Fever temperature enhances mechanisms of survival of Streptococcus agalactiae within human endothelial cells

Detalhes bibliográficos
Autor(a) principal: Freitas Lione, Viviane Oliveira
Data de Publicação: 2010
Outros Autores: Bittencourt Dos Santos, Michelle Hanthequeste, Ulisses Carvalho, Tecia Maria, Hirata, Raphael, Mattos-Guaraldi, Ana Luiza, Mortara, Renato Arruda [UNIFESP], Nagao, Prescilla Emy
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.3892/ijmm_00000493
http://repositorio.unifesp.br/handle/11600/32937
Resumo: Group B streptococci (GBS) are the most common cause of pneumonia and sepsis during the neonatal period. However, the pathogenesis of invasive infection is poorly understood. We investigated the ability of GBS grown at 37 C and 40 C to adhere and invade human umbilical vein endothelial cells (HUVECs) at different periods of incubation (0, 0.5, 1, 2, 18 and 24 h). All strains tested, except strain 88641-vagina survived for 24 h in the intracellular environment at 40 degrees C. for serotype III grown at 40 degrees C, both strains (80340-vagina and 90356-liquor) showed increased adherence and intracellular survival when compared to bacteria grown at 37 degrees C (P<0.01). GBS serotype V strains (88641-vagina and 90186-blood) showed ability to survive inside HUVECs until 2 and 24 h post-infection at 40 degrees C and 37 degrees C, respectively (P<0.01). Influence of growth temperature in bacterial interaction with endothelial cells was partially dependent of serotypes and the clinical origin of strains. Serotypes III and V strains grown at both temperatures remained viable within acidic endothelial vacuoles which acquired Rab7 and LAMP-1 endosomal markers. the data emphasize the influence of temperature on cellular events of phagocytosis and pathogenesis of GBS diseases.
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spelling Fever temperature enhances mechanisms of survival of Streptococcus agalactiae within human endothelial cellsStreptococcus ugalactiaegrowth temperatureendothelial cellsfusion lysosomes-phagocytic vacuolesGroup B streptococci (GBS) are the most common cause of pneumonia and sepsis during the neonatal period. However, the pathogenesis of invasive infection is poorly understood. We investigated the ability of GBS grown at 37 C and 40 C to adhere and invade human umbilical vein endothelial cells (HUVECs) at different periods of incubation (0, 0.5, 1, 2, 18 and 24 h). All strains tested, except strain 88641-vagina survived for 24 h in the intracellular environment at 40 degrees C. for serotype III grown at 40 degrees C, both strains (80340-vagina and 90356-liquor) showed increased adherence and intracellular survival when compared to bacteria grown at 37 degrees C (P<0.01). GBS serotype V strains (88641-vagina and 90186-blood) showed ability to survive inside HUVECs until 2 and 24 h post-infection at 40 degrees C and 37 degrees C, respectively (P<0.01). Influence of growth temperature in bacterial interaction with endothelial cells was partially dependent of serotypes and the clinical origin of strains. Serotypes III and V strains grown at both temperatures remained viable within acidic endothelial vacuoles which acquired Rab7 and LAMP-1 endosomal markers. the data emphasize the influence of temperature on cellular events of phagocytosis and pathogenesis of GBS diseases.UERJ Inst Biol Roberto Alcantara Gomes, Dept Biol Celular, BR-20550013 Rio de Janeiro, BrazilUniv Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Ultraestrutura Celular Hertha Meyer, BR-21941 Rio de Janeiro, BrazilUniv Estado Rio de Janeiro, Fac Ciencias Med, Disciplina Microbiol & Imunol, BR-20551030 Rio de Janeiro, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilWeb of ScienceSpandidos Publ LtdUniversidade do Estado do Rio de Janeiro (UERJ)Universidade Federal do Rio de Janeiro (UFRJ)Universidade Federal de São Paulo (UNIFESP)Freitas Lione, Viviane OliveiraBittencourt Dos Santos, Michelle HanthequesteUlisses Carvalho, Tecia MariaHirata, RaphaelMattos-Guaraldi, Ana LuizaMortara, Renato Arruda [UNIFESP]Nagao, Prescilla Emy2016-01-24T14:05:30Z2016-01-24T14:05:30Z2010-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion511-516http://dx.doi.org/10.3892/ijmm_00000493International Journal of Molecular Medicine. Athens: Spandidos Publ Ltd, v. 26, n. 4, p. 511-516, 2010.10.3892/ijmm_000004931107-3756http://repositorio.unifesp.br/handle/11600/32937WOS:000281917800010engInternational Journal of Molecular Medicineinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2016-01-24T12:05:30Zoai:repositorio.unifesp.br/:11600/32937Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652016-01-24T12:05:30Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Fever temperature enhances mechanisms of survival of Streptococcus agalactiae within human endothelial cells
title Fever temperature enhances mechanisms of survival of Streptococcus agalactiae within human endothelial cells
spellingShingle Fever temperature enhances mechanisms of survival of Streptococcus agalactiae within human endothelial cells
Freitas Lione, Viviane Oliveira
Streptococcus ugalactiae
growth temperature
endothelial cells
fusion lysosomes-phagocytic vacuoles
title_short Fever temperature enhances mechanisms of survival of Streptococcus agalactiae within human endothelial cells
title_full Fever temperature enhances mechanisms of survival of Streptococcus agalactiae within human endothelial cells
title_fullStr Fever temperature enhances mechanisms of survival of Streptococcus agalactiae within human endothelial cells
title_full_unstemmed Fever temperature enhances mechanisms of survival of Streptococcus agalactiae within human endothelial cells
title_sort Fever temperature enhances mechanisms of survival of Streptococcus agalactiae within human endothelial cells
author Freitas Lione, Viviane Oliveira
author_facet Freitas Lione, Viviane Oliveira
Bittencourt Dos Santos, Michelle Hanthequeste
Ulisses Carvalho, Tecia Maria
Hirata, Raphael
Mattos-Guaraldi, Ana Luiza
Mortara, Renato Arruda [UNIFESP]
Nagao, Prescilla Emy
author_role author
author2 Bittencourt Dos Santos, Michelle Hanthequeste
Ulisses Carvalho, Tecia Maria
Hirata, Raphael
Mattos-Guaraldi, Ana Luiza
Mortara, Renato Arruda [UNIFESP]
Nagao, Prescilla Emy
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Estado do Rio de Janeiro (UERJ)
Universidade Federal do Rio de Janeiro (UFRJ)
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Freitas Lione, Viviane Oliveira
Bittencourt Dos Santos, Michelle Hanthequeste
Ulisses Carvalho, Tecia Maria
Hirata, Raphael
Mattos-Guaraldi, Ana Luiza
Mortara, Renato Arruda [UNIFESP]
Nagao, Prescilla Emy
dc.subject.por.fl_str_mv Streptococcus ugalactiae
growth temperature
endothelial cells
fusion lysosomes-phagocytic vacuoles
topic Streptococcus ugalactiae
growth temperature
endothelial cells
fusion lysosomes-phagocytic vacuoles
description Group B streptococci (GBS) are the most common cause of pneumonia and sepsis during the neonatal period. However, the pathogenesis of invasive infection is poorly understood. We investigated the ability of GBS grown at 37 C and 40 C to adhere and invade human umbilical vein endothelial cells (HUVECs) at different periods of incubation (0, 0.5, 1, 2, 18 and 24 h). All strains tested, except strain 88641-vagina survived for 24 h in the intracellular environment at 40 degrees C. for serotype III grown at 40 degrees C, both strains (80340-vagina and 90356-liquor) showed increased adherence and intracellular survival when compared to bacteria grown at 37 degrees C (P<0.01). GBS serotype V strains (88641-vagina and 90186-blood) showed ability to survive inside HUVECs until 2 and 24 h post-infection at 40 degrees C and 37 degrees C, respectively (P<0.01). Influence of growth temperature in bacterial interaction with endothelial cells was partially dependent of serotypes and the clinical origin of strains. Serotypes III and V strains grown at both temperatures remained viable within acidic endothelial vacuoles which acquired Rab7 and LAMP-1 endosomal markers. the data emphasize the influence of temperature on cellular events of phagocytosis and pathogenesis of GBS diseases.
publishDate 2010
dc.date.none.fl_str_mv 2010-10-01
2016-01-24T14:05:30Z
2016-01-24T14:05:30Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3892/ijmm_00000493
International Journal of Molecular Medicine. Athens: Spandidos Publ Ltd, v. 26, n. 4, p. 511-516, 2010.
10.3892/ijmm_00000493
1107-3756
http://repositorio.unifesp.br/handle/11600/32937
WOS:000281917800010
url http://dx.doi.org/10.3892/ijmm_00000493
http://repositorio.unifesp.br/handle/11600/32937
identifier_str_mv International Journal of Molecular Medicine. Athens: Spandidos Publ Ltd, v. 26, n. 4, p. 511-516, 2010.
10.3892/ijmm_00000493
1107-3756
WOS:000281917800010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International Journal of Molecular Medicine
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 511-516
dc.publisher.none.fl_str_mv Spandidos Publ Ltd
publisher.none.fl_str_mv Spandidos Publ Ltd
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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