Amfenac increases the radiosensitivity of uveal melanoma cell lines

Detalhes bibliográficos
Autor(a) principal: Fernandes, B. F.
Data de Publicação: 2008
Outros Autores: Marshall, J-C, Di Cesare, S., Logan, P., Maloney, S., Burnier Júnior, Miguel Noel Nascente [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1038/sj.eye.6703042
http://repositorio.unifesp.br/handle/11600/30610
Resumo: Purpose To evaluate the proliferation rates of five human uveal melanoma (UM) cell lines after treatment with amfenac, a cyclooxygenase (COX)-2 inhibitor, and subsequent radiation exposure.Methods Five human UM cell lines (92.1, SP6.5, MKT-BR, OCM-1, and UW-1) and one human fibroblast cell line (BJ) were incubated with amfenac. Treated and non-treated cell lines were then exposed to various doses of gamma radiation: 0, 2, 4, 6, and 8 Gy. Sulphorhodamine-B assay was used to assess proliferation rates 48 h post-radiation.Results Treatment of UM cell lines with amfenac prior to radiation led to a marked reduction in proliferation rates. This difference was statistically significant in all cell lines at every radiation dose (P < 0.005), with the exception of 92.1 at 2 Gy (P=0.157). Fibroblasts treated with amfenac showed significantly higher proliferation rates after 2 and 8 Gy, with no significant differences at 0, 4, and 6 Gy.Conclusions the radiosensitivity of UM cell lines was increased by the administration of amfenac, the active metabolite of nepafenac. There appears to be a radioprotective effect of amfenac on human fibroblasts. the topical administration of nepafenac may decrease tumour recurrence and radiation-induced complications while broadening the indications for radiotherapy by treating larger tumours.
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spelling Amfenac increases the radiosensitivity of uveal melanoma cell linesuveal melanomaradiationCOX-2amfenaccell linesPurpose To evaluate the proliferation rates of five human uveal melanoma (UM) cell lines after treatment with amfenac, a cyclooxygenase (COX)-2 inhibitor, and subsequent radiation exposure.Methods Five human UM cell lines (92.1, SP6.5, MKT-BR, OCM-1, and UW-1) and one human fibroblast cell line (BJ) were incubated with amfenac. Treated and non-treated cell lines were then exposed to various doses of gamma radiation: 0, 2, 4, 6, and 8 Gy. Sulphorhodamine-B assay was used to assess proliferation rates 48 h post-radiation.Results Treatment of UM cell lines with amfenac prior to radiation led to a marked reduction in proliferation rates. This difference was statistically significant in all cell lines at every radiation dose (P < 0.005), with the exception of 92.1 at 2 Gy (P=0.157). Fibroblasts treated with amfenac showed significantly higher proliferation rates after 2 and 8 Gy, with no significant differences at 0, 4, and 6 Gy.Conclusions the radiosensitivity of UM cell lines was increased by the administration of amfenac, the active metabolite of nepafenac. There appears to be a radioprotective effect of amfenac on human fibroblasts. the topical administration of nepafenac may decrease tumour recurrence and radiation-induced complications while broadening the indications for radiotherapy by treating larger tumours.McGill Univ, Dept Ophthalmol & Pathol, Ctr Hlth, Montreal, PQ H3A 2B4, CanadaHenry C Witelson Ocular Pathol Lab, Montreal, PQ H3A 2B4, CanadaUniversidade Federal de São Paulo, UNIFESP EPM, Dept Ophthalmol, São Paulo, BrazilUniversidade Federal de São Paulo, UNIFESP EPM, Dept Ophthalmol, São Paulo, BrazilWeb of ScienceNature Publishing GroupMcGill UnivHenry C Witelson Ocular Pathol LabUniversidade Federal de São Paulo (UNIFESP)Fernandes, B. F.Marshall, J-CDi Cesare, S.Logan, P.Maloney, S.Burnier Júnior, Miguel Noel Nascente [UNIFESP]2016-01-24T13:49:46Z2016-01-24T13:49:46Z2008-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion701-706http://dx.doi.org/10.1038/sj.eye.6703042Eye. London: Nature Publishing Group, v. 22, n. 5, p. 701-706, 2008.10.1038/sj.eye.67030420950-222Xhttp://repositorio.unifesp.br/handle/11600/30610WOS:000255897300018engEyeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2022-02-07T21:51:36Zoai:repositorio.unifesp.br/:11600/30610Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652022-02-07T21:51:36Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Amfenac increases the radiosensitivity of uveal melanoma cell lines
title Amfenac increases the radiosensitivity of uveal melanoma cell lines
spellingShingle Amfenac increases the radiosensitivity of uveal melanoma cell lines
Fernandes, B. F.
uveal melanoma
radiation
COX-2
amfenac
cell lines
title_short Amfenac increases the radiosensitivity of uveal melanoma cell lines
title_full Amfenac increases the radiosensitivity of uveal melanoma cell lines
title_fullStr Amfenac increases the radiosensitivity of uveal melanoma cell lines
title_full_unstemmed Amfenac increases the radiosensitivity of uveal melanoma cell lines
title_sort Amfenac increases the radiosensitivity of uveal melanoma cell lines
author Fernandes, B. F.
author_facet Fernandes, B. F.
Marshall, J-C
Di Cesare, S.
Logan, P.
Maloney, S.
Burnier Júnior, Miguel Noel Nascente [UNIFESP]
author_role author
author2 Marshall, J-C
Di Cesare, S.
Logan, P.
Maloney, S.
Burnier Júnior, Miguel Noel Nascente [UNIFESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv McGill Univ
Henry C Witelson Ocular Pathol Lab
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Fernandes, B. F.
Marshall, J-C
Di Cesare, S.
Logan, P.
Maloney, S.
Burnier Júnior, Miguel Noel Nascente [UNIFESP]
dc.subject.por.fl_str_mv uveal melanoma
radiation
COX-2
amfenac
cell lines
topic uveal melanoma
radiation
COX-2
amfenac
cell lines
description Purpose To evaluate the proliferation rates of five human uveal melanoma (UM) cell lines after treatment with amfenac, a cyclooxygenase (COX)-2 inhibitor, and subsequent radiation exposure.Methods Five human UM cell lines (92.1, SP6.5, MKT-BR, OCM-1, and UW-1) and one human fibroblast cell line (BJ) were incubated with amfenac. Treated and non-treated cell lines were then exposed to various doses of gamma radiation: 0, 2, 4, 6, and 8 Gy. Sulphorhodamine-B assay was used to assess proliferation rates 48 h post-radiation.Results Treatment of UM cell lines with amfenac prior to radiation led to a marked reduction in proliferation rates. This difference was statistically significant in all cell lines at every radiation dose (P < 0.005), with the exception of 92.1 at 2 Gy (P=0.157). Fibroblasts treated with amfenac showed significantly higher proliferation rates after 2 and 8 Gy, with no significant differences at 0, 4, and 6 Gy.Conclusions the radiosensitivity of UM cell lines was increased by the administration of amfenac, the active metabolite of nepafenac. There appears to be a radioprotective effect of amfenac on human fibroblasts. the topical administration of nepafenac may decrease tumour recurrence and radiation-induced complications while broadening the indications for radiotherapy by treating larger tumours.
publishDate 2008
dc.date.none.fl_str_mv 2008-05-01
2016-01-24T13:49:46Z
2016-01-24T13:49:46Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1038/sj.eye.6703042
Eye. London: Nature Publishing Group, v. 22, n. 5, p. 701-706, 2008.
10.1038/sj.eye.6703042
0950-222X
http://repositorio.unifesp.br/handle/11600/30610
WOS:000255897300018
url http://dx.doi.org/10.1038/sj.eye.6703042
http://repositorio.unifesp.br/handle/11600/30610
identifier_str_mv Eye. London: Nature Publishing Group, v. 22, n. 5, p. 701-706, 2008.
10.1038/sj.eye.6703042
0950-222X
WOS:000255897300018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Eye
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 701-706
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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