Detection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a pcr-rflp assay
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.seer.ufu.br/index.php/biosciencejournal/article/view/7041 http://hdl.handle.net/11449/16026 |
Resumo: | Interleukin 8 (IL-8) is a chemokine that acts as a potent chemoattractant for neutrophils. Single nucleotide polymorphisms ( SNPs) in the human IL8 gene have been investigated in many disease association studies. We have developed a different PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment of Length Polymorphism) assay for genotyping the SNP (rs2227307) in the IL8 gene. This method was used for typing 147 white healthy Brazilian individuals, whose DNA was obtained from buccal epithelial cells and extracted with phenol: chloroform: isoamyl alcohol. Genomic DNA was amplified by PCR using a conventional thermal cycler. The PCR products ( 573 bp) were submitted to RFLP reactions. The RFLP fragments were analyzed in a 4% agarose gel stained with ethidium bromide. The genotype distribution observed in this study was consistent with the assumption of Hardy-Weinberg equilibrium and was similar (p=0.30) to those reported for other white populations in the SNP Database of the National Center for Biotechnology Information (NCBI). Because the PCR-RFLP method presented here was efficient, low cost, reproducible and convenient for laboratories with a limited level of technology worldwide, it should be useful for genotyping in case-control association or population genetic studies. |
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Detection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a pcr-rflp assaySingle Nucleotide PolymorphismInterleukin 8PCR-RFLP assayInterleukin 8 (IL-8) is a chemokine that acts as a potent chemoattractant for neutrophils. Single nucleotide polymorphisms ( SNPs) in the human IL8 gene have been investigated in many disease association studies. We have developed a different PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment of Length Polymorphism) assay for genotyping the SNP (rs2227307) in the IL8 gene. This method was used for typing 147 white healthy Brazilian individuals, whose DNA was obtained from buccal epithelial cells and extracted with phenol: chloroform: isoamyl alcohol. Genomic DNA was amplified by PCR using a conventional thermal cycler. The PCR products ( 573 bp) were submitted to RFLP reactions. The RFLP fragments were analyzed in a 4% agarose gel stained with ethidium bromide. The genotype distribution observed in this study was consistent with the assumption of Hardy-Weinberg equilibrium and was similar (p=0.30) to those reported for other white populations in the SNP Database of the National Center for Biotechnology Information (NCBI). Because the PCR-RFLP method presented here was efficient, low cost, reproducible and convenient for laboratories with a limited level of technology worldwide, it should be useful for genotyping in case-control association or population genetic studies.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Estadual Paulista, UNESP, Fac Odontol Araraquara FOAr, Araraquara, SP, BrazilUNESP, Inst Quim Araraquara, Araraquara, SP, BrazilUNESP, FOAr, Disciplina Periodontia, Araraquara, SP, BrazilUNESP, FOAr, Disciplina Genet Humana, Araraquara, SP, BrazilUniv Estadual Paulista, UNESP, Fac Odontol Araraquara FOAr, Araraquara, SP, BrazilUNESP, Inst Quim Araraquara, Araraquara, SP, BrazilUNESP, FOAr, Disciplina Periodontia, Araraquara, SP, BrazilUNESP, FOAr, Disciplina Genet Humana, Araraquara, SP, BrazilFAPESP: 03/10424-0FAPESP: 05/03231-7FAPESP: 05/04553-8FAPESP: 06/04492-1Universidade Federal de Uberlândia (UFU)Universidade Estadual Paulista (Unesp)Kim, Yeon Jung [UNESP]Viana, Aline CavalcantiTfaile Corbi, Samia Cruzde Carvalho Curtis, Karen Maria [UNESP]Renzi, RiveltoOrrico, Silvana Regina Perez [UNESP]Cirelli, Joni Augusto [UNESP]Scarel-Caminaga, Raquel Mantuaneli [UNESP]2013-09-30T18:32:04Z2014-05-20T13:45:32Z2013-09-30T18:32:04Z2014-05-20T13:45:32Z2009-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article136-142application/pdfhttp://www.seer.ufu.br/index.php/biosciencejournal/article/view/7041Bioscience Journal. Uberlandia: Universidade Federal de Uberlândia (UFU), v. 25, n. 6, p. 136-142, 2009.1516-3725http://hdl.handle.net/11449/16026WOS:000272788800015WOS000272788800015.pdf73833913192920402628593693450121Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioscience Journal0,303info:eu-repo/semantics/openAccess2024-09-26T15:22:22Zoai:repositorio.unesp.br:11449/16026Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-26T15:22:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Detection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a pcr-rflp assay |
title |
Detection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a pcr-rflp assay |
spellingShingle |
Detection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a pcr-rflp assay Kim, Yeon Jung [UNESP] Single Nucleotide Polymorphism Interleukin 8 PCR-RFLP assay |
title_short |
Detection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a pcr-rflp assay |
title_full |
Detection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a pcr-rflp assay |
title_fullStr |
Detection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a pcr-rflp assay |
title_full_unstemmed |
Detection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a pcr-rflp assay |
title_sort |
Detection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a pcr-rflp assay |
author |
Kim, Yeon Jung [UNESP] |
author_facet |
Kim, Yeon Jung [UNESP] Viana, Aline Cavalcanti Tfaile Corbi, Samia Cruz de Carvalho Curtis, Karen Maria [UNESP] Renzi, Rivelto Orrico, Silvana Regina Perez [UNESP] Cirelli, Joni Augusto [UNESP] Scarel-Caminaga, Raquel Mantuaneli [UNESP] |
author_role |
author |
author2 |
Viana, Aline Cavalcanti Tfaile Corbi, Samia Cruz de Carvalho Curtis, Karen Maria [UNESP] Renzi, Rivelto Orrico, Silvana Regina Perez [UNESP] Cirelli, Joni Augusto [UNESP] Scarel-Caminaga, Raquel Mantuaneli [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Kim, Yeon Jung [UNESP] Viana, Aline Cavalcanti Tfaile Corbi, Samia Cruz de Carvalho Curtis, Karen Maria [UNESP] Renzi, Rivelto Orrico, Silvana Regina Perez [UNESP] Cirelli, Joni Augusto [UNESP] Scarel-Caminaga, Raquel Mantuaneli [UNESP] |
dc.subject.por.fl_str_mv |
Single Nucleotide Polymorphism Interleukin 8 PCR-RFLP assay |
topic |
Single Nucleotide Polymorphism Interleukin 8 PCR-RFLP assay |
description |
Interleukin 8 (IL-8) is a chemokine that acts as a potent chemoattractant for neutrophils. Single nucleotide polymorphisms ( SNPs) in the human IL8 gene have been investigated in many disease association studies. We have developed a different PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment of Length Polymorphism) assay for genotyping the SNP (rs2227307) in the IL8 gene. This method was used for typing 147 white healthy Brazilian individuals, whose DNA was obtained from buccal epithelial cells and extracted with phenol: chloroform: isoamyl alcohol. Genomic DNA was amplified by PCR using a conventional thermal cycler. The PCR products ( 573 bp) were submitted to RFLP reactions. The RFLP fragments were analyzed in a 4% agarose gel stained with ethidium bromide. The genotype distribution observed in this study was consistent with the assumption of Hardy-Weinberg equilibrium and was similar (p=0.30) to those reported for other white populations in the SNP Database of the National Center for Biotechnology Information (NCBI). Because the PCR-RFLP method presented here was efficient, low cost, reproducible and convenient for laboratories with a limited level of technology worldwide, it should be useful for genotyping in case-control association or population genetic studies. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-11-01 2013-09-30T18:32:04Z 2013-09-30T18:32:04Z 2014-05-20T13:45:32Z 2014-05-20T13:45:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.seer.ufu.br/index.php/biosciencejournal/article/view/7041 Bioscience Journal. Uberlandia: Universidade Federal de Uberlândia (UFU), v. 25, n. 6, p. 136-142, 2009. 1516-3725 http://hdl.handle.net/11449/16026 WOS:000272788800015 WOS000272788800015.pdf 7383391319292040 2628593693450121 |
url |
http://www.seer.ufu.br/index.php/biosciencejournal/article/view/7041 http://hdl.handle.net/11449/16026 |
identifier_str_mv |
Bioscience Journal. Uberlandia: Universidade Federal de Uberlândia (UFU), v. 25, n. 6, p. 136-142, 2009. 1516-3725 WOS:000272788800015 WOS000272788800015.pdf 7383391319292040 2628593693450121 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bioscience Journal 0,303 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
136-142 application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Uberlândia (UFU) |
publisher.none.fl_str_mv |
Universidade Federal de Uberlândia (UFU) |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546527393579008 |