Impaired production of interferon-gamma and tumor necrosis factor-alpha but not of interleukin 10 in whole blood of patients with sepsis

Detalhes bibliográficos
Autor(a) principal: Rigato, Otelo [UNIFESP]
Data de Publicação: 2003
Outros Autores: Salomão, Reinaldo [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://repositorio.unifesp.br/handle/11600/27123
http://dx.doi.org/10.1097/01.SHK.0000054740.68620.c4
Resumo: It has been demonstrated that lipopolysaccharide (LPS)-induced cytokine response in patients with sepsis differ from the normal host, yet this has not been controlled for the presence of underlying disease. We studied the ability of LPS and killed gram-negative bacteria (GNB) to induce tumor necrosis factor (TNF)-alpha and interleukin (IL) 10, and of phytohemagglutinin (PHA) to induce interferon (IFN)-gamma, in whole blood from patients with sepsis (SP, n = 20), patients with matched underlying disease and without sepsis (control patients, n = 20), and healthy volunteers (HV, n = 20). LPS-induced TNF-alpha production was lower in SP (median = 638 pg/mL) compared with control patients (4060 pg/mL; P = 0.003), and control patients production was lower compared with HV (5329 pg/mL; P < 0.001). Pseudomonas aeruginosa-induced TNF-&alpha; production was lower in SP (1443 pg/mL) than in control patients (7319 pg/mL; P < 0.05), and was not different between control patients and HV (6612 pg/mL; P = 0.6). IFNgamma production was lower in SP (948 pg/mL) compared with control patients (5516 pg/mL; P < 0.001), and the control patients production was lowercompared with HV (11,282 pg/mL; P < 0.001). IL-10 production was not different among the three groups. Down-regulation of TNF-alpha production in patients with sepsis, although not restricted to them, was more pronounced with LPS than with GNB. Although the presence of underlying disease may be involved in the regulatory mechanisms of host response, the use of controls with matched underlying diseases provides strong evidence for the septic condition in the down-regulation of inflammatory response in patients with sepsis.
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spelling Rigato, Otelo [UNIFESP]Salomão, Reinaldo [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2016-01-24T12:33:42Z2016-01-24T12:33:42Z2003-02-01Shock. Philadelphia: Lippincott Williams & Wilkins, v. 19, n. 2, p. 113-116, 2003.1073-2322http://repositorio.unifesp.br/handle/11600/27123http://dx.doi.org/10.1097/01.SHK.0000054740.68620.c410.1097/01.SHK.0000054740.68620.c4WOS:000180668600004It has been demonstrated that lipopolysaccharide (LPS)-induced cytokine response in patients with sepsis differ from the normal host, yet this has not been controlled for the presence of underlying disease. We studied the ability of LPS and killed gram-negative bacteria (GNB) to induce tumor necrosis factor (TNF)-alpha and interleukin (IL) 10, and of phytohemagglutinin (PHA) to induce interferon (IFN)-gamma, in whole blood from patients with sepsis (SP, n = 20), patients with matched underlying disease and without sepsis (control patients, n = 20), and healthy volunteers (HV, n = 20). LPS-induced TNF-alpha production was lower in SP (median = 638 pg/mL) compared with control patients (4060 pg/mL; P = 0.003), and control patients production was lower compared with HV (5329 pg/mL; P < 0.001). Pseudomonas aeruginosa-induced TNF-&alpha; production was lower in SP (1443 pg/mL) than in control patients (7319 pg/mL; P < 0.05), and was not different between control patients and HV (6612 pg/mL; P = 0.6). IFNgamma production was lower in SP (948 pg/mL) compared with control patients (5516 pg/mL; P < 0.001), and the control patients production was lowercompared with HV (11,282 pg/mL; P < 0.001). IL-10 production was not different among the three groups. Down-regulation of TNF-alpha production in patients with sepsis, although not restricted to them, was more pronounced with LPS than with GNB. Although the presence of underlying disease may be involved in the regulatory mechanisms of host response, the use of controls with matched underlying diseases provides strong evidence for the septic condition in the down-regulation of inflammatory response in patients with sepsis.Universidade Federal de São Paulo, Immunol Lab, Div Infect Dis, Escola Paulista Med, BR-04039032 São Paulo, BrazilUniversidade Federal de São Paulo, Immunol Lab, Div Infect Dis, Escola Paulista Med, BR-04039032 São Paulo, BrazilWeb of Science113-116engLippincott Williams & WilkinsShocklipopolysaccharidewhole bloodcytokinessepsisunderlying diseaseImpaired production of interferon-gamma and tumor necrosis factor-alpha but not of interleukin 10 in whole blood of patients with sepsisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/271232022-11-03 15:00:30.447metadata only accessoai:repositorio.unifesp.br:11600/27123Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:28:04.549888Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.en.fl_str_mv Impaired production of interferon-gamma and tumor necrosis factor-alpha but not of interleukin 10 in whole blood of patients with sepsis
title Impaired production of interferon-gamma and tumor necrosis factor-alpha but not of interleukin 10 in whole blood of patients with sepsis
spellingShingle Impaired production of interferon-gamma and tumor necrosis factor-alpha but not of interleukin 10 in whole blood of patients with sepsis
Rigato, Otelo [UNIFESP]
lipopolysaccharide
whole blood
cytokines
sepsis
underlying disease
title_short Impaired production of interferon-gamma and tumor necrosis factor-alpha but not of interleukin 10 in whole blood of patients with sepsis
title_full Impaired production of interferon-gamma and tumor necrosis factor-alpha but not of interleukin 10 in whole blood of patients with sepsis
title_fullStr Impaired production of interferon-gamma and tumor necrosis factor-alpha but not of interleukin 10 in whole blood of patients with sepsis
title_full_unstemmed Impaired production of interferon-gamma and tumor necrosis factor-alpha but not of interleukin 10 in whole blood of patients with sepsis
title_sort Impaired production of interferon-gamma and tumor necrosis factor-alpha but not of interleukin 10 in whole blood of patients with sepsis
author Rigato, Otelo [UNIFESP]
author_facet Rigato, Otelo [UNIFESP]
Salomão, Reinaldo [UNIFESP]
author_role author
author2 Salomão, Reinaldo [UNIFESP]
author2_role author
dc.contributor.institution.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Rigato, Otelo [UNIFESP]
Salomão, Reinaldo [UNIFESP]
dc.subject.eng.fl_str_mv lipopolysaccharide
whole blood
cytokines
sepsis
underlying disease
topic lipopolysaccharide
whole blood
cytokines
sepsis
underlying disease
description It has been demonstrated that lipopolysaccharide (LPS)-induced cytokine response in patients with sepsis differ from the normal host, yet this has not been controlled for the presence of underlying disease. We studied the ability of LPS and killed gram-negative bacteria (GNB) to induce tumor necrosis factor (TNF)-alpha and interleukin (IL) 10, and of phytohemagglutinin (PHA) to induce interferon (IFN)-gamma, in whole blood from patients with sepsis (SP, n = 20), patients with matched underlying disease and without sepsis (control patients, n = 20), and healthy volunteers (HV, n = 20). LPS-induced TNF-alpha production was lower in SP (median = 638 pg/mL) compared with control patients (4060 pg/mL; P = 0.003), and control patients production was lower compared with HV (5329 pg/mL; P < 0.001). Pseudomonas aeruginosa-induced TNF-&alpha; production was lower in SP (1443 pg/mL) than in control patients (7319 pg/mL; P < 0.05), and was not different between control patients and HV (6612 pg/mL; P = 0.6). IFNgamma production was lower in SP (948 pg/mL) compared with control patients (5516 pg/mL; P < 0.001), and the control patients production was lowercompared with HV (11,282 pg/mL; P < 0.001). IL-10 production was not different among the three groups. Down-regulation of TNF-alpha production in patients with sepsis, although not restricted to them, was more pronounced with LPS than with GNB. Although the presence of underlying disease may be involved in the regulatory mechanisms of host response, the use of controls with matched underlying diseases provides strong evidence for the septic condition in the down-regulation of inflammatory response in patients with sepsis.
publishDate 2003
dc.date.issued.fl_str_mv 2003-02-01
dc.date.accessioned.fl_str_mv 2016-01-24T12:33:42Z
dc.date.available.fl_str_mv 2016-01-24T12:33:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv Shock. Philadelphia: Lippincott Williams & Wilkins, v. 19, n. 2, p. 113-116, 2003.
dc.identifier.uri.fl_str_mv http://repositorio.unifesp.br/handle/11600/27123
http://dx.doi.org/10.1097/01.SHK.0000054740.68620.c4
dc.identifier.issn.none.fl_str_mv 1073-2322
dc.identifier.doi.none.fl_str_mv 10.1097/01.SHK.0000054740.68620.c4
dc.identifier.wos.none.fl_str_mv WOS:000180668600004
identifier_str_mv Shock. Philadelphia: Lippincott Williams & Wilkins, v. 19, n. 2, p. 113-116, 2003.
1073-2322
10.1097/01.SHK.0000054740.68620.c4
WOS:000180668600004
url http://repositorio.unifesp.br/handle/11600/27123
http://dx.doi.org/10.1097/01.SHK.0000054740.68620.c4
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv Shock
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 113-116
dc.publisher.none.fl_str_mv Lippincott Williams & Wilkins
publisher.none.fl_str_mv Lippincott Williams & Wilkins
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv
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