Transcriptome Profile of Trichoderma harzianum IOC-3844 Induced by Sugarcane Bagasse
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/37443 http://dx.doi.org/10.1371/journal.pone.0088689 |
Resumo: | Profiling the transcriptome that underlies biomass degradation by the fungus Trichoderma harzianum allows the identification of gene sequences with potential application in enzymatic hydrolysis processing. in the present study, the transcriptome of T. harzianum IOC-3844 was analyzed using RNA-seq technology. the sequencing generated 14.7 Gbp for downstream analyses. de novo assembly resulted in 32,396 contigs, which were submitted for identification and classified according to their identities. This analysis allowed us to define a principal set of T. harzianum genes that are involved in the degradation of cellulose and hemicellulose and the accessory genes that are involved in the depolymerization of biomass. An additional analysis of expression levels identified a set of carbohydrate-active enzymes that are upregulated under different conditions. the present study provides valuable information for future studies on biomass degradation and contributes to a better understanding of the role of the genes that are involved in this process. |
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Crivelente Horta, Maria AugustaVicentini, RenatoDelabona, Priscila da SilvaLaborda, PriandaCrucello, AlineFreitas, SindeliaKuroshu, Reginaldo Massanobu [UNIFESP]Polikarpov, IgorCruz Pradella, Jose Geraldo daSouza, Anete PereiraUniversidade Estadual de Campinas (UNICAMP)Brazilian Ctr Res Energy & Mat CNPEMUniversidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)2016-01-24T14:35:19Z2016-01-24T14:35:19Z2014-02-18Plos One. San Francisco: Public Library Science, v. 9, n. 2, 17 p., 2014.1932-6203http://repositorio.unifesp.br/handle/11600/37443http://dx.doi.org/10.1371/journal.pone.0088689WOS000331706700059.pdf10.1371/journal.pone.0088689WOS:000331706700059Profiling the transcriptome that underlies biomass degradation by the fungus Trichoderma harzianum allows the identification of gene sequences with potential application in enzymatic hydrolysis processing. in the present study, the transcriptome of T. harzianum IOC-3844 was analyzed using RNA-seq technology. the sequencing generated 14.7 Gbp for downstream analyses. de novo assembly resulted in 32,396 contigs, which were submitted for identification and classified according to their identities. This analysis allowed us to define a principal set of T. harzianum genes that are involved in the degradation of cellulose and hemicellulose and the accessory genes that are involved in the depolymerization of biomass. An additional analysis of expression levels identified a set of carbohydrate-active enzymes that are upregulated under different conditions. the present study provides valuable information for future studies on biomass degradation and contributes to a better understanding of the role of the genes that are involved in this process.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Campinas UNICAMP, CBMEG, Campinas, SP, BrazilBrazilian Ctr Res Energy & Mat CNPEM, Brazilian Bioethanol Sci & Technol Lab CTBE, Campinas, SP, BrazilUniv São Paulo, Phys Inst Sao Carlos, Sao Carlos, SP, BrazilFed Univ São Paulo UNIFESP, Inst Sci & Technol, Sao Jose Dos Campos, SP, BrazilUniv Campinas UNICAMP, Dept Plant Biol, Inst Biol, Campinas, SP, BrazilFed Univ São Paulo UNIFESP, Inst Sci & Technol, Sao Jose Dos Campos, SP, BrazilWeb of Science17engPublic Library SciencePlos OneTranscriptome Profile of Trichoderma harzianum IOC-3844 Induced by Sugarcane Bagasseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000331706700059.pdfapplication/pdf1563400${dspace.ui.url}/bitstream/11600/37443/1/WOS000331706700059.pdfb2ec3baeb626bd3b2a9dd35c7cc8c610MD51open accessTEXTWOS000331706700059.pdf.txtWOS000331706700059.pdf.txtExtracted texttext/plain66231${dspace.ui.url}/bitstream/11600/37443/9/WOS000331706700059.pdf.txt9136255b45e6e1075591ecec4c46923aMD59open accessTHUMBNAILWOS000331706700059.pdf.jpgWOS000331706700059.pdf.jpgIM Thumbnailimage/jpeg7857${dspace.ui.url}/bitstream/11600/37443/11/WOS000331706700059.pdf.jpge5bf2e86f8d8ddcb90d0ccd3cfd0a90fMD511open access11600/374432023-06-05 19:09:23.743open accessoai:repositorio.unifesp.br:11600/37443Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:09:23Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Transcriptome Profile of Trichoderma harzianum IOC-3844 Induced by Sugarcane Bagasse |
title |
Transcriptome Profile of Trichoderma harzianum IOC-3844 Induced by Sugarcane Bagasse |
spellingShingle |
Transcriptome Profile of Trichoderma harzianum IOC-3844 Induced by Sugarcane Bagasse Crivelente Horta, Maria Augusta |
title_short |
Transcriptome Profile of Trichoderma harzianum IOC-3844 Induced by Sugarcane Bagasse |
title_full |
Transcriptome Profile of Trichoderma harzianum IOC-3844 Induced by Sugarcane Bagasse |
title_fullStr |
Transcriptome Profile of Trichoderma harzianum IOC-3844 Induced by Sugarcane Bagasse |
title_full_unstemmed |
Transcriptome Profile of Trichoderma harzianum IOC-3844 Induced by Sugarcane Bagasse |
title_sort |
Transcriptome Profile of Trichoderma harzianum IOC-3844 Induced by Sugarcane Bagasse |
author |
Crivelente Horta, Maria Augusta |
author_facet |
Crivelente Horta, Maria Augusta Vicentini, Renato Delabona, Priscila da Silva Laborda, Prianda Crucello, Aline Freitas, Sindelia Kuroshu, Reginaldo Massanobu [UNIFESP] Polikarpov, Igor Cruz Pradella, Jose Geraldo da Souza, Anete Pereira |
author_role |
author |
author2 |
Vicentini, Renato Delabona, Priscila da Silva Laborda, Prianda Crucello, Aline Freitas, Sindelia Kuroshu, Reginaldo Massanobu [UNIFESP] Polikarpov, Igor Cruz Pradella, Jose Geraldo da Souza, Anete Pereira |
author2_role |
author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Brazilian Ctr Res Energy & Mat CNPEM Universidade de São Paulo (USP) Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Crivelente Horta, Maria Augusta Vicentini, Renato Delabona, Priscila da Silva Laborda, Prianda Crucello, Aline Freitas, Sindelia Kuroshu, Reginaldo Massanobu [UNIFESP] Polikarpov, Igor Cruz Pradella, Jose Geraldo da Souza, Anete Pereira |
description |
Profiling the transcriptome that underlies biomass degradation by the fungus Trichoderma harzianum allows the identification of gene sequences with potential application in enzymatic hydrolysis processing. in the present study, the transcriptome of T. harzianum IOC-3844 was analyzed using RNA-seq technology. the sequencing generated 14.7 Gbp for downstream analyses. de novo assembly resulted in 32,396 contigs, which were submitted for identification and classified according to their identities. This analysis allowed us to define a principal set of T. harzianum genes that are involved in the degradation of cellulose and hemicellulose and the accessory genes that are involved in the depolymerization of biomass. An additional analysis of expression levels identified a set of carbohydrate-active enzymes that are upregulated under different conditions. the present study provides valuable information for future studies on biomass degradation and contributes to a better understanding of the role of the genes that are involved in this process. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-02-18 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:35:19Z |
dc.date.available.fl_str_mv |
2016-01-24T14:35:19Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Plos One. San Francisco: Public Library Science, v. 9, n. 2, 17 p., 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/37443 http://dx.doi.org/10.1371/journal.pone.0088689 |
dc.identifier.issn.none.fl_str_mv |
1932-6203 |
dc.identifier.file.none.fl_str_mv |
WOS000331706700059.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0088689 |
dc.identifier.wos.none.fl_str_mv |
WOS:000331706700059 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 9, n. 2, 17 p., 2014. 1932-6203 WOS000331706700059.pdf 10.1371/journal.pone.0088689 WOS:000331706700059 |
url |
http://repositorio.unifesp.br/handle/11600/37443 http://dx.doi.org/10.1371/journal.pone.0088689 |
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eng |
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17 |
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Public Library Science |
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Public Library Science |
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