A non-hemorrhagic hybrid heparin/heparan sulfate with anticoagulant potential
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1016/j.carbpol.2013.08.063 http://repositorio.unifesp.br/handle/11600/37305 |
Resumo: | The structural characterization and the anticoagulant potential of a novel heparin/heparan sulfate-like compound from the heads of Litopenaeus vannamei shrimp are described. While it is distinct from either heparin or heparan sulfate, enzymatic depolymerization and nuclear magnetic resonance spectroscopy analyses revealed that this molecule does share some structural features with heparin, such as the high degree of N- and 6-0-sulfation and minor N-acetylation, and with heparan sulfate, in the glucuronic acid content. Its ability to stabilize human antithrombin explains its significant anticoagulant activity in aPTT and Factor-Xa inhibition assays. Interestingly, in contrast to mammalian heparin, the shrimp compound displayed negligible hemorrhagic effect. Together, these findings have particular interest since they reveal a novel molecule with significant anti-Xa activity coupled with low bleeding effects which make the shrimp heparin/HS-like compound a potential alternative for mammalian heparin. (C) 2013 Elsevier B.V. All rights reserved. |
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A non-hemorrhagic hybrid heparin/heparan sulfate with anticoagulant potentialHeparinHeparan sulfateHemorrhagic activityAntithrombinAnticoagulantThermostabilizationThe structural characterization and the anticoagulant potential of a novel heparin/heparan sulfate-like compound from the heads of Litopenaeus vannamei shrimp are described. While it is distinct from either heparin or heparan sulfate, enzymatic depolymerization and nuclear magnetic resonance spectroscopy analyses revealed that this molecule does share some structural features with heparin, such as the high degree of N- and 6-0-sulfation and minor N-acetylation, and with heparan sulfate, in the glucuronic acid content. Its ability to stabilize human antithrombin explains its significant anticoagulant activity in aPTT and Factor-Xa inhibition assays. Interestingly, in contrast to mammalian heparin, the shrimp compound displayed negligible hemorrhagic effect. Together, these findings have particular interest since they reveal a novel molecule with significant anti-Xa activity coupled with low bleeding effects which make the shrimp heparin/HS-like compound a potential alternative for mammalian heparin. (C) 2013 Elsevier B.V. All rights reserved.Univ Fed Rio Grande do Norte, Dept Bioquim, BR-59072970 Natal, RN, BrazilUniv Fed Rio Grande do Norte, Fac Ciencias Saude Trairi, Santa Cruz, RN, BrazilUniv Liverpool, Dept Struct & Chem Biol, Liverpool L69 7ZB, Merseyside, EnglandDiamond Light Source Ltd, Didcot OX11 0DE, Oxon, EnglandUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, BrazilWeb of SciencePPG.BIOQ (Programa de Pos-graduagao em Bioquimica/UFRN)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)LaisElsevier B.V.Univ Fed Rio Grande do NorteUniv LiverpoolDiamond Light Source LtdUniversidade Federal de São Paulo (UNIFESP)Brito, Adriana S.Cavalcante, Romulo S.Palhares, Lais C. G. F.Hughes, Ashley J.Andrade, Giulianna P. V.Yates, Edwin Alexander [UNIFESP]Nader, Helena Bonciani [UNIFESP]Lima, Marcelo Andrade de [UNIFESP]Chavante, Suely F.2016-01-24T14:35:08Z2016-01-24T14:35:08Z2014-01-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion372-378application/pdfhttp://dx.doi.org/10.1016/j.carbpol.2013.08.063Carbohydrate Polymers. Oxford: Elsevier B.V., v. 99, p. 372-378, 2014.10.1016/j.carbpol.2013.08.063WOS000329256600048.pdf0144-8617http://repositorio.unifesp.br/handle/11600/37305WOS:000329256600048engCarbohydrate Polymersinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-08T05:09:46Zoai:repositorio.unifesp.br/:11600/37305Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-08T05:09:46Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
A non-hemorrhagic hybrid heparin/heparan sulfate with anticoagulant potential |
title |
A non-hemorrhagic hybrid heparin/heparan sulfate with anticoagulant potential |
spellingShingle |
A non-hemorrhagic hybrid heparin/heparan sulfate with anticoagulant potential Brito, Adriana S. Heparin Heparan sulfate Hemorrhagic activity Antithrombin Anticoagulant Thermostabilization |
title_short |
A non-hemorrhagic hybrid heparin/heparan sulfate with anticoagulant potential |
title_full |
A non-hemorrhagic hybrid heparin/heparan sulfate with anticoagulant potential |
title_fullStr |
A non-hemorrhagic hybrid heparin/heparan sulfate with anticoagulant potential |
title_full_unstemmed |
A non-hemorrhagic hybrid heparin/heparan sulfate with anticoagulant potential |
title_sort |
A non-hemorrhagic hybrid heparin/heparan sulfate with anticoagulant potential |
author |
Brito, Adriana S. |
author_facet |
Brito, Adriana S. Cavalcante, Romulo S. Palhares, Lais C. G. F. Hughes, Ashley J. Andrade, Giulianna P. V. Yates, Edwin Alexander [UNIFESP] Nader, Helena Bonciani [UNIFESP] Lima, Marcelo Andrade de [UNIFESP] Chavante, Suely F. |
author_role |
author |
author2 |
Cavalcante, Romulo S. Palhares, Lais C. G. F. Hughes, Ashley J. Andrade, Giulianna P. V. Yates, Edwin Alexander [UNIFESP] Nader, Helena Bonciani [UNIFESP] Lima, Marcelo Andrade de [UNIFESP] Chavante, Suely F. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Fed Rio Grande do Norte Univ Liverpool Diamond Light Source Ltd Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Brito, Adriana S. Cavalcante, Romulo S. Palhares, Lais C. G. F. Hughes, Ashley J. Andrade, Giulianna P. V. Yates, Edwin Alexander [UNIFESP] Nader, Helena Bonciani [UNIFESP] Lima, Marcelo Andrade de [UNIFESP] Chavante, Suely F. |
dc.subject.por.fl_str_mv |
Heparin Heparan sulfate Hemorrhagic activity Antithrombin Anticoagulant Thermostabilization |
topic |
Heparin Heparan sulfate Hemorrhagic activity Antithrombin Anticoagulant Thermostabilization |
description |
The structural characterization and the anticoagulant potential of a novel heparin/heparan sulfate-like compound from the heads of Litopenaeus vannamei shrimp are described. While it is distinct from either heparin or heparan sulfate, enzymatic depolymerization and nuclear magnetic resonance spectroscopy analyses revealed that this molecule does share some structural features with heparin, such as the high degree of N- and 6-0-sulfation and minor N-acetylation, and with heparan sulfate, in the glucuronic acid content. Its ability to stabilize human antithrombin explains its significant anticoagulant activity in aPTT and Factor-Xa inhibition assays. Interestingly, in contrast to mammalian heparin, the shrimp compound displayed negligible hemorrhagic effect. Together, these findings have particular interest since they reveal a novel molecule with significant anti-Xa activity coupled with low bleeding effects which make the shrimp heparin/HS-like compound a potential alternative for mammalian heparin. (C) 2013 Elsevier B.V. All rights reserved. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-02 2016-01-24T14:35:08Z 2016-01-24T14:35:08Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.carbpol.2013.08.063 Carbohydrate Polymers. Oxford: Elsevier B.V., v. 99, p. 372-378, 2014. 10.1016/j.carbpol.2013.08.063 WOS000329256600048.pdf 0144-8617 http://repositorio.unifesp.br/handle/11600/37305 WOS:000329256600048 |
url |
http://dx.doi.org/10.1016/j.carbpol.2013.08.063 http://repositorio.unifesp.br/handle/11600/37305 |
identifier_str_mv |
Carbohydrate Polymers. Oxford: Elsevier B.V., v. 99, p. 372-378, 2014. 10.1016/j.carbpol.2013.08.063 WOS000329256600048.pdf 0144-8617 WOS:000329256600048 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Carbohydrate Polymers |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
dc.format.none.fl_str_mv |
372-378 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268317675814912 |