2020-10-06T20:01:30Z2019FARIAS, Elizangela et al. A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, e190158, 5p, 2019.0074-0276https://www.arca.fiocruz.br/handle/icict/4384010.1590/0074-027601901581678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Plasmodium vivaxAnticorpos opssonantesMerozoitasMSP1Plasmodium vivaxMerozoitesMSP1Ipsonising antibodiesA simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Amazonas, Programa de Pós-Graduação Stricto Sensu em Imunologia Básica e Aplicada, Manaus. AM, Brasil / Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Programa de Pós-Graduação Stricto Sensu em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil.Universidade Federal do Amazonas, Programa de Pós-Graduação Stricto Sensu em Imunologia Básica e Aplicada, Manaus. AM, Brasil .Fundação de Medicina Tropical Dr Heitor Vieira Dourado. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Programa de Pós-Graduação Stricto Sensu em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil / Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Programa de Pós-Graduação Stricto Sensu em Biologia Parasitária, Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Programa de Pós-Graduação Stricto Sensu em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Programa de Pós-Graduação Stricto Sensu em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa de Pós-Graduação Stricto Sensu em Biologia Celular e Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Programa de Pós-Graduação Stricto Sensu em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Programa de Pós-Graduação Stricto Sensu em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Programa de Pós-Graduação Stricto Sensu em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil.Universidade Federal do Amazonas, Programa de Pós-Graduação Stricto Sensu em Imunologia Básica e Aplicada, Manaus, AM, Brasil /Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Programa de Pós-Graduação Stricto Sensu em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Programa de Pós-Graduação Stricto Sensu em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil / Fundação de Medicina Tropical Dr Heitor Vieira Dourado. Manaus, AM, Brasil..Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Programa de Pós-Graduação Stricto Sensu em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Programa de Pós-Graduação Stricto Sensu em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil.As phagocytosis is the first line of defense against malaria, we developed a phagocytosis assay with Plasmodium vivax (P. vivax) merozoites that can be applied to evaluate vaccine candidates. Briefly, after leukocyte removal with loosely packed cellulose powder in a syringe, P. vivax trophozoites matured to the merozoite-rich schizont stages in the presence of the E64 protease inhibitor. The Percoll gradient-enriched schizonts were chemically disrupted to release merozoites that were submitted to merozoite opsonin-dependent phagocytosis in two phagocytic lines with human and mouse antibodies against the N- and C-terminus of P. vivax Merozoite Surface Protein-1 (Nterm-PvMSP1 and MSP119). The resulting assay is simple and efficient for use as a routine phagocytic assay for the evaluation of merozoite stage vaccine candidates.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/43840/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALYuriChaves_MariaEdileneAlmeida_etal_IOC_2019.pdfYuriChaves_MariaEdileneAlmeida_etal_IOC_2019.pdfapplication/pdf2323278https://www.arca.fiocruz.br/bitstream/icict/43840/2/YuriChaves_MariaEdileneAlmeida_etal_IOC_2019.pdf46688931fb14d0bd7bb992f83b26ccd5MD52TEXTYuriChaves_MariaEdileneAlmeida_etal_IOC_2019.pdf.txtYuriChaves_MariaEdileneAlmeida_etal_IOC_2019.pdf.txtExtracted texttext/plain23622https://www.arca.fiocruz.br/bitstream/icict/43840/3/YuriChaves_MariaEdileneAlmeida_etal_IOC_2019.pdf.txtc0fdec93daaa0613eec3325d36f7d3b8MD53icict/438402020-10-07 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dc.title.pt_BR.fl_str_mv A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry
title A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry
spellingShingle A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry
Farias, Elizangela
Plasmodium vivax
Anticorpos opssonantes
Merozoitas
MSP1
Plasmodium vivax
Merozoites
MSP1
Ipsonising antibodies
title_short A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry
title_full A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry
title_fullStr A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry
title_full_unstemmed A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry
title_sort A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry
author Farias, Elizangela
author_facet Farias, Elizangela
Bezerra, Fhabiane
Silva, Djane Clarys Baia da
Chaves, Yuri Oliveira
Cardoza, Tatiana Bacry
Almeida, Maria Edilene Martins de
Oliveira, Lucas Barbosa
Lalwani, Pritesh
Orlandi, Patrícia Puccinelli
Lacerda, Lucas Barbosa
Lopes, Stefanie Costa Pinto
Nogueira, Paulo Afonso
author_role author
author2 Bezerra, Fhabiane
Silva, Djane Clarys Baia da
Chaves, Yuri Oliveira
Cardoza, Tatiana Bacry
Almeida, Maria Edilene Martins de
Oliveira, Lucas Barbosa
Lalwani, Pritesh
Orlandi, Patrícia Puccinelli
Lacerda, Lucas Barbosa
Lopes, Stefanie Costa Pinto
Nogueira, Paulo Afonso
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Farias, Elizangela
Bezerra, Fhabiane
Silva, Djane Clarys Baia da
Chaves, Yuri Oliveira
Cardoza, Tatiana Bacry
Almeida, Maria Edilene Martins de
Oliveira, Lucas Barbosa
Lalwani, Pritesh
Orlandi, Patrícia Puccinelli
Lacerda, Lucas Barbosa
Lopes, Stefanie Costa Pinto
Nogueira, Paulo Afonso
dc.subject.other.pt_BR.fl_str_mv Plasmodium vivax
Anticorpos opssonantes
Merozoitas
MSP1
topic Plasmodium vivax
Anticorpos opssonantes
Merozoitas
MSP1
Plasmodium vivax
Merozoites
MSP1
Ipsonising antibodies
dc.subject.en.pt_BR.fl_str_mv Plasmodium vivax
Merozoites
MSP1
Ipsonising antibodies
description Universidade Federal do Amazonas, Programa de Pós-Graduação Stricto Sensu em Imunologia Básica e Aplicada, Manaus. AM, Brasil / Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Programa de Pós-Graduação Stricto Sensu em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil.
publishDate 2019
dc.date.issued.fl_str_mv 2019
dc.date.accessioned.fl_str_mv 2020-10-06T20:01:30Z
dc.date.available.fl_str_mv 2020-10-06T20:01:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FARIAS, Elizangela et al. A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, e190158, 5p, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/43840
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760190158
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv FARIAS, Elizangela et al. A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, e190158, 5p, 2019.
0074-0276
10.1590/0074-02760190158
1678-8060
url https://www.arca.fiocruz.br/handle/icict/43840
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/43840/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/43840/2/YuriChaves_MariaEdileneAlmeida_etal_IOC_2019.pdf
https://www.arca.fiocruz.br/bitstream/icict/43840/3/YuriChaves_MariaEdileneAlmeida_etal_IOC_2019.pdf.txt
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