Long-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn rats
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://dx.doi.org/10.1186/1471-2202-15-94 https://repositorio.unifesp.br/handle/11600/38077 |
Resumo: | Background: the GABAergic system plays an important role in modulating levels of anxiety. When transplanted into the brain, precursor cells from the medial ganglionic eminence (MGE) have the ability to differentiate into GABAergic interneurons and modify the inhibitory tone in the host brain. Currently, two methods have been reported for obtaining MGE precursor cells for transplantation: fresh and neurosphere dissociated cells. Here, we investigated the effects generated by transplantation of the two types of cell preparations on anxiety behavior in rats.Results: We transplanted freshly dissociated or neurosphere dissociated cells into the neonate brain of male rats on postnatal (PN) day 2-3. At early adulthood (PN 62-63), transplanted animals were tested in the Elevated Plus Maze (EPM). To verify the differentiation and migration pattern of the transplanted cells in vitro and in vivo, we performed immunohistochemistry for GFP and several interneuron-specific markers: neuropeptide Y (NPY), parvalbumin (PV) and calretinin (CR). Cells from both types of preparations expressed these interneuronal markers. However, an anxiolytic effect on behavior in the EPM was observed in animals that received the MGE-derived freshly dissociated cells but not in those that received the neurosphere dissociated cells.Conclusion: Our results suggest a long-lasting anxiolytic effect of transplanted freshly dissociated cells that reinforces the inhibitory function of the GABAergic neuronal circuitry in the hippocampus related to anxiety-like behavior in rats. |
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Long-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn ratsAnxietyMedial ganglionic eminenceNeuronal precursor cellsTransplantationBackground: the GABAergic system plays an important role in modulating levels of anxiety. When transplanted into the brain, precursor cells from the medial ganglionic eminence (MGE) have the ability to differentiate into GABAergic interneurons and modify the inhibitory tone in the host brain. Currently, two methods have been reported for obtaining MGE precursor cells for transplantation: fresh and neurosphere dissociated cells. Here, we investigated the effects generated by transplantation of the two types of cell preparations on anxiety behavior in rats.Results: We transplanted freshly dissociated or neurosphere dissociated cells into the neonate brain of male rats on postnatal (PN) day 2-3. At early adulthood (PN 62-63), transplanted animals were tested in the Elevated Plus Maze (EPM). To verify the differentiation and migration pattern of the transplanted cells in vitro and in vivo, we performed immunohistochemistry for GFP and several interneuron-specific markers: neuropeptide Y (NPY), parvalbumin (PV) and calretinin (CR). Cells from both types of preparations expressed these interneuronal markers. However, an anxiolytic effect on behavior in the EPM was observed in animals that received the MGE-derived freshly dissociated cells but not in those that received the neurosphere dissociated cells.Conclusion: Our results suggest a long-lasting anxiolytic effect of transplanted freshly dissociated cells that reinforces the inhibitory function of the GABAergic neuronal circuitry in the hippocampus related to anxiety-like behavior in rats.Universidade Federal de São Paulo, Dept Fisiol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Farmacol, BR-04023062 São Paulo, BrazilUNIFESP, Dept Biociencias, BR-11015020 Santos, SP, BrazilUniv Fed Rio Grande do Sul, Inst Ciencias Basicas Saude, Dept Bioquim, BR-90035003 Porto Alegre, RS, BrazilUniversidade Federal de São Paulo, Dept Fisiol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Farmacol, BR-04023062 São Paulo, BrazilUNIFESP, Dept Biociencias, BR-11015020 Santos, SP, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Biomed Central LtdUniversidade Federal de São Paulo (UNIFESP)Univ Fed Rio Grande do SulRomariz, Simone Amaro Alves [UNIFESP]Paiva, Daisylea de Souza [UNIFESP]Valente, Maria Fernanda [UNIFESP]Barnabe, Gabriela Filoso [UNIFESP]Frussa-Filho, Roberto [UNIFESP]Silva, Regina Cláudia Barbosa da [UNIFESP]Calcagnotto, Maria ElisaLongo, Beatriz Monteiro [UNIFESP]2016-01-24T14:37:42Z2016-01-24T14:37:42Z2014-08-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion8application/pdfhttps://dx.doi.org/10.1186/1471-2202-15-94Bmc Neuroscience. London: Biomed Central Ltd, v. 15, 8 p., 2014.10.1186/1471-2202-15-94WOS000341598500001.pdf1471-2202https://repositorio.unifesp.br/handle/11600/38077WOS:000341598500001engBmc Neuroscienceinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-01T03:40:30Zoai:repositorio.unifesp.br/:11600/38077Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-01T03:40:30Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Long-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn rats |
title |
Long-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn rats |
spellingShingle |
Long-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn rats Romariz, Simone Amaro Alves [UNIFESP] Anxiety Medial ganglionic eminence Neuronal precursor cells Transplantation |
title_short |
Long-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn rats |
title_full |
Long-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn rats |
title_fullStr |
Long-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn rats |
title_full_unstemmed |
Long-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn rats |
title_sort |
Long-lasting anxiolytic effect of neural precursor cells freshly prepared but not neurosphere-derived cell transplantation in newborn rats |
author |
Romariz, Simone Amaro Alves [UNIFESP] |
author_facet |
Romariz, Simone Amaro Alves [UNIFESP] Paiva, Daisylea de Souza [UNIFESP] Valente, Maria Fernanda [UNIFESP] Barnabe, Gabriela Filoso [UNIFESP] Frussa-Filho, Roberto [UNIFESP] Silva, Regina Cláudia Barbosa da [UNIFESP] Calcagnotto, Maria Elisa Longo, Beatriz Monteiro [UNIFESP] |
author_role |
author |
author2 |
Paiva, Daisylea de Souza [UNIFESP] Valente, Maria Fernanda [UNIFESP] Barnabe, Gabriela Filoso [UNIFESP] Frussa-Filho, Roberto [UNIFESP] Silva, Regina Cláudia Barbosa da [UNIFESP] Calcagnotto, Maria Elisa Longo, Beatriz Monteiro [UNIFESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Univ Fed Rio Grande do Sul |
dc.contributor.author.fl_str_mv |
Romariz, Simone Amaro Alves [UNIFESP] Paiva, Daisylea de Souza [UNIFESP] Valente, Maria Fernanda [UNIFESP] Barnabe, Gabriela Filoso [UNIFESP] Frussa-Filho, Roberto [UNIFESP] Silva, Regina Cláudia Barbosa da [UNIFESP] Calcagnotto, Maria Elisa Longo, Beatriz Monteiro [UNIFESP] |
dc.subject.por.fl_str_mv |
Anxiety Medial ganglionic eminence Neuronal precursor cells Transplantation |
topic |
Anxiety Medial ganglionic eminence Neuronal precursor cells Transplantation |
description |
Background: the GABAergic system plays an important role in modulating levels of anxiety. When transplanted into the brain, precursor cells from the medial ganglionic eminence (MGE) have the ability to differentiate into GABAergic interneurons and modify the inhibitory tone in the host brain. Currently, two methods have been reported for obtaining MGE precursor cells for transplantation: fresh and neurosphere dissociated cells. Here, we investigated the effects generated by transplantation of the two types of cell preparations on anxiety behavior in rats.Results: We transplanted freshly dissociated or neurosphere dissociated cells into the neonate brain of male rats on postnatal (PN) day 2-3. At early adulthood (PN 62-63), transplanted animals were tested in the Elevated Plus Maze (EPM). To verify the differentiation and migration pattern of the transplanted cells in vitro and in vivo, we performed immunohistochemistry for GFP and several interneuron-specific markers: neuropeptide Y (NPY), parvalbumin (PV) and calretinin (CR). Cells from both types of preparations expressed these interneuronal markers. However, an anxiolytic effect on behavior in the EPM was observed in animals that received the MGE-derived freshly dissociated cells but not in those that received the neurosphere dissociated cells.Conclusion: Our results suggest a long-lasting anxiolytic effect of transplanted freshly dissociated cells that reinforces the inhibitory function of the GABAergic neuronal circuitry in the hippocampus related to anxiety-like behavior in rats. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-08-02 2016-01-24T14:37:42Z 2016-01-24T14:37:42Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://dx.doi.org/10.1186/1471-2202-15-94 Bmc Neuroscience. London: Biomed Central Ltd, v. 15, 8 p., 2014. 10.1186/1471-2202-15-94 WOS000341598500001.pdf 1471-2202 https://repositorio.unifesp.br/handle/11600/38077 WOS:000341598500001 |
url |
https://dx.doi.org/10.1186/1471-2202-15-94 https://repositorio.unifesp.br/handle/11600/38077 |
identifier_str_mv |
Bmc Neuroscience. London: Biomed Central Ltd, v. 15, 8 p., 2014. 10.1186/1471-2202-15-94 WOS000341598500001.pdf 1471-2202 WOS:000341598500001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bmc Neuroscience |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd |
publisher.none.fl_str_mv |
Biomed Central Ltd |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268399368273920 |