Efeitos do exercício físico na expressão e atividade da AMPKα em ratos obesos induzidos por dieta rica em gordura
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://dx.doi.org/10.1590/S1517-86922009000200003 https://repositorio.unifesp.br/handle/11600/45256 |
Resumo: | Introduction: High-fat diet is a special risk factor in the development of insulin resistance and type 2 diabetes. Objective: To investigate the effects of physical exercise on the AMPK expression and activity in high-fat diet induced obese rats. Methods: Wistar rats were randomly divided into four groups and received either a rat maintenance diet (control group) or an isocaloric high-fat diet (HFD) (sedentary groups and exercised groups) for four months. Two different exercise protocols were utilized: acute or chronic swimming exercise. Insulin tolerance test was performed to estimate whole-body insulin sensitivity. AMPK alpha and GLUT4 as well as pAMPK alpha and pACC of rats' skeletal muscle levels were determined using Western blot. Results: Insulin tolerance test revealed a significantly impaired insulin action after HFDt feeding, indicating high-fat induced insulin resistance when compared to control group. Four months of HFD treatment induced to significant decrease of AMPKa (2.2-fold) and GLUT4 (2.5-fold) protein contents and also of p-AMPK alpha (2.4-fold) and p-ACC (2.5-fold) in sedentary rats' skeletal muscle when compared with the control group. Both exercise protocols resulted in increase of AMPKa and ACC phosphorylation and increase in insulin sensitivity, while chronic physical exercise alone provoked increase in these proteins expression (p < 0.05). Conclusion: High-fat feeding impairs AMPKa activity, while AMPKa activation by physical exercise improves insulin resistance, thus indicating that obese rats normally have the AMPK pathway preserved. |
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Efeitos do exercício físico na expressão e atividade da AMPKα em ratos obesos induzidos por dieta rica em gorduraEffects of Physical Exercise in the Ampk alpha Expression and Activity in High-fat Diet Induced Obese RatsObesityInsulin resistancePhysical education and trainingIntroduction: High-fat diet is a special risk factor in the development of insulin resistance and type 2 diabetes. Objective: To investigate the effects of physical exercise on the AMPK expression and activity in high-fat diet induced obese rats. Methods: Wistar rats were randomly divided into four groups and received either a rat maintenance diet (control group) or an isocaloric high-fat diet (HFD) (sedentary groups and exercised groups) for four months. Two different exercise protocols were utilized: acute or chronic swimming exercise. Insulin tolerance test was performed to estimate whole-body insulin sensitivity. AMPK alpha and GLUT4 as well as pAMPK alpha and pACC of rats' skeletal muscle levels were determined using Western blot. Results: Insulin tolerance test revealed a significantly impaired insulin action after HFDt feeding, indicating high-fat induced insulin resistance when compared to control group. Four months of HFD treatment induced to significant decrease of AMPKa (2.2-fold) and GLUT4 (2.5-fold) protein contents and also of p-AMPK alpha (2.4-fold) and p-ACC (2.5-fold) in sedentary rats' skeletal muscle when compared with the control group. Both exercise protocols resulted in increase of AMPKa and ACC phosphorylation and increase in insulin sensitivity, while chronic physical exercise alone provoked increase in these proteins expression (p < 0.05). Conclusion: High-fat feeding impairs AMPKa activity, while AMPKa activation by physical exercise improves insulin resistance, thus indicating that obese rats normally have the AMPK pathway preserved.Univ Estadual Campinas, UNICAMP, Dept Clin Med, FCM, Campinas, SP, BrazilUniv Fed Sao Paulo, Fac Educ Fis Modalidade Saude, Dept Biociencias, Santos, SP, BrazilUniv Fed Sao Paulo, Fac Educ Fis Modalidade Saude, Dept Biociencias, Santos, SP, BrazilWeb of ScienceSoc Brasileira Med EsporteUniversidade Estadual de Campinas (UNICAMP)Universidade Federal de São Paulo (UNIFESP)Pauli, José Rodrigo [UNIFESP]Ropelle, Eduardo RocheteCintra, Dennys EsperSouza, Claudio Teodoro de2018-06-18T11:35:44Z2018-06-18T11:35:44Z2009-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion98-103application/pdfhttps://dx.doi.org/10.1590/S1517-86922009000200003Revista Brasileira De Medicina Do Esporte. Sao Paulo Sp: Soc Brasileira Med Esporte, v. 15, n. 2, p. 98-103, 2009.10.1590/S1517-86922009000200003S1517-86922009000200003.pdf1517-8692S1517-86922009000200003https://repositorio.unifesp.br/handle/11600/45256WOS:000266133400003porRevista Brasileira De Medicina Do Esporteinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-10T00:16:49Zoai:repositorio.unifesp.br/:11600/45256Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-10T00:16:49Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Efeitos do exercício físico na expressão e atividade da AMPKα em ratos obesos induzidos por dieta rica em gordura Effects of Physical Exercise in the Ampk alpha Expression and Activity in High-fat Diet Induced Obese Rats |
title |
Efeitos do exercício físico na expressão e atividade da AMPKα em ratos obesos induzidos por dieta rica em gordura |
spellingShingle |
Efeitos do exercício físico na expressão e atividade da AMPKα em ratos obesos induzidos por dieta rica em gordura Pauli, José Rodrigo [UNIFESP] Obesity Insulin resistance Physical education and training |
title_short |
Efeitos do exercício físico na expressão e atividade da AMPKα em ratos obesos induzidos por dieta rica em gordura |
title_full |
Efeitos do exercício físico na expressão e atividade da AMPKα em ratos obesos induzidos por dieta rica em gordura |
title_fullStr |
Efeitos do exercício físico na expressão e atividade da AMPKα em ratos obesos induzidos por dieta rica em gordura |
title_full_unstemmed |
Efeitos do exercício físico na expressão e atividade da AMPKα em ratos obesos induzidos por dieta rica em gordura |
title_sort |
Efeitos do exercício físico na expressão e atividade da AMPKα em ratos obesos induzidos por dieta rica em gordura |
author |
Pauli, José Rodrigo [UNIFESP] |
author_facet |
Pauli, José Rodrigo [UNIFESP] Ropelle, Eduardo Rochete Cintra, Dennys Esper Souza, Claudio Teodoro de |
author_role |
author |
author2 |
Ropelle, Eduardo Rochete Cintra, Dennys Esper Souza, Claudio Teodoro de |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Pauli, José Rodrigo [UNIFESP] Ropelle, Eduardo Rochete Cintra, Dennys Esper Souza, Claudio Teodoro de |
dc.subject.por.fl_str_mv |
Obesity Insulin resistance Physical education and training |
topic |
Obesity Insulin resistance Physical education and training |
description |
Introduction: High-fat diet is a special risk factor in the development of insulin resistance and type 2 diabetes. Objective: To investigate the effects of physical exercise on the AMPK expression and activity in high-fat diet induced obese rats. Methods: Wistar rats were randomly divided into four groups and received either a rat maintenance diet (control group) or an isocaloric high-fat diet (HFD) (sedentary groups and exercised groups) for four months. Two different exercise protocols were utilized: acute or chronic swimming exercise. Insulin tolerance test was performed to estimate whole-body insulin sensitivity. AMPK alpha and GLUT4 as well as pAMPK alpha and pACC of rats' skeletal muscle levels were determined using Western blot. Results: Insulin tolerance test revealed a significantly impaired insulin action after HFDt feeding, indicating high-fat induced insulin resistance when compared to control group. Four months of HFD treatment induced to significant decrease of AMPKa (2.2-fold) and GLUT4 (2.5-fold) protein contents and also of p-AMPK alpha (2.4-fold) and p-ACC (2.5-fold) in sedentary rats' skeletal muscle when compared with the control group. Both exercise protocols resulted in increase of AMPKa and ACC phosphorylation and increase in insulin sensitivity, while chronic physical exercise alone provoked increase in these proteins expression (p < 0.05). Conclusion: High-fat feeding impairs AMPKa activity, while AMPKa activation by physical exercise improves insulin resistance, thus indicating that obese rats normally have the AMPK pathway preserved. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-03-01 2018-06-18T11:35:44Z 2018-06-18T11:35:44Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://dx.doi.org/10.1590/S1517-86922009000200003 Revista Brasileira De Medicina Do Esporte. Sao Paulo Sp: Soc Brasileira Med Esporte, v. 15, n. 2, p. 98-103, 2009. 10.1590/S1517-86922009000200003 S1517-86922009000200003.pdf 1517-8692 S1517-86922009000200003 https://repositorio.unifesp.br/handle/11600/45256 WOS:000266133400003 |
url |
https://dx.doi.org/10.1590/S1517-86922009000200003 https://repositorio.unifesp.br/handle/11600/45256 |
identifier_str_mv |
Revista Brasileira De Medicina Do Esporte. Sao Paulo Sp: Soc Brasileira Med Esporte, v. 15, n. 2, p. 98-103, 2009. 10.1590/S1517-86922009000200003 S1517-86922009000200003.pdf 1517-8692 S1517-86922009000200003 WOS:000266133400003 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Revista Brasileira De Medicina Do Esporte |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
98-103 application/pdf |
dc.publisher.none.fl_str_mv |
Soc Brasileira Med Esporte |
publisher.none.fl_str_mv |
Soc Brasileira Med Esporte |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1814268393968107520 |