Fever temperature enhances mechanisms of survival of Streptococcus agalactiae within human endothelial cells
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.3892/ijmm_00000493 http://repositorio.unifesp.br/handle/11600/32937 |
Resumo: | Group B streptococci (GBS) are the most common cause of pneumonia and sepsis during the neonatal period. However, the pathogenesis of invasive infection is poorly understood. We investigated the ability of GBS grown at 37 C and 40 C to adhere and invade human umbilical vein endothelial cells (HUVECs) at different periods of incubation (0, 0.5, 1, 2, 18 and 24 h). All strains tested, except strain 88641-vagina survived for 24 h in the intracellular environment at 40 degrees C. for serotype III grown at 40 degrees C, both strains (80340-vagina and 90356-liquor) showed increased adherence and intracellular survival when compared to bacteria grown at 37 degrees C (P<0.01). GBS serotype V strains (88641-vagina and 90186-blood) showed ability to survive inside HUVECs until 2 and 24 h post-infection at 40 degrees C and 37 degrees C, respectively (P<0.01). Influence of growth temperature in bacterial interaction with endothelial cells was partially dependent of serotypes and the clinical origin of strains. Serotypes III and V strains grown at both temperatures remained viable within acidic endothelial vacuoles which acquired Rab7 and LAMP-1 endosomal markers. the data emphasize the influence of temperature on cellular events of phagocytosis and pathogenesis of GBS diseases. |
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Fever temperature enhances mechanisms of survival of Streptococcus agalactiae within human endothelial cellsStreptococcus ugalactiaegrowth temperatureendothelial cellsfusion lysosomes-phagocytic vacuolesGroup B streptococci (GBS) are the most common cause of pneumonia and sepsis during the neonatal period. However, the pathogenesis of invasive infection is poorly understood. We investigated the ability of GBS grown at 37 C and 40 C to adhere and invade human umbilical vein endothelial cells (HUVECs) at different periods of incubation (0, 0.5, 1, 2, 18 and 24 h). All strains tested, except strain 88641-vagina survived for 24 h in the intracellular environment at 40 degrees C. for serotype III grown at 40 degrees C, both strains (80340-vagina and 90356-liquor) showed increased adherence and intracellular survival when compared to bacteria grown at 37 degrees C (P<0.01). GBS serotype V strains (88641-vagina and 90186-blood) showed ability to survive inside HUVECs until 2 and 24 h post-infection at 40 degrees C and 37 degrees C, respectively (P<0.01). Influence of growth temperature in bacterial interaction with endothelial cells was partially dependent of serotypes and the clinical origin of strains. Serotypes III and V strains grown at both temperatures remained viable within acidic endothelial vacuoles which acquired Rab7 and LAMP-1 endosomal markers. the data emphasize the influence of temperature on cellular events of phagocytosis and pathogenesis of GBS diseases.UERJ Inst Biol Roberto Alcantara Gomes, Dept Biol Celular, BR-20550013 Rio de Janeiro, BrazilUniv Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Ultraestrutura Celular Hertha Meyer, BR-21941 Rio de Janeiro, BrazilUniv Estado Rio de Janeiro, Fac Ciencias Med, Disciplina Microbiol & Imunol, BR-20551030 Rio de Janeiro, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilWeb of ScienceSpandidos Publ LtdUniversidade do Estado do Rio de Janeiro (UERJ)Universidade Federal do Rio de Janeiro (UFRJ)Universidade Federal de São Paulo (UNIFESP)Freitas Lione, Viviane OliveiraBittencourt Dos Santos, Michelle HanthequesteUlisses Carvalho, Tecia MariaHirata, RaphaelMattos-Guaraldi, Ana LuizaMortara, Renato Arruda [UNIFESP]Nagao, Prescilla Emy2016-01-24T14:05:30Z2016-01-24T14:05:30Z2010-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion511-516http://dx.doi.org/10.3892/ijmm_00000493International Journal of Molecular Medicine. Athens: Spandidos Publ Ltd, v. 26, n. 4, p. 511-516, 2010.10.3892/ijmm_000004931107-3756http://repositorio.unifesp.br/handle/11600/32937WOS:000281917800010engInternational Journal of Molecular Medicineinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2016-01-24T12:05:30Zoai:repositorio.unifesp.br/:11600/32937Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652016-01-24T12:05:30Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Fever temperature enhances mechanisms of survival of Streptococcus agalactiae within human endothelial cells |
title |
Fever temperature enhances mechanisms of survival of Streptococcus agalactiae within human endothelial cells |
spellingShingle |
Fever temperature enhances mechanisms of survival of Streptococcus agalactiae within human endothelial cells Freitas Lione, Viviane Oliveira Streptococcus ugalactiae growth temperature endothelial cells fusion lysosomes-phagocytic vacuoles |
title_short |
Fever temperature enhances mechanisms of survival of Streptococcus agalactiae within human endothelial cells |
title_full |
Fever temperature enhances mechanisms of survival of Streptococcus agalactiae within human endothelial cells |
title_fullStr |
Fever temperature enhances mechanisms of survival of Streptococcus agalactiae within human endothelial cells |
title_full_unstemmed |
Fever temperature enhances mechanisms of survival of Streptococcus agalactiae within human endothelial cells |
title_sort |
Fever temperature enhances mechanisms of survival of Streptococcus agalactiae within human endothelial cells |
author |
Freitas Lione, Viviane Oliveira |
author_facet |
Freitas Lione, Viviane Oliveira Bittencourt Dos Santos, Michelle Hanthequeste Ulisses Carvalho, Tecia Maria Hirata, Raphael Mattos-Guaraldi, Ana Luiza Mortara, Renato Arruda [UNIFESP] Nagao, Prescilla Emy |
author_role |
author |
author2 |
Bittencourt Dos Santos, Michelle Hanthequeste Ulisses Carvalho, Tecia Maria Hirata, Raphael Mattos-Guaraldi, Ana Luiza Mortara, Renato Arruda [UNIFESP] Nagao, Prescilla Emy |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Estado do Rio de Janeiro (UERJ) Universidade Federal do Rio de Janeiro (UFRJ) Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Freitas Lione, Viviane Oliveira Bittencourt Dos Santos, Michelle Hanthequeste Ulisses Carvalho, Tecia Maria Hirata, Raphael Mattos-Guaraldi, Ana Luiza Mortara, Renato Arruda [UNIFESP] Nagao, Prescilla Emy |
dc.subject.por.fl_str_mv |
Streptococcus ugalactiae growth temperature endothelial cells fusion lysosomes-phagocytic vacuoles |
topic |
Streptococcus ugalactiae growth temperature endothelial cells fusion lysosomes-phagocytic vacuoles |
description |
Group B streptococci (GBS) are the most common cause of pneumonia and sepsis during the neonatal period. However, the pathogenesis of invasive infection is poorly understood. We investigated the ability of GBS grown at 37 C and 40 C to adhere and invade human umbilical vein endothelial cells (HUVECs) at different periods of incubation (0, 0.5, 1, 2, 18 and 24 h). All strains tested, except strain 88641-vagina survived for 24 h in the intracellular environment at 40 degrees C. for serotype III grown at 40 degrees C, both strains (80340-vagina and 90356-liquor) showed increased adherence and intracellular survival when compared to bacteria grown at 37 degrees C (P<0.01). GBS serotype V strains (88641-vagina and 90186-blood) showed ability to survive inside HUVECs until 2 and 24 h post-infection at 40 degrees C and 37 degrees C, respectively (P<0.01). Influence of growth temperature in bacterial interaction with endothelial cells was partially dependent of serotypes and the clinical origin of strains. Serotypes III and V strains grown at both temperatures remained viable within acidic endothelial vacuoles which acquired Rab7 and LAMP-1 endosomal markers. the data emphasize the influence of temperature on cellular events of phagocytosis and pathogenesis of GBS diseases. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-10-01 2016-01-24T14:05:30Z 2016-01-24T14:05:30Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3892/ijmm_00000493 International Journal of Molecular Medicine. Athens: Spandidos Publ Ltd, v. 26, n. 4, p. 511-516, 2010. 10.3892/ijmm_00000493 1107-3756 http://repositorio.unifesp.br/handle/11600/32937 WOS:000281917800010 |
url |
http://dx.doi.org/10.3892/ijmm_00000493 http://repositorio.unifesp.br/handle/11600/32937 |
identifier_str_mv |
International Journal of Molecular Medicine. Athens: Spandidos Publ Ltd, v. 26, n. 4, p. 511-516, 2010. 10.3892/ijmm_00000493 1107-3756 WOS:000281917800010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Molecular Medicine |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
511-516 |
dc.publisher.none.fl_str_mv |
Spandidos Publ Ltd |
publisher.none.fl_str_mv |
Spandidos Publ Ltd |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268395740200960 |