MOLECULAR-STRUCTURE AND EXPRESSION OF RAT BRADYKININ B2 RECEPTOR GENE - EVIDENCE FOR ALTERNATIVE SPLICING

Detalhes bibliográficos
Autor(a) principal: Pesquero, João Bosco [UNIFESP]
Data de Publicação: 1994
Outros Autores: Lindsey, Charles Julian [UNIFESP], Zeh, K., Paiva, Antonio Cechelli de Mattos [UNIFESP], Ganten, D., Bader, Michael [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://www.jbc.org/content/269/43/26920.abstract
http://repositorio.unifesp.br/handle/11600/44201
Resumo: Bradykinin is a potent vasodilatory peptide hormone involved in a broad range of physiological actions. While it acts through at least two types of receptors which are named B1 and B2, most of its effects are mediated via activation of the B2 receptor. The gene for this receptor was isolated hom a rat genomic library and shown to span more than 28 kilobases, including four introns. The relative positions of the exons were mapped and all exons, intron-exon boundaries, and 5'- and 3'-flanking regions were sequenced. While the 5'-untranslated region of the mRNA is distributed on all four exons, the coding and the 3'-untranslated region are located entirely on the fourth exon. Characterization of the region upstream to the transcriptional start site detected by primer extension analysis shows that the bradykinin B2 receptor promoter contains no typical TATA or CCAAT boxes. Nevertheless, the promoter sequence was shown to be functional in NG108-15 cells transfected with a construct bearing 1.1 kilobases of 5'-flanking sequence fused to a luciferase reporter gene. Reverse transcription-polymerase chain reaction analysis detected two different bradykinin B2 receptor mRNAs containing or lacking exon 3 in all rat tissues tested, providing evidence for alternative splicing of the 5'-untranslated sequence.
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spelling MOLECULAR-STRUCTURE AND EXPRESSION OF RAT BRADYKININ B2 RECEPTOR GENE - EVIDENCE FOR ALTERNATIVE SPLICINGBradykinin is a potent vasodilatory peptide hormone involved in a broad range of physiological actions. While it acts through at least two types of receptors which are named B1 and B2, most of its effects are mediated via activation of the B2 receptor. The gene for this receptor was isolated hom a rat genomic library and shown to span more than 28 kilobases, including four introns. The relative positions of the exons were mapped and all exons, intron-exon boundaries, and 5'- and 3'-flanking regions were sequenced. While the 5'-untranslated region of the mRNA is distributed on all four exons, the coding and the 3'-untranslated region are located entirely on the fourth exon. Characterization of the region upstream to the transcriptional start site detected by primer extension analysis shows that the bradykinin B2 receptor promoter contains no typical TATA or CCAAT boxes. Nevertheless, the promoter sequence was shown to be functional in NG108-15 cells transfected with a construct bearing 1.1 kilobases of 5'-flanking sequence fused to a luciferase reporter gene. Reverse transcription-polymerase chain reaction analysis detected two different bradykinin B2 receptor mRNAs containing or lacking exon 3 in all rat tissues tested, providing evidence for alternative splicing of the 5'-untranslated sequence.MAX DELBRUCK CTR MOLEC MED,D-13122 BERLIN,GERMANYESCOLA PAULISTA MED,DEPT BIOPHYS,BR-04034970 SAO PAULO,BRAZILESCOLA PAULISTA MED,DEPT BIOPHYS,BR-04034970 SAO PAULO,BRAZILWeb of ScienceAmer Soc Biochemistry Molecular Biology IncMAX DELBRUCK CTR MOLEC MEDUniversidade Federal de São Paulo (UNIFESP)Pesquero, João Bosco [UNIFESP]Lindsey, Charles Julian [UNIFESP]Zeh, K.Paiva, Antonio Cechelli de Mattos [UNIFESP]Ganten, D.Bader, Michael [UNIFESP]2018-06-15T17:53:04Z2018-06-15T17:53:04Z1994-10-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion26920-26925http://www.jbc.org/content/269/43/26920.abstractJournal Of Biological Chemistry. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 269, n. 43, p. 26920-26925, 1994.0021-9258http://repositorio.unifesp.br/handle/11600/44201WOS:A1994PQ93100053engJournal Of Biological Chemistryinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-05-02T13:55:49Zoai:repositorio.unifesp.br/:11600/44201Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-05-02T13:55:49Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv MOLECULAR-STRUCTURE AND EXPRESSION OF RAT BRADYKININ B2 RECEPTOR GENE - EVIDENCE FOR ALTERNATIVE SPLICING
title MOLECULAR-STRUCTURE AND EXPRESSION OF RAT BRADYKININ B2 RECEPTOR GENE - EVIDENCE FOR ALTERNATIVE SPLICING
spellingShingle MOLECULAR-STRUCTURE AND EXPRESSION OF RAT BRADYKININ B2 RECEPTOR GENE - EVIDENCE FOR ALTERNATIVE SPLICING
Pesquero, João Bosco [UNIFESP]
title_short MOLECULAR-STRUCTURE AND EXPRESSION OF RAT BRADYKININ B2 RECEPTOR GENE - EVIDENCE FOR ALTERNATIVE SPLICING
title_full MOLECULAR-STRUCTURE AND EXPRESSION OF RAT BRADYKININ B2 RECEPTOR GENE - EVIDENCE FOR ALTERNATIVE SPLICING
title_fullStr MOLECULAR-STRUCTURE AND EXPRESSION OF RAT BRADYKININ B2 RECEPTOR GENE - EVIDENCE FOR ALTERNATIVE SPLICING
title_full_unstemmed MOLECULAR-STRUCTURE AND EXPRESSION OF RAT BRADYKININ B2 RECEPTOR GENE - EVIDENCE FOR ALTERNATIVE SPLICING
title_sort MOLECULAR-STRUCTURE AND EXPRESSION OF RAT BRADYKININ B2 RECEPTOR GENE - EVIDENCE FOR ALTERNATIVE SPLICING
author Pesquero, João Bosco [UNIFESP]
author_facet Pesquero, João Bosco [UNIFESP]
Lindsey, Charles Julian [UNIFESP]
Zeh, K.
Paiva, Antonio Cechelli de Mattos [UNIFESP]
Ganten, D.
Bader, Michael [UNIFESP]
author_role author
author2 Lindsey, Charles Julian [UNIFESP]
Zeh, K.
Paiva, Antonio Cechelli de Mattos [UNIFESP]
Ganten, D.
Bader, Michael [UNIFESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv MAX DELBRUCK CTR MOLEC MED
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Pesquero, João Bosco [UNIFESP]
Lindsey, Charles Julian [UNIFESP]
Zeh, K.
Paiva, Antonio Cechelli de Mattos [UNIFESP]
Ganten, D.
Bader, Michael [UNIFESP]
description Bradykinin is a potent vasodilatory peptide hormone involved in a broad range of physiological actions. While it acts through at least two types of receptors which are named B1 and B2, most of its effects are mediated via activation of the B2 receptor. The gene for this receptor was isolated hom a rat genomic library and shown to span more than 28 kilobases, including four introns. The relative positions of the exons were mapped and all exons, intron-exon boundaries, and 5'- and 3'-flanking regions were sequenced. While the 5'-untranslated region of the mRNA is distributed on all four exons, the coding and the 3'-untranslated region are located entirely on the fourth exon. Characterization of the region upstream to the transcriptional start site detected by primer extension analysis shows that the bradykinin B2 receptor promoter contains no typical TATA or CCAAT boxes. Nevertheless, the promoter sequence was shown to be functional in NG108-15 cells transfected with a construct bearing 1.1 kilobases of 5'-flanking sequence fused to a luciferase reporter gene. Reverse transcription-polymerase chain reaction analysis detected two different bradykinin B2 receptor mRNAs containing or lacking exon 3 in all rat tissues tested, providing evidence for alternative splicing of the 5'-untranslated sequence.
publishDate 1994
dc.date.none.fl_str_mv 1994-10-28
2018-06-15T17:53:04Z
2018-06-15T17:53:04Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.jbc.org/content/269/43/26920.abstract
Journal Of Biological Chemistry. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 269, n. 43, p. 26920-26925, 1994.
0021-9258
http://repositorio.unifesp.br/handle/11600/44201
WOS:A1994PQ93100053
url http://www.jbc.org/content/269/43/26920.abstract
http://repositorio.unifesp.br/handle/11600/44201
identifier_str_mv Journal Of Biological Chemistry. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 269, n. 43, p. 26920-26925, 1994.
0021-9258
WOS:A1994PQ93100053
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal Of Biological Chemistry
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 26920-26925
dc.publisher.none.fl_str_mv Amer Soc Biochemistry Molecular Biology Inc
publisher.none.fl_str_mv Amer Soc Biochemistry Molecular Biology Inc
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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