Effects of Anterior Thalamic Nucleus Deep Brain Stimulation in Chronic Epileptic Rats
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/37857 http://dx.doi.org/10.1371/journal.pone.0097618 |
Resumo: | Deep brain stimulation (DBS) has been investigated for the treatment of epilepsy. in rodents, an increase in the latency for the development of seizures and status epilepticus (SE) has been reported in different animal models but the consequences of delivering stimulation to chronic epileptic animals have not been extensively addressed. We study the effects of anterior thalamic nucleus (AN) stimulation at different current intensities in rats rendered epileptic following pilocarpine (Pilo) administration. Four months after Pilo-induced SE, chronic epileptic rats were bilaterally implanted with AN electrodes or had sham-surgery. Stimulation was delivered for 6 h/day, 5 days/week at 130 Hz, 90 mu sec. and either 100 mu A or 500 mu A. the frequency of spontaneous recurrent seizures in animals receiving stimulation was compared to that recorded in the preoperative period and in rats given sham treatment. To investigate the effects of DBS on hippocampal excitability, brain slices from animals receiving AN DBS or sham surgery were studied with electrophysiology. We found that rats treated with AN DBS at 100 mu A had a 52% non-significant reduction in the frequency of seizures as compared to sham-treated controls and 61% less seizures than at baseline. Animals given DBS at 500 mu A had 5.1 times more seizures than controls and a 2.8 fold increase in seizure rate as compared to preoperative values. in non-stimulated controls, the average frequency of seizures before and after surgery remained unaltered. in vitro recordings have shown that slices from animals previously given DBS at 100 mu A had a longer latency for the development of epileptiform activity, shorter and smaller DC shifts, and a smaller spike amplitude compared to non-stimulated controls. in contrast, a higher spike amplitude was recorded in slices from animals given AN DBS at 500 mu A. |
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Covolan, Luciene [UNIFESP]Almeida, Antonio-Carlos G. deAmorim, Beatriz [UNIFESP]Cavarsan, Clarissa [UNIFESP]Miranda, Maisa FerreiraAarão, Mayra ConsueloMadureira, Ana PaulaRodrigues, Antonio M.Nobrega, Jose N.Mello, Luiz E. [UNIFESP]Hamani, Clement [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Univ Fed Sao Joao del ReiCtr Addict & Mental HlthUniv Toronto2016-01-24T14:37:24Z2016-01-24T14:37:24Z2014-06-03Plos One. San Francisco: Public Library Science, v. 9, n. 6, 6 p., 2014.1932-6203http://repositorio.unifesp.br/handle/11600/37857http://dx.doi.org/10.1371/journal.pone.0097618WOS000336911400016.pdf10.1371/journal.pone.0097618WOS:000336911400016Deep brain stimulation (DBS) has been investigated for the treatment of epilepsy. in rodents, an increase in the latency for the development of seizures and status epilepticus (SE) has been reported in different animal models but the consequences of delivering stimulation to chronic epileptic animals have not been extensively addressed. We study the effects of anterior thalamic nucleus (AN) stimulation at different current intensities in rats rendered epileptic following pilocarpine (Pilo) administration. Four months after Pilo-induced SE, chronic epileptic rats were bilaterally implanted with AN electrodes or had sham-surgery. Stimulation was delivered for 6 h/day, 5 days/week at 130 Hz, 90 mu sec. and either 100 mu A or 500 mu A. the frequency of spontaneous recurrent seizures in animals receiving stimulation was compared to that recorded in the preoperative period and in rats given sham treatment. To investigate the effects of DBS on hippocampal excitability, brain slices from animals receiving AN DBS or sham surgery were studied with electrophysiology. We found that rats treated with AN DBS at 100 mu A had a 52% non-significant reduction in the frequency of seizures as compared to sham-treated controls and 61% less seizures than at baseline. Animals given DBS at 500 mu A had 5.1 times more seizures than controls and a 2.8 fold increase in seizure rate as compared to preoperative values. in non-stimulated controls, the average frequency of seizures before and after surgery remained unaltered. in vitro recordings have shown that slices from animals previously given DBS at 100 mu A had a longer latency for the development of epileptiform activity, shorter and smaller DC shifts, and a smaller spike amplitude compared to non-stimulated controls. in contrast, a higher spike amplitude was recorded in slices from animals given AN DBS at 500 mu A.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Federal de São Paulo, Disciplina Neurofisiol, São Paulo, BrazilUniv Fed Sao Joao del Rei, Lab Neurociencia Expt & Computac, Sao Joao Del Rei, BrazilCtr Addict & Mental Hlth, Behav Neurobiol Lab, Toronto, ON, CanadaUniv Toronto, Toronto Western Hosp, Div Neurosurg, Toronto, ON M5T 2S8, CanadaUniversidade Federal de São Paulo, Disciplina Neurofisiol, São Paulo, BrazilFAPESP: 2011/50680-2FAPESP: 2012/50950-2Web of Science6engPublic Library SciencePlos OneEffects of Anterior Thalamic Nucleus Deep Brain Stimulation in Chronic Epileptic Ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000336911400016.pdfapplication/pdf587685${dspace.ui.url}/bitstream/11600/37857/1/WOS000336911400016.pdf3ed5877d37eae1d30dfa83132f868222MD51open accessTEXTWOS000336911400016.pdf.txtWOS000336911400016.pdf.txtExtracted texttext/plain32385${dspace.ui.url}/bitstream/11600/37857/2/WOS000336911400016.pdf.txt6a293a2630e3daf28a65ab243bc45437MD52open access11600/378572022-11-04 14:22:08.987open accessoai:repositorio.unifesp.br:11600/37857Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-11-04T17:22:08Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Effects of Anterior Thalamic Nucleus Deep Brain Stimulation in Chronic Epileptic Rats |
title |
Effects of Anterior Thalamic Nucleus Deep Brain Stimulation in Chronic Epileptic Rats |
spellingShingle |
Effects of Anterior Thalamic Nucleus Deep Brain Stimulation in Chronic Epileptic Rats Covolan, Luciene [UNIFESP] |
title_short |
Effects of Anterior Thalamic Nucleus Deep Brain Stimulation in Chronic Epileptic Rats |
title_full |
Effects of Anterior Thalamic Nucleus Deep Brain Stimulation in Chronic Epileptic Rats |
title_fullStr |
Effects of Anterior Thalamic Nucleus Deep Brain Stimulation in Chronic Epileptic Rats |
title_full_unstemmed |
Effects of Anterior Thalamic Nucleus Deep Brain Stimulation in Chronic Epileptic Rats |
title_sort |
Effects of Anterior Thalamic Nucleus Deep Brain Stimulation in Chronic Epileptic Rats |
author |
Covolan, Luciene [UNIFESP] |
author_facet |
Covolan, Luciene [UNIFESP] Almeida, Antonio-Carlos G. de Amorim, Beatriz [UNIFESP] Cavarsan, Clarissa [UNIFESP] Miranda, Maisa Ferreira Aarão, Mayra Consuelo Madureira, Ana Paula Rodrigues, Antonio M. Nobrega, Jose N. Mello, Luiz E. [UNIFESP] Hamani, Clement [UNIFESP] |
author_role |
author |
author2 |
Almeida, Antonio-Carlos G. de Amorim, Beatriz [UNIFESP] Cavarsan, Clarissa [UNIFESP] Miranda, Maisa Ferreira Aarão, Mayra Consuelo Madureira, Ana Paula Rodrigues, Antonio M. Nobrega, Jose N. Mello, Luiz E. [UNIFESP] Hamani, Clement [UNIFESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Univ Fed Sao Joao del Rei Ctr Addict & Mental Hlth Univ Toronto |
dc.contributor.author.fl_str_mv |
Covolan, Luciene [UNIFESP] Almeida, Antonio-Carlos G. de Amorim, Beatriz [UNIFESP] Cavarsan, Clarissa [UNIFESP] Miranda, Maisa Ferreira Aarão, Mayra Consuelo Madureira, Ana Paula Rodrigues, Antonio M. Nobrega, Jose N. Mello, Luiz E. [UNIFESP] Hamani, Clement [UNIFESP] |
description |
Deep brain stimulation (DBS) has been investigated for the treatment of epilepsy. in rodents, an increase in the latency for the development of seizures and status epilepticus (SE) has been reported in different animal models but the consequences of delivering stimulation to chronic epileptic animals have not been extensively addressed. We study the effects of anterior thalamic nucleus (AN) stimulation at different current intensities in rats rendered epileptic following pilocarpine (Pilo) administration. Four months after Pilo-induced SE, chronic epileptic rats were bilaterally implanted with AN electrodes or had sham-surgery. Stimulation was delivered for 6 h/day, 5 days/week at 130 Hz, 90 mu sec. and either 100 mu A or 500 mu A. the frequency of spontaneous recurrent seizures in animals receiving stimulation was compared to that recorded in the preoperative period and in rats given sham treatment. To investigate the effects of DBS on hippocampal excitability, brain slices from animals receiving AN DBS or sham surgery were studied with electrophysiology. We found that rats treated with AN DBS at 100 mu A had a 52% non-significant reduction in the frequency of seizures as compared to sham-treated controls and 61% less seizures than at baseline. Animals given DBS at 500 mu A had 5.1 times more seizures than controls and a 2.8 fold increase in seizure rate as compared to preoperative values. in non-stimulated controls, the average frequency of seizures before and after surgery remained unaltered. in vitro recordings have shown that slices from animals previously given DBS at 100 mu A had a longer latency for the development of epileptiform activity, shorter and smaller DC shifts, and a smaller spike amplitude compared to non-stimulated controls. in contrast, a higher spike amplitude was recorded in slices from animals given AN DBS at 500 mu A. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-06-03 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:37:24Z |
dc.date.available.fl_str_mv |
2016-01-24T14:37:24Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Plos One. San Francisco: Public Library Science, v. 9, n. 6, 6 p., 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/37857 http://dx.doi.org/10.1371/journal.pone.0097618 |
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1932-6203 |
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WOS000336911400016.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0097618 |
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WOS:000336911400016 |
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Plos One. San Francisco: Public Library Science, v. 9, n. 6, 6 p., 2014. 1932-6203 WOS000336911400016.pdf 10.1371/journal.pone.0097618 WOS:000336911400016 |
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http://repositorio.unifesp.br/handle/11600/37857 http://dx.doi.org/10.1371/journal.pone.0097618 |
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