Hypothalamic transcriptional expression of the kis-speptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypes
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/51409 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322017000800510&lng=en&nrm=iso&tlng=en |
Resumo: | OBJECTIVES: Polycystic ovary syndrome is a heterogeneous endocrine disorder that affects reproductive-age women. The mechanisms underlying the endocrine heterogeneity and neuroendocrinology of polycystic ovary syndrome are still unclear. In this study, we investigated the expression of the kisspeptin system and gonadotropin-releasing hormone pulse regulators in the hypothalamus as well as factors related to luteinizing hormone secretion in the pituitary of polycystic ovary syndrome rat models induced by testosterone or estradiol. METHODS: A single injection of testosterone propionate (1.25 mg) (n= 10) or estradiol benzoate (0.5 mg) (n= 10) was administered to female rats at 2 days of age to induce experimental polycystic ovary syndrome. Controls were injected with a vehicle (n= 10). Animals were euthanized at 90-94 days of age, and the hypothalamus and pituitary gland were used for gene expression analysis. RESULTS: Rats exposed to testosterone exhibited increased transcriptional expression of the androgen receptor and estrogen receptor-b and reduced expression of kisspeptin in the hypothalamus. However, rats exposed to estradiol did not show any significant changes in hormone levels relative to controls but exhibited hypothalamic downregulation of kisspeptin, tachykinin 3 and estrogen receptor-a genes and upregulation of the gene that encodes the kisspeptin receptor. CONCLUSIONS: Testosterone-and estradiol-exposed rats with different endocrine phenotypes showed differential transcriptional expression of members of the kisspeptin system and sex steroid receptors in the hypothalamus. These differences might account for the different endocrine phenotypes found in testosteroneand estradiol-induced polycystic ovary syndrome rats. |
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Marcondes, Rodrigo RodriguesCarvalho, Katia CandidoGiannocco, Gisele [UNIFESP]Duarte, Daniele CoelhoGarcia, NataliaSoares-Junior, Jose MariaCotrim Guerreiro da Silva, Ismael Dale [UNIFESP]Maliqueo, ManuelBaracat, Edmund ChadaRosa Maciel, Gustavo Arantes2019-08-19T11:49:48Z2019-08-19T11:49:48Z2017Clinics. Sao Paulo, v. 72, n. 8, p. 510-514, 2017.1807-5932http://repositorio.unifesp.br/handle/11600/51409http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322017000800510&lng=en&nrm=iso&tlng=enWOS000411803900009.pdfS1807-5932201700080051010.6061/clinics/2017(08)09WOS:000411803900009OBJECTIVES: Polycystic ovary syndrome is a heterogeneous endocrine disorder that affects reproductive-age women. The mechanisms underlying the endocrine heterogeneity and neuroendocrinology of polycystic ovary syndrome are still unclear. In this study, we investigated the expression of the kisspeptin system and gonadotropin-releasing hormone pulse regulators in the hypothalamus as well as factors related to luteinizing hormone secretion in the pituitary of polycystic ovary syndrome rat models induced by testosterone or estradiol. METHODS: A single injection of testosterone propionate (1.25 mg) (n= 10) or estradiol benzoate (0.5 mg) (n= 10) was administered to female rats at 2 days of age to induce experimental polycystic ovary syndrome. Controls were injected with a vehicle (n= 10). Animals were euthanized at 90-94 days of age, and the hypothalamus and pituitary gland were used for gene expression analysis. RESULTS: Rats exposed to testosterone exhibited increased transcriptional expression of the androgen receptor and estrogen receptor-b and reduced expression of kisspeptin in the hypothalamus. However, rats exposed to estradiol did not show any significant changes in hormone levels relative to controls but exhibited hypothalamic downregulation of kisspeptin, tachykinin 3 and estrogen receptor-a genes and upregulation of the gene that encodes the kisspeptin receptor. CONCLUSIONS: Testosterone-and estradiol-exposed rats with different endocrine phenotypes showed differential transcriptional expression of members of the kisspeptin system and sex steroid receptors in the hypothalamus. These differences might account for the different endocrine phenotypes found in testosteroneand estradiol-induced polycystic ovary syndrome rats.Fundação de Amparo Pesquisa do Estado de São Paulo (FAPESP) -BrazilMaster's degree scholarship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) -BrazilUniv São Paulo, Fac Med FMUSP, Disciplina Ginecol, Lab Ginecol Estrutural & Mol LIM 58, São Paulo, SP, BrazilUniv Fed São Paulo, Dept Med, Lab Endocrinol Mol & Translac, São Paulo, BrazilUniv Fed São Paulo, Escola Paulista Med, Dept Ginecol, Lab Ginecol Mol & Proteom, São Paulo, SP, BrazilUniv Chile, West Div, Dept Med, Endocrinol & Metab Lab, Santiago, ChileUniv Fed São Paulo, Dept Med, Lab Endocrinol Mol & Translac, São Paulo, BrazilUniv Fed São Paulo, Escola Paulista Med, Dept Ginecol, Lab Ginecol Mol & Proteom, São Paulo, SP, BrazilFAPESP: 2010/17417-3FAPESP: 2013/12830-8CNPq: 134694/2010-4Web of Science510-514engHospital Clinicas, Univ Sao PauloPolycystic Ovary SyndromeHypothalamusAnimal ModelsKisspeptinTestosteroneHypothalamic transcriptional expression of the kis-speptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000411803900009.pdfapplication/pdf529980${dspace.ui.url}/bitstream/11600/51409/1/WOS000411803900009.pdf3615dffc5557c4fff1aada2b093950bbMD51open accessTEXTWOS000411803900009.pdf.txtWOS000411803900009.pdf.txtExtracted texttext/plain22976${dspace.ui.url}/bitstream/11600/51409/2/WOS000411803900009.pdf.txt2fe544295d5344dd3dd2bdd93bff783aMD52open accessTHUMBNAILWOS000411803900009.pdf.jpgWOS000411803900009.pdf.jpgIM Thumbnailimage/jpeg9506${dspace.ui.url}/bitstream/11600/51409/4/WOS000411803900009.pdf.jpga78bcb10fc1699a5cb60c013fe7503cdMD54open access11600/514092022-08-01 18:17:54.752open accessoai:repositorio.unifesp.br:11600/51409Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:18:02.337608Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Hypothalamic transcriptional expression of the kis-speptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypes |
title |
Hypothalamic transcriptional expression of the kis-speptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypes |
spellingShingle |
Hypothalamic transcriptional expression of the kis-speptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypes Marcondes, Rodrigo Rodrigues Polycystic Ovary Syndrome Hypothalamus Animal Models Kisspeptin Testosterone |
title_short |
Hypothalamic transcriptional expression of the kis-speptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypes |
title_full |
Hypothalamic transcriptional expression of the kis-speptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypes |
title_fullStr |
Hypothalamic transcriptional expression of the kis-speptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypes |
title_full_unstemmed |
Hypothalamic transcriptional expression of the kis-speptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypes |
title_sort |
Hypothalamic transcriptional expression of the kis-speptin system and sex steroid receptors differs among polycystic ovary syndrome rat models with different endocrine phenotypes |
author |
Marcondes, Rodrigo Rodrigues |
author_facet |
Marcondes, Rodrigo Rodrigues Carvalho, Katia Candido Giannocco, Gisele [UNIFESP] Duarte, Daniele Coelho Garcia, Natalia Soares-Junior, Jose Maria Cotrim Guerreiro da Silva, Ismael Dale [UNIFESP] Maliqueo, Manuel Baracat, Edmund Chada Rosa Maciel, Gustavo Arantes |
author_role |
author |
author2 |
Carvalho, Katia Candido Giannocco, Gisele [UNIFESP] Duarte, Daniele Coelho Garcia, Natalia Soares-Junior, Jose Maria Cotrim Guerreiro da Silva, Ismael Dale [UNIFESP] Maliqueo, Manuel Baracat, Edmund Chada Rosa Maciel, Gustavo Arantes |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Marcondes, Rodrigo Rodrigues Carvalho, Katia Candido Giannocco, Gisele [UNIFESP] Duarte, Daniele Coelho Garcia, Natalia Soares-Junior, Jose Maria Cotrim Guerreiro da Silva, Ismael Dale [UNIFESP] Maliqueo, Manuel Baracat, Edmund Chada Rosa Maciel, Gustavo Arantes |
dc.subject.eng.fl_str_mv |
Polycystic Ovary Syndrome Hypothalamus Animal Models Kisspeptin Testosterone |
topic |
Polycystic Ovary Syndrome Hypothalamus Animal Models Kisspeptin Testosterone |
description |
OBJECTIVES: Polycystic ovary syndrome is a heterogeneous endocrine disorder that affects reproductive-age women. The mechanisms underlying the endocrine heterogeneity and neuroendocrinology of polycystic ovary syndrome are still unclear. In this study, we investigated the expression of the kisspeptin system and gonadotropin-releasing hormone pulse regulators in the hypothalamus as well as factors related to luteinizing hormone secretion in the pituitary of polycystic ovary syndrome rat models induced by testosterone or estradiol. METHODS: A single injection of testosterone propionate (1.25 mg) (n= 10) or estradiol benzoate (0.5 mg) (n= 10) was administered to female rats at 2 days of age to induce experimental polycystic ovary syndrome. Controls were injected with a vehicle (n= 10). Animals were euthanized at 90-94 days of age, and the hypothalamus and pituitary gland were used for gene expression analysis. RESULTS: Rats exposed to testosterone exhibited increased transcriptional expression of the androgen receptor and estrogen receptor-b and reduced expression of kisspeptin in the hypothalamus. However, rats exposed to estradiol did not show any significant changes in hormone levels relative to controls but exhibited hypothalamic downregulation of kisspeptin, tachykinin 3 and estrogen receptor-a genes and upregulation of the gene that encodes the kisspeptin receptor. CONCLUSIONS: Testosterone-and estradiol-exposed rats with different endocrine phenotypes showed differential transcriptional expression of members of the kisspeptin system and sex steroid receptors in the hypothalamus. These differences might account for the different endocrine phenotypes found in testosteroneand estradiol-induced polycystic ovary syndrome rats. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2019-08-19T11:49:48Z |
dc.date.available.fl_str_mv |
2019-08-19T11:49:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Clinics. Sao Paulo, v. 72, n. 8, p. 510-514, 2017. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/51409 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322017000800510&lng=en&nrm=iso&tlng=en |
dc.identifier.issn.none.fl_str_mv |
1807-5932 |
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WOS000411803900009.pdf |
dc.identifier.scielo.none.fl_str_mv |
S1807-59322017000800510 |
dc.identifier.doi.none.fl_str_mv |
10.6061/clinics/2017(08)09 |
dc.identifier.wos.none.fl_str_mv |
WOS:000411803900009 |
identifier_str_mv |
Clinics. Sao Paulo, v. 72, n. 8, p. 510-514, 2017. 1807-5932 WOS000411803900009.pdf S1807-59322017000800510 10.6061/clinics/2017(08)09 WOS:000411803900009 |
url |
http://repositorio.unifesp.br/handle/11600/51409 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322017000800510&lng=en&nrm=iso&tlng=en |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
510-514 |
dc.publisher.none.fl_str_mv |
Hospital Clinicas, Univ Sao Paulo |
publisher.none.fl_str_mv |
Hospital Clinicas, Univ Sao Paulo |
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reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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