A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
DOI: | 10.1590/0074-02760190158 |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/43840 |
Resumo: | Universidade Federal do Amazonas, Programa de Pós-Graduação Stricto Sensu em Imunologia Básica e Aplicada, Manaus. AM, Brasil / Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Programa de Pós-Graduação Stricto Sensu em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil. |
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Farias, ElizangelaBezerra, FhabianeSilva, Djane Clarys Baia daChaves, Yuri OliveiraCardoza, Tatiana BacryAlmeida, Maria Edilene Martins deOliveira, Lucas BarbosaLalwani, PriteshOrlandi, Patrícia PuccinelliLacerda, Lucas BarbosaLopes, Stefanie Costa PintoNogueira, Paulo Afonso2020-10-06T20:01:30Z2020-10-06T20:01:30Z2019FARIAS, Elizangela et al. A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, e190158, 5p, 2019.0074-0276https://www.arca.fiocruz.br/handle/icict/4384010.1590/0074-027601901581678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Plasmodium vivaxAnticorpos opssonantesMerozoitasMSP1Plasmodium vivaxMerozoitesMSP1Ipsonising antibodiesA simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Amazonas, Programa de Pós-Graduação Stricto Sensu em Imunologia Básica e Aplicada, Manaus. AM, Brasil / Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Programa de Pós-Graduação Stricto Sensu em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil.Universidade Federal do Amazonas, Programa de Pós-Graduação Stricto Sensu em Imunologia Básica e Aplicada, Manaus. AM, Brasil .Fundação de Medicina Tropical Dr Heitor Vieira Dourado. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Programa de Pós-Graduação Stricto Sensu em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil / Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Programa de Pós-Graduação Stricto Sensu em Biologia Parasitária, Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Programa de Pós-Graduação Stricto Sensu em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Programa de Pós-Graduação Stricto Sensu em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa de Pós-Graduação Stricto Sensu em Biologia Celular e Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Programa de Pós-Graduação Stricto Sensu em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Programa de Pós-Graduação Stricto Sensu em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Programa de Pós-Graduação Stricto Sensu em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil.Universidade Federal do Amazonas, Programa de Pós-Graduação Stricto Sensu em Imunologia Básica e Aplicada, Manaus, AM, Brasil /Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Programa de Pós-Graduação Stricto Sensu em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Programa de Pós-Graduação Stricto Sensu em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil / Fundação de Medicina Tropical Dr Heitor Vieira Dourado. Manaus, AM, Brasil..Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Programa de Pós-Graduação Stricto Sensu em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Programa de Pós-Graduação Stricto Sensu em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil.As phagocytosis is the first line of defense against malaria, we developed a phagocytosis assay with Plasmodium vivax (P. vivax) merozoites that can be applied to evaluate vaccine candidates. Briefly, after leukocyte removal with loosely packed cellulose powder in a syringe, P. vivax trophozoites matured to the merozoite-rich schizont stages in the presence of the E64 protease inhibitor. The Percoll gradient-enriched schizonts were chemically disrupted to release merozoites that were submitted to merozoite opsonin-dependent phagocytosis in two phagocytic lines with human and mouse antibodies against the N- and C-terminus of P. vivax Merozoite Surface Protein-1 (Nterm-PvMSP1 and MSP119). The resulting assay is simple and efficient for use as a routine phagocytic assay for the evaluation of merozoite stage vaccine candidates.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/43840/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALYuriChaves_MariaEdileneAlmeida_etal_IOC_2019.pdfYuriChaves_MariaEdileneAlmeida_etal_IOC_2019.pdfapplication/pdf2323278https://www.arca.fiocruz.br/bitstream/icict/43840/2/YuriChaves_MariaEdileneAlmeida_etal_IOC_2019.pdf46688931fb14d0bd7bb992f83b26ccd5MD52TEXTYuriChaves_MariaEdileneAlmeida_etal_IOC_2019.pdf.txtYuriChaves_MariaEdileneAlmeida_etal_IOC_2019.pdf.txtExtracted texttext/plain23622https://www.arca.fiocruz.br/bitstream/icict/43840/3/YuriChaves_MariaEdileneAlmeida_etal_IOC_2019.pdf.txtc0fdec93daaa0613eec3325d36f7d3b8MD53icict/438402020-10-07 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dc.title.pt_BR.fl_str_mv |
A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry |
title |
A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry |
spellingShingle |
A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry Farias, Elizangela Plasmodium vivax Anticorpos opssonantes Merozoitas MSP1 Plasmodium vivax Merozoites MSP1 Ipsonising antibodies |
title_short |
A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry |
title_full |
A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry |
title_fullStr |
A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry |
title_full_unstemmed |
A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry |
title_sort |
A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry |
author |
Farias, Elizangela |
author_facet |
Farias, Elizangela Bezerra, Fhabiane Silva, Djane Clarys Baia da Chaves, Yuri Oliveira Cardoza, Tatiana Bacry Almeida, Maria Edilene Martins de Oliveira, Lucas Barbosa Lalwani, Pritesh Orlandi, Patrícia Puccinelli Lacerda, Lucas Barbosa Lopes, Stefanie Costa Pinto Nogueira, Paulo Afonso |
author_role |
author |
author2 |
Bezerra, Fhabiane Silva, Djane Clarys Baia da Chaves, Yuri Oliveira Cardoza, Tatiana Bacry Almeida, Maria Edilene Martins de Oliveira, Lucas Barbosa Lalwani, Pritesh Orlandi, Patrícia Puccinelli Lacerda, Lucas Barbosa Lopes, Stefanie Costa Pinto Nogueira, Paulo Afonso |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Farias, Elizangela Bezerra, Fhabiane Silva, Djane Clarys Baia da Chaves, Yuri Oliveira Cardoza, Tatiana Bacry Almeida, Maria Edilene Martins de Oliveira, Lucas Barbosa Lalwani, Pritesh Orlandi, Patrícia Puccinelli Lacerda, Lucas Barbosa Lopes, Stefanie Costa Pinto Nogueira, Paulo Afonso |
dc.subject.other.pt_BR.fl_str_mv |
Plasmodium vivax Anticorpos opssonantes Merozoitas MSP1 |
topic |
Plasmodium vivax Anticorpos opssonantes Merozoitas MSP1 Plasmodium vivax Merozoites MSP1 Ipsonising antibodies |
dc.subject.en.pt_BR.fl_str_mv |
Plasmodium vivax Merozoites MSP1 Ipsonising antibodies |
description |
Universidade Federal do Amazonas, Programa de Pós-Graduação Stricto Sensu em Imunologia Básica e Aplicada, Manaus. AM, Brasil / Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Programa de Pós-Graduação Stricto Sensu em Biologia da Interação Patógeno Hospedeiro. Manaus, AM, Brasil. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019 |
dc.date.accessioned.fl_str_mv |
2020-10-06T20:01:30Z |
dc.date.available.fl_str_mv |
2020-10-06T20:01:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FARIAS, Elizangela et al. A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, e190158, 5p, 2019. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/43840 |
dc.identifier.issn.pt_BR.fl_str_mv |
0074-0276 |
dc.identifier.doi.none.fl_str_mv |
10.1590/0074-02760190158 |
dc.identifier.eissn.none.fl_str_mv |
1678-8060 |
identifier_str_mv |
FARIAS, Elizangela et al. A simple, ex vivo phagocytosis assay of Plasmodium vivax merozoites by flow cytometry. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, e190158, 5p, 2019. 0074-0276 10.1590/0074-02760190158 1678-8060 |
url |
https://www.arca.fiocruz.br/handle/icict/43840 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. |
publisher.none.fl_str_mv |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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Fundação Oswaldo Cruz (FIOCRUZ) |
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FIOCRUZ |
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Repositório Institucional da FIOCRUZ (ARCA) |
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repositorio.arca@fiocruz.br |
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