Fosi is a new integron-associated gene cassette encoding reduced susceptibility to fosfomycin
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| Texto Completo: | http://dx.doi.org/10.1128/AAC.02437-15 http://repositorio.unifesp.br/handle/11600/49623 |
Resumo: | In this work, we demonstrate that the fosI gene encodes a predicted small protein with 134 amino acids and determines reduced susceptibility to fosfomycin. It raised the MIC from 0.125 to 6 mu g/ml when the pBRA100 plasmid was introduced into Escherichia coli TOP10 and to 16 mu g/ml when the gene was cloned into the pBC_SK(-) vector and expressed in E. coli TOP10. |
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Fosi is a new integron-associated gene cassette encoding reduced susceptibility to fosfomycinUrinary-Tract-InfectionsEscherichia-ColiMycobacterium-TuberculosisKlebsiella-PneumoniaeResistance GeneOutcomesEpidemiologyMechanismPlasmidsBacteriaIn this work, we demonstrate that the fosI gene encodes a predicted small protein with 134 amino acids and determines reduced susceptibility to fosfomycin. It raised the MIC from 0.125 to 6 mu g/ml when the pBRA100 plasmid was introduced into Escherichia coli TOP10 and to 16 mu g/ml when the gene was cloned into the pBC_SK(-) vector and expressed in E. coli TOP10.School of Medicine, Infectious Diseases Department, University of São Paulo, São Paulo, SP, BrazilClinical Analysis and Toxicology Department, School of Pharmacy, University of São Paulo, São Paulo, SP, BrazilDepartamento de Microbiologia e Imunologia, Universidade Federal de São Paulo, São Paulo, SP, BrazilSchool of Medicine, Otorhinolaryngology Lab/LIM32, Hospital das Clínicas, University of São Paulo, São Paulo, SP, BrazilUniversité Limoges, INSERM, CHU Limoges, UMR_S 1092, Limoges, FranceFleury Medicine and Health, Microbiology Department, São Paulo, SP, BrazilDepartamento de Microbiologia e Imunologia, Universidade Federal de São Paulo, São Paulo, SP, BrazilWeb of ScienceSao Paulo Research Foundation (FAPESP) [2010/00807-3]FAPESP: 2010/00807-3Amer soc microbiology2019-01-21T10:30:10Z2019-01-21T10:30:10Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion686-688http://dx.doi.org/10.1128/AAC.02437-15Antimicrobial Agents And Chemotherapy. Washington, v. 60, n. 1, p. 686-688, 2016.10.1128/AAC.02437-150066-4804http://repositorio.unifesp.br/handle/11600/49623WOS:000369154600094engAntimicrobial Agents And Chemotherapyinfo:eu-repo/semantics/openAccessPelegrino, Karla de OliveiraCampos, Juliana CoutinhoFerreira Sampaio, Suely CarlosLezirovitz, KarinaSeco, Bruna MaraPereira, Mayne de Oliveirada Costa Rocha, Darlan AugustoJove, ThomasNicodemo, Antonio CarlosMello Sampaio, Jorge Luizreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2022-02-08T11:54:12Zoai:repositorio.unifesp.br/:11600/49623Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652022-02-08T11:54:12Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
Fosi is a new integron-associated gene cassette encoding reduced susceptibility to fosfomycin |
| title |
Fosi is a new integron-associated gene cassette encoding reduced susceptibility to fosfomycin |
| spellingShingle |
Fosi is a new integron-associated gene cassette encoding reduced susceptibility to fosfomycin Pelegrino, Karla de Oliveira Urinary-Tract-Infections Escherichia-Coli Mycobacterium-Tuberculosis Klebsiella-Pneumoniae Resistance Gene Outcomes Epidemiology Mechanism Plasmids Bacteria |
| title_short |
Fosi is a new integron-associated gene cassette encoding reduced susceptibility to fosfomycin |
| title_full |
Fosi is a new integron-associated gene cassette encoding reduced susceptibility to fosfomycin |
| title_fullStr |
Fosi is a new integron-associated gene cassette encoding reduced susceptibility to fosfomycin |
| title_full_unstemmed |
Fosi is a new integron-associated gene cassette encoding reduced susceptibility to fosfomycin |
| title_sort |
Fosi is a new integron-associated gene cassette encoding reduced susceptibility to fosfomycin |
| author |
Pelegrino, Karla de Oliveira |
| author_facet |
Pelegrino, Karla de Oliveira Campos, Juliana Coutinho Ferreira Sampaio, Suely Carlos Lezirovitz, Karina Seco, Bruna Mara Pereira, Mayne de Oliveira da Costa Rocha, Darlan Augusto Jove, Thomas Nicodemo, Antonio Carlos Mello Sampaio, Jorge Luiz |
| author_role |
author |
| author2 |
Campos, Juliana Coutinho Ferreira Sampaio, Suely Carlos Lezirovitz, Karina Seco, Bruna Mara Pereira, Mayne de Oliveira da Costa Rocha, Darlan Augusto Jove, Thomas Nicodemo, Antonio Carlos Mello Sampaio, Jorge Luiz |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Pelegrino, Karla de Oliveira Campos, Juliana Coutinho Ferreira Sampaio, Suely Carlos Lezirovitz, Karina Seco, Bruna Mara Pereira, Mayne de Oliveira da Costa Rocha, Darlan Augusto Jove, Thomas Nicodemo, Antonio Carlos Mello Sampaio, Jorge Luiz |
| dc.subject.por.fl_str_mv |
Urinary-Tract-Infections Escherichia-Coli Mycobacterium-Tuberculosis Klebsiella-Pneumoniae Resistance Gene Outcomes Epidemiology Mechanism Plasmids Bacteria |
| topic |
Urinary-Tract-Infections Escherichia-Coli Mycobacterium-Tuberculosis Klebsiella-Pneumoniae Resistance Gene Outcomes Epidemiology Mechanism Plasmids Bacteria |
| description |
In this work, we demonstrate that the fosI gene encodes a predicted small protein with 134 amino acids and determines reduced susceptibility to fosfomycin. It raised the MIC from 0.125 to 6 mu g/ml when the pBRA100 plasmid was introduced into Escherichia coli TOP10 and to 16 mu g/ml when the gene was cloned into the pBC_SK(-) vector and expressed in E. coli TOP10. |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016 2019-01-21T10:30:10Z 2019-01-21T10:30:10Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1128/AAC.02437-15 Antimicrobial Agents And Chemotherapy. Washington, v. 60, n. 1, p. 686-688, 2016. 10.1128/AAC.02437-15 0066-4804 http://repositorio.unifesp.br/handle/11600/49623 WOS:000369154600094 |
| url |
http://dx.doi.org/10.1128/AAC.02437-15 http://repositorio.unifesp.br/handle/11600/49623 |
| identifier_str_mv |
Antimicrobial Agents And Chemotherapy. Washington, v. 60, n. 1, p. 686-688, 2016. 10.1128/AAC.02437-15 0066-4804 WOS:000369154600094 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Antimicrobial Agents And Chemotherapy |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
686-688 |
| dc.publisher.none.fl_str_mv |
Amer soc microbiology |
| publisher.none.fl_str_mv |
Amer soc microbiology |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
| instname_str |
Universidade Federal de São Paulo (UNIFESP) |
| instacron_str |
UNIFESP |
| institution |
UNIFESP |
| reponame_str |
Repositório Institucional da UNIFESP |
| collection |
Repositório Institucional da UNIFESP |
| repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
| repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
| _version_ |
1814268374997270528 |