Palmitoleic acid (n-7) increases white adipocyte lipolysis and lipase content in a PPAR alpha-dependent manner

• Autor(a) principal: Bolsoni-Lopes, Andressa
• Data de Publicação: 2013
• Outros Autores: Festuccia, William T., Farias, Talita S. M., Chimin, Patricia, Torres-Leal, Francisco L., Derogis, Priscilla B. M., Andrade, Paula B. de, Miyamoto, Sayuri, Lima, Fabio B., Curi, Rui, Alonso-Vale, Maria Isabel C. [UNIFESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.1152/ajpendo.00082.2013; http://repositorio.unifesp.br/handle/11600/36907
• topic: ATGL; HSL; lipogenesis; triacylglycerol/fatty acid cycle

We investigated whether palmitoleic acid, a fatty acid that enhances whole body glucose disposal and suppresses hepatic steatosis, modulates triacylglycerol (TAG) metabolism in adipocytes. for this, both differentiated 3T3-L1 cells treated with either palmitoleic acid (16: 1n7, 200 mu M) or palmitic acid (16: 0, 200 mu M) for 24 h and primary adipocytes from wild-type or PPAR alpha-deficient mice treated with 16: 1n7 (300 mg.kg (-1.)day (-1)) or oleic acid (18: 1n9, 300 mg.kg (-1).day (-1)) by gavage for 10 days were evaluated for lipolysis, TAG, and glycerol 3-phosphate synthesis and gene and protein expression profile. Treatment of differentiated 3T3-L1 cells with 16:1n7, but not 16: 0, increased basal and isoproterenol-stimulated lipolysis, mRNA levels of adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) and protein content of ATGL and pSer(660)-HSL. Such increase in lipolysis induced by 16: 1n7, which can be prevented by pharmacological inhibition of PPAR alpha, was associated with higher rates of PPAR alpha binding to DNA. in contrast to lipolysis, both 16: 1n7 and 16: 0 increased fatty acid incorporation into TAG and glycerol 3-phosphate synthesis from glucose without affecting glyceroneogenesis and glycerokinase expression. Corroborating in vitro findings, treatment of wild-type but not PPAR alpha-deficient mice with 16: 1n7 increased primary adipocyte basal and stimulated lipolysis and ATGL and HSL mRNA levels. in contrast to lipolysis, however, 16: 1n7 treatment increased fatty acid incorporation into TAG and glycerol 3-phosphate synthesis from glucose in both wild-type and PPAR alpha-deficient mice. in conclusion, palmitoleic acid increases adipocyte lipolysis and lipases by a mechanism that requires a functional PPAR alpha.