Mycobacterium bovis: polymerase chain reaction identification in bovine Lymphonode biopsies and genotyping in isolates from Southeast Brazil by spolygotyping and restriction fragment length polymorphism

• Autor(a) principal: Zanini, Ms
• Data de Publicação: 2001
• Outros Autores: Moreira, Ec, Lopes, Mtp, Oliveira, Rs [UNIFESP], Leao, Sylvia Cardoso [UNIFESP], Fioravanti, Rl, Roxo, E, Zumarraga, M, Romano, Mi, Cataldi, A, Salas, Ce
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.1590/S0074-02762001000600012; http://repositorio.unifesp.br/handle/11600/1226
• topic: Mycobacterium bovis diagnosis; DNA polimorphism; thiocyanate guanidinium; Brazil

Diagnosis of the Mycobacterium tuberculosis complex by direct PCR of mediastinal lymphnode DNA and microbiological tests were compared in cattle suspicious of bearing tuberculous-like lesions detected during slaughter. The PCR procedure applied on DNA samples (n=54) obtained by adding alpha -casein into the thiocyanate extraction mix was positive in 70% of the samples. PCR confirmed the identification of 23 samples (100%) that grew in culture, 9 samples (60%) that failed to grow in culture, plus 6 (37.5%) samples that resulted in growth of bacterial contaminants. Genotyping by IS6110-RFLP and DR-spoligotyping analysis of seven samples revealed the presence of several polimorphisms. Seven of the isolates contained multiple copies of IS6110, thus defining the existence of five singular genotypes.