MOLECULAR-STRUCTURE AND EXPRESSION OF RAT BRADYKININ B2 RECEPTOR GENE - EVIDENCE FOR ALTERNATIVE SPLICING
Autor(a) principal: | |
---|---|
Data de Publicação: | 1994 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://www.jbc.org/content/269/43/26920.abstract http://repositorio.unifesp.br/handle/11600/44201 |
Resumo: | Bradykinin is a potent vasodilatory peptide hormone involved in a broad range of physiological actions. While it acts through at least two types of receptors which are named B1 and B2, most of its effects are mediated via activation of the B2 receptor. The gene for this receptor was isolated hom a rat genomic library and shown to span more than 28 kilobases, including four introns. The relative positions of the exons were mapped and all exons, intron-exon boundaries, and 5'- and 3'-flanking regions were sequenced. While the 5'-untranslated region of the mRNA is distributed on all four exons, the coding and the 3'-untranslated region are located entirely on the fourth exon. Characterization of the region upstream to the transcriptional start site detected by primer extension analysis shows that the bradykinin B2 receptor promoter contains no typical TATA or CCAAT boxes. Nevertheless, the promoter sequence was shown to be functional in NG108-15 cells transfected with a construct bearing 1.1 kilobases of 5'-flanking sequence fused to a luciferase reporter gene. Reverse transcription-polymerase chain reaction analysis detected two different bradykinin B2 receptor mRNAs containing or lacking exon 3 in all rat tissues tested, providing evidence for alternative splicing of the 5'-untranslated sequence. |
id |
UFSP_7fa08a9ecc3cd665649335a1041f2392 |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br/:11600/44201 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
MOLECULAR-STRUCTURE AND EXPRESSION OF RAT BRADYKININ B2 RECEPTOR GENE - EVIDENCE FOR ALTERNATIVE SPLICINGBradykinin is a potent vasodilatory peptide hormone involved in a broad range of physiological actions. While it acts through at least two types of receptors which are named B1 and B2, most of its effects are mediated via activation of the B2 receptor. The gene for this receptor was isolated hom a rat genomic library and shown to span more than 28 kilobases, including four introns. The relative positions of the exons were mapped and all exons, intron-exon boundaries, and 5'- and 3'-flanking regions were sequenced. While the 5'-untranslated region of the mRNA is distributed on all four exons, the coding and the 3'-untranslated region are located entirely on the fourth exon. Characterization of the region upstream to the transcriptional start site detected by primer extension analysis shows that the bradykinin B2 receptor promoter contains no typical TATA or CCAAT boxes. Nevertheless, the promoter sequence was shown to be functional in NG108-15 cells transfected with a construct bearing 1.1 kilobases of 5'-flanking sequence fused to a luciferase reporter gene. Reverse transcription-polymerase chain reaction analysis detected two different bradykinin B2 receptor mRNAs containing or lacking exon 3 in all rat tissues tested, providing evidence for alternative splicing of the 5'-untranslated sequence.MAX DELBRUCK CTR MOLEC MED,D-13122 BERLIN,GERMANYESCOLA PAULISTA MED,DEPT BIOPHYS,BR-04034970 SAO PAULO,BRAZILESCOLA PAULISTA MED,DEPT BIOPHYS,BR-04034970 SAO PAULO,BRAZILWeb of ScienceAmer Soc Biochemistry Molecular Biology IncMAX DELBRUCK CTR MOLEC MEDUniversidade Federal de São Paulo (UNIFESP)Pesquero, João Bosco [UNIFESP]Lindsey, Charles Julian [UNIFESP]Zeh, K.Paiva, Antonio Cechelli de Mattos [UNIFESP]Ganten, D.Bader, Michael [UNIFESP]2018-06-15T17:53:04Z2018-06-15T17:53:04Z1994-10-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion26920-26925http://www.jbc.org/content/269/43/26920.abstractJournal Of Biological Chemistry. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 269, n. 43, p. 26920-26925, 1994.0021-9258http://repositorio.unifesp.br/handle/11600/44201WOS:A1994PQ93100053engJournal Of Biological Chemistryinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-05-02T13:55:49Zoai:repositorio.unifesp.br/:11600/44201Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-05-02T13:55:49Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
MOLECULAR-STRUCTURE AND EXPRESSION OF RAT BRADYKININ B2 RECEPTOR GENE - EVIDENCE FOR ALTERNATIVE SPLICING |
title |
MOLECULAR-STRUCTURE AND EXPRESSION OF RAT BRADYKININ B2 RECEPTOR GENE - EVIDENCE FOR ALTERNATIVE SPLICING |
spellingShingle |
MOLECULAR-STRUCTURE AND EXPRESSION OF RAT BRADYKININ B2 RECEPTOR GENE - EVIDENCE FOR ALTERNATIVE SPLICING Pesquero, João Bosco [UNIFESP] |
title_short |
MOLECULAR-STRUCTURE AND EXPRESSION OF RAT BRADYKININ B2 RECEPTOR GENE - EVIDENCE FOR ALTERNATIVE SPLICING |
title_full |
MOLECULAR-STRUCTURE AND EXPRESSION OF RAT BRADYKININ B2 RECEPTOR GENE - EVIDENCE FOR ALTERNATIVE SPLICING |
title_fullStr |
MOLECULAR-STRUCTURE AND EXPRESSION OF RAT BRADYKININ B2 RECEPTOR GENE - EVIDENCE FOR ALTERNATIVE SPLICING |
title_full_unstemmed |
MOLECULAR-STRUCTURE AND EXPRESSION OF RAT BRADYKININ B2 RECEPTOR GENE - EVIDENCE FOR ALTERNATIVE SPLICING |
title_sort |
MOLECULAR-STRUCTURE AND EXPRESSION OF RAT BRADYKININ B2 RECEPTOR GENE - EVIDENCE FOR ALTERNATIVE SPLICING |
author |
Pesquero, João Bosco [UNIFESP] |
author_facet |
Pesquero, João Bosco [UNIFESP] Lindsey, Charles Julian [UNIFESP] Zeh, K. Paiva, Antonio Cechelli de Mattos [UNIFESP] Ganten, D. Bader, Michael [UNIFESP] |
author_role |
author |
author2 |
Lindsey, Charles Julian [UNIFESP] Zeh, K. Paiva, Antonio Cechelli de Mattos [UNIFESP] Ganten, D. Bader, Michael [UNIFESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
MAX DELBRUCK CTR MOLEC MED Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Pesquero, João Bosco [UNIFESP] Lindsey, Charles Julian [UNIFESP] Zeh, K. Paiva, Antonio Cechelli de Mattos [UNIFESP] Ganten, D. Bader, Michael [UNIFESP] |
description |
Bradykinin is a potent vasodilatory peptide hormone involved in a broad range of physiological actions. While it acts through at least two types of receptors which are named B1 and B2, most of its effects are mediated via activation of the B2 receptor. The gene for this receptor was isolated hom a rat genomic library and shown to span more than 28 kilobases, including four introns. The relative positions of the exons were mapped and all exons, intron-exon boundaries, and 5'- and 3'-flanking regions were sequenced. While the 5'-untranslated region of the mRNA is distributed on all four exons, the coding and the 3'-untranslated region are located entirely on the fourth exon. Characterization of the region upstream to the transcriptional start site detected by primer extension analysis shows that the bradykinin B2 receptor promoter contains no typical TATA or CCAAT boxes. Nevertheless, the promoter sequence was shown to be functional in NG108-15 cells transfected with a construct bearing 1.1 kilobases of 5'-flanking sequence fused to a luciferase reporter gene. Reverse transcription-polymerase chain reaction analysis detected two different bradykinin B2 receptor mRNAs containing or lacking exon 3 in all rat tissues tested, providing evidence for alternative splicing of the 5'-untranslated sequence. |
publishDate |
1994 |
dc.date.none.fl_str_mv |
1994-10-28 2018-06-15T17:53:04Z 2018-06-15T17:53:04Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.jbc.org/content/269/43/26920.abstract Journal Of Biological Chemistry. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 269, n. 43, p. 26920-26925, 1994. 0021-9258 http://repositorio.unifesp.br/handle/11600/44201 WOS:A1994PQ93100053 |
url |
http://www.jbc.org/content/269/43/26920.abstract http://repositorio.unifesp.br/handle/11600/44201 |
identifier_str_mv |
Journal Of Biological Chemistry. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 269, n. 43, p. 26920-26925, 1994. 0021-9258 WOS:A1994PQ93100053 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Biological Chemistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
26920-26925 |
dc.publisher.none.fl_str_mv |
Amer Soc Biochemistry Molecular Biology Inc |
publisher.none.fl_str_mv |
Amer Soc Biochemistry Molecular Biology Inc |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268342407528448 |