Interferon-γ inhibits group B Streptococcus survival within human endothelial cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Memórias do Instituto Oswaldo Cruz |
| Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000700940 |
Resumo: | Endothelial dysfunction is a major component of the pathophysiology of septicaemic group B Streptococcus (GBS) infections. Although cytokines have been shown to activate human umbilical vein endothelial cells (HUVECs), the capacity of interferon (IFN)-γ to enhance the microbicidal activity of HUVECs against GBS has not been studied. We report that the viability of intracellular bacteria was reduced in HUVECs activated by IFN-γ. Enhanced fusion of lysosomes with bacteria-containing vacuoles was observed by acid phosphatase and the colocalisation of Rab-5, Rab-7 and lysosomal-associated membrane protein-1 with GBS in IFN-γ-activated HUVECs. IFN-γ resulted in an enhancement of the phagosome maturation process in HUVECs, improving the capacity to control the intracellular survival of GBS. |
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Interferon-γ inhibits group B Streptococcus survival within human endothelial cellsgroup B StreptococcusIFN-γsurvivalEndothelial dysfunction is a major component of the pathophysiology of septicaemic group B Streptococcus (GBS) infections. Although cytokines have been shown to activate human umbilical vein endothelial cells (HUVECs), the capacity of interferon (IFN)-γ to enhance the microbicidal activity of HUVECs against GBS has not been studied. We report that the viability of intracellular bacteria was reduced in HUVECs activated by IFN-γ. Enhanced fusion of lysosomes with bacteria-containing vacuoles was observed by acid phosphatase and the colocalisation of Rab-5, Rab-7 and lysosomal-associated membrane protein-1 with GBS in IFN-γ-activated HUVECs. IFN-γ resulted in an enhancement of the phagosome maturation process in HUVECs, improving the capacity to control the intracellular survival of GBS.Instituto Oswaldo Cruz, Ministério da Saúde2014-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000700940Memórias do Instituto Oswaldo Cruz v.109 n.7 2014reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-0276140201info:eu-repo/semantics/openAccessLione,Viviane de Oliveira FreitasSantos,Michelle Hanthequeste Bittencourt dosOliveira,Jessica Silva Santos deMattos-Guaraldi,Ana LuizaNagao,Prescilla Emyeng2020-04-25T17:51:48Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:19:53.827Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
| dc.title.none.fl_str_mv |
Interferon-γ inhibits group B Streptococcus survival within human endothelial cells |
| title |
Interferon-γ inhibits group B Streptococcus survival within human endothelial cells |
| spellingShingle |
Interferon-γ inhibits group B Streptococcus survival within human endothelial cells Lione,Viviane de Oliveira Freitas group B Streptococcus IFN-γ survival |
| title_short |
Interferon-γ inhibits group B Streptococcus survival within human endothelial cells |
| title_full |
Interferon-γ inhibits group B Streptococcus survival within human endothelial cells |
| title_fullStr |
Interferon-γ inhibits group B Streptococcus survival within human endothelial cells |
| title_full_unstemmed |
Interferon-γ inhibits group B Streptococcus survival within human endothelial cells |
| title_sort |
Interferon-γ inhibits group B Streptococcus survival within human endothelial cells |
| author |
Lione,Viviane de Oliveira Freitas |
| author_facet |
Lione,Viviane de Oliveira Freitas Santos,Michelle Hanthequeste Bittencourt dos Oliveira,Jessica Silva Santos de Mattos-Guaraldi,Ana Luiza Nagao,Prescilla Emy |
| author_role |
author |
| author2 |
Santos,Michelle Hanthequeste Bittencourt dos Oliveira,Jessica Silva Santos de Mattos-Guaraldi,Ana Luiza Nagao,Prescilla Emy |
| author2_role |
author author author author |
| dc.contributor.author.fl_str_mv |
Lione,Viviane de Oliveira Freitas Santos,Michelle Hanthequeste Bittencourt dos Oliveira,Jessica Silva Santos de Mattos-Guaraldi,Ana Luiza Nagao,Prescilla Emy |
| dc.subject.por.fl_str_mv |
group B Streptococcus IFN-γ survival |
| topic |
group B Streptococcus IFN-γ survival |
| dc.description.none.fl_txt_mv |
Endothelial dysfunction is a major component of the pathophysiology of septicaemic group B Streptococcus (GBS) infections. Although cytokines have been shown to activate human umbilical vein endothelial cells (HUVECs), the capacity of interferon (IFN)-γ to enhance the microbicidal activity of HUVECs against GBS has not been studied. We report that the viability of intracellular bacteria was reduced in HUVECs activated by IFN-γ. Enhanced fusion of lysosomes with bacteria-containing vacuoles was observed by acid phosphatase and the colocalisation of Rab-5, Rab-7 and lysosomal-associated membrane protein-1 with GBS in IFN-γ-activated HUVECs. IFN-γ resulted in an enhancement of the phagosome maturation process in HUVECs, improving the capacity to control the intracellular survival of GBS. |
| description |
Endothelial dysfunction is a major component of the pathophysiology of septicaemic group B Streptococcus (GBS) infections. Although cytokines have been shown to activate human umbilical vein endothelial cells (HUVECs), the capacity of interferon (IFN)-γ to enhance the microbicidal activity of HUVECs against GBS has not been studied. We report that the viability of intracellular bacteria was reduced in HUVECs activated by IFN-γ. Enhanced fusion of lysosomes with bacteria-containing vacuoles was observed by acid phosphatase and the colocalisation of Rab-5, Rab-7 and lysosomal-associated membrane protein-1 with GBS in IFN-γ-activated HUVECs. IFN-γ resulted in an enhancement of the phagosome maturation process in HUVECs, improving the capacity to control the intracellular survival of GBS. |
| publishDate |
2014 |
| dc.date.none.fl_str_mv |
2014-11-01 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000700940 |
| url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000700940 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
10.1590/0074-0276140201 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
text/html |
| dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
| publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
| dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.109 n.7 2014 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
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Memórias do Instituto Oswaldo Cruz |
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Memórias do Instituto Oswaldo Cruz |
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Fundação Oswaldo Cruz |
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FIOCRUZ |
| institution |
FIOCRUZ |
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Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
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1669937716964360192 |