Análise comparativa de ampolas e frascos-ampolas em embalagens estéreis e convencionais quanto a carga microbiana e teste de esterilidade

• Autor(a) principal: Freitas, Raphael Ribeiro de Aquino [UNIFESP]
• Data de Publicação: 2016
• Outros Autores: Tardelli, Maria Angela [UNIFESP]
• Tipo de documento: Artigo
• Idioma: eng; por
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.1590/S1679-45082016AO3484; https://repositorio.unifesp.br/handle/11600/56071
• topic: Infection control; Anesthesia; Contamination; Anesthesia, conduction; Controle de infecções; Anestesia; Contaminação; Anestesia por condução

Objective: To compare sterility and microbial (bacteria and fungi) load in the outer part of hyperbaric bupivacaine (Neocaina (R)) in ampoule and bupivacaine in vial, in conventional and sterile pack formulations. Methods: The sterile packs were divided into two groups: G1 (n=16) with ampoules and G2 (n=16) with vials. Conventional formulations were divided into two groups, being G3 (n=16) with ampoules and G4 (n=16) with vials. The ampoules and vials were opened and had their content drawn. The empty bottles were then placed in sterile plastic bags and sent for analysis of microbial load (bacteria and fungi) and sterility testing. Data were analyzed using the chi(2) test with Yates correction, and 95% confidence interval. Results: G1 and G2 showed no bacterial growth when compared to conventional groups (p<0.001). The most common agent in conventional microbiological samples was Staphylococcus aureus. There was no fungal growth in both groups. Conclusion: The use of (sterile pack) reduces the microbial load of bottles, and would decrease the chance of exposure to potential contamination of the anesthetic solution.