Long-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn rats

Detalhes bibliográficos
Autor(a) principal: Romariz, Simone Amaro Alves [UNIFESP]
Data de Publicação: 2014
Outros Autores: Paiva, Daisylea de Souza [UNIFESP], Valente, Maria Fernanda [UNIFESP], Barnabe, Gabriela Filoso [UNIFESP], Frussa-Filho, Roberto [UNIFESP], Silva, Regina Cláudia Barbosa da [UNIFESP], Calcagnotto, Maria Elisa, Longo, Beatriz Monteiro [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: https://dx.doi.org/10.1186/1471-2202-15-94
https://repositorio.unifesp.br/handle/11600/38077
Resumo: Background: the GABAergic system plays an important role in modulating levels of anxiety. When transplanted into the brain, precursor cells from the medial ganglionic eminence (MGE) have the ability to differentiate into GABAergic interneurons and modify the inhibitory tone in the host brain. Currently, two methods have been reported for obtaining MGE precursor cells for transplantation: fresh and neurosphere dissociated cells. Here, we investigated the effects generated by transplantation of the two types of cell preparations on anxiety behavior in rats.Results: We transplanted freshly dissociated or neurosphere dissociated cells into the neonate brain of male rats on postnatal (PN) day 2-3. At early adulthood (PN 62-63), transplanted animals were tested in the Elevated Plus Maze (EPM). To verify the differentiation and migration pattern of the transplanted cells in vitro and in vivo, we performed immunohistochemistry for GFP and several interneuron-specific markers: neuropeptide Y (NPY), parvalbumin (PV) and calretinin (CR). Cells from both types of preparations expressed these interneuronal markers. However, an anxiolytic effect on behavior in the EPM was observed in animals that received the MGE-derived freshly dissociated cells but not in those that received the neurosphere dissociated cells.Conclusion: Our results suggest a long-lasting anxiolytic effect of transplanted freshly dissociated cells that reinforces the inhibitory function of the GABAergic neuronal circuitry in the hippocampus related to anxiety-like behavior in rats.
id UFSP_9d5105410e0eba1ee7d4aff7caff785e
oai_identifier_str oai:repositorio.unifesp.br/:11600/38077
network_acronym_str UFSP
network_name_str Repositório Institucional da UNIFESP
repository_id_str 3465
spelling Long-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn ratsAnxietyMedial ganglionic eminenceNeuronal precursor cellsTransplantationBackground: the GABAergic system plays an important role in modulating levels of anxiety. When transplanted into the brain, precursor cells from the medial ganglionic eminence (MGE) have the ability to differentiate into GABAergic interneurons and modify the inhibitory tone in the host brain. Currently, two methods have been reported for obtaining MGE precursor cells for transplantation: fresh and neurosphere dissociated cells. Here, we investigated the effects generated by transplantation of the two types of cell preparations on anxiety behavior in rats.Results: We transplanted freshly dissociated or neurosphere dissociated cells into the neonate brain of male rats on postnatal (PN) day 2-3. At early adulthood (PN 62-63), transplanted animals were tested in the Elevated Plus Maze (EPM). To verify the differentiation and migration pattern of the transplanted cells in vitro and in vivo, we performed immunohistochemistry for GFP and several interneuron-specific markers: neuropeptide Y (NPY), parvalbumin (PV) and calretinin (CR). Cells from both types of preparations expressed these interneuronal markers. However, an anxiolytic effect on behavior in the EPM was observed in animals that received the MGE-derived freshly dissociated cells but not in those that received the neurosphere dissociated cells.Conclusion: Our results suggest a long-lasting anxiolytic effect of transplanted freshly dissociated cells that reinforces the inhibitory function of the GABAergic neuronal circuitry in the hippocampus related to anxiety-like behavior in rats.Universidade Federal de São Paulo, Dept Fisiol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Farmacol, BR-04023062 São Paulo, BrazilUNIFESP, Dept Biociencias, BR-11015020 Santos, SP, BrazilUniv Fed Rio Grande do Sul, Inst Ciencias Basicas Saude, Dept Bioquim, BR-90035003 Porto Alegre, RS, BrazilUniversidade Federal de São Paulo, Dept Fisiol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Farmacol, BR-04023062 São Paulo, BrazilUNIFESP, Dept Biociencias, BR-11015020 Santos, SP, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Biomed Central LtdUniversidade Federal de São Paulo (UNIFESP)Univ Fed Rio Grande do SulRomariz, Simone Amaro Alves [UNIFESP]Paiva, Daisylea de Souza [UNIFESP]Valente, Maria Fernanda [UNIFESP]Barnabe, Gabriela Filoso [UNIFESP]Frussa-Filho, Roberto [UNIFESP]Silva, Regina Cláudia Barbosa da [UNIFESP]Calcagnotto, Maria ElisaLongo, Beatriz Monteiro [UNIFESP]2016-01-24T14:37:42Z2016-01-24T14:37:42Z2014-08-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion8application/pdfhttps://dx.doi.org/10.1186/1471-2202-15-94Bmc Neuroscience. London: Biomed Central Ltd, v. 15, 8 p., 2014.10.1186/1471-2202-15-94WOS000341598500001.pdf1471-2202https://repositorio.unifesp.br/handle/11600/38077WOS:000341598500001engBmc Neuroscienceinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-01T03:40:30Zoai:repositorio.unifesp.br/:11600/38077Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-01T03:40:30Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Long-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn rats
title Long-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn rats
spellingShingle Long-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn rats
Romariz, Simone Amaro Alves [UNIFESP]
Anxiety
Medial ganglionic eminence
Neuronal precursor cells
Transplantation
title_short Long-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn rats
title_full Long-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn rats
title_fullStr Long-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn rats
title_full_unstemmed Long-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn rats
title_sort Long-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn rats
author Romariz, Simone Amaro Alves [UNIFESP]
author_facet Romariz, Simone Amaro Alves [UNIFESP]
Paiva, Daisylea de Souza [UNIFESP]
Valente, Maria Fernanda [UNIFESP]
Barnabe, Gabriela Filoso [UNIFESP]
Frussa-Filho, Roberto [UNIFESP]
Silva, Regina Cláudia Barbosa da [UNIFESP]
Calcagnotto, Maria Elisa
Longo, Beatriz Monteiro [UNIFESP]
author_role author
author2 Paiva, Daisylea de Souza [UNIFESP]
Valente, Maria Fernanda [UNIFESP]
Barnabe, Gabriela Filoso [UNIFESP]
Frussa-Filho, Roberto [UNIFESP]
Silva, Regina Cláudia Barbosa da [UNIFESP]
Calcagnotto, Maria Elisa
Longo, Beatriz Monteiro [UNIFESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Univ Fed Rio Grande do Sul
dc.contributor.author.fl_str_mv Romariz, Simone Amaro Alves [UNIFESP]
Paiva, Daisylea de Souza [UNIFESP]
Valente, Maria Fernanda [UNIFESP]
Barnabe, Gabriela Filoso [UNIFESP]
Frussa-Filho, Roberto [UNIFESP]
Silva, Regina Cláudia Barbosa da [UNIFESP]
Calcagnotto, Maria Elisa
Longo, Beatriz Monteiro [UNIFESP]
dc.subject.por.fl_str_mv Anxiety
Medial ganglionic eminence
Neuronal precursor cells
Transplantation
topic Anxiety
Medial ganglionic eminence
Neuronal precursor cells
Transplantation
description Background: the GABAergic system plays an important role in modulating levels of anxiety. When transplanted into the brain, precursor cells from the medial ganglionic eminence (MGE) have the ability to differentiate into GABAergic interneurons and modify the inhibitory tone in the host brain. Currently, two methods have been reported for obtaining MGE precursor cells for transplantation: fresh and neurosphere dissociated cells. Here, we investigated the effects generated by transplantation of the two types of cell preparations on anxiety behavior in rats.Results: We transplanted freshly dissociated or neurosphere dissociated cells into the neonate brain of male rats on postnatal (PN) day 2-3. At early adulthood (PN 62-63), transplanted animals were tested in the Elevated Plus Maze (EPM). To verify the differentiation and migration pattern of the transplanted cells in vitro and in vivo, we performed immunohistochemistry for GFP and several interneuron-specific markers: neuropeptide Y (NPY), parvalbumin (PV) and calretinin (CR). Cells from both types of preparations expressed these interneuronal markers. However, an anxiolytic effect on behavior in the EPM was observed in animals that received the MGE-derived freshly dissociated cells but not in those that received the neurosphere dissociated cells.Conclusion: Our results suggest a long-lasting anxiolytic effect of transplanted freshly dissociated cells that reinforces the inhibitory function of the GABAergic neuronal circuitry in the hippocampus related to anxiety-like behavior in rats.
publishDate 2014
dc.date.none.fl_str_mv 2014-08-02
2016-01-24T14:37:42Z
2016-01-24T14:37:42Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://dx.doi.org/10.1186/1471-2202-15-94
Bmc Neuroscience. London: Biomed Central Ltd, v. 15, 8 p., 2014.
10.1186/1471-2202-15-94
WOS000341598500001.pdf
1471-2202
https://repositorio.unifesp.br/handle/11600/38077
WOS:000341598500001
url https://dx.doi.org/10.1186/1471-2202-15-94
https://repositorio.unifesp.br/handle/11600/38077
identifier_str_mv Bmc Neuroscience. London: Biomed Central Ltd, v. 15, 8 p., 2014.
10.1186/1471-2202-15-94
WOS000341598500001.pdf
1471-2202
WOS:000341598500001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Bmc Neuroscience
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 8
application/pdf
dc.publisher.none.fl_str_mv Biomed Central Ltd
publisher.none.fl_str_mv Biomed Central Ltd
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268399368273920