Two physically, functionally, and developmentally distinct peritoneal macrophage subsets
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/32262 http://dx.doi.org/10.1073/pnas.0915000107 |
Resumo: | The peritoneal cavity (PerC) is a unique compartment within which a variety of immune cells reside, and from which macrophages (Mempty set) are commonly drawn for functional studies. Here we define two Mempty set subsets that coexist in PerC in adult mice. One, provisionally called the large peritoneal Mempty set (LPM), contains approximately 90% of the PerC Mempty set in unstimulated animals but disappears rapidly from PerC following lipopolysaccharide (LPS) or thioglycolate stimulation. These cells express high levels of the canonical Mempty set surface markers, CD11b and F4/80. the second subset, referred to as small peritoneal Mempty set (SPM), expresses substantially lower levels of CD11b and F4/80 but expresses high levels of MHC-II, which is not expressed on LPM. SPM, which predominates in PerC after LPS or thioglycolate stimulation, does not derive from LPM. Instead, it derives from blood monocytes that rapidly enter the PerC after stimulation and differentiate to mature SPM within 2 to 4 d. Both subsets show clear phagocytic activity and both produce nitric oxide (NO) in response to LPS stimulation in vivo. However, their responses to LPS show key differences: in vitro, LPS stimulates LPM, but not SPM, to produce NO; in vivo, LPS stimulates both subsets to produce NO, albeit with different response patterns. These findings extend current models of Mempty set heterogeneity and shed new light on PerC Mempty set diversity, development, and function. Thus, they introduce a new context for interpreting (and reinterpreting) data from ex vivo studies with PerC Mempty set. |
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Ghosn, Eliver Eid BouCassado, Alexandra A.Govoni, Gregory R.Fukuhara, TakeshiYang, YangMonack, Denise M.Bortoluci, Karina Ramalho [UNIFESP]Almeida, Sandro R.Herzenberg, LeonardHerzenberg, Leonore A.Stanford UnivUniversidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)2016-01-24T13:59:19Z2016-01-24T13:59:19Z2010-02-09Proceedings of the National Academy of Sciences of the United States of America. Washington: Natl Acad Sciences, v. 107, n. 6, p. 2568-2573, 2010.0027-8424http://repositorio.unifesp.br/handle/11600/32262http://dx.doi.org/10.1073/pnas.091500010710.1073/pnas.0915000107WOS:000274408100039The peritoneal cavity (PerC) is a unique compartment within which a variety of immune cells reside, and from which macrophages (Mempty set) are commonly drawn for functional studies. Here we define two Mempty set subsets that coexist in PerC in adult mice. One, provisionally called the large peritoneal Mempty set (LPM), contains approximately 90% of the PerC Mempty set in unstimulated animals but disappears rapidly from PerC following lipopolysaccharide (LPS) or thioglycolate stimulation. These cells express high levels of the canonical Mempty set surface markers, CD11b and F4/80. the second subset, referred to as small peritoneal Mempty set (SPM), expresses substantially lower levels of CD11b and F4/80 but expresses high levels of MHC-II, which is not expressed on LPM. SPM, which predominates in PerC after LPS or thioglycolate stimulation, does not derive from LPM. Instead, it derives from blood monocytes that rapidly enter the PerC after stimulation and differentiate to mature SPM within 2 to 4 d. Both subsets show clear phagocytic activity and both produce nitric oxide (NO) in response to LPS stimulation in vivo. However, their responses to LPS show key differences: in vitro, LPS stimulates LPM, but not SPM, to produce NO; in vivo, LPS stimulates both subsets to produce NO, albeit with different response patterns. These findings extend current models of Mempty set heterogeneity and shed new light on PerC Mempty set diversity, development, and function. Thus, they introduce a new context for interpreting (and reinterpreting) data from ex vivo studies with PerC Mempty set.National Institutes of HealthStanford Univ, Sch Med, Dept Genet, Stanford, CA 94305 USAUniv São Paulo, Fac Pharmaceut Sci, Dept Clin Anal, BR-05588 São Paulo, BrazilUniv São Paulo, Inst Biol Sci ICB IV, Dept Immunol, BR-05588 São Paulo, BrazilStanford Univ, Sch Med, Dept Microbiol & Immunol, Stanford, CA 94305 USAUniversidade Federal de São Paulo, Dept Biol Sci, BR-09972 Diadema, SP, BrazilUniversidade Federal de São Paulo, Dept Biol Sci, BR-09972 Diadema, SP, BrazilNational Institutes of Health: AI076434Web of Science2568-2573engNatl Acad SciencesProceedings of the National Academy of Sciences of the United States of AmericaCD11bF4/80lipopolysaccharideperitoneal cavitythioglycolateTwo physically, functionally, and developmentally distinct peritoneal macrophage subsetsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/322622023-01-27 22:45:17.202metadata only accessoai:repositorio.unifesp.br:11600/32262Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-01-28T01:45:17Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Two physically, functionally, and developmentally distinct peritoneal macrophage subsets |
title |
Two physically, functionally, and developmentally distinct peritoneal macrophage subsets |
spellingShingle |
Two physically, functionally, and developmentally distinct peritoneal macrophage subsets Ghosn, Eliver Eid Bou CD11b F4/80 lipopolysaccharide peritoneal cavity thioglycolate |
title_short |
Two physically, functionally, and developmentally distinct peritoneal macrophage subsets |
title_full |
Two physically, functionally, and developmentally distinct peritoneal macrophage subsets |
title_fullStr |
Two physically, functionally, and developmentally distinct peritoneal macrophage subsets |
title_full_unstemmed |
Two physically, functionally, and developmentally distinct peritoneal macrophage subsets |
title_sort |
Two physically, functionally, and developmentally distinct peritoneal macrophage subsets |
author |
Ghosn, Eliver Eid Bou |
author_facet |
Ghosn, Eliver Eid Bou Cassado, Alexandra A. Govoni, Gregory R. Fukuhara, Takeshi Yang, Yang Monack, Denise M. Bortoluci, Karina Ramalho [UNIFESP] Almeida, Sandro R. Herzenberg, Leonard Herzenberg, Leonore A. |
author_role |
author |
author2 |
Cassado, Alexandra A. Govoni, Gregory R. Fukuhara, Takeshi Yang, Yang Monack, Denise M. Bortoluci, Karina Ramalho [UNIFESP] Almeida, Sandro R. Herzenberg, Leonard Herzenberg, Leonore A. |
author2_role |
author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Stanford Univ Universidade de São Paulo (USP) Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Ghosn, Eliver Eid Bou Cassado, Alexandra A. Govoni, Gregory R. Fukuhara, Takeshi Yang, Yang Monack, Denise M. Bortoluci, Karina Ramalho [UNIFESP] Almeida, Sandro R. Herzenberg, Leonard Herzenberg, Leonore A. |
dc.subject.eng.fl_str_mv |
CD11b F4/80 lipopolysaccharide peritoneal cavity thioglycolate |
topic |
CD11b F4/80 lipopolysaccharide peritoneal cavity thioglycolate |
description |
The peritoneal cavity (PerC) is a unique compartment within which a variety of immune cells reside, and from which macrophages (Mempty set) are commonly drawn for functional studies. Here we define two Mempty set subsets that coexist in PerC in adult mice. One, provisionally called the large peritoneal Mempty set (LPM), contains approximately 90% of the PerC Mempty set in unstimulated animals but disappears rapidly from PerC following lipopolysaccharide (LPS) or thioglycolate stimulation. These cells express high levels of the canonical Mempty set surface markers, CD11b and F4/80. the second subset, referred to as small peritoneal Mempty set (SPM), expresses substantially lower levels of CD11b and F4/80 but expresses high levels of MHC-II, which is not expressed on LPM. SPM, which predominates in PerC after LPS or thioglycolate stimulation, does not derive from LPM. Instead, it derives from blood monocytes that rapidly enter the PerC after stimulation and differentiate to mature SPM within 2 to 4 d. Both subsets show clear phagocytic activity and both produce nitric oxide (NO) in response to LPS stimulation in vivo. However, their responses to LPS show key differences: in vitro, LPS stimulates LPM, but not SPM, to produce NO; in vivo, LPS stimulates both subsets to produce NO, albeit with different response patterns. These findings extend current models of Mempty set heterogeneity and shed new light on PerC Mempty set diversity, development, and function. Thus, they introduce a new context for interpreting (and reinterpreting) data from ex vivo studies with PerC Mempty set. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-02-09 |
dc.date.accessioned.fl_str_mv |
2016-01-24T13:59:19Z |
dc.date.available.fl_str_mv |
2016-01-24T13:59:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Proceedings of the National Academy of Sciences of the United States of America. Washington: Natl Acad Sciences, v. 107, n. 6, p. 2568-2573, 2010. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/32262 http://dx.doi.org/10.1073/pnas.0915000107 |
dc.identifier.issn.none.fl_str_mv |
0027-8424 |
dc.identifier.doi.none.fl_str_mv |
10.1073/pnas.0915000107 |
dc.identifier.wos.none.fl_str_mv |
WOS:000274408100039 |
identifier_str_mv |
Proceedings of the National Academy of Sciences of the United States of America. Washington: Natl Acad Sciences, v. 107, n. 6, p. 2568-2573, 2010. 0027-8424 10.1073/pnas.0915000107 WOS:000274408100039 |
url |
http://repositorio.unifesp.br/handle/11600/32262 http://dx.doi.org/10.1073/pnas.0915000107 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Proceedings of the National Academy of Sciences of the United States of America |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2568-2573 |
dc.publisher.none.fl_str_mv |
Natl Acad Sciences |
publisher.none.fl_str_mv |
Natl Acad Sciences |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764276373389312 |