Efeitos do exercício físico na expressão e atividade da AMPKα em ratos obesos induzidos por dieta rica em gordura

Detalhes bibliográficos
Autor(a) principal: Pauli, José Rodrigo [UNIFESP]
Data de Publicação: 2009
Outros Autores: Ropelle, Eduardo Rochete, Cintra, Dennys Esper, Souza, Claudio Teodoro de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: https://dx.doi.org/10.1590/S1517-86922009000200003
https://repositorio.unifesp.br/handle/11600/45256
Resumo: Introduction: High-fat diet is a special risk factor in the development of insulin resistance and type 2 diabetes. Objective: To investigate the effects of physical exercise on the AMPK expression and activity in high-fat diet induced obese rats. Methods: Wistar rats were randomly divided into four groups and received either a rat maintenance diet (control group) or an isocaloric high-fat diet (HFD) (sedentary groups and exercised groups) for four months. Two different exercise protocols were utilized: acute or chronic swimming exercise. Insulin tolerance test was performed to estimate whole-body insulin sensitivity. AMPK alpha and GLUT4 as well as pAMPK alpha and pACC of rats' skeletal muscle levels were determined using Western blot. Results: Insulin tolerance test revealed a significantly impaired insulin action after HFDt feeding, indicating high-fat induced insulin resistance when compared to control group. Four months of HFD treatment induced to significant decrease of AMPKa (2.2-fold) and GLUT4 (2.5-fold) protein contents and also of p-AMPK alpha (2.4-fold) and p-ACC (2.5-fold) in sedentary rats' skeletal muscle when compared with the control group. Both exercise protocols resulted in increase of AMPKa and ACC phosphorylation and increase in insulin sensitivity, while chronic physical exercise alone provoked increase in these proteins expression (p < 0.05). Conclusion: High-fat feeding impairs AMPKa activity, while AMPKa activation by physical exercise improves insulin resistance, thus indicating that obese rats normally have the AMPK pathway preserved.
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spelling Efeitos do exercício físico na expressão e atividade da AMPKα em ratos obesos induzidos por dieta rica em gorduraEffects of Physical Exercise in the Ampk alpha Expression and Activity in High-fat Diet Induced Obese RatsObesityInsulin resistancePhysical education and trainingIntroduction: High-fat diet is a special risk factor in the development of insulin resistance and type 2 diabetes. Objective: To investigate the effects of physical exercise on the AMPK expression and activity in high-fat diet induced obese rats. Methods: Wistar rats were randomly divided into four groups and received either a rat maintenance diet (control group) or an isocaloric high-fat diet (HFD) (sedentary groups and exercised groups) for four months. Two different exercise protocols were utilized: acute or chronic swimming exercise. Insulin tolerance test was performed to estimate whole-body insulin sensitivity. AMPK alpha and GLUT4 as well as pAMPK alpha and pACC of rats' skeletal muscle levels were determined using Western blot. Results: Insulin tolerance test revealed a significantly impaired insulin action after HFDt feeding, indicating high-fat induced insulin resistance when compared to control group. Four months of HFD treatment induced to significant decrease of AMPKa (2.2-fold) and GLUT4 (2.5-fold) protein contents and also of p-AMPK alpha (2.4-fold) and p-ACC (2.5-fold) in sedentary rats' skeletal muscle when compared with the control group. Both exercise protocols resulted in increase of AMPKa and ACC phosphorylation and increase in insulin sensitivity, while chronic physical exercise alone provoked increase in these proteins expression (p < 0.05). Conclusion: High-fat feeding impairs AMPKa activity, while AMPKa activation by physical exercise improves insulin resistance, thus indicating that obese rats normally have the AMPK pathway preserved.Univ Estadual Campinas, UNICAMP, Dept Clin Med, FCM, Campinas, SP, BrazilUniv Fed Sao Paulo, Fac Educ Fis Modalidade Saude, Dept Biociencias, Santos, SP, BrazilUniv Fed Sao Paulo, Fac Educ Fis Modalidade Saude, Dept Biociencias, Santos, SP, BrazilWeb of ScienceSoc Brasileira Med EsporteUniversidade Estadual de Campinas (UNICAMP)Universidade Federal de São Paulo (UNIFESP)Pauli, José Rodrigo [UNIFESP]Ropelle, Eduardo RocheteCintra, Dennys EsperSouza, Claudio Teodoro de2018-06-18T11:35:44Z2018-06-18T11:35:44Z2009-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion98-103application/pdfhttps://dx.doi.org/10.1590/S1517-86922009000200003Revista Brasileira De Medicina Do Esporte. Sao Paulo Sp: Soc Brasileira Med Esporte, v. 15, n. 2, p. 98-103, 2009.10.1590/S1517-86922009000200003S1517-86922009000200003.pdf1517-8692S1517-86922009000200003https://repositorio.unifesp.br/handle/11600/45256WOS:000266133400003porRevista Brasileira De Medicina Do Esporteinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-10T00:16:49Zoai:repositorio.unifesp.br/:11600/45256Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-10T00:16:49Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Efeitos do exercício físico na expressão e atividade da AMPKα em ratos obesos induzidos por dieta rica em gordura
Effects of Physical Exercise in the Ampk alpha Expression and Activity in High-fat Diet Induced Obese Rats
title Efeitos do exercício físico na expressão e atividade da AMPKα em ratos obesos induzidos por dieta rica em gordura
spellingShingle Efeitos do exercício físico na expressão e atividade da AMPKα em ratos obesos induzidos por dieta rica em gordura
Pauli, José Rodrigo [UNIFESP]
Obesity
Insulin resistance
Physical education and training
title_short Efeitos do exercício físico na expressão e atividade da AMPKα em ratos obesos induzidos por dieta rica em gordura
title_full Efeitos do exercício físico na expressão e atividade da AMPKα em ratos obesos induzidos por dieta rica em gordura
title_fullStr Efeitos do exercício físico na expressão e atividade da AMPKα em ratos obesos induzidos por dieta rica em gordura
title_full_unstemmed Efeitos do exercício físico na expressão e atividade da AMPKα em ratos obesos induzidos por dieta rica em gordura
title_sort Efeitos do exercício físico na expressão e atividade da AMPKα em ratos obesos induzidos por dieta rica em gordura
author Pauli, José Rodrigo [UNIFESP]
author_facet Pauli, José Rodrigo [UNIFESP]
Ropelle, Eduardo Rochete
Cintra, Dennys Esper
Souza, Claudio Teodoro de
author_role author
author2 Ropelle, Eduardo Rochete
Cintra, Dennys Esper
Souza, Claudio Teodoro de
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Pauli, José Rodrigo [UNIFESP]
Ropelle, Eduardo Rochete
Cintra, Dennys Esper
Souza, Claudio Teodoro de
dc.subject.por.fl_str_mv Obesity
Insulin resistance
Physical education and training
topic Obesity
Insulin resistance
Physical education and training
description Introduction: High-fat diet is a special risk factor in the development of insulin resistance and type 2 diabetes. Objective: To investigate the effects of physical exercise on the AMPK expression and activity in high-fat diet induced obese rats. Methods: Wistar rats were randomly divided into four groups and received either a rat maintenance diet (control group) or an isocaloric high-fat diet (HFD) (sedentary groups and exercised groups) for four months. Two different exercise protocols were utilized: acute or chronic swimming exercise. Insulin tolerance test was performed to estimate whole-body insulin sensitivity. AMPK alpha and GLUT4 as well as pAMPK alpha and pACC of rats' skeletal muscle levels were determined using Western blot. Results: Insulin tolerance test revealed a significantly impaired insulin action after HFDt feeding, indicating high-fat induced insulin resistance when compared to control group. Four months of HFD treatment induced to significant decrease of AMPKa (2.2-fold) and GLUT4 (2.5-fold) protein contents and also of p-AMPK alpha (2.4-fold) and p-ACC (2.5-fold) in sedentary rats' skeletal muscle when compared with the control group. Both exercise protocols resulted in increase of AMPKa and ACC phosphorylation and increase in insulin sensitivity, while chronic physical exercise alone provoked increase in these proteins expression (p < 0.05). Conclusion: High-fat feeding impairs AMPKa activity, while AMPKa activation by physical exercise improves insulin resistance, thus indicating that obese rats normally have the AMPK pathway preserved.
publishDate 2009
dc.date.none.fl_str_mv 2009-03-01
2018-06-18T11:35:44Z
2018-06-18T11:35:44Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://dx.doi.org/10.1590/S1517-86922009000200003
Revista Brasileira De Medicina Do Esporte. Sao Paulo Sp: Soc Brasileira Med Esporte, v. 15, n. 2, p. 98-103, 2009.
10.1590/S1517-86922009000200003
S1517-86922009000200003.pdf
1517-8692
S1517-86922009000200003
https://repositorio.unifesp.br/handle/11600/45256
WOS:000266133400003
url https://dx.doi.org/10.1590/S1517-86922009000200003
https://repositorio.unifesp.br/handle/11600/45256
identifier_str_mv Revista Brasileira De Medicina Do Esporte. Sao Paulo Sp: Soc Brasileira Med Esporte, v. 15, n. 2, p. 98-103, 2009.
10.1590/S1517-86922009000200003
S1517-86922009000200003.pdf
1517-8692
S1517-86922009000200003
WOS:000266133400003
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Revista Brasileira De Medicina Do Esporte
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 98-103
application/pdf
dc.publisher.none.fl_str_mv Soc Brasileira Med Esporte
publisher.none.fl_str_mv Soc Brasileira Med Esporte
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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