Oleic, Linoleic and Linolenic Acids Increase ROS Production by Fibroblasts via NADPH Oxidase Activation

• Autor(a) principal: Hatanaka, Elaine
• Data de Publicação: 2013
• Outros Autores: Dermargos, Alexandre, Hirata, Aparecida Emiko [UNIFESP], Ramirez Vinolo, Marco Aurelio, Carpinelli, Angelo Rafael, Newsholme, Philip, Armelin, Hugo Aguirre, Curi, Rui
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.1371/journal.pone.0058626; http://repositorio.unifesp.br/handle/11600/36199

The effect of oleic, linoleic and gamma-linolenic acids on ROS production by 3T3 Swiss and Rat 1 fibroblasts was investigated. Using lucigenin-amplified chemiluminescence, a dose-dependent increase in extracellular superoxide levels was observed during the treatment of fibroblasts with oleic, linoleic and gamma-linolenic acids. ROS production was dependent on the addition of beta-NADH or NADPH to the medium. Diphenyleneiodonium inhibited the effect of oleic, linoleic and gamma-linolenic acids on fibroblast superoxide release by 79%, 92% and 82%, respectively. Increased levels of p47(phox) phosphorylation due to fatty acid treatment were detected by Western blotting analyses of fibroblast proteins. Increased p47(phox) mRNA expression was observed using real-time PCR. the rank order for the fatty acid stimulation of the fibroblast oxidative burst was as follows: gamma-linolenic > linoleic > oleic. in conclusion, oleic, linoleic and gamma-linolenic acids stimulated ROS production via activation of the NADPH oxidase enzyme complex in fibroblasts.