A non-hemorrhagic hybrid heparin/heparan sulfate with anticoagulant potential

Detalhes bibliográficos
Autor(a) principal: Brito, Adriana S.
Data de Publicação: 2014
Outros Autores: Cavalcante, Romulo S., Palhares, Lais C. G. F., Hughes, Ashley J., Andrade, Giulianna P. V., Yates, Edwin Alexander [UNIFESP], Nader, Helena Bonciani [UNIFESP], Lima, Marcelo Andrade de [UNIFESP], Chavante, Suely F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1016/j.carbpol.2013.08.063
http://repositorio.unifesp.br/handle/11600/37305
Resumo: The structural characterization and the anticoagulant potential of a novel heparin/heparan sulfate-like compound from the heads of Litopenaeus vannamei shrimp are described. While it is distinct from either heparin or heparan sulfate, enzymatic depolymerization and nuclear magnetic resonance spectroscopy analyses revealed that this molecule does share some structural features with heparin, such as the high degree of N- and 6-0-sulfation and minor N-acetylation, and with heparan sulfate, in the glucuronic acid content. Its ability to stabilize human antithrombin explains its significant anticoagulant activity in aPTT and Factor-Xa inhibition assays. Interestingly, in contrast to mammalian heparin, the shrimp compound displayed negligible hemorrhagic effect. Together, these findings have particular interest since they reveal a novel molecule with significant anti-Xa activity coupled with low bleeding effects which make the shrimp heparin/HS-like compound a potential alternative for mammalian heparin. (C) 2013 Elsevier B.V. All rights reserved.
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spelling A non-hemorrhagic hybrid heparin/heparan sulfate with anticoagulant potentialHeparinHeparan sulfateHemorrhagic activityAntithrombinAnticoagulantThermostabilizationThe structural characterization and the anticoagulant potential of a novel heparin/heparan sulfate-like compound from the heads of Litopenaeus vannamei shrimp are described. While it is distinct from either heparin or heparan sulfate, enzymatic depolymerization and nuclear magnetic resonance spectroscopy analyses revealed that this molecule does share some structural features with heparin, such as the high degree of N- and 6-0-sulfation and minor N-acetylation, and with heparan sulfate, in the glucuronic acid content. Its ability to stabilize human antithrombin explains its significant anticoagulant activity in aPTT and Factor-Xa inhibition assays. Interestingly, in contrast to mammalian heparin, the shrimp compound displayed negligible hemorrhagic effect. Together, these findings have particular interest since they reveal a novel molecule with significant anti-Xa activity coupled with low bleeding effects which make the shrimp heparin/HS-like compound a potential alternative for mammalian heparin. (C) 2013 Elsevier B.V. All rights reserved.Univ Fed Rio Grande do Norte, Dept Bioquim, BR-59072970 Natal, RN, BrazilUniv Fed Rio Grande do Norte, Fac Ciencias Saude Trairi, Santa Cruz, RN, BrazilUniv Liverpool, Dept Struct & Chem Biol, Liverpool L69 7ZB, Merseyside, EnglandDiamond Light Source Ltd, Didcot OX11 0DE, Oxon, EnglandUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, BrazilWeb of SciencePPG.BIOQ (Programa de Pos-graduagao em Bioquimica/UFRN)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)LaisElsevier B.V.Univ Fed Rio Grande do NorteUniv LiverpoolDiamond Light Source LtdUniversidade Federal de São Paulo (UNIFESP)Brito, Adriana S.Cavalcante, Romulo S.Palhares, Lais C. G. F.Hughes, Ashley J.Andrade, Giulianna P. V.Yates, Edwin Alexander [UNIFESP]Nader, Helena Bonciani [UNIFESP]Lima, Marcelo Andrade de [UNIFESP]Chavante, Suely F.2016-01-24T14:35:08Z2016-01-24T14:35:08Z2014-01-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion372-378application/pdfhttp://dx.doi.org/10.1016/j.carbpol.2013.08.063Carbohydrate Polymers. Oxford: Elsevier B.V., v. 99, p. 372-378, 2014.10.1016/j.carbpol.2013.08.063WOS000329256600048.pdf0144-8617http://repositorio.unifesp.br/handle/11600/37305WOS:000329256600048engCarbohydrate Polymersinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-08T05:09:46Zoai:repositorio.unifesp.br/:11600/37305Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-08T05:09:46Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv A non-hemorrhagic hybrid heparin/heparan sulfate with anticoagulant potential
title A non-hemorrhagic hybrid heparin/heparan sulfate with anticoagulant potential
spellingShingle A non-hemorrhagic hybrid heparin/heparan sulfate with anticoagulant potential
Brito, Adriana S.
Heparin
Heparan sulfate
Hemorrhagic activity
Antithrombin
Anticoagulant
Thermostabilization
title_short A non-hemorrhagic hybrid heparin/heparan sulfate with anticoagulant potential
title_full A non-hemorrhagic hybrid heparin/heparan sulfate with anticoagulant potential
title_fullStr A non-hemorrhagic hybrid heparin/heparan sulfate with anticoagulant potential
title_full_unstemmed A non-hemorrhagic hybrid heparin/heparan sulfate with anticoagulant potential
title_sort A non-hemorrhagic hybrid heparin/heparan sulfate with anticoagulant potential
author Brito, Adriana S.
author_facet Brito, Adriana S.
Cavalcante, Romulo S.
Palhares, Lais C. G. F.
Hughes, Ashley J.
Andrade, Giulianna P. V.
Yates, Edwin Alexander [UNIFESP]
Nader, Helena Bonciani [UNIFESP]
Lima, Marcelo Andrade de [UNIFESP]
Chavante, Suely F.
author_role author
author2 Cavalcante, Romulo S.
Palhares, Lais C. G. F.
Hughes, Ashley J.
Andrade, Giulianna P. V.
Yates, Edwin Alexander [UNIFESP]
Nader, Helena Bonciani [UNIFESP]
Lima, Marcelo Andrade de [UNIFESP]
Chavante, Suely F.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Univ Fed Rio Grande do Norte
Univ Liverpool
Diamond Light Source Ltd
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Brito, Adriana S.
Cavalcante, Romulo S.
Palhares, Lais C. G. F.
Hughes, Ashley J.
Andrade, Giulianna P. V.
Yates, Edwin Alexander [UNIFESP]
Nader, Helena Bonciani [UNIFESP]
Lima, Marcelo Andrade de [UNIFESP]
Chavante, Suely F.
dc.subject.por.fl_str_mv Heparin
Heparan sulfate
Hemorrhagic activity
Antithrombin
Anticoagulant
Thermostabilization
topic Heparin
Heparan sulfate
Hemorrhagic activity
Antithrombin
Anticoagulant
Thermostabilization
description The structural characterization and the anticoagulant potential of a novel heparin/heparan sulfate-like compound from the heads of Litopenaeus vannamei shrimp are described. While it is distinct from either heparin or heparan sulfate, enzymatic depolymerization and nuclear magnetic resonance spectroscopy analyses revealed that this molecule does share some structural features with heparin, such as the high degree of N- and 6-0-sulfation and minor N-acetylation, and with heparan sulfate, in the glucuronic acid content. Its ability to stabilize human antithrombin explains its significant anticoagulant activity in aPTT and Factor-Xa inhibition assays. Interestingly, in contrast to mammalian heparin, the shrimp compound displayed negligible hemorrhagic effect. Together, these findings have particular interest since they reveal a novel molecule with significant anti-Xa activity coupled with low bleeding effects which make the shrimp heparin/HS-like compound a potential alternative for mammalian heparin. (C) 2013 Elsevier B.V. All rights reserved.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-02
2016-01-24T14:35:08Z
2016-01-24T14:35:08Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.carbpol.2013.08.063
Carbohydrate Polymers. Oxford: Elsevier B.V., v. 99, p. 372-378, 2014.
10.1016/j.carbpol.2013.08.063
WOS000329256600048.pdf
0144-8617
http://repositorio.unifesp.br/handle/11600/37305
WOS:000329256600048
url http://dx.doi.org/10.1016/j.carbpol.2013.08.063
http://repositorio.unifesp.br/handle/11600/37305
identifier_str_mv Carbohydrate Polymers. Oxford: Elsevier B.V., v. 99, p. 372-378, 2014.
10.1016/j.carbpol.2013.08.063
WOS000329256600048.pdf
0144-8617
WOS:000329256600048
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Carbohydrate Polymers
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.format.none.fl_str_mv 372-378
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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