Interleukin-6-174 G/C promoter polymorphism and nasal polyposis

• Autor(a) principal: Kosugi, Eduardo Macoto [UNIFESP]
• Data de Publicação: 2009
• Outros Autores: Camargo-Kosugi, Cintia Meirelles de [UNIFESP], Maurice Weckx, Luc Louis [UNIFESP], Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP], Gregorio, Luiz Carlos [UNIFESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://www.rhinologyjournal.com/Rhinology_issues/807.pdf; http://repositorio.unifesp.br/handle/11600/45527
• topic: nasal polyps; interleukin-6; genetic polymorphism; restriction fragment length polymorphism; single nucleotide polymorphism

Introduction: Nasal polyposis is a chronic disease with unknown etiopathogenesis, although inflammatory mechanisms seem to play a role. One of several inflammatory mediators linked to nasal polyposis is Interleukin-6, which has a single. nucleotide polymorphism -174 G/C that seems to promote an inflammatory reaction.Objective: To compare the prevalence of the -174 G/C single nucleotide polymorphism between a group of patients with nasal polyposis and a control group.Method. Cross-sectional study with two groups (thirty two patients with nasal polyposis and fifty five controls) to investigate the -174 G/C polymorphism in blood samples. Asthma, aspirin intolerance and atopy were main exclusion criteria. IL-6 genotyping was performed using the PCR method with forward primer 5'-ATGCCAAGTGCTGAGTCACTA-3' and reverse primer 5'-GGAAAATCCCACATTTGATA-3', amplifying a 226-bp DNA fragment that contained the 174 position. The amplified fragment can be cleaved by restriction enzyme NlaIII when the -174 position presented the C allele in two fragments of 117 and 109-bp, visualized by electrophoresis, classifying participants in GG, GC and CC.Results: In the nasal polyposis group, 65.62% of the patients had the GG genotype, while in the control group only 41.82% had the GG genotype, a statistically significant difference, with an odds ratio of 2.65.Conclusion: The -174 GG genotype was found more frequency in nasal polyposis patients than in controls, when asthma, aspirin intolerance and atopy were excluded.