Amfenac increases the radiosensitivity of uveal melanoma cell lines
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1038/sj.eye.6703042 http://repositorio.unifesp.br/handle/11600/30610 |
Resumo: | Purpose To evaluate the proliferation rates of five human uveal melanoma (UM) cell lines after treatment with amfenac, a cyclooxygenase (COX)-2 inhibitor, and subsequent radiation exposure.Methods Five human UM cell lines (92.1, SP6.5, MKT-BR, OCM-1, and UW-1) and one human fibroblast cell line (BJ) were incubated with amfenac. Treated and non-treated cell lines were then exposed to various doses of gamma radiation: 0, 2, 4, 6, and 8 Gy. Sulphorhodamine-B assay was used to assess proliferation rates 48 h post-radiation.Results Treatment of UM cell lines with amfenac prior to radiation led to a marked reduction in proliferation rates. This difference was statistically significant in all cell lines at every radiation dose (P < 0.005), with the exception of 92.1 at 2 Gy (P=0.157). Fibroblasts treated with amfenac showed significantly higher proliferation rates after 2 and 8 Gy, with no significant differences at 0, 4, and 6 Gy.Conclusions the radiosensitivity of UM cell lines was increased by the administration of amfenac, the active metabolite of nepafenac. There appears to be a radioprotective effect of amfenac on human fibroblasts. the topical administration of nepafenac may decrease tumour recurrence and radiation-induced complications while broadening the indications for radiotherapy by treating larger tumours. |
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Amfenac increases the radiosensitivity of uveal melanoma cell linesuveal melanomaradiationCOX-2amfenaccell linesPurpose To evaluate the proliferation rates of five human uveal melanoma (UM) cell lines after treatment with amfenac, a cyclooxygenase (COX)-2 inhibitor, and subsequent radiation exposure.Methods Five human UM cell lines (92.1, SP6.5, MKT-BR, OCM-1, and UW-1) and one human fibroblast cell line (BJ) were incubated with amfenac. Treated and non-treated cell lines were then exposed to various doses of gamma radiation: 0, 2, 4, 6, and 8 Gy. Sulphorhodamine-B assay was used to assess proliferation rates 48 h post-radiation.Results Treatment of UM cell lines with amfenac prior to radiation led to a marked reduction in proliferation rates. This difference was statistically significant in all cell lines at every radiation dose (P < 0.005), with the exception of 92.1 at 2 Gy (P=0.157). Fibroblasts treated with amfenac showed significantly higher proliferation rates after 2 and 8 Gy, with no significant differences at 0, 4, and 6 Gy.Conclusions the radiosensitivity of UM cell lines was increased by the administration of amfenac, the active metabolite of nepafenac. There appears to be a radioprotective effect of amfenac on human fibroblasts. the topical administration of nepafenac may decrease tumour recurrence and radiation-induced complications while broadening the indications for radiotherapy by treating larger tumours.McGill Univ, Dept Ophthalmol & Pathol, Ctr Hlth, Montreal, PQ H3A 2B4, CanadaHenry C Witelson Ocular Pathol Lab, Montreal, PQ H3A 2B4, CanadaUniversidade Federal de São Paulo, UNIFESP EPM, Dept Ophthalmol, São Paulo, BrazilUniversidade Federal de São Paulo, UNIFESP EPM, Dept Ophthalmol, São Paulo, BrazilWeb of ScienceNature Publishing GroupMcGill UnivHenry C Witelson Ocular Pathol LabUniversidade Federal de São Paulo (UNIFESP)Fernandes, B. F.Marshall, J-CDi Cesare, S.Logan, P.Maloney, S.Burnier Júnior, Miguel Noel Nascente [UNIFESP]2016-01-24T13:49:46Z2016-01-24T13:49:46Z2008-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion701-706http://dx.doi.org/10.1038/sj.eye.6703042Eye. London: Nature Publishing Group, v. 22, n. 5, p. 701-706, 2008.10.1038/sj.eye.67030420950-222Xhttp://repositorio.unifesp.br/handle/11600/30610WOS:000255897300018engEyeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2022-02-07T21:51:36Zoai:repositorio.unifesp.br/:11600/30610Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652022-02-07T21:51:36Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Amfenac increases the radiosensitivity of uveal melanoma cell lines |
title |
Amfenac increases the radiosensitivity of uveal melanoma cell lines |
spellingShingle |
Amfenac increases the radiosensitivity of uveal melanoma cell lines Fernandes, B. F. uveal melanoma radiation COX-2 amfenac cell lines |
title_short |
Amfenac increases the radiosensitivity of uveal melanoma cell lines |
title_full |
Amfenac increases the radiosensitivity of uveal melanoma cell lines |
title_fullStr |
Amfenac increases the radiosensitivity of uveal melanoma cell lines |
title_full_unstemmed |
Amfenac increases the radiosensitivity of uveal melanoma cell lines |
title_sort |
Amfenac increases the radiosensitivity of uveal melanoma cell lines |
author |
Fernandes, B. F. |
author_facet |
Fernandes, B. F. Marshall, J-C Di Cesare, S. Logan, P. Maloney, S. Burnier Júnior, Miguel Noel Nascente [UNIFESP] |
author_role |
author |
author2 |
Marshall, J-C Di Cesare, S. Logan, P. Maloney, S. Burnier Júnior, Miguel Noel Nascente [UNIFESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
McGill Univ Henry C Witelson Ocular Pathol Lab Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Fernandes, B. F. Marshall, J-C Di Cesare, S. Logan, P. Maloney, S. Burnier Júnior, Miguel Noel Nascente [UNIFESP] |
dc.subject.por.fl_str_mv |
uveal melanoma radiation COX-2 amfenac cell lines |
topic |
uveal melanoma radiation COX-2 amfenac cell lines |
description |
Purpose To evaluate the proliferation rates of five human uveal melanoma (UM) cell lines after treatment with amfenac, a cyclooxygenase (COX)-2 inhibitor, and subsequent radiation exposure.Methods Five human UM cell lines (92.1, SP6.5, MKT-BR, OCM-1, and UW-1) and one human fibroblast cell line (BJ) were incubated with amfenac. Treated and non-treated cell lines were then exposed to various doses of gamma radiation: 0, 2, 4, 6, and 8 Gy. Sulphorhodamine-B assay was used to assess proliferation rates 48 h post-radiation.Results Treatment of UM cell lines with amfenac prior to radiation led to a marked reduction in proliferation rates. This difference was statistically significant in all cell lines at every radiation dose (P < 0.005), with the exception of 92.1 at 2 Gy (P=0.157). Fibroblasts treated with amfenac showed significantly higher proliferation rates after 2 and 8 Gy, with no significant differences at 0, 4, and 6 Gy.Conclusions the radiosensitivity of UM cell lines was increased by the administration of amfenac, the active metabolite of nepafenac. There appears to be a radioprotective effect of amfenac on human fibroblasts. the topical administration of nepafenac may decrease tumour recurrence and radiation-induced complications while broadening the indications for radiotherapy by treating larger tumours. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-05-01 2016-01-24T13:49:46Z 2016-01-24T13:49:46Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/sj.eye.6703042 Eye. London: Nature Publishing Group, v. 22, n. 5, p. 701-706, 2008. 10.1038/sj.eye.6703042 0950-222X http://repositorio.unifesp.br/handle/11600/30610 WOS:000255897300018 |
url |
http://dx.doi.org/10.1038/sj.eye.6703042 http://repositorio.unifesp.br/handle/11600/30610 |
identifier_str_mv |
Eye. London: Nature Publishing Group, v. 22, n. 5, p. 701-706, 2008. 10.1038/sj.eye.6703042 0950-222X WOS:000255897300018 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Eye |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
701-706 |
dc.publisher.none.fl_str_mv |
Nature Publishing Group |
publisher.none.fl_str_mv |
Nature Publishing Group |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1814268315110998016 |