Detection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a pcr-rflp assay

Detalhes bibliográficos
Autor(a) principal: Kim, Yeon Jung [UNESP]
Data de Publicação: 2009
Outros Autores: Viana, Aline Cavalcanti, Tfaile Corbi, Samia Cruz, de Carvalho Curtis, Karen Maria [UNESP], Renzi, Rivelto, Orrico, Silvana Regina Perez [UNESP], Cirelli, Joni Augusto [UNESP], Scarel-Caminaga, Raquel Mantuaneli [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.seer.ufu.br/index.php/biosciencejournal/article/view/7041
http://hdl.handle.net/11449/16026
Resumo: Interleukin 8 (IL-8) is a chemokine that acts as a potent chemoattractant for neutrophils. Single nucleotide polymorphisms ( SNPs) in the human IL8 gene have been investigated in many disease association studies. We have developed a different PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment of Length Polymorphism) assay for genotyping the SNP (rs2227307) in the IL8 gene. This method was used for typing 147 white healthy Brazilian individuals, whose DNA was obtained from buccal epithelial cells and extracted with phenol: chloroform: isoamyl alcohol. Genomic DNA was amplified by PCR using a conventional thermal cycler. The PCR products ( 573 bp) were submitted to RFLP reactions. The RFLP fragments were analyzed in a 4% agarose gel stained with ethidium bromide. The genotype distribution observed in this study was consistent with the assumption of Hardy-Weinberg equilibrium and was similar (p=0.30) to those reported for other white populations in the SNP Database of the National Center for Biotechnology Information (NCBI). Because the PCR-RFLP method presented here was efficient, low cost, reproducible and convenient for laboratories with a limited level of technology worldwide, it should be useful for genotyping in case-control association or population genetic studies.
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spelling Detection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a pcr-rflp assaySingle Nucleotide PolymorphismInterleukin 8PCR-RFLP assayInterleukin 8 (IL-8) is a chemokine that acts as a potent chemoattractant for neutrophils. Single nucleotide polymorphisms ( SNPs) in the human IL8 gene have been investigated in many disease association studies. We have developed a different PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment of Length Polymorphism) assay for genotyping the SNP (rs2227307) in the IL8 gene. This method was used for typing 147 white healthy Brazilian individuals, whose DNA was obtained from buccal epithelial cells and extracted with phenol: chloroform: isoamyl alcohol. Genomic DNA was amplified by PCR using a conventional thermal cycler. The PCR products ( 573 bp) were submitted to RFLP reactions. The RFLP fragments were analyzed in a 4% agarose gel stained with ethidium bromide. The genotype distribution observed in this study was consistent with the assumption of Hardy-Weinberg equilibrium and was similar (p=0.30) to those reported for other white populations in the SNP Database of the National Center for Biotechnology Information (NCBI). Because the PCR-RFLP method presented here was efficient, low cost, reproducible and convenient for laboratories with a limited level of technology worldwide, it should be useful for genotyping in case-control association or population genetic studies.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Estadual Paulista, UNESP, Fac Odontol Araraquara FOAr, Araraquara, SP, BrazilUNESP, Inst Quim Araraquara, Araraquara, SP, BrazilUNESP, FOAr, Disciplina Periodontia, Araraquara, SP, BrazilUNESP, FOAr, Disciplina Genet Humana, Araraquara, SP, BrazilUniv Estadual Paulista, UNESP, Fac Odontol Araraquara FOAr, Araraquara, SP, BrazilUNESP, Inst Quim Araraquara, Araraquara, SP, BrazilUNESP, FOAr, Disciplina Periodontia, Araraquara, SP, BrazilUNESP, FOAr, Disciplina Genet Humana, Araraquara, SP, BrazilFAPESP: 03/10424-0FAPESP: 05/03231-7FAPESP: 05/04553-8FAPESP: 06/04492-1Universidade Federal de Uberlândia (UFU)Universidade Estadual Paulista (Unesp)Kim, Yeon Jung [UNESP]Viana, Aline CavalcantiTfaile Corbi, Samia Cruzde Carvalho Curtis, Karen Maria [UNESP]Renzi, RiveltoOrrico, Silvana Regina Perez [UNESP]Cirelli, Joni Augusto [UNESP]Scarel-Caminaga, Raquel Mantuaneli [UNESP]2013-09-30T18:32:04Z2014-05-20T13:45:32Z2013-09-30T18:32:04Z2014-05-20T13:45:32Z2009-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article136-142application/pdfhttp://www.seer.ufu.br/index.php/biosciencejournal/article/view/7041Bioscience Journal. Uberlandia: Universidade Federal de Uberlândia (UFU), v. 25, n. 6, p. 136-142, 2009.1516-3725http://hdl.handle.net/11449/16026WOS:000272788800015WOS000272788800015.pdf73833913192920402628593693450121Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioscience Journal0,303info:eu-repo/semantics/openAccess2024-09-26T15:22:22Zoai:repositorio.unesp.br:11449/16026Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-26T15:22:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Detection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a pcr-rflp assay
title Detection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a pcr-rflp assay
spellingShingle Detection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a pcr-rflp assay
Kim, Yeon Jung [UNESP]
Single Nucleotide Polymorphism
Interleukin 8
PCR-RFLP assay
title_short Detection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a pcr-rflp assay
title_full Detection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a pcr-rflp assay
title_fullStr Detection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a pcr-rflp assay
title_full_unstemmed Detection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a pcr-rflp assay
title_sort Detection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a pcr-rflp assay
author Kim, Yeon Jung [UNESP]
author_facet Kim, Yeon Jung [UNESP]
Viana, Aline Cavalcanti
Tfaile Corbi, Samia Cruz
de Carvalho Curtis, Karen Maria [UNESP]
Renzi, Rivelto
Orrico, Silvana Regina Perez [UNESP]
Cirelli, Joni Augusto [UNESP]
Scarel-Caminaga, Raquel Mantuaneli [UNESP]
author_role author
author2 Viana, Aline Cavalcanti
Tfaile Corbi, Samia Cruz
de Carvalho Curtis, Karen Maria [UNESP]
Renzi, Rivelto
Orrico, Silvana Regina Perez [UNESP]
Cirelli, Joni Augusto [UNESP]
Scarel-Caminaga, Raquel Mantuaneli [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Kim, Yeon Jung [UNESP]
Viana, Aline Cavalcanti
Tfaile Corbi, Samia Cruz
de Carvalho Curtis, Karen Maria [UNESP]
Renzi, Rivelto
Orrico, Silvana Regina Perez [UNESP]
Cirelli, Joni Augusto [UNESP]
Scarel-Caminaga, Raquel Mantuaneli [UNESP]
dc.subject.por.fl_str_mv Single Nucleotide Polymorphism
Interleukin 8
PCR-RFLP assay
topic Single Nucleotide Polymorphism
Interleukin 8
PCR-RFLP assay
description Interleukin 8 (IL-8) is a chemokine that acts as a potent chemoattractant for neutrophils. Single nucleotide polymorphisms ( SNPs) in the human IL8 gene have been investigated in many disease association studies. We have developed a different PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment of Length Polymorphism) assay for genotyping the SNP (rs2227307) in the IL8 gene. This method was used for typing 147 white healthy Brazilian individuals, whose DNA was obtained from buccal epithelial cells and extracted with phenol: chloroform: isoamyl alcohol. Genomic DNA was amplified by PCR using a conventional thermal cycler. The PCR products ( 573 bp) were submitted to RFLP reactions. The RFLP fragments were analyzed in a 4% agarose gel stained with ethidium bromide. The genotype distribution observed in this study was consistent with the assumption of Hardy-Weinberg equilibrium and was similar (p=0.30) to those reported for other white populations in the SNP Database of the National Center for Biotechnology Information (NCBI). Because the PCR-RFLP method presented here was efficient, low cost, reproducible and convenient for laboratories with a limited level of technology worldwide, it should be useful for genotyping in case-control association or population genetic studies.
publishDate 2009
dc.date.none.fl_str_mv 2009-11-01
2013-09-30T18:32:04Z
2013-09-30T18:32:04Z
2014-05-20T13:45:32Z
2014-05-20T13:45:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.seer.ufu.br/index.php/biosciencejournal/article/view/7041
Bioscience Journal. Uberlandia: Universidade Federal de Uberlândia (UFU), v. 25, n. 6, p. 136-142, 2009.
1516-3725
http://hdl.handle.net/11449/16026
WOS:000272788800015
WOS000272788800015.pdf
7383391319292040
2628593693450121
url http://www.seer.ufu.br/index.php/biosciencejournal/article/view/7041
http://hdl.handle.net/11449/16026
identifier_str_mv Bioscience Journal. Uberlandia: Universidade Federal de Uberlândia (UFU), v. 25, n. 6, p. 136-142, 2009.
1516-3725
WOS:000272788800015
WOS000272788800015.pdf
7383391319292040
2628593693450121
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Bioscience Journal
0,303
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 136-142
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Uberlândia (UFU)
publisher.none.fl_str_mv Universidade Federal de Uberlândia (UFU)
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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